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Bromus tectorum, a winter annual plant, produces dimorphic diaspores: complex diaspores with multi-awns and simple diaspores with one awn. However, there is no information available about the role of awns and the germination characteristics of dimorphic diaspores. Dispersal germination and awns hygroscopicity of the dimorphic diaspores were assessed. The complex diaspore with multi-awns can easily be dispersed long distances from the mother plant by mammals. The simple diaspores with one awn are tightly attached to the mother plant. Caryopses from the two types of diaspores exhibited non-deep physiological dormancy at maturity, which can be released by dry storage and GA3 treatment. The awns have hygroscopic activity and can move in response to changes in moisture, moving the complex diaspore (the seed) into the soil. The seedling emergence from complex diaspores was significantly higher than those from simple diaspores at all burial depths. Germination of caryopses on the soil surface was poor. The optimal planting depth for both types of diaspores' emergence is 1–2 cm. The distinct characteristics of dimorphic diaspores and the beneficial influence of hygroscopic awns on dispersal, germination, and seedling establishment have significant ecological implications for B. tectorum's successful reproduction in unpredictable cold deserts. [ABSTRACT FROM AUTHOR]

Premise Methods Results Conclusions Obligate fire ephemerals are annual plants that have germination and reproduction cued by fire occurrence, persisting between fire events in a long‐lived soil seed bank. Within these species, gene flow is restricted not only geographically but also temporally because individuals are limited to reproducing with others affected by the same fire event. The patchwork‐like distribution of fires may therefore promote population isolation. In contrast to past fires, the Australian fires of 2019–2020 were of unprecedented extent, providing an opportunity to investigate the landscape genetics of a fire ephemeral, Actinotus forsythii, across multiple populations and to compare it to a common congener, Actinotus helianthi.For both species, we used single nucleotide polymorphisms to infer patterns of population structure and calculate measures of genetic diversity. We also estimated a phylogeny of Actinotus forsythii to understand the differentiation of a geographically isolated population.For A. forsythii, the within‐population diversity (allelic richness = 1.56) was greater, and the among‐population differentiation (FST = 0.30) was lower than that observed for A. helianthi (allelic richness = 1.33, FST = 0.57). Actinotus forsythii had distinct geographic groupings, and a geographically isolated population of this species was genetically highly differentiated.Despite the fire‐dependent, asynchronous gene flow, predicted between site disconnect, and possible within‐site homogeneity, our results suggest that burn mosaic could be influencing gene flow patterns and fire‐triggered mass flowering may promote genetic diversity within Actinotus forsythii. [ABSTRACT FROM AUTHOR]

Introduction: The impacts of climate change can be profound in many ecosystems worldwide, including drylands such as arid and semi-arid scrublands and grasslands. Foundation plants such as shrubs can provide microclimatic refuges for a variety of taxa. These shrubs can directly influence micro6 environmental measures, and indirectly increase the local environmental heterogeneity as a result. We examined the hypothesis that, in comparison to an open gap, foundation shrubs improve the microclimate beneath their canopy and that microclimate is in turn a significant predictor of annual vegetation. The following predictions were made: 1) mean air temperature (NSAT), ground temperature (SGT), and vapour pressure deficit (VPD) will be significantly lower under the shrubs than in the open microsites; 2) shrub canopy size predicts microclimate; 3) site-level aridity estimates and percent shrub cover influence annual plant abundance and richness; and 4) the site13 level mean of NSAT and VPD predict annual plant abundance and richness. Methods: Our study took place in Southwestern California, U.S.A. We used a handheld device with a probe to measure microclimatic variables such as nearsurface air temperature (NSAT), near-surface relative humidity (NSRH), and surface ground temperature (SGT) at the shrub species Ephedra californica and in the open gap, across six sites in California, United States. Air temperature and RH were then used to calculate VPD. The mean number of vascular plant species across each site was also recorded. Results & discussion: Only SGT was significantly reduced under shrub canopies. Canopy volume was not a significant predictor of all three microclimatic variables, demonstrating that even small, low-stature shrubs can have facilitative effects. Furthermore, total shrub cover and aridity at sites significantly predicted mean plant richness and abundance. There were significantly more plants associated with shrubs and there were significantly more species associated with the open. Mean air temperature and VPD at the site-level significantly predicted vegetation abundance and richness, though microsite-level differences were only significant for richness. Foundation shrubs are a focal point of resiliency in dryland ecosystems. Understanding their impact on microclimate can inform us of better management, conservation, and restoration frameworks. [ABSTRACT FROM AUTHOR]

The phenology of temperate perennials, including the timing of vegetative growth and flowering, is well known to be controlled by seasonal dormancy cycles. Dormant structures are known as buds and have specialized covering structures, symplastic isolation from the plant, and often autonomous stores of carbon and nitrogen reserves. In contrast, in annual plants, our current understanding of the control of the timing of flowering focuses on the mechanisms affecting floral initiation, the transition from a vegetative apical meristem to a inflorescence meristem producing flower primordia in place of leaves. Recently we revealed that annual crops in Brassicaceae exhibit chilling-responsive growth control in a manner closely resembling bud dormancy breakage in perennial species. Here I discuss evidence that vernalization in autumn is widespread and further discuss its role in inducing flower bud set prior to winter. I also review evidence that flower bud dormancy has a more widespread role in annual plant flowering time control than previously appreciated. [ABSTRACT FROM AUTHOR]

The relative magnitude of additive genetic vs. residual variation for fitness traits is important in models for predicting the rate of evolution and population persistence in response to changes in the environment. In many annual plants, lifetime reproductive fitness is correlated with end-of-season plant biomass, which can vary significantly from plant to plant in the same population. We measured end-of-season plant biomasses and obtained single nucleotide polymorphism (SNP) genotypes of plants in a dense, natural population of the annual plant species Impatiens capensis with hierarchical size structure. These data were used to estimate the amount of heritable variation for position in the size hierarchy and for plant biomass. Additive genetic variance for a position in the size hierarchy and plant biomass were both significantly different from zero. These results are discussed in relationship to the theory for the heritability of fitness in natural populations and ecological factors that potentially influence heritable variation for fitness in this species. [ABSTRACT FROM AUTHOR]

Consumers can influence the competitive outcomes of prey species in various ways. Modern coexistence theory predicts that consumers can promote prey coexistence by preferably targeting a competitively superior one, thereby reducing fitness differences. However, previous studies yielded mixed conclusions. In this study, we tested the hypothesis that a parasitic annual plant, Cuscuta campestris, facilitates the coexistence of two common annual plants. We performed field surveys and parasitism experiments to parameterize a plant competition dynamics model. The model suggested a competition–defence tradeoff: the legume Lespedeza striata was a better competitor than the grass Setaria faberi, while it was more susceptible to the parasite. Moreover, an empirical host–parasite dynamics model, extended from the plant competition model, predicted their coexistence within broad, biologically reasonable ranges of parameters. This work provides field evidence of the coexisting–promoting role of a parasitic plant, as caused by stabilising feedback between host and parasite densities. [ABSTRACT FROM AUTHOR]

Species‐specific phenological responses to changing climate are reshuffling the timing of species interactions, however we do not fully understand the consequences of these changes for species' population dynamics and community composition.In this study, we experimentally manipulated the timing of germination for five annual plant species from southern California and used pairwise competition experiments and coexistence theory to quantify how phenological shifts may impact species interactions and coexistence.We found that phenological shifts may help promote coexistence when they confer an advantage for competitively inferior species, but in other cases promote dominance by competitively superior species. Earlier germination generally increased species' performance relative to competitors, but the relative changes in intra‐and inter‐specific interactions caused more complex effects on niche and fitness differences. Phenological differences tended to reduce stabilising niche differences for many species pairs and reduced overall coexistence probabilities.Synthesis. While phenological differences among species have typically been considered a form of niche partitioning, it seems increasingly likely that phenological offsets could destabilise species coexistence. The net effects of changing phenology on species coexistence will depend on the complex combinations of effects on intra‐ and inter‐specific interactions, which remain challenging to predict. [ABSTRACT FROM AUTHOR]

Efficient post‐drought recovery of growth and assimilation enables a plant to return to its undisturbed state and functioning. Unlike annual plants, trees suffer not only from the current drought, but also from cumulative impacts of consecutive water stresses which cause adverse legacy effects on survival and performance. This review provides an integrated assessment of ecological, physiological and molecular evidence on the recovery of growth and photosynthesis in trees, with a view to informing the breeding of trees with a better ability to recover from water stress. Suppression of recovery processes can result not only from stress damage but also from a controlled downshift of recovery as part of tree acclimation to water‐limited conditions. In the latter case, recovery processes could potentially be activated by turning off the controlling mechanisms, but several obstacles make this unlikely. Tree phenology, and specifically photoperiodic constraints, can limit post‐drought recovery of growth and photosynthesis, and targeting these constraints may represent a promising way to breed trees with an enhanced ability to recover post‐drought. The mechanisms of photoperiod‐dependent regulation of shoot, secondary and root growth and of assimilation processes are reviewed. Finally, the limitations and trade‐offs of altering the photoperiodic regulation of growth and assimilation processes are discussed. [ABSTRACT FROM AUTHOR]

Erodium cicutarium is an annual herbaceous plant valued for its applications in traditional medicine. However, the chloroplast genome of E. cicutarium has yet to be reported. In this study, we assembled chloroplast genomes of Erodium cicutarium using Illumina sequencing reads. The chloroplast genome was 114,652 bp long, harbored 111 complete genes, and its overall GC content was 39.1%. In Maximum Likelihood (ML) and Bayesian inference (BI) trees, the 13 Erodium species divided into three main clades, with E. cicutarium and E. carvifolium forming a monophyletic group, suggesting a close relationship between the two species. The E. cicutarium cp genome presented in this study lays a good foundation for the Erodium. [ABSTRACT FROM AUTHOR]

Plants are sessile organisms and any changes in environmental factors activate various responses and defense mechanisms. Artemisia plants widely inhabit harsh conditions of arid and semiarid ecosystems. Using two species—a subshrub, Artemisia frigida, and an annual–biennial herb, Artemisia scoparia—the functioning of the antioxidant system of plants in semiarid territories have been examined. The activity of enzymatic antioxidants and the content of non-enzymatic antioxidants in both species as well as the antiradical activity of their extracts have been shown. Although the plants were collected in areas differing in moisture supply, the activity of enzymatic antioxidants and the content of non-enzymatic antioxidants corresponds to their physiological level, within the range of the norm of reaction, in wormwood. Consequently, conditions of differing moisture deficiency do not cause a specific biochemical response at the level of the antioxidant system in the studied species, which confirms their adaptability to these conditions. Meanwhile, A. frigida plants show greater morphological and biochemical plasticity than A. scoparia under changing growth conditions. Both species contain tissue monoterpenoids and sesquiterpenoids, the emission of which provides additional protection against high temperatures and drought. Their composition and contents of phenolic components illustrates the differences in adaptation between perennial and annual plants. [ABSTRACT FROM AUTHOR]

Chlorophyll fluorescence measurement is a quick and efficient tool for plant stress‐level detection. The use of Pulse amplitude modulation (PAM), allows the detection of the plant stress level under field conditions. Over the years, several parameters estimating different parts of the chlorophyll and photosystem response were developed to describe the plant stress level. Despite all fluorescence parameters being based on the same measurements, their relationship remains unclear, and their response to drought stress is significantly influenced by the incoming light intensity. In this study, we use six different annual plants from different families, both C3 and C4 photosynthesis types, to describe the plant response to drought through the fluorescence parameters response (NPQ, Y(NPQ), and qN). To describe the dynamic response to drought, we employed light‐response curves, adapting and fitting an equation for each curve to compare the drought response for each fluorescence parameter. The results demonstrated that the non‐photochemical quenching (NPQ) and the quantum yield of non‐photochemical quenching [Y(NPQ)] maximal values decrease when the PSII functionality (Fv/Fm) is lower than ~0.7. The basal fluorescence level (F0$$ {F}_0 $$ and Fs)$$ {F}_s\Big) $$ remained unaffected by the stress level and stayed stable across the various plants and stress levels. Our results indicate that the response of different stress parameters follows a distinct order under continuous drought. Consequently, monitoring just one parameter during long‐term stress assessments may result in biased analysis outcomes. Incorporating multiple chlorophyll fluorescence parameters offers a more accurate reflection of the plant's stress level. [ABSTRACT FROM AUTHOR]

Contemporary agriculture in many parts of the world consists of annual plant species grown in monoculture, but recently interest has focused on developing perennial, herbaceous species for use in perennial polycultures that offer greater ecosystem services relative to annual monocultures. Because few perennial, herbaceous species were domesticated by early farmers, one of the initial steps in identifying wild, perennial, herbaceous species for potential crop development is understanding patterns of variation and covariation among reproductive traits that underpin yield and vegetative traits. Herbaria can serve as a valuable resource for taxonomically broad surveys of phenotypic variation and covariation for pre-breeding. In this study, we used herbarium specimens to investigate trait variation and covariation in five genera of the legume family (Fabaceae). We addressed two questions: (1) do vegetative and reproductive traits vary as a function of life span (annual vs. perennial species), and are these differences consistent across genera?; and (2) how do vegetative and reproductive traits correlate with one another across lifespan and/or within genera? Reproductive traits and vegetative traits were measured on 535 herbarium specimens representing annual and perennial species of Astragalus, Lupinus, Phaseolus, Strophostyles, and Vigna. While species exhibited intra- and interspecific variation, few morphological differences were observed among annual and perennial species. Correlations among vegetative traits and among reproductive traits were mostly positive when significant, and perennial species generally exhibited more positive correlations between vegetative and reproductive traits than annual species. These data have potential relevance for perennial crop development, and provide further support that herbaria represent a rich, relatively untapped resource of information about plant trait variation and covariation. [ABSTRACT FROM AUTHOR]

Pre‐emergent herbicides can reduce the abundance of invasive annual plants, but they can also harm native plants, particularly annuals or perennial seedlings, including seeds planted during restoration. We assessed the effects of imazapic and indaziflam on invasive target and nontarget native plants in the Great Basin, a region with extensive invasive annual grasses. We tested nontarget effects on native annual forbs in an agricultural field previously used to grow native annual forbs, which contained a large seed bank. We seeded perennial grass (Elymus elymoides) at multiple depths to determine susceptibility and resistance. Herbicides were applied at full and reduced rates to mimic the effect of litter in natural systems. We observed reductions in most non‐native species in all treatments, but also extensive reductions of native annual forbs, although these were offset at lower application rates, and some species (e.g. Amsinckia tessellata and Microsteris gracilis) were less susceptible than others. Herbicides, particularly indaziflam, reduced E. elymoides emergence, but planting seeds at 2–3 cm depths improved emergence, particularly for imazapic, with 15–68% greater emergence than seeds planted at 1 cm. We suggest surveys for native annual forbs and resistant invaders before applying herbicides and field testing to determine whether reduced rates could provide weed control while maintaining annual forbs. We suggest planting E. elymoides at 2–3 cm when applying herbicides, an approach that may be effective for other species. Herbicide use can be an effective tool, but our results indicate that mitigation of nontarget effects will be needed to maintain native plant diversity. [ABSTRACT FROM AUTHOR]

Under global change, the impact of seed banks on evolutionary rescue is uncertain. They buffer plant populations from demographic and genetic stochasticity but extend generation time and can become a reservoir of maladapted alleles. We built analytical and individual-based models to predict the effect of seed banks on the persistence of small annual plant populations facing an abrupt or sustained directional change in uni- or multivariate trait optima. Demogenetic dynamics predict that under most scenarios seed banks increase the lag yet enhance persistence to 200–250 years by absorbing demographic losses. Simulations indicate that the seed bank has a minimal impact on the genetic skew, although we suggest that this result could depend on the fitness component under selection. Our multivariate model reveals that by enlarging and reshaping the G matrix, seed banks can diminish the impact of mutational correlation and even accelerate adaptation under antagonistic pleiotropy relative to populations without a bank. We illustrate how the magnitude of optimum fluctuations, type and degree of optimum change, selection strength, and vital rates are weights that tip the scales determining persistence. Finally, our work highlights that migration from the past is not maladaptative when optimum fluctuations are large enough to create stepping stones to the new optimum. [ABSTRACT FROM AUTHOR]

Plants from Brazilian tropical grasslands of the cerrado biome (also referred to as the Cerrado region) are characterized by the possessing of belowground bud-bearing organs that may be of stem or root origin that enhance the likelihood of surviving a dry season or fire. The root-derived bud bank is referred to when speaking about adventitious sprouting from roots. Root buds or root sprouts allow rapid regrowth after a disturbance or a period of adverse climatic conditions and have the potential to generate clonal populations. Adventitious sprouting from roots is well known to occur in Brazil in perennial species, mainly woody plants. In the present study, we investigate the occurrence of adventitious buds on roots of the annual species Siphanthera arenaria (DC.) Cogn., which is endemic to the Serra do Espinhaço range in Brazil and grows in an area of the cerrado biome. Shoots emerging from the root system of S. arenaria were assessed in a microscopic analysis and classified as reparative-type buds emerging from the cortical parenchyma. The presence of root buds in Siphantera arenaria represents the first record for the Melastomataceae family in the cerrado biome and also the first record of a root bud in an annual species for this biome. [ABSTRACT FROM AUTHOR]

Soybean [Glycine max (L.) Merr.] is an annual plant of the legume family that is ancestral to the Glycine bean (Glycine soja Sieb. & Zucc.). Soybeans are a key component of "Japanese cuisine," which has been registered as a UNESCO Heritage of Intangible Differentiation and has become an essential part of the Japanese diet. Despite being an important crop in Japan's food culture, the self-sufficiency rate of Japan's domestically produced soybean crop has declined considerably to ~24 % for use in food products and ~6% for combined nonfood uses. Although domestic consumption of processed soybean products such as tofu has been increasing annually, imported soybeans have been used as raw materials. From the food security perspective, improving the self-sufficiency rate by promoting the development of soybean varieties that are internationally competitive and of superior quality is a prominent issue. Therefore, the Soybean Subcommittee of the 70th Anniversary Meeting of the Japanese Society for Food Science and Technology invited Dr. Yohei Nanjo of the Crops Research Division of the National Institute of Agrobiological Science in 2023 to give a presentation on the current state of soybean breeding and its future outlook. [ABSTRACT FROM AUTHOR]

Detecting changes in the phenological responses of herbaceous species as a function of predicted climate change is important for forecasting future scenarios for the functioning of dry tropical forests, especially when predicting an increase in the frequency and intensity of extreme droughts. Because of the sensitivity of plants to water availability, our study hypothesizes that if years become drier or wetter, herbaceous plants will synchronously change the onset, duration, and intensity of their vegetative phenophases.We used a historical series of 60 years of precipitation observations for the Caatinga vegetation to define daily average of precipitation for rainy (Twet), median (Tcontrol), and dry (Tdry) years. We simulated past average daily rainfall (Twet, Tcontrol, and Tdry) while growing two herbaceous perennials and two herbaceous annuals. We monitored plant growth and measured the activity (absence or presence) and intensity of vegetative phenophases. We used circular statistical analysis to assess differences between treatments.Our results revealed that leaf production was seasonal but relatively uniform for perennial species and highly seasonal (wet season) for annual species. Simulated dry years induced lower leaf emergence concentrated over a few months in annual species, but this effect was more strongly significant in one of the two perennial species. Both annual and perennial species can experience delayed and less intense leaf abscission during the rainy season in years with below‐average precipitation. In contrast, large voluminous rains in years with above‐average precipitation can accelerate and intensify the process of leaf renewal.If future precipitation reductions occur, the changes in phenological response indicate that the cover of annual and perennial herbaceous species in this study will likely decrease, altering the landscape and functioning of dry tropical forests. However, the potential trade‐offs observed may help populations of these species to persist during years of severe drought in the Caatinga. [ABSTRACT FROM AUTHOR]

Tectona grandis is a vital hardwood tree species with substantial industrial and economic importance. From 2020 to 2021, an unusual leaf blight disease was observed in T. grandis saplings planted in the Nursery of the Central Agroforestry Research Institute (CAFRI), Jhansi, India. Disease incidence was recorded as 50%–70% with 40%–82% severity over two consecutive years on over 1000 saplings. The leaf blight disease was extensive, leading to defoliation of young leaves and mortality of teak saplings in the nursery. The pathogen was identified as Alternaria alternata following standard laboratory procedures and sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA). The pathogenicity of two isolates, A. alternata TgAa1 and TgAa2, was proven under greenhouse conditions. Phylogenetic analysis revealed the close association of TgAa1 and TgAa2 with other pathogenic A. alternata strains in the NCBI database. A. alternata is considered a severe foliar pathogen, known to cause leaf spot and leaf blight diseases in many annual and perennial plant species. Correct diagnosis and prevention are emphasized to contain the spread of teak leaf blight to other regions in India. This is the first report of A. alternata causing Tectona grandis leaf blight in the Bundelkhand region of India. [ABSTRACT FROM AUTHOR]

San Fernando Valley spineflower (Chorizanthe parryi var. fernandina [S. Watson] Jeps.) (Polygonaceae) is an herbaceous annual plant, endemic to California, and until rediscovered in 1999 had been thought to be extinct for almost seven decades. Historically documented at 10 locations, it currently persists at 2, separated by approximately 27 km. State listed as endangered, a description of its genetic diversity and structure is of conservation interest. After determining a lack of variation in ploidy, we examined genetic variation from samples within both populations: a common garden study for potentially adaptive genetic variation in selected growth and phenological traits and analysis of single nucleotide polymorphisms identified through restriction-site associated DNA sequencing. Both measures indicated that this highly restricted taxon nevertheless harbors substantial levels of genetic diversity and has significant between- and within-population genetic structure. Combining approaches from population genomics and common garden studies provided more insight into the patterns and basis of genetic diversity than is typical for studies of non-model species. Although local adaptation was not specifically studied (i.e., via reciprocal transplant studies), the differences determined from these two independent lines of evidence indicate that mixing gene pools between populations is not recommended at this time. Further, with significant differences revealed among subpopulations, we caution against mixing genotypes across subpopulations for the most part, and without much more evidence that this would not pose a risk of outbreeding depression. The importance of supporting pollinator health and diversity is highlighted. With genetic diversity—particularly with an annual species—being dynamic, fluctuating with the usual processes and with contributions from the soil seedbank, we recommend periodic resampling to monitor genetic diversity and structure. Climate change is anticipated to contribute to this variability. [ABSTRACT FROM AUTHOR]

Pyankovia brachiata (Pall.) Akhani & Roalson 2007, is an annual plant belonging to the genus Pyankovia, family Amaranthaceae, which is widely distributed in the inland deserts of Northwest China. P. brachiata was previously categorized under the genus Salsola in Salsoleae and has been a long-standing topic of debate. Therefore, the complete chloroplast genome of P. brachiata must be studied to provide a theoretical reference for species classification. In this study, we sequenced P. brachiata samples and determined the species' complete chloroplast genome. The complete chloroplast genome was 149,922 bp in length, with one large single copy (LSC: 83,565 bp), one small single copy (SSC: 18,535 bp), and two inverted repeat regions (IRa and IRb, 23,911 bp each). It contains 132 genes, including 87 protein-coding, eight rRNA, and 37 tRNA genes. The phylogenetic position showed that P. brachiata has the closest relationship with Caroxylon passerinum (accession number: NC057191.1). This study will provide genetic information and be beneficial to understanding the systematic position of P. brachiata within the Amaranthaceae. [ABSTRACT FROM AUTHOR]

Pennycress (Thlaspi arvense L.) is an annual plant in temperate regions that often grows as a weed. Pennycress is being domesticated as a new winter cover crop and oilseed crop for incorporation in the Midwest United States corn-soybean rotation, where it could offer economic and environmental benefits. While pennycress is gaining attention as a promising new crop, there remains a significant gap in understanding its interaction with insect communities and agroecosystems. This review compiles available information on insect herbivores (potential pests) and beneficial insects associated with pennycress growing in the wild (natural areas) or as a weed in agricultural areas. The limited knowledge on the response of pennycress to stressors (defoliation, stem injury and stand loss) similar to injury that could be caused by insects is also compiled here. By shedding light on the insects associated with pennycress and how pennycress might respond to injury from insect pests, this review sets the stage for further research and development of integrated pest management programs for insect pests of this new crop. [ABSTRACT FROM AUTHOR]

Plant biotechnology has become a viable strategy for agriculture, its applications include strategies aimed at obtaining and maintaining pathogen-free cultivars, genetic improvement (anther cultivation, embryo rescue, etc.) and micropropagation, used to obtain a greater number of individuals in less time and reduced spaces. Each of its phases depends largely on an adequate culture medium, since the growth, development and morphogenesis of the explant is mainly due to its chemical composition. For the production of garlic "seed" (Allium sativum L.), all phases are carried out in a growth chamber under automatically controlled aseptic and artificial conditions and at a temperature ranging between 22-25 °C. Somatic embryogenesis allows complete plants to be regenerated from a single cell. Garlic is an annual plant that only reproduces asexually, which is why bacteria, fungi and mainly viruses, which contribute to a 50-80 % decrease in its yield, affect it. An alternative to avoid these problems is the use of chemotherapy, thermotherapy or the combined use of one of these techniques with the cultivation of meristems, to obtain plants of high biological quality. Depending on the behavior of the culture during its in vitro stage, its response will be in the acclimatization phase and later in field conditions. This work was carried out with the objective of deepening the knowledge of some biotechnological techniques that are applied in the cultivation of garlic. [ABSTRACT FROM AUTHOR]

Although seedling has a significant role in the maintenance of plant diversity, productivity, and biochemical cycles in the rangeland. However, little is known about the influence of environmental factors in the seedling establishment, as well as the differences in the morphological development among species. To understand of seedlings establishment becomes of crucial importance to improve the success of reseeding of natural ecosystems. This literature review investigated which factors are addressed with failures in the seedling establishment of native grasses in rangeland conditions. Germinating seed grass is not big a problem if there are optimum environmental conditions. The heart of the matter is to ensure the survival and growth of these seedlings until the complete establishment as plant. The moisture and temperature of soil are the main environmental factors associated with failures in seedling establishment. The studies reviewed showed that annual plants have higher seedling growth rates, however lower allocation to reproductive structures when compared to mid-seral and late successional plants. These differences also promote different rates of seedling survival rate, with early seral grass showing higher rates than late seral. Apparently, the main cause of seedlings failures in the establishment is correlated with the development and extension of the adventitious roots. Where the reports describe that seedling emerge quickly and abundantly in most grasses, but the seedlings died between six and ten weeks of age. It was addressed that a plant can germinate and sprout the primary roots, however, for an unknown reason the plant does not sprout the adventitious root. [ABSTRACT FROM AUTHOR]

In Europe, the largest and most floristically diverse ultramafic (serpentine) outcrops are located on the Balkan Peninsula. In Greece, the Northern Pindus mountain range is characterized as a floristic diversity hotspot, due to the presence of large ultramafic outcrops that harbor a large number of nickel (Ni) hyperaccumulators with potential use in agromining. The work presented here is part of a more in‐depth study of floristic composition and ecology of plant communities in ultramafic grasslands of the Northern Pindus mountain range. In 2021–2022, 120 relevés in three subareas (sA1, sA2, and sA3) were sampled according to the Braun‐Blanquet method. We identified 493 plant taxa belonging to 61 families. The most abundant families were Asteraceae, Poaceae, Fabaceae, Lamiaceae, and Caryophyllaceae. A total of 22 plant taxa are endemic to Greece. Ninety‐five taxa are of Balkan origin while 158 taxa are distributed to the broader Mediterranean region. The highest number of plant life‐forms are hemicryptophytes (56.39%) and therophytes (23.33%). Eight Ni hyperaccumulators including Centaurea thracica, Bornmuellera baldaccii, Bornmuellera emarginata, Bornmuellera tymphaea, Noccaea boeotica, Noccaea tymphaea, Odontarrhena chalcidica, and Odontarrhena smolikana were recorded. [ABSTRACT FROM AUTHOR]

The annual or ephemeral plant Parthenium hysterophorus L. is an upright and heavily branched member of the Asteraceae family. The herb is used to treat heart issues, fever, anemia, wounds, and ulcerated sores. It is applied topically to treat skin issues, while the plant's decoction is frequently taken orally to address a wide range of ailments. Psoriasis, rheumatoid arthritis, diarrhea, urinary tract infections, dysentery, malaria, psoriasis, allergies, asthma, tinnitus, nausea, vomiting, and neuralgia are just a few of the health benefits that P. hysterophorus L. can provide. Asthma, bronchitis, dermatitis, and hay fever are some of the major problems caused by this weed, which is harmful to all living things because it also depletes nutrient levels and competes with the cultivation of major crops and vegetation. This review discusses the potential health benefits as well as the origin, distribution, morphology, and harmful effects of the plant on human health. [ABSTRACT FROM AUTHOR]

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Range expansions, whether they are biological invasions or climate change-mediated range shifts, may have profound ecological and evolutionary consequences for plant–soil interactions. Range-expanding plants encounter soil biota with which they have a limited coevolutionary history, especially when introduced to a new continent. Past studies have found mixed results on whether plants experience positive or negative soil feedback interactions in their novel range, and these effects often change over time. One important theoretical explanation is that plants locally adapt to the soil pathogens and mutualists in their novel range. We tested this hypothesis in Dittrichia graveolens , an annual plant that is both expanding its European native range, initially coinciding with climate warming, and rapidly invading California after human introduction. In parallel greenhouse experiments on both continents, we used plant genotypes and soils from 5 locations at the core and edge of each range to compare plant growth in soil inhabited by D. graveolens and nearby control microsites as a measure of plant–soil feedback. Plant–soil interactions were highly idiosyncratic across each range. On average, plant–soil feedbacks were more positive in the native range than in the exotic range. In line with the strongly heterogeneous pattern of soil responses along our biogeographic gradients, we found no evidence for evolutionary differentiation between plant genotypes from the core to the edge of either range. Our results suggest that the evolution of plant–soil interactions during range expansion may be more strongly driven by local evolutionary dynamics varying across the range than by large-scale biogeographic shifts. [ABSTRACT FROM AUTHOR]

Biennial vegetable crops are challenging to breed due to long breeding cycle times. At the same time, it is important to preserve a strong biennial growth habit, avoiding premature flowering that renders the crop unmarketable. Gene banks carry important genetic variation which may be essential to improve crop resilience, but these collections are underutilized due to lack of characterization for key traits like bolting tendency for biennial vegetable crops. Due to concerns about introducing undesirable traits such as premature flowering into elite germplasm, many accessions may not be considered for other key traits that benefit growers, leaving crops more vulnerable to pests, diseases, and abiotic stresses. In this study, we develop a method for characterizing flowering to identify accessions that are predominantly biennial, which could be incorporated into biennial breeding programs without substantially increasing the risk of annual growth habits. This should increase the use of these accessions if they are also sources of other important traits such as disease resistance. We developed the CarrotOmics flowering habit trait ontology and evaluated flowering habit in the largest (N=695), and most diverse collection of cultivated carrots studied to date. Over 80% of accessions were collected from the Eurasian supercontinent, which includes the primary and secondary centers of carrot diversity. We successfully identified untapped genetic diversity in biennial carrot germplasm (n=197 with 0% plants flowering) and predominantly-biennial germplasm (n=357 with <15% plants flowering). High broad-sense heritability for flowering habit (0.81 < H²< 0.93) indicates a strong genetic component of this trait, suggesting that these carrot accessions should be consistently biennial. Breeders can select biennial plants and eliminate annual plants froma predominantly biennial population. The establishment of the predominantly biennial subcategory nearly doubles the availability of germplasm with commercial potential and accounts for 54% of the germplasm collection we evaluated. This subcollection is a useful source of genetic diversity for breeders. This method could also be applied to other biennial vegetable genetic resources and to introduce higher levels of genetic diversity into commercial cultivars, to reduce crop genetic vulnerability. We encourage breeders and researchers of biennial crops to optimize this strategy for their particular crop. [ABSTRACT FROM AUTHOR]

The açaí (Euterpe oleracea Mart.) has an ethno-variety white characterized by the absence of anthocyanin in its epicarp, differing from violet açai. White ethno-variety is under strong anthropic pressure, which can lead to genetic erosion. Studies with white açaí that allow the selection of possible cultivars to stimulate their cultivation are scarce. Therefore, this work aimed to verify which characteristics best discriminate selected progenies of white açaí. The experiment contains 52 progenies installed, in a completely randomized design, with ten replications of one plant per plot in a lowland area belonging to Embrapa Amazônia Oriental. The characteristics evaluated were total bunch weight (PTC) in kilogram; fruit weight per bunch (PFC) in kilogram; fruit yield per bunch (RFC) in %; one hundred fruit weight (PCF) in grams; rachillas number per bunch (NRC); and rachilla length in the bunch (CRC), in centimeter. The conclusions are that there exists a correlation between the characteristics in different intensities through multivariate analysis; the Box Plot indicates that the plants respond to the annual water quantity specifically in the characteristics PTC, CRC, and PFC; for the ADC, the characteristics with the greatest contribution to the variance are PTC, CRC and PFC, being similar for the ACP mainly in the PTC and PFC characteristics; by the canonical Biplot the characteristics PTC, PFC, CRC and NRC are preferentially evaluated, discarding RFC and PCF, reducing the effort to be effective. [ABSTRACT FROM AUTHOR]

Quantifying the relative strength of isolating barriers is a major focus of research on plant speciation. Contrasting life histories and mating systems have the potential to limit gene exchange between closely related populations growing in sympatry. However, few studies have quantified reproductive isolating barriers between conspecific annual and perennial populations and their contributions to total reproductive isolation (RI). Incarvillea sinensis Lam. (Bignoniaceae) is an insect‐pollinated herb with largely allopatric annual and perennial populations that differ in mating systems. The perennial populations are primarily outcrossing whereas annual populations are predominantly selfing. At a rare sympatric site in northern China we estimated prezygotic and postzygotic barriers to gene exchange between annual and perennial plants and found complete RI between the two life histories. Annuals exhibited significantly higher ecogeographic isolation than perennials whereas perennials experienced more isolation through pollen–pistil interactions than annuals. Crosses between annuals and perennials demonstrated that postzygotic barriers influencing fruit and seed formation, F1 germination and survival were negligible for annuals but played a small role for perennials. However, F1 hybrids of crosses between annuals and perennials produced no pollen and their ovules were largely sterile. Our study provides insight into the relative importance of prezygotic and postzygotic isolating barriers between closely related annual and perennial populations of I. sinensis and some of these barriers could have been involved with speciation. Annuals and perennials of I. sinensis represent two biological species and thus deserve to be recognized as distinct taxonomic species. [ABSTRACT FROM AUTHOR]

Medicinal plants, as a good therapeutic strategy, can be used to treat a wide spectrum of diseases. Coriandrum sativum (C. sativum) is a plant from Apiaceae family. Numerous reports indicate that C. sativum has positive effects on anxiety, seizure, learning and memory ability, as well as pain. This annual plant can also treat colitis, increase appetite, reduce blood pressure, and attenuate myocardial damage. Additionally, it improves liver and kidney function and diabetes mellitus and inhibits osteoclast activity. These beneficial effects of C. sativum mainly are attributed to its antioxidant and antiinflammatory properties. The present paper reviews the impacts of C. sativum on different body systems. Information was extracted by searching the Web of Science, PubMed, Scopus, and Google Scholar from January 2010 until the end of February 2024. [ABSTRACT FROM AUTHOR]

Copyright of Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas is the property of Universidad de Santiago de Chile and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Flowering time is an important phenological trait in plants and a critical determinant of the success of pollination and fruit or seed development, with immense significance for agriculture as it directly affects crop yield and overall food production. Shifts in the growth season, changes in the growth season duration and changes in the production rate are environmental processes (potentially linked to climate change) that can lead to changes in flowering time in the long‐term due to selection. In contrast, biomass loss (due to, for example, herbivory or diseases) can have profound consequences for plant mass production and food security. We model the effects of these environmental processes on the flowering time evolutionarily stable strategy (ESS) of annual plants and the potential consequences for reproductive output. Our model recapitulates previous theoretical results linked to climate change and light competition and makes novel predictions about the effects of biomass loss on the evolution of flowering time. Our analysis elucidates how both the magnitude and direction of the evolutionary response can depend on whether biomass loss occurs during the earlier vegetative phase or during the later reproductive phase and on whether or not plants are adapted to grow in dense, competitive environments. Specifically, light competition generates an asymetric effect of mass loss on flowering time even when loss is indiscriminate (equal rates), with vegetative mass loss having a stronger effect on flowering time (resulting in greater ESS change) and final reproductive output. [ABSTRACT FROM AUTHOR]

Hemp, scientifically known as Cannabis sativa L., is an annual plant belonging to the Cannabaceae family. Its historical utilisation by people spans various purposes, including textiles and food. Considered one of humanity's earliest cultivated plants, hemp has played a significant role in various civilisations. Traditionally, its fibres have been employed in producing ropes, apparels and fabrics, while its seeds have been valued for their nutritional richness. Hemp's health benefits are also associated with phenolic compounds, which occur in varying amounts in almost all classes of plant foods and agroindustrial residues. Hemp seed (Cannabis sativa L.) cake, derived from the cold-pressing process of hemp seed oil extraction, is abundant in protein, fibre and biologically active compounds. Three types of hemp seeds (Silvana, Armanca, Teodora) grown at the Lovrin Agricultural Research and Development Station were subsequently studied after oil extraction. The recovered hemp seed leftover waste cakes were analysed to compare their physical, chemical, nutritional composition, and total phenolic content. The ash, moisture, lipid, total protein, and crude fibre levels were measured for each variety. The results will enable further research to valorise secondary byproducts generated during the hemp seed oil extraction and contribute to reducing food waste, thereby advancing the sustainability of the agri-food systems. [ABSTRACT FROM AUTHOR]

Plants display various forms of phenotypic plasticity in anticipation of changing conditions, many of which are influenced by information obtained from neighbouring plants. Here, we tested the hypothesis that cleistogamic Lamium amplexicaule plants can adaptively modify production of chasmogamous (CH) and cleistogamous (CL) flowers based on the perception of conspecific neighbours.The production and proportion of CH and CL flowers was examined in individual L. amplexicaule grown at varying densities or treated with root leachates from plants grown at different densities.When growing at high density or treated with root leachates from high‐density pots, L. amplexicaule increased production of more expensive, potentially outcrossing CH flowers. In contrast, single plants or plants treated with root leachates from empty pots or single‐source plants predominantly developed cheaper, self‐pollinated CL flowers.The results demonstrate a novel root‐based neighbour‐perception modality that enables plants to adaptively adjust production of CH and CL flowers in response to the presence of potential reproductive partners. Further research is needed to explore the broader ecological implications of this novel interplant cueing on reproductive bet‐hedging and plasticity in natural settings, as well as to identify the involved cues and their mode of operation. [ABSTRACT FROM AUTHOR]

Questions: Annual species have evolved sets of germination cues that are thought to be predictive of the post‐germination environment. In naturally patchy environments, germination microsites often vary considerably in the amount of light they receive and in the diurnal temperature fluctuations they experience. However, whether species' differential germination responses to light and temperature are associated with their spatial patterns of occurrence remains largely untested. Location: Mediterranean‐climate woodlands in Southwest Western Australia. Methods: We surveyed species' occurrences in annual plant communities in 150 quadrats across gradients of canopy cover and litter cover. Nineteen species recorded in this survey were then included in a germination experiment that manipulated (1) Light vs Dark (12 h light or continuous dark) approximating seeds near the soil surface vs those covered by litter and (2) Cold vs Warm temperature regimes (7/18°C and 7/24°C) approximating diurnal fluctuations experienced in shaded vs sun‐exposed microsites, respectively. Results: In the germination experiment, six species had highest germination probabilities in the Light treatment (regardless of temperature), five in Cold + Light, one in Warm + Light, two were indifferent to the treatments, and four did not germinate at all. Binomial linear mixed‐effects models showed that species' maximum responses to light and temperature did not explain their spatial distributions along canopy cover and litter cover gradients, contrary to theoretical expectations of germination being a strong driver of species' occurrences. Conclusions: Despite variation in species' responses to experimental treatments, no association was found with their field microsite associations. Germination strategies in our system were wider than expected for Mediterranean systems. Our results support that germination cues are not strong drivers of microhabitat associations in this system. [ABSTRACT FROM AUTHOR]

Zinnia peruviana (Asteraceae) is an annual flowering plant cultivated in various gardens of Mysore, the south of Karnataka state, India. During 2021, phytoplasma‐like symptoms such as virescence and phyllody were observed on Zinnia in Mysore local gardens. A total of 10 symptomatic and five asymptomatic samples were collected, and the DNA was amplified by PCR using primers specific to 16S RNA and secA genes. The nucleotide sequence analysis showed that the 16S RNA and SecA gene sequences were most similar to those of 'Ca. P. aurantifolia', with identities greater than 99%. The phytoplasma sequence from this study shared a common clade with 'Ca. P. aurantifolia' when phylogenetic analysis was done. Similarly, the reference sequences for the 16S rRNA and SecA region confirmed the identity of the phytoplasma associated with the leaves of Zinnia. This is the new host record for 'Ca. P. aurantifolia' (16SrII‐D) phytoplasma associated with Z. peruviana from India. [ABSTRACT FROM AUTHOR]

Type conversion from native shrubland to invasive annual grassland is on the rise due to global change factors such as prolonged drought and increasing fire frequency. Efforts to restore chaparral ecosystems are limited by current understanding of competitive interactions between shrub seedlings and invasive grasses as well as soil moisture requirements of chaparral seedlings. We set up a restoration experiment where we out‐planted Adenostoma fasciculatum seedlings, manipulated invasive grass density, monitored soil moisture at two depths, and tracked seedling survival and biomass. We found that higher invasive grass cover was associated with higher rates of seedling mortality but found no difference in biomass per surviving plant. Soil moisture was higher at 15 cm under the 100% weeded treatment than the 50% weeded and control treatments during January. Lower invasive cover resulted in higher richness of annual native plant species, as plots with 100% invasive removal had higher richness than 50% removal and unplanted control plots. Future restoration efforts in the chaparral will likely be more successful in increasing initial seedling establishment if invasive grass removal is included. [ABSTRACT FROM AUTHOR]

The total amount of sugarcane planting in China ranks third in the world, and Guangxi is the largest sugarcane planting base in China. In order to help the Guangxi government and industry better understand the annual planting distribution and scale of sugarcane in the whole region, it is convenient to optimize the planting structure and improve the efficiency of resource utilization, so as to ensure the stable development of Guangxi sugar industry. Based on the satellite-based hyperspectral image data of ' Zhuhai No.1 ', this paper carries out sugarcane recognition and area extraction, which fills the application gap of satellite-based hyperspectral image data in extracting sugarcane area in China. A globally optimized extended random walk model was used to identify and extract sugarcane by combining the spatial-spectral information of hyperspectral imagery. Firstly, the Initial probability estimation is performed using the LOR classifier, and then use the data after MNF dimensionality reduction to construct a weighted graph, and finally the probability optimization and recognition are carried out based on the extended random walk model. The results show that the spatial-spectral joint method based on extended random walk can effectively identify sugarcane, and the sugarcane recognition accuracy of 2021 year image can reach 91.4%, the misrecognition rate is 2.8%; the sugarcane recognition accuracy of 2020 year image can reach 90.5 %, the misrecognition rate is 3.1%. [ABSTRACT FROM AUTHOR]

Copyright of Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas is the property of Universidad de Santiago de Chile and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The "Annual Review of Plant Biology, 2023" is a comprehensive collection of articles that cover a wide range of topics in the field of plant biology. The book review highlights the importance of these topics in advancing our understanding of plant biology and their potential applications in fields such as synthetic biology and metabolic engineering. Some of the topics covered include plant gene regulation, small RNAs in plants, epigenetic modifications, chromatin remodeling, and photosynthesis. The review provides a valuable resource for researchers and students interested in plant biology and agriculture. [Extracted from the article]

Urban development greatly alters the natural and semi‐natural habitats of native plants. Urbanisation results in a range of diverse habitats including remnant agricultural lands, urban parks, and roadside habitats. This habitat diversity often promotes trait divergence within urban areas. However, the mechanisms by which diverse urban habitats influence the population genetic structure of individual plant species remain poorly understood. We investigated the effects of urbanisation on genetic diversity and structure within 24 Commelina communis populations across diverse habitat types (rural agricultural land, urban agricultural land, urban park land, and urban roadsides) within the Kyoto–Osaka–Kobe megacity in Japan. We conducted multiplexed inter‐simple sequence repeat genotyping to compare genetic diversity among populations in different habitats. We also examined the correlation between Nei's genetic distance and geographic and environmental distances and performed principal coordinate analysis (PCoA) to evaluate genetic differentiation among urban habitats. There were no significant differences in genetic diversity indices between urban and rural populations and among urban habitat types. Although we detected no isolation‐by‐distance structure in population pairs of the same habitat type and in those of different habitats, the difference in surrounding landscape facilitated genetic differentiation not only between urban and rural habitats but also between different urban habitats. PCoA revealed no clear genetic differentiation among rural and urban habitat populations. Our findings indicate that the establishment of diverse habitat types through urbanisation has no and little impact on genetic diversity and structure, respectively, in C. communis, likely due to its high selfing rate and ability to adapt to urban conditions. [ABSTRACT FROM AUTHOR]

Removal of non-native plants is known to increase overall native cover within degraded communities that contain at least a small percentage of native plant cover. We investigated the mechanisms behind this pattern, asking whether removal of non-native annual species increases the density and species richness of the native community through increased seedling recruitment or through the growth of established native shrubs. We also investigated whether the effectiveness of non-native removal was influenced by region (coastal versus inland) and whether there was a threshold of native cover required for invasive removal to be effective. We established 13 study sites (7 coastal and 6 inland) located throughout the Nature Reserve of Orange County, CA, USA. Each degraded site contained four paired plots corresponding to a range of existing native plant cover: low 20–29%, medium-low 30–39%, medium-high 40–49%, and high cover 50–59% with one plot per pair subjected to non-native removal. We collected plant density, species richness, and established native shrub volume measurements to clarify the effectiveness of non-native removal. Non-native plant removal reduced non-native annual recruitment, increased that of native shrub seedlings, but had no impact on native forb recruitment. Non-native removal increased the number and reduced mortality of established native shrubs but did not influence shrub size. Native seedling density, species richness, and established native shrub number were highest inland, but coastal sites had larger adult shrubs. We found that non-native removal was most effective for increasing native density and species richness for degraded inland sites with less than 40% of existing native cover. The initial native cover did not affect established shrub volume or number. Our results confirm the importance of non-native plant removal in areas with medium-low or low native cover to increase native recruitment, species richness, adult shrub number, and to reduce established shrub mortality, especially during extreme drought. [ABSTRACT FROM AUTHOR]

Ceratocarpus arenarius (Chenopodiaceae) is an under-investigated annual plant that occurs in dry areas stretching from eastern and south-eastern Europe to East Asia. This article presents the botanical characterization and examination of proximate parameters, minerals and cytotoxic activity of C. arenarius that grows wild in Kazakhstan. The results of morphological analysis using a light microscope, based on cross-sections of stems, roots and leaves, provide the necessary data to develop a regulatory document for this herbal substance as a raw material for use in the pharmaceutical, cosmetic and food industries. The investigated proximate characteristics included moisture content (6.8 ± 0.28%), ash (5.9 ± 0.40%), fat (12.5 ± 21.28%) and protein (392.85 ± 25.50). The plant is also rich in minerals (mg/100 g dry weight); Na (20.48 ± 0.29), K (302.73 ± 1.15), Zn (4.45 ± 0.35), Fe (1.18 ± 0.03), Cu (0.11 ± 0.02), Mn (0.76 ± 0.01), Ca (131.23 ± 0.09) and Mg (60.69 ± 0.72). The ethanolic extract of C. arenarius showed no acute toxicity against the brine shrimp nauplii. [ABSTRACT FROM AUTHOR]

Variations in plant characteristics in response to habitat heterogeneity can provide valuable insights into the mechanisms governing plant responses to environmental conditions. In this study, we investigated the role of environmental factors associated with arid conditions in shaping the phenotypic responses of an arid annual plant, Anastatica hierochuntica, across several populations found along an aridity gradient and across multiple spatial scales. Utilizing both field surveys and a net house experiment, we assessed the effects of environmental factors on trait variability within and between populations. The results indicated a significant convergence in plant height due to site aridity, reflecting growth potential based on abiotic resources. Convergence was also observed in the plant's electrolyte leakage with aridity and in plant height concerning soil salinity at specific sites. Phenotypic plasticity was pivotal in maintaining trait variability, with plant height plasticity increasing with soil salinity, SLA plasticity decreasing with aridity, and leaf number plasticity rising with aridity. In conclusion, our findings underscore the adaptive significance of phenotypic variability, especially plasticity, in arid conditions. Notably, trait variability and plasticity did not consistently diminish in stressful settings, emphasizing the adaptive value of flexible responses in such environments. [ABSTRACT FROM AUTHOR]

Adlay is an annual plant known for its abundant bioactive compounds and diverse pharmacological activities. Coixol, a key component found in various parts of adlay, significantly contributes to its biological activity. This study was conducted to extract adlay sprouts and seeds using different solvents (methanol and ethanol) and extraction methods (reflux and ultrasonic extraction). The extracts were then evaluated for their total polyphenol and flavonoid contents, as well as antioxidant ability (DPPH and ABTS+). Additionally, the coixol content of these extracts was analyzed using HPLC/DAD analysis. The results showed that the extraction methods and solvents used impacted the bioactive compounds and their activities in the samples. Adlay sprouts exhibited a higher compound content and stronger antioxidant capacity than adlay seeds. Moreover, a substantial amount of coixol was found in the sprouts, while it was not detected in the seeds. This study emphasizes the importance of selecting appropriate extraction methods to optimize the biological activities of adlay sprouts and seeds. Adlay sprouts, with their enriched phytochemical compounds and enhanced antioxidant ability, could serve as a valuable material for health product applications. [ABSTRACT FROM AUTHOR]

Background: Investigations that are focused on arbuscular mycorrhizal fungus (AMF) biodiversity is still limited. The analysis of the AMF taxa in the North Caucasus, a temperate biodiversity hotspot, used to be limited to the genus level. This study aimed to define the AMF biodiversity at the species level in the North Caucasus biotopes. Methods: The molecular genetic identification of fungi was carried out with ITS1 and ITS2 regions as barcodes via sequencing using Illumina MiSeq, the analysis of phylogenetic trees for individual genera, and searches for operational taxonomic units (OTUs) with identification at the species level. Sequences from MaarjAM and NCBI GenBank were used as references. Results: We analyzed >10 million reads in soil samples for three biotopes to estimate fungal biodiversity. Briefly, 50 AMF species belonging to 20 genera were registered. The total number of the AM fungus OTUs for the "Subalpine Meadow" biotope was 171/131, that for "Forest" was 117/60, and that for "River Valley" was 296/221 based on ITS1/ITS2 data. The total number of the AM fungus species (except for virtual taxa) for the "Subalpine Meadow" biotope was 24/19, that for "Forest" was 22/13, and that for "River Valley" was 28/24 based on ITS1/ITS2 data. Greater AMF diversity, as well as number of OTUs and species, in comparison with that of forest biotopes, characterized valley biotopes (disturbed ecosystems; grasslands). The correlation coefficient between "Percentage of annual plants" and "Glomeromycota total reads" r = 0.76 and 0.81 for ITS1 and ITS2, respectively, and the correlation coefficient between "Percentage of annual plants" and "OTUs number (for total species)" was r = 0.67 and 0.77 for ITS1 and ITS2, respectively. Conclusion: High AMF biodiversity for the river valley can be associated with a higher percentage of annual plants in these biotopes and the active development of restorative successional processes. [ABSTRACT FROM AUTHOR]

Purpose: Perennial crops have been suggested as a more sustainable alternative to the currently most common cropping systems. Compared with annual plants, perennial plants produce more biomass and have deeper roots, and are expected to lead to higher soil organic carbon (SOC). This hypothesis, however, has not been well tested for grain crops. Methods: Using perennial intermediate wheatgrass (IWG, Thinopyrum intermedium) and annual winter wheat (Triticum aestivum) as focal species, and native grassland as reference, we quantified the SOC accumulation via a process-based model, describing water and heat exchanges and carbon-nitrogen cycling in the canopy and soil to a depth of 2 m. The model includes C fixation via photosynthesis, plant biomass growth and litter production, physical protection of SOC, depolymerisation, C mineralisation, nitrification, denitrification, microbial growth, and necromass turnover in the soil. While of general applicability, we considered a sandy loam under warm-summer humid continental climate. Results: Following a conversion from native grassland, IWG reduced SOC losses by at least 38%, especially in the particulate organic carbon (POC) pool, within the top 2 m of soil, compared with annual wheat. Soil microbial biomass and soil respiration were higher in IWG than annual wheat. Shifting from annual wheat to high photosynthetic capacity IWG increased SOC by about 33 g C m−2 y−1 (averaged over a 4-year continuous IWG cropping), with a large fraction of SOC gain stemming from restoring POC. Conclusion: Compared with annual grains, perennial grains can increase soil carbon sequestration and maintain SOC at levels nearer to that of native grasslands. [ABSTRACT FROM AUTHOR]

Xanthomonas spp. infect a wide range of annual and perennial plants. Bacterial blight in young seedlings of Eucalyptus spp. in Indonesia was originally identified as X. perforons. However, these strains failed to elicit a hypersensitive response (HR) on either tomatoes or peppers. Two of the strains, EPK43 and BCC 972, when infiltrated into tomato and pepper leaves, failed to grow to significant levels in comparison with well-characterized X. euvesicatoria pv. perforons (Xp) strains. Furthermore, spray inoculation of 'Bonny Best' tomato plants with abacterial suspension of the Eucalyptus strains resulted in no obvious symptoms. We sequenced the whole genomes of eight strains isolated from two Eucalyptus species between 2007 and 2015. The strains had average nucleotide identities (ANIs) of at least 97.8 with Xp and X. euvesicatoria pv. euvesicatoria (Xeu) strains, both of which are causal agents of bacterial spot of tomatoes and peppers. A comparison of the Eucalyptus strains revealed that the ANI values were >99.99% with each other. Core genome phylogeny clustered all Eucalyptus strains with X. euvesicatoria pv. rosa. They formed separate clades, which included X. euvesicatoria pv. alangii, X. euvesicatoria pv. citrumelonis, and X. euvesicatoria pv. alfalfae. Based on ANI, phylogenetic relationships, and pathogenicity, we designated these Eucalyptus strains as X. euvesicatoria pv. eucalypti (Xee). Comparative analysis of sequenced strains provided unique profiles of type III secretion effectors. Core effector XopD, present in all pathogenic Xp and Xeu strains, was absent in theXee strains. Comparison of the hrp clusters of Xee, Xp, and Xeu genomes revealed that HrpE in Xee strains was very different from that in Xp and Xeu. To determine if it was functional, we deleted the gene and complemented with the Xee hrpE, confirming it was essential for secretion of type III effectors. HrpE has a hypervariable N-terminus in Xanthomonas spp., in which the N-terminus of Xee strains differs significantly from those of Xeu and Xp strains. [ABSTRACT FROM AUTHOR]