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Your search keyword '"ANR-12-BS08-0020,SynchroKin,Mise en place d'une technique expérimentale pour la mesure de données cinétiques et mécanistiques utilisant la radiation synchrotron SOLEIL(2012)"' showing total 3 results
Start Over Author "ANR-12-BS08-0020,SynchroKin,Mise en place d'une technique expérimentale pour la mesure de données cinétiques et mécanistiques utilisant la radiation synchrotron SOLEIL(2012)"
3 results on '"ANR-12-BS08-0020,SynchroKin,Mise en place d'une technique expérimentale pour la mesure de données cinétiques et mécanistiques utilisant la radiation synchrotron SOLEIL(2012)"'

1. VUV photoionization of the CH 2 NC radical: adiabatic ionization energy and cationic vibrational mode wavenumber determinations

Author

Oliver J. Harper, Sebastian Hartweg, Séverine Boyé-Péronne, Gustavo A. Garcia, Jean-Claude Guillemin, Bérenger Gans, Jean-Christophe Loison, Université de Bordeaux, ISM, UMR CNRS 5255, Talence, 33405, France, Institut des Sciences Moléculaires d'Orsay (ISMO), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), European Synchrotron Radiation Facility (ESRF), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), This work was performed on the DESIRS beamline under proposal number 20181133. We acknowledge SOLEIL for provisionof synchrotron radiation facilities and the DESIRS beamline staff for their assistance. This work received financial support fromthe French Agence Nationale de la Recherche (ANR) under grant ANR-12-BS08-0020-02 (project SYNCHROKIN). This work wassupported by the Programme National Physique et Chimie du Milieu Interstellaire (PCMI) of CNRS/INSU with INC/INP co-fundedby CEA and CNES. J.-C.G. thanks the CNES for a grant., ANR-12-BS08-0020,SynchroKin,Mise en place d'une technique expérimentale pour la mesure de données cinétiques et mécanistiques utilisant la radiation synchrotron SOLEIL(2012), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Physics, [SDU.ASTR]Sciences of the Universe [physics]/Astrophysics [astro-ph], General Physics and Astronomy, 02 engineering and technology, Photoionization, Electron, 021001 nanoscience & nanotechnology, 01 natural sciences, 7. Clean energy, Spectral line, Ion, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Ab initio quantum chemistry methods, Molecular vibration, 0103 physical sciences, Physics::Atomic and Molecular Clusters, Physical and Theoretical Chemistry, Atomic physics, Ionization energy, Physics::Chemical Physics, 0210 nano-technology, 010303 astronomy & astrophysics, Excitation, ComputingMilieux_MISCELLANEOUS
Abstract

The photoelectron spectroscopy of CH2NC (isocyanomethyl) radical species is investigated for the first time between 9.3 and 11.2 eV in the vicinity of the first photoionizing transition X+1A1 ← X 2B1. The experiment combines a microwave discharge flow-tube reactor to produce the radicals through the CH3NC + F → CH2NC + HF reaction, a VUV synchrotron radiation excitation, and a double imaging electron/ion coincidence spectrometer which allows the recording of mass-selected threshold photoelectron spectra. Assignment of the observed vibrational structure of the CH2NC+ cation is guided by ab initio calculations and Franck-Condon simulations. From the experimental spectrum, the first adiabatic ionization energy of the CH2NC radical is measured as 9.439(6) eV. Fundamental wavenumbers are determined for several vibrational modes of the cation [small nu, Greek, tilde]1+(CH2 symmetric stretch) = 2999(80) cm-1, [small nu, Greek, tilde]2+(NC stretch) = 1925(40) cm-1, [small nu, Greek, tilde]4+(H2C-N stretch) = 1193(40) cm-1, [small nu, Greek, tilde]6+(CNC out-of-plane bend) = 237(50) cm-1, and [small nu, Greek, tilde]8+(CH2 rock) = 1185(60) cm-1.

Published
2020

2. Origin band of the first photoionizing transition of hydrogen isocyanide

Author

Alfredo Aguado, Séverine Boyé-Péronne, Bérenger Gans, Stephen T. Pratt, Octavio Roncero, Gustavo A. Garcia, Jean-Claude Guillemin, Jean-Christophe Loison, Centre National D'Etudes Spatiales (France), Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Agence Nationale de la Recherche (France), European Commission, Department of Energy (US), Centre National de la Recherche Scientifique (France), Gans, B. [0000-0001-9658-2436], Boyé-Péronne, S. [0000-0003-0579-9571], Guillemin, Jean-Claude [0000-0002-2929-057X], Aguado, Alfredo [0000-0002-4270-1046], Roncero, Octavio [0000-0002-8871-4846], Loison, Jean-Christophe [0000-0001-8063-8685], Institut des Sciences Moléculaires d'Orsay (ISMO), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Spanish National Research Council [Madrid] (CSIC), Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1 (UB)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-12-BS08-0020-02, Agence Nationale de la Recherche, Centre National d’Etudes Spatiales, FP/2007-2013, Seventh Framework Programme, 610256, European Research Council, DE-AC02-06CH11357, Basic Energy Sciences, ANR-12-BS08-0020,SynchroKin,Mise en place d'une technique expérimentale pour la mesure de données cinétiques et mécanistiques utilisant la radiation synchrotron SOLEIL(2012), European Project: 610256,EC:FP7:ERC,ERC-2013-SyG,NANOCOSMOS(2014), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Gans, B., Boyé-Péronne, S., Guillemin, Jean-Claude, Aguado, Alfredo, Roncero, Octavio, and Loison, Jean-Christophe

Subjects
Materials science, Photoemission spectroscopy, General Physics and Astronomy, 02 engineering and technology, Photoionization, Photon energy, 010402 general chemistry, 7. Clean energy, 01 natural sciences, Article, chemistry.chemical_compound, Ab initio quantum chemistry methods, Physics::Atomic and Molecular Clusters, [CHIM]Chemical Sciences, Physics::Atomic Physics, Physical and Theoretical Chemistry, Astrophysics::Galaxy Astrophysics, Hydrogen isocyanide, 021001 nanoscience & nanotechnology, Potential energy, 0104 chemical sciences, chemistry, 13. Climate action, Ionization energy, Atomic physics, 0210 nano-technology, Ground state
Abstract

8 pags., 5 figs., 4 tabs., The photoelectron spectrum of the XΣ → XΣ ionizing transition of hydrogen isocyanide (HNC) is measured for the first time at a fixed photon energy (13 eV). The assignment of the spectrum is supported by wave-packet calculations simulating the photoionization transition spectrum and using ab initio calculations of the potential energy surfaces for the three lowest electronic states of the cation. The photoelectron spectrum allows the retrieval of the fundamental of the CN stretching mode of the cationic ground state ( = 2260 ± 80 cm) and the adiabatic ionization energy of hydrogen isocyanide: IE(HNC) = 12.011 ± 0.010 eV, which is far below that of HCN (IE(HCN) = 13.607 eV). In light of this latter result, the thermodynamics of the HCN/HNC isomers is discussed and a short summary of the values available in the literature is given., This work received financial support from the French Agence Nationale de la Recherche (ANR) under Grant No. ANR-12-BS08-0020-02 (project SYNCHROKIN) and the CNRS program “Physique et Chimie du Milieu Interstellaire” (PCMI) co-funded by the Centre National d'Etudes Spatiales (CNES). A. A. and O. R. acknowledge support by the Ministerio de Economía e Innovación under grants No. FIS2014-52172-C2 and FIS2017-83473-C2 and from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 610256 (NANOCOSMOS). Work by S. T. P. was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences respectively under contract No. DE-AC02-06CH11357. J.-C. G. thanks the Centre National d'Etudes Spatiales (CNES) for financial support.

Published
2019

3. Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach

Author

Philippe Delepelaire, Gisele Cardoso de Amorim, Rémi Fronzes, Javier Pérez, Catherine Simenel, Gérard Pehau-Arnaudet, Muriel Delepierre, Idir Malki, Olga Roudenko, Alexandros Koutsioubas, Francesca Gubellini, Nadia Izadi-Pruneyre, Halina Wójtowicz, Ada Prochnicka-Chalufour, Résonance Magnétique Nucléaire des Biomolécules, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Centre de Recherche et Innovation Technologique (CITECH), Institut Pasteur [Paris] (IP), Biologie Structurale de la Sécrétion Bactérienne, Jülich Supercomputing Centre (JSC), Forschungszentrum Jülich GmbH | Centre de recherche de Juliers, Helmholtz-Gemeinschaft = Helmholtz Association-Helmholtz-Gemeinschaft = Helmholtz Association, Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), This work was funded by the Institut Pasteur, the Centre National de la Recherche Scientifique (CNRS), two grants from the ANR (Agence Nationale de la Recherche) [grant numbers HEMESTOCKEXCHANGE 12-BSV3-0022-01 and HEMETRANS 08-BLAN-0160], Equipement d'Excellence CACSICE and SOLEIL [grant number 2013448]. H.W. was funded by postdoctoral fellowships from the Mairie de Paris [grant number 2012-209]., We thank Emmanuel Frachon, from the Platform of Recombinant Protein of the Institut Pasteur, for bacterial cell production. We thank Dominique Durand and Patrice Vachette for the first SAXS measurements on HasR. We thank Florence Cordier and Bertrand Raynal for helpful discussions., ANR-12-BSV3-0022,HEMESTOCKEXCHANGE,Hème et porphyrine chez des bactéries modèle: des fonctions aux mécanismes(2012), ANR-08-BLAN-0160,HEMETRANS,Transfert et transport d'hème entre protéines et signalisation transmembranaire(2008), ANR-11-EQPX-0008,CACSICE,Centre d'analyse de systèmes complexes dans les environnements complexes(2011), ANR-12-BS08-0020,SynchroKin,Mise en place d'une technique expérimentale pour la mesure de données cinétiques et mécanistiques utilisant la radiation synchrotron SOLEIL(2012), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris], and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Magnetic Resonance Spectroscopy, transmembrane signalling, Heme, Biology, Crystallography, X-Ray, Biochemistry, 03 medical and health sciences, Bacterial Proteins, Cell surface receptor, bacterial nutrient transporter, HasR, Gene expression, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Molecular Biology, Gene, Research Articles, Serratia marcescens, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], digestive, oral, and skin physiology, Membrane Transport Proteins, haem, Transporter, Cell Biology, Periplasmic space, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Cell biology, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Microscopy, Electron, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 030104 developmental biology, Signalling, Cytoplasm, Signal transduction, integrative approach, Research Article, Protein Binding, Signal Transduction
Abstract

In bacteria, some scarce nutrients are sensed, bound and internalized by their specific transporter. In the present study, using an integrative structural approach, we study HasR, a bacterial haem transporter in both its free and its loaded forms., Bacteria use diverse signalling pathways to adapt gene expression to external stimuli. In Gram-negative bacteria, the binding of scarce nutrients to membrane transporters triggers a signalling process that up-regulates the expression of genes of various functions, from uptake of nutrient to production of virulence factors. Although proteins involved in this process have been identified, signal transduction through this family of transporters is not well understood. In the present study, using an integrative approach (EM, SAXS, X-ray crystallography and NMR), we have studied the structure of the haem transporter HasR captured in two stages of the signalling process, i.e. before and after the arrival of signalling activators (haem and its carrier protein). We show for the first time that the HasR domain responsible for signal transfer: (i) is highly flexible in two stages of signalling; (ii) extends into the periplasm at approximately 70–90 Å (1 Å=0.1 nm) from the HasR β-barrel; and (iii) exhibits local conformational changes in response to the arrival of signalling activators. These features would favour the signal transfer from HasR to its cytoplasmic membrane partners.

Published
2016

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