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2 results on '"ATP binding cassette protein B4"'

1. Bezafibrate stimulates canalicular localization of NBD-labeled PC in HepG2 cells by PPARα-mediated redistribution of ABCB4

Author

Junichi Shoda, Yoichi Inada, Atsutoshi Tsuji, Hiroshi Kusama, Tetsuya Ueda, Tadashi Ikegami, Hiroshi Suzuki, Yuichi Sugiyama, David E. Cohen, and Naomi Tanaka

Subjects
ATP binding cassette protein B4, human hepatocyte, multidrug-resistance 3 P-glycoprotein, subcellular localization, functional activity, nuclear hormone receptor, Biochemistry, QD415-436
Abstract

Fibrates, including bezafibrate (BF), upregulate the expression of ATP binding cassette protein B4 (ABCB4) through gene transcription in mice. To determine the effects of BF on the expression levels of ABCB4 and on the stimulation of biliary phosphatidylcholine (PC) transport in human HepG2 hepatoblastoma cells, mRNA and protein levels as well as subcellular localization were investigated in the cells treated with BF. The canalicular accumulation of a fluorescent PC was assessed by confocal laser scanning microscopy. Treatment with 300 μmol/l BF for 24 h increased levels of ABCB4 mRNA but not protein by up to 151%. BF caused redistribution of ABCB4 into pseudocanaliculi formed between cells. In association with this redistribution, BF accelerated the accumulation of fluorescent PC in bile canaliculi (up to 163% of that in nontreated cells). Suppression of peroxisome proliferator-activated receptor α (PPARα) expression by either a small interfering RNA duplex or morpholino antisense oligonucleotide attenuated the BF-induced redistribution of ABCB4.These findings suggest that BF may enhance the capacity of human hepatocytes to direct PC into bile canaliculi via PPARα-mediated redistribution of ABCB4 to the canalicular membrane. This provides a rationale for the use of BF to improve cholestasis and/or cholangitis that is attributable to hypofunction of ABCB4.

Published
2004
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2. Bezafibrate stimulates canalicular localization of NBD-labeled PC in HepG2 cells by PPARα-mediated redistribution of ABCB4

Author

Atsutoshi Tsuji, Yoichi Inada, Tetsuya Ueda, Junichi Shoda, David E. Cohen, Hiroshi Suzuki, Naomi Tanaka, Yuichi Sugiyama, Hiroshi Kusama, and Tadashi Ikegami

Subjects
Small interfering RNA, ATP Binding Cassette Transporter, Subfamily B, QD415-436, Biology, Biochemistry, Endocrinology, Cholestasis, Downregulation and upregulation, Cell Line, Tumor, subcellular localization, medicine, Humans, PPAR alpha, Tissue Distribution, RNA, Messenger, Receptor, human hepatocyte, Messenger RNA, ATP binding cassette protein B4, Bezafibrate, Bile Canaliculi, multidrug-resistance 3 P-glycoprotein, Cell Biology, ABCB4, medicine.disease, Subcellular localization, Molecular biology, 4-Chloro-7-nitrobenzofurazan, nuclear hormone receptor, Hepatocytes, Phosphatidylcholines, functional activity, ATP-Binding Cassette Transporters, medicine.drug
Abstract

Fibrates, including bezafibrate (BF), upregulate the expression of ATP binding cassette protein B4 (ABCB4) through gene transcription in mice. To determine the effects of BF on the expression levels of ABCB4 and on the stimulation of biliary phosphatidylcholine (PC) transport in human HepG2 hepatoblastoma cells, mRNA and protein levels as well as subcellular localization were investigated in the cells treated with BF. The canalicular accumulation of a fluorescent PC was assessed by confocal laser scanning microscopy. Treatment with 300 micromol/l BF for 24 h increased levels of ABCB4 mRNA but not protein by up to 151%. BF caused redistribution of ABCB4 into pseudocanaliculi formed between cells. In association with this redistribution, BF accelerated the accumulation of fluorescent PC in bile canaliculi (up to 163% of that in nontreated cells). Suppression of peroxisome proliferator-activated receptor alpha (PPARalpha) expression by either a small interfering RNA duplex or morpholino antisense oligonucleotide attenuated the BF-induced redistribution of ABCB4. These findings suggest that BF may enhance the capacity of human hepatocytes to direct PC into bile canaliculi via PPARalpha-mediated redistribution of ABCB4 to the canalicular membrane. This provides a rationale for the use of BF to improve cholestasis and/or cholangitis that is attributable to hypofunction of ABCB4.

Published
2004

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