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Trait-based approaches that complement taxonomy-based studies have increased in popularity among the scientific community over the last decades. The collection of biological and ecological characteristics of species (i.e., traits) provides insight into species and ecosystem vulnerability to environmental and anthropogenic changes, as well as ecosystem functioning. Here, we present the FUN Azores trait database, describe our approach, evaluate its scope, compare it to other marine trait databases, and explore the spatial distribution of its traits with "functional maps." While most of the available trait databases to date contain essential information to understand the functional diversity of a taxonomic or functional group, our ecosystem-based approach provides a comprehensive assessment of diverse fauna (i.e., meio-, macro-, and megafauna) from benthic and pelagic environments in the Azores Marine Park; including ridges, seamounts, hydrothermal vents, and the overlying water column. We used a collaborative approach involving 30 researchers with different expertise to develop the FUN Azores database, which contains compiled data on 14 traits representing morphological, behavioral, and life history characteristics for 1,210 species across 10 phyla. The "functional maps" show a distinct distribution of the two most common size classes, suggesting different communities with different functionalities. The following traits had the best scoring coverage (i.e., >95% of the species scored): maximum body size, body form, skeleton material, feeding structure, motility, environmental position, substratum affinity, distribution, and depth range; while traits related to species behavior (e.g., sociability or aggregation tendencies) and life history (e.g., developmental mechanism) had lower scoring coverage, highlighting the need for further research to fill these knowledge gaps. We found a larger number of species in the benthic compared to the pelagic environment and differing species composition between areas within the Azores Marine Park resulting from varying biodiversity, ecosystem types, sampling effort, and methodologies used. The FUN Azores database will foster and facilitate trait-based approaches in the area, develop a framework for expansion of crossecosystem and cross-taxa trait databases elsewhere, and improve our ecological understanding of the Azores Marine Park and its conservation requirements. [ABSTRACT FROM AUTHOR]

The Homeotic complex (Hox) genes play a crucial role in determining segment identity and appendage morphology in bilaterian animals along the antero‐posterior axis. Recent studies have expanded to agricultural pests such as fall armyworm (FAW), scientifically known as Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae), which significantly threatens global agricultural productivity. However, the specific role of the hox gene Sfabd‐B in FAW remains unexplored. This research investigates the spatial and temporal expression patterns of Sfabd‐B in various tissues at different developmental stages using quantitative real‐time polymerase chain reaction (qRT‐PCR). Additionally, we explored the potential function of the Sfabd‐B gene located in the FAW genome using CRISPR/Cas9 technology. The larval mutant phenotypes can be classified into three subgroups as compared with wild‐type individuals, that is, an excess of pedis in the posterior abdomen, deficient pedis due to segmental fusion and deviations in the posterior abdominal segments. Importantly, significant differences in mutant phenotypes between male and female individuals were also evident during the pupal and adult phases. Notably, both the decapentaplegic (dpp) and cuticular protein 12 (cp 12) genes displayed a substantial marked decrease in expression levels in the copulatory organ of male mutants and the ovipositor of female mutants compared with the wild type. These findings highlight the importance of Sfabd‐B in genital tract patterning, providing a potential target for improving genetic control. [ABSTRACT FROM AUTHOR]

Animal morphology is influenced by several factors, including gonadal development and gametogenesis. Although their effects are well documented in male/female differentiation, much less is known about same-sex effects, such as those caused by their mode of reproduction. Here, using geometric morphometric analyses, we compare two groups of all-female triploid hybrid fish Chrosomus eos × eos-neogaeus, that differ only by their sexual and asexual reproductive strategies. We demonstrate that morphological differences arise from factors inherently associated with their mode of reproduction, with results replicated in two distinct lineages and in natural and common garden environments. Such differences provide additional insight about the costs and benefits of both reproductive strategies, which have mostly been of a demographic, population genetic, or genetic nature. In particular, these findings have important implications for the ecology of asexual organisms and contribute to the study of sex evolution by adding complexity to the paradox of sex theory. Morphology associated with reproductive strategy in animals can be difficult to isolate. Here, the authors use geometric morphometrics to study triploid hybrid Chrosomus fish that reproduce sexually and asexually, identifying morphological differences associated with reproductive strategy. [ABSTRACT FROM AUTHOR]

The family Eirenidae is one of the major taxa of the order Leptothecata, comprising approximately 80 species from ten genera. In this study, taxonomic investigations, including morphological observations and molecular 16S phylogenetic analyses, were conducted on unknown Eirenidae specimens collected off the coast of Iwaki, Fukushima Prefecture, eastern Japan, in June 2022. The specimens had the following morphological characteristics: marginal warts and tentacular bulbs with lateral cirri and without adaxial papillae, a mouth with simple lips, four simple radial canals, and eight statocysts common to the genus Eutima. However, this species can be distinguished from other species of Eutima by the number of tentacles, number and shape of marginal warts, position of the gonads, and gastric peduncle length. Moreover, the monophyly of the species was evident in the 16S rRNA phylogenetic tree (as indicated by the high bootstrap value of 100%), thereby supporting the validity of the new species. Based on these results, we describe it as a new species, Eutima onahamaensis, for taxonomic stabilization. We also made detailed observations of the morphology and molecular phylogenetic analyses of one of the species newly recorded in Japan: Eutima diademata. A comparative table of the primary diagnostic characteristics of Eutima has been provided. This study provided taxonomic keys for identifying species in the genus Eutima. [ABSTRACT FROM AUTHOR]

Predation is a fundamental selective pressure on animal morphology, as morphology is directly linked with physical performance and evasion. Bipedal heteromyid rodents, which are characterized by unique morphological traits such as enlarged hindlimbs, appear to be more successful than sympatric quadrupedal rodents at escaping predators such as snakes and owls, but no studies have directly compared the escape performance of bipedal and quadrupedal rodents. We used simulated predator attacks to compare the evasive jumping ability of bipedal kangaroo rats (Dipodomys) to that of three quadrupedal rodent groups—pocket mice (Chaetodipus), woodrats (Neotoma), and ground squirrels (Otospermophilus). Jumping performance of pocket mice was remarkably similar to that of kangaroo rats, which may be driven by their shared anatomical features (such as enlarged hindlimb muscles) and facilitated by their relatively small body size. Woodrats and ground squirrels, in contrast, almost never jumped as a startle response, and they took longer to perform evasive escape maneuvers than the heteromyid species (kangaroo rats and pocket mice). Among the heteromyids, take‐off velocity was the only jump performance metric that differed significantly between species. These results support the idea that bipedal body plans facilitate vertical leaping in larger‐bodied rodents as a means of predator escape and that vertical leaping likely translates to better evasion success. [ABSTRACT FROM AUTHOR]

Despite being considered a paraphyletic group, the invertebrates are the most diverse group in the Animalia kingdom. The study of the morphology of these animals is fundamental in knowledge of ecology, habits, taxonomy and in applied research. Most commonly, conservation of invertebrates is done with a solution of 75–95% ethanol or 4–10% formalin. Plastination, a modern and innovative technique, promotes the replacement of tissue water with a curable polymer and produces non-toxic, dry, more resistant and easy-to-maintain specimens. However, the application of the technique to marine invertebrates for tissue conservation was not found in the literature. The objective of this work was to develop a plastination protocol for marine invertebrates, seeking to maintain the most natural appearance possible. In the study, 94 marine invertebrates from different phyla were used and the protocol was followed: anesthesia with magnesium chloride, fixation with 5% formalin, dehydration in pure acetone, impregnation with silicone at room temperature and application of the catalyst to curing the polymer. In general, after plastination, the 94 processed invertebrates were more resistant, durable, easier to handle and maintain, and without toxicity. No major changes in shape, size, or coloration were evident in the animals, except for porifera that lost their pigments and cnidarians that lost part of their volumes. The research results were excellent, with the production of high-quality material, enabling a wide range of applicability of the specimens, whether in the areas of education, research, or exposition. [ABSTRACT FROM AUTHOR]

Background: The diaphragm, the main muscle involved in respiration and one of those responsible for maintaining life, is still little explored in terms of its morphology in wild animals. There are few studies on the anatomy of Eira barbara, a carnivorous mustelid that is a victim of the urbanization process. In order to contribute to the conservation of the species, we described the topography and morphology of the diaphragm, which may be involved in injuries caused by the impacts of human activities. Results: We studied five specimens of Eira barbara, whose diaphragmatic muscle had a dorsal insertion on the 14th thoracic vertebra, laterally between the 8th and 13th intercostal space (EIC) and ventrally on the 8th EIC, with attachment to the xiphoid process. Consisting of three muscle regions (lumbar, costal and sternal), the diaphragm in Eira barbara showed radially arranged bundles, with the right costal muscle being slimmer than the left; the left pillar wider than the right and between them were the aortic and esophageal hiatuses. The Y-shaped tendinous center housed the foramen of the vena cava bordering the right costal region. In the most dorsal portion of the diaphragm, between the costal regions and the diaphragmatic pillar, we found two triangular-shaped regions devoid of muscle. Conclusions: Our findings, when compared with the current literature, indicate that the location and positioning of the diaphragm are independent of the physical conformation of the species, and that the right costal region, as well as the triangular areas devoid of musculature, may be fragile points for herniation in cases of Eira barbara being run over. [ABSTRACT FROM AUTHOR]

Dimensionless numbers have long been used in comparative biomechanics to quantify competing scaling relationships and connect morphology to animal performance. While common in aerodynamics, few relate the biomechanics of the organism to the forces produced on the environment during flight. We discuss the Weis-Fogh number, N , as a dimensionless number specific to flapping flight, which describes the resonant properties of an insect and resulting tradeoffs between energetics and control. Originally defined by Torkel Weis-Fogh in his seminal 1973 paper, N measures the ratio of peak inertial to aerodynamic torque generated by an insect over a wingbeat. In this perspectives piece, we define N for comparative biologists and describe its interpretations as a ratio of torques and as the width of an insect's resonance curve. We then discuss the range of N realized by insects and explain the fundamental tradeoffs between an insect's aerodynamic efficiency, stability, and responsiveness that arise as a consequence of variation in N , both across and within species. N is therefore an especially useful quantity for comparative approaches to the role of mechanics and aerodynamics in insect flight. [ABSTRACT FROM AUTHOR]

Tilorone dihydrochloride (tilorone) is an orally active interferon inducer with anticancer effects. The present study aimed to evaluate the anticancer effects of tilorone in breast cancer. MTT assay was done to measure the proliferation of MCF‐7 and MDA‐MB‐231 breast cancer cells after treatment with tilorone. Mammary carcinogenesis was induced by subcutaneous injection (35 mg/kg, 0.5 mL) of dimethylbenz[a]anthracene (DMBA) in mammary pads of Sprague Dawley (SD) rats. Tumors were allowed to grow for 16 weeks till their sizes reached to 550–700 mm3, and then treated with 10 and 20 mg/kg of tilorone and standard drug doxorubicin (4 mg/kg) twice a week for 3 weeks. Normal and disease‐control animals received normal saline. Tumor volumes and body weights were measured. Tumors were isolated to measure the levels of interferon‐β (IFN‐β), vascular endothelial growth factor‐A (VEGF‐A), P53 and inflammatory markers by enzyme‐linked immunosorbent assay (ELISA). Serum biochemistry, lipid peroxidation (LPO) and antioxidant enzymes were measured by standard methods. Histopathology and immunohistochemistry (IHC) of P53 was done in tumor sections. Tilorone reduced the proliferation of MCF‐7 and MDA‐MB‐231 cells with IC50 concentrations at 34.08 µM and 14.27 µM, respectively. Tilorone treatment showed reduced tumor volume, and increased survival with no significant changes in the body weights. Tilorone treatment also decreased levels of inflammatory markers and VEGF‐A and increased IFN‐β and P53 levels. Further, treatment with tilorone also decreased LPO and increased antioxidants levels. Histopathology of tumor sections showed normalizing morphology of treated animals. IHC of tumor sections showed increased levels of P53. In conclusion, tilorone has potential anticancer effects against breast cancer. [ABSTRACT FROM AUTHOR]

Simple Summary: The Sanmartinero creole bovine (SM) is found in the department of Meta (Colombian Orinoquia) and derives from the bullfighting cattle introduced by the Spanish in the 15th century. Its adaptation process has allowed it to develop adaptive characteristics to live in extreme climatic environments. Although there are studies on its reproductive and productive characteristics, little is known about possible asymmetries in some biological structures. Fluctuating asymmetry allows us to detect a possible developmental instability that may be caused by stressful conditions. The aim of our study was to present the current state of developmental instability in two bilateral cephalic characters of the SM creole bovine. The results showed a fluctuating asymmetry biased to the left for ear and horn length, and to the right for ear width and horn perimeter. We consider that the environment in which it lives may have an impact on the development of cephalic structures, creating stress in the animal, which causes the detected asymmetry. However, the authors are not aware of any studies on this topic. Asymmetric studies can indicate disturbances in the developmental process. Fluctuating asymmetry (FA) is considered an indicator of stress. The Sanmartinero (SM) creole bovine is native to the department of Meta (Colombian Orinoquia) and its adaptation process has allowed it to live in extreme tropical environments. The aim of this cross-sectional and descriptive study was to present the current state of the knowledge of asymmetries in some cephalic characters of the SM creole bovine. A total of 94 animals were studied (18 uncastrated males and 76 females) from three different farms, with an age range of 0.5–10 years. For each animal, two measurements of the ear (width and length) and two measurements of the horn (perimeter and length) were obtained in vivo. The degree of asymmetry was calculated as (R − L)/(R + L). Bilateral differences pointed towards a fluctuating asymmetry (e.g., a random variation in the trait that is expected to be perfectly symmetrical) biased towards right for ear width and horn perimeter, and towards left for ear and horn length. Since the development of these structures—ears and horns—is under the control of the same set of genes, the fluctuating asymmetry could constitute a reflection of a normal condition. [ABSTRACT FROM AUTHOR]

Simple Summary: In this study, we used both morphology and molecular genetic tools, namely mitochondrial DNA (mtDNA) control region and nuclear DNA (nDNA) exon involving recombination activating gene 2 (RAG2), to confirm the species of two sea turtles found in the Thai Andaman Sea that possessed unusual lateral scute patterns from the original species. Both turtles were identified as the olive ridley turtle (Lepidochelys olivacea). This finding has raised awareness about the need for exploration of rare phenotypes of carapace scute patterns in the olive ridley turtle populations that reside in Thai Seas and the Indo-Pacific region. Two stranded Lepidochelys-like sea turtles were rescued from the Thai Andaman Sea coastline by veterinarians of the Phuket Marine Biological Center (PMBC), one in May of 2019 and another in July of 2021. They were first identified as olive ridley turtles (Lepidochelys olivacea), as the external appearance of both turtles was closer to that species than the other four species found in the Thai Andaman Sea. In fact, when carefully examined, an unusual pattern of the lateral scutes on each turtle was observed, specifically symmetric 5/5 and asymmetric 5/6, both of which are considered rare for L. olivacea and had never been reported in the Thai Andaman Sea. In contrast, this characteristic was more common for the closely related species, Kemp's ridley (L. kempii), although this species is not distributed in the Indo-Pacific Ocean. Thus, we further investigated their genetic information to confirm species identification using two molecular markers, namely the mtDNA control region and nDNA RAG2. The results from the mtDNA control region sequences using the Basic Local Alignment Search Tool (BLAST) indicated that both individuals exhibited a higher percent identity with L. olivacea (99.81–100.00%) rather than L. kempii (94.29–95.41%) or any other species. A phylogenetic tree confirmed that these two turtles belonged to the L. olivacea clade. Moreover, the results of RAG2 also supported the mtDNA result, as both individuals shared the same RAG2 haplotype with L. olivacea. Thus, we have concluded that the two turtles with unusual lateral scute patterns exhibited genetic consistency with their original species, L. olivacea, which has brought attention to the importance of exploring rare phenotypes in sea turtle populations residing in Thai Seas. [ABSTRACT FROM AUTHOR]

The vast array of dog breeds showcases a remarkable diversity that extends to osteological differences. Exploring these morphological distinctions and establishing reference data for various dog breeds are essential steps in comprehending the evolutionary changes that dogs have undergone. In this study, we conducted linear measurements of the calcaneus and performed shape analyses on selected dog breeds to elucidate distinctive characteristics among them. X‐ray images of the calcaneus from six different dog breeds—Maltese Terrier, Toy Poodle, Pomeranian, Cavalier King Charles Spaniel, French Bulldog and Golden Retriever—were utilized for this investigation. Radiological images were obtained from a medio‐lateral exposure, positioned 30 cm away from the x‐ray device. From these images, four linear length measurements and two angle values were extracted. Additionally, a 2D geometric morphometric analysis was conducted using 32 semi landmarks placed on the radiological images. Linear measurements were assessed using ANOVA, while principal component analysis was employed to examine shape variations across all individuals. Shape differences between species were further elucidated through canonical variates analysis. The results revealed that the Golden Retriever exhibited the highest values for linear measurements, while the Pomeranian showed the lowest. Maltese Terriers displayed the highest dorsal calcaneal angle value. Notably, there were significant differences in calcaneal body length among all breeds, except for the Toy Poodle and Cavalier King Charles Spaniel. Moreover, Maltese Terriers exhibited statistically distinct angular measurements compared to other breeds. Principal component analysis unveiled that the first principal component explained 32.69% of the total variation, with the cranial edge of the calcaneal body being closer to the body in individuals with higher values. Shape variations also indicated that Golden Retrievers displayed a broader range of shapes compared to French Bulldogs, which exhibited a more conservative distribution. While there was no clear breed‐specific distinction according to the first principal component, Cavalier King Charles Spaniels generally showed lower values. In canonical variates analysis, distinctions in calcaneal shape between species were apparent, with Golden Retrievers, Cavalier King Charles Spaniels and French Bulldogs displaying positive values for the first canonical variates. The highest Procrustes distance was observed between Maltese Terriers and Cavalier King Charles Spaniels. Notably, allometry was found to be statistically insignificant. This comprehensive study utilized both linear and geometric morphometric analyses based on x‐ray images, yielding promising results. The integration of imaging systems in veterinary anatomy research presents numerous opportunities for studying animal welfare and health, utilizing various materials such as bones and cadavers. These advancements hold the potential for further enhancing our understanding of animal morphology and well‐being. [ABSTRACT FROM AUTHOR]

It is well known that maternal age at reproduction affects offspring lifespan and some other fitness-related traits, but it remains understudied whether maternal senescence affects how offspring respond to their environments. Early environment often plays a significant role in the development of an animal's behavioral phenotype. For example, complex environments can promote changes in cognitive ability and brain morphology in young animals. Here, we study whether and how maternal effect senescence influences offspring plasticity in cognition, group behavior, and brain morphology in response to environmental complexity. For this, juvenile 3-spined sticklebacks from young and old mothers (i.e. 1-yr and 2-yr-old) were exposed to different levels of environmental enrichment and complexity (i.e. none, simple, and complex), and their behavior, cognitive ability, and brain size were measured. Exposing fish to enriched conditions improved individual learning ability assessed by a repeated detour-reaching task, increased the size of the whole brain, and decreased aggressive interactions in the shoal. Maternal age did not influence the inhibitory control, learning ability, and group behavioral responses of offspring to the experimental environmental change. However, maternal age affected how some brain regions of offspring changed in response to environmental complexity. In offspring from old mothers, those exposed to the complex environment had larger telencephalons and cerebellums than those who experienced simpler environments. Our results suggest that maternal effect senescence may influence how offspring invest in brain functions related to cognition in response to environmental complexity. [ABSTRACT FROM AUTHOR]

Wool plays an irreplaceable role in the lives of livestock and the textile industry. The variety of hair quality and shape leads to the diversity of its functions and applications, and the finer wool has a higher economic value. In this study, 10 coarse and 10 fine ordos fine wool sheep skin samples were collected for RNAseq, and coarse and fine skin/hair follicle RNA-seq datasets of other five animal breeds were obtained from NCBI. Weighted gene co-expression network analysis showed that the common genes were clustered into eight modules. Similar gene expression patterns in sheep and rabbits with the same wool types, different gene expression patterns in animal species with different hair types, and brown modules were significantly correlated with species and breeds. GO and KEGG enrichment analyses showed that, most genes in the brown module associated with hair follicle development. Hence, gene expression patterns in skin tissues may determine hair morphology in animal. The analysis of differentially expressed genes revealed that 32 highly expressed candidate genes associated with the wool fineness of Ordos fine wool sheep. Among them, KAZALD1 (grey module), MYOC (brown module), C1QTNF6 (brown module), FOS (tan module), ITGAM, MX2, MX1, and IFI6 genes have been reported to be involved in the regulation of the hair follicle cycle or hair loss. Additionally, 12 genes, including KAZALD1, MYOC, C1QTNF6, and FOS, are differentially expressed across various animal breeds and species. The above results suggest that different sheep breeds share a similar molecular regulatory basis of wool fineness. Finally, the study provides a theoretical reference for molecular breeding of sheep breeds as well as for the investigation of the origin and evolution of animal hair. [ABSTRACT FROM AUTHOR]

Simple Summary: Stick insects and their mechanoreceptors are important models for neurobiology and physiology. Mechanoreceptors are sensory systems that detect mechanical stimuli like wind, airborne sound, substrate vibration, or gravity. They are present in insects in highly diverse forms. Here, the morphology of a mechanosensory complex (the subgenual organ complex) with two distinct organs in the legs of a stick insect (Sipyloidea chlorotica) is described for animals that were newly hatched. The overall organisation of this sensory complex is unique in stick insects, and the present study extends the current knowledge from adult insects to the early juvenile instars. The morphological results show a connection between the two sensory organs (subgenual organ and distal organ) by a thin membrane, which had already been documented in adult stick insects. By the time of hatching, both the sensory neurons activated by mechanical stimuli as well as further tissues in the sensory complex are present, and they likely develop during embryogenesis. The sensory organs could also be functional at hatching. This report discusses the implications of a connection between the subgenual organ and the distal organ, suggesting that the membrane would influence the displacements of the two organs. The subgenual organ complex of stick insects has a unique neuroanatomical organisation with two elaborate chordotonal organs, the subgenual organ and the distal organ. These organs are present in all leg pairs and are already developed in newly hatched stick insects. The present study analyses for the first time the morphology of sensory organs in the subgenual organ complex for a membrane connecting the two sensory organs in newly hatched insects (Sipyloidea chlorotica (Audinet-Serville 1838)). The stick insect legs were analysed following hatching by axonal tracing and light microscopy. The subgenual organ complex in first juvenile instars shows the sensory organs and a thin membrane connecting the sensory organs resembling the morphology of adult animals. Rarely was this membrane not detected, where it is assumed as not developed during embryogenesis. The connection appears to influence the shape of the subgenual organ, with one end extending towards the distal organ as under tension. These findings are discussed for the following functional implications: (1) the physiological responses of the subgenual organ complex to mechanical stimuli after hatching, (2) the influence of the membrane on the displacement of the sensory organs, and (3) the connection between the subgenual organ and distal organ as a possible functional coupling. [ABSTRACT FROM AUTHOR]

Understanding the ontogenetic age and sex composition of zooarchaeological assemblages can reveal details about past human hunting and herding strategies as well as past animal morphology and behavior. As such, the accuracy of our estimates underlies our ability to ascertain details about site formation and gain insights into how people interacted with different animals in the past. Unfortunately, our estimates typically rely on only a small number of bones, limiting our ability to fruitfully use these estimates to make meaningful comparisons to theoretical expectations or even between multiple assemblages. This paper describes a method to use zooarchaeological remains with standard biometric measurements to estimate the ontogenetic age and sex composition of the assemblage, focused on immature, adult-sized female, and adult-sized male specimens. The model uses a Bayesian framework to ensure that the parameter estimates are biologically meaningful. Simulated assemblages show that the model can accurately estimate the biometry and composition of zooarchaeological assemblages. Two archaeological case studies also show how the model can be applied to produce tangible insights. The first, focused on sheep from Neolithic Pinarbaşı B, highlights the model's ability to elucidate site formation and function. The second, focused on cattle remains from four assemblages from 7th-6th millennium BCE northwestern Anatolia, showcases how to use the mixture modeling results to compare assemblages to one another and to specific hypotheses. This modeling framework provides a new avenue for investigating long-term trajectories in animal biometry alongside contextual analyses of past human choices in butchery and consumption. [ABSTRACT FROM AUTHOR]

Animal camouflage serves a dual purpose in that it enhances both predation efficiency and anti‐predation strategies, such as background matching, disruptive coloration, countershading, and masquerade, for predators and prey, respectively. Although body size and shape determine the appearance of animals, potentially affecting their camouflage effectiveness, research over the past two centuries has primarily focused on animal coloration. Over the past two decades, attention has gradually shifted to the impact of body size and shape on camouflage. In this review, we discuss the impact of animal body size and shape on camouflage and identify research issues and challenges. A negative correlation between background matching effectiveness and an animal's body size has been reported, whereas flatter body shapes enhance background matching. The effectiveness of disruptive coloration is also negatively correlated with body size, whereas irregular body shapes physically disrupt the body outline, reducing the visibility of true edges and making it challenging for predators to identify prey. Countershading is most likely in larger mammals with smaller individuals, whereas body size is unrelated to countershading in small‐bodied taxa. Body shape influences a body reflectance, affecting the form of countershading coloration exhibited by animals. Animals employing masquerade achieve camouflage by resembling inanimate objects in their habitats in terms of body size and shape. Empirical and theoretical research has found that body size affects camouflage strategies by determining key aspects of an animal's appearance and predation risk and that body shape plays a role in the form and effectiveness of camouflage coloration. However, the mechanisms underlying these adaptations remain elusive, and a relative dearth of research on other camouflage strategies. We underscore the necessity for additional research to investigate the interplay between animal morphology and camouflage strategies and their coevolutionary development, and we recommend directions for future research. [ABSTRACT FROM AUTHOR]

The application of hay feeding devices, such as the use of hay nets or slow feeders, can help with the management of weight in ponies; however, there is still a lack of knowledge regarding their effect on equine posture. Therefore, the aim of the study was to assess the effect of different feeding devices on the posture of ponies using morphometric analysis. Two different breed types, Shetland type (SH, n = 5) versus Welsh Cob type (WC, n = 4), were fed the same forage in four different ways: on the ground (G), using a fully filled haynet (HF), using a partially filled haynet (HL) and using a slow‐feeder hay box (HB). Video recordings were obtained and then geometric morphometric analysis was applied. Breed morphology was confirmed by body morphometric measurements. Data were analysed statistically using one‐way ANOVA, canonical variate analysis (CVA), principal component analysis (PCA), partial least‐squares (PLS) analysis and multivariate analysis of variance (MANOVA). Moreover, a mixed model was performed to study differences in mandibular angle. SH and WC ponies were shown to have significantly different body morphometric measurements. The geometric morphometric analysis results showed that ponies arch their back and modify their neck shape differently according to the feeding method and their morphological group. For the neck, the SH and WC ponies adapted similarly to the use of small‐holed hay nets, but their posture varied when feeding from the ground or hay box. The back postures consistently differed according to the breed type and feeding method. The mandibular angle for both breed types was reduced with all the feeding devices compared to feeding from the ground. Further studies are needed to evaluate the long‐term effects of slow‐feeding devices on posture and mandibular angle, taking into consideration animals with different morphologies. [ABSTRACT FROM AUTHOR]

Desminopathy R350P is a human myopathy that is characterized by the progressive loss of muscle fiber organization. This results in the loss of muscle size, mobility, and strength. In desminopathy, inflammation affects muscle homeostasis and repair, and contributes to progressive muscle deterioration. Mitochondria morphology was also suggested to affect desminopathy progression. Epicatechin (Epi)—a natural compound found in cacao—has been proposed to regulate inflammatory signaling and mitochondria morphology in human and animal models. Hence, we hypothesize chronic Epi consumption to improve inflammatory pathway and mitochondria morphology in the peripheral blood mononuclear cells (PBMCs) of a desminopathy R350P patient. We found that 12 weeks of Epi consumption partially restored TRL4 signaling, indicative of inflammatory signaling and mitochondria morphology in the desminopathy patient. Moreover, Epi consumption improved blood health parameters, including reduced HOMA‐IR and IL‐6 levels in the desminopathy patient. This indicates that Epi consumption could be a useful tool to slow disease progression in desminopathy patients. [ABSTRACT FROM AUTHOR]

Using different three-drug immunosuppressive treatment regimens in a rat model, we aimed to determine the effects of long-term therapy on metalloproteinase-2 and metalloproteinase-9 activity and the expression of their inhibitors, as well as to assess the morphology of the animals' cardiac tissue. Our results suggest that chronic use of immunosuppressive drugs disrupts the balance between the activity of MMPs and TIMPs. Depending on the type of drug regimen used, this leads to abnormalities in the cardiac structure, collagen fiber accumulation, or cardiomyocyte hypertrophy. The information obtained in the present study allows us to conclude that the chronic treatment of rats with the most common clinical immunosuppressive regimens may contribute to abnormalities in the myocardial structure and function. The results presented in this study may serve as a prelude to more in-depth analyses and additional research into the optimal selection of an immunosuppressive treatment with the lowest possible risk of cardiovascular complications for patients receiving organ transplants. [ABSTRACT FROM AUTHOR]

This article examines the use of color in taxidermy at the Qing court in the 18th century. The court utilized various coloring techniques to bring life to inanimate forms, using synthetic materials to mimic the essence of life. Examples include taxidermized animal displays and mechanical devices adorned with colorful skins and feathers. The court's fascination with color and its transformative power highlights the belief that color is the essence of life. The article emphasizes the importance of coloration in preserving and enhancing the vibrancy of these artifacts over time. The text also provides a list of references and citations for further research on related topics such as the preservation of leather artifacts, the study of Manchu language, and the production of Buddhist costumes in the Qing Palace. [Extracted from the article]

Larvae represent a distinct life history stage in which animal morphology and behavior contrast strongly to adult organisms. This life history stage is a ubiquitous aspect of animal life cycles, particularly in the marine environment. In many species, the structure and function of the nervous system differ significantly between metamorphosed juveniles and larvae. However, the distribution and diversity of neural cell types in larval nervous systems remains incompletely known. Here, the expression of neurotransmitter and neuropeptide synthesis and transport genes in the bat star Patiria miniata is examined throughout larval development. This characterization of nervous system structure reveals three main neural regions with distinct but overlapping territories. These regions include a densely innervated anterior region, an enteric neural plexus, and neurons associated with the ciliary band. In the ciliary band, cholinergic cells are pervasive while dopaminergic, noradrenergic, and GABAergic cells show regional differences in their localization patterns. Furthermore, the distribution of some neural subtypes changes throughout larval development, suggesting that changes in nervous system structure align with shifting ecological priorities during different larval stages, before the development of the adult nervous system. While past work has described aspects of P. miniata larval nervous system structure, largely focusing on early developmental timepoints, this work provides a comprehensive description of neural cell type localization throughout the extensive larval period. Research Highlights: Mapping of neural subtypes in bat stars throughout larval development showed that the larval nervous system is composed of regionalized, nonuniform components that change throughout the larval period. [ABSTRACT FROM AUTHOR]

In the article "Response to Brian Besong," authors Alexander R. Pruss and Tyler Dalton McNabb engage in a philosophical discussion about the inclusion of animals in heaven. They counter Brian Besong's argument that animals in heaven would be inappropriate, asserting that a complete ecosystem, including the consumption of nutrients, is necessary for human fulfillment. The authors also consider the possibility of animals experiencing higher fulfillment in heaven. They address the argument against eating, drinking, and hunting in heaven, suggesting that prudishness and bodily processes will not hinder happiness in the afterlife. Additionally, they propose that hunting can be viewed as an interdependency and part of the purpose of certain animals. The article concludes by questioning the limited embodiment of human heavenly life and the exclusion of non-human animals from the blessed state. [Extracted from the article]

The study was conducted to evaluate the effect of Black olive (Canarium schweinfurthii) seed meal on the growth performance and carcass characteristics of broiler chickens. A total of 375-day-old Ross broiler chicks were allotted to five dietary treatments containing Canarium schweinfurthii seed meal at 0 g (T1), 250 g (T2), 500 g (T3), 750 g (T4) per Kg diet and oxytetracycline was used as positive control (T5) in a completely randomized design. All data generated were subjected to Analysis of Variance and significant difference among treatment means were compared using Duncan's Multiple Range Tests procedures. Growth performance results showed that final weight, weight gain, feed cost/Kg gain and feed conversion ratio were significantly (P<0.05) higher in birds fed diet supplemented with oxytetracycline (AGP) at both starter and finisher phases. Carcass characteristics of cut parts showed that birds on AGP had higher (P<0.05) drumstick and breast cut percentage compared to other dietary treatments. Organ weights such as liver, kidney and heart were significantly (P<0.05) higher in birds fed diets supplemented with Canarium schweinfurthii seed meal. Therefore, inclusion of Canarium schweinfurthii seed meal at 500 g/100kg diet improved broiler chickens' performance. [ABSTRACT FROM AUTHOR]

Two new species of the pseudoscorpion genus Pararoncus Chamberlin, 1938 are described from Korea. Detailed descriptions, habitus pictures, and illustrations of Pararoncus leonardi sp. nov. and P. taeyoungi sp. nov. are provided. The current distribution of the genus Pararoncus is mapped and a taxonomic key for all species is provided. [ABSTRACT FROM AUTHOR]

Simple Summary: This study finds that the skulls and mandibles of lions and tigers in predominantly European zoos differ in shape, but not size, from lions and tigers in the wild. The nature of the shape change found indicates that the mechanical influences of diet have influenced development. The majority of captive big cats used in this study have been fed partial or whole carcasses, which better replicate the mechanical properties of wild diets than softer prepared diets. We therefore speculate that additional mechanical stresses upon the skull and mandible such as the killing bite, manipulation such as dragging, and consumption of large prey in the wild have driven differentiation between the skulls of captive and wild big cats. It is important to understand these differences to better care for captive animals, enhance their survival in reintroductions, and to understand the importance of environmental factors in influencing an animal's size or shape. Zoo animals are crucial for conserving and potentially re-introducing species to the wild, yet it is known that the morphology of captive animals differs from that of wild animals. It is important to know how and why zoo and wild animal morphology differs to better care for captive animals and enhance their survival in reintroductions, and to understand how plasticity may influence morphology, which is supposedly indicative of evolutionary relationships. Using museum collections, we took 56 morphological measurements of skulls and mandibles from 617 captive and wild lions and tigers, reflecting each species' recent historical range. Linear morphometrics were used to identify differences in size and shape. Skull size does not differ between captive and wild lions and tigers, but skull and mandible shape does. Differences occur in regions associated with biting, indicating that diet has influenced forces acting upon the skull and mandible. The diets of captive big cats used in this study predominantly consisted of whole or partial carcasses, which closely resemble the mechanical properties of wild diets. Thus, we speculate that the additional impacts of killing, manipulating and consuming large prey in the wild have driven differentiation between captive and wild big cats. [ABSTRACT FROM AUTHOR]

The visual morphologies in animals are associated positively or negatively with production, adaptation, and behavioral characteristics. For rural farmers with limited records, these features are considered reliable phenotypic markers. Thus, this study was intended to explore the unique morphological features of indigenous chickens as a farmer's selection guide. A total of 1,060 adult chickens from four rural districts were investigated for nine morphological traits. The single-comb type, supposed to be of good fertility, was the most prevalent. Yellow and white shanks were predominant, followed by gray, greenish-yellow, green, and black pigmentations. Crested and silky chickens were more frequent in highland areas, whereas naked-neck chickens were observed in lowlands. Chickens of the lowlands have brighter, more uniform plumage colors and yellow shank, beak, and earlobe colors. On the other hand, the highland and mid-altitude chickens are characterized by dark, colorful combinations of various feather colors: laced plumage, white shank, rose, and deformed rose combs. Moreover, multiple correspondence analyses revealed that morphological features have special associations with districts. Thus, the observed morphological heterogeneity of the indigenous chickens can be used as a good indicator of selective breeding. Therefore, the associations of these characteristics with productivity qualities could be confirmed by quantitative and molecular tools. [ABSTRACT FROM AUTHOR]

This study aimed to identify behavioral and morphological traits of Nellore cattle that can influence calves' survival and pre-weaning performance. It was performed in two units of a large-scale cow–calf operation farm located in the Central-West region of Brazil, with 975 cow–calf dyads, by scoring cows' maternal protective behavior, body condition, horns, udder suspension (US), teat size (TS) and calves' vigor. TS was used to determine teat symmetry (TSm). The production unit where calves were born, their birth date, sex, and birth (BW) and weaning weights were also recorded. Cow ages at calving and production units were used to categorize the cows into twelve classes (Cage). The Shapiro–Wilk test alongside tables of frequencies were used to determine if continuous and categorical variables followed a normal distribution, respectively. Two datasets were considered in statistical analyses: (i) 975 dyad records were used to identify potential factors influencing calf survivability and (ii) 936 records of weaned calves were used to assess pre-weaning performance. Generalized linear models were used to assess the effect of target traits on the calves' weight adjusted for 210 days of age (W210, kg) and pre-weaning average daily gain (preADG, kg/day). Tukey's test was used to compare W210 and preADG means. Pearson's correlation coefficients were estimated between BW, W210 and preADG. Cage, US and the TSm significantly affected pre-weaning calf performance (p < 0.01). Older cows (independently of the production unit) and those with intermediate US and symmetrical teats weaned heavier calves. As expected, positive and significant correlation was observed between BW and preADG (r = 0.35), which, in turn, was highly correlated with W210 (r = 0.99). We concluded that none of the behavioral and morphological traits influenced calf survivability, but cow age, US and TSm impacted pre-weaning performance, with 8- to 11-year-old cows and those with intermediate US and symmetric teats leading to better calf performance at weaning. [ABSTRACT FROM AUTHOR]

Buffaloes (Bubalus bubalis) in Kaliakah Village are exploited in the makepung lampit tradition, in addition to food and agricultural tools. However, the population of buffaloes in Kaliakah Village is very small hence information on their morphology is needed to inform conservation efforts. This study aimed to describe qualitative and quantitative characters in the morphology of male and female buffaloes in Kaliakah Village. This survey study was analyzed on 24 male buffaloes and 30 female buffaloes. Qualitative characters observed consist of body shape, body color, eye color, and horn shape, while quantitative characters measured consist of chest girth (CG), leg length (LL), body length (BL), head length (HL), neck length (NL), tail length (TL), and estimated body weight (BW). The study results revealed variations in body shape (stocky and fat), body color (black and albino), and eye color (black and reddish black) in both male and female buffaloes. Male buffaloes have a CG of 167 ± 19.18 cm, LL of 57.63 ± 5.23 cm, BL of 128.13 ± 7.60 cm, HL of 31.38 ± 3.50 cm, NL of 49.25 ± 5.99 cm, TL of 61.88 ± 6.53 cm, and BW of 740.79 ± 198.59 kg. Female buffaloes had a CG of 160.40 ± 16.53 cm, LL of 57.17 ± 4.31 cm, BL of 127.39 ± 7.55 cm, HL of 30.72 ± 3.67 cm, NL of 72 ± 5.46 cm, TL of 60.56 ± 6.48 cm, and BW of 700.94 ± 168.87 kg. Based on ANOVA (one-way) analysis, there was no significant differences between the quantitative characters of male and female buffaloes. Based on an analysis of correlation, there are differences in quantitative characters that are strongly correlated among male and female buffaloes. [ABSTRACT FROM AUTHOR]

The branches originating from the arcus aortae formed by the aortae after exiting the heart differs between animals. In this study, it was aimed to examine the morphology of the arcus aortae in the badger (Meles meles). Two adult badgers, which died as a result of a traffic accident at different times and were brought to the anatomy laboratory. Thorax was dissected and the heart and aortae were exposed. It was observed the heart was located between 3rd-7th costae and was connected to the diaphragma with the ligamentum sternophericardiacum. It was determined that truncus brachiocephalicus and arteria subclavia sinistra was originated from the arcus aortae at the level of 4th costae. It was determined that the first branch of truncus brachiocephalicus was arteria carotis communis sinistra and then divided into arteria subclavia dextra and arteria carotis communis dextra, respectively. It was seen that arteria vertebralis, truncus costocervicalis, arteria cervicalis superficialis and arteria thoracica interna originated from arteria subclavia sinistra. Branches of arteria subclavia dextra were found to be similar to arteria subclavia sinistra. It was determined that the branches originating from the arcus aortae were similar to those in cats and dogs. It is thought that this study will contribute to the anatomical knowledge of the endangered badger, which is under protection. [ABSTRACT FROM AUTHOR]

Summary: Heterochrony—alteration to the rate or timing of development—is an important mechanism of trait differentiation associated with speciation. Heterochrony may explain the morphological divergence between two polyploid species, June sucker (Chasmistes liorus) and Utah sucker (Catostomus ardens). The larvae of both species have terminal mouths; however, as adults, June sucker and Utah sucker develop subterminal and ventral mouths, respectively. We document a difference in the timing of shape development and a corresponding change in the timing of gene expression, suggesting the distinctive mouth morphology in June suckers may result from paedomorphosis. Specifically, adult June suckers exhibit an intermediate mouth morphology between the larval (terminal) and ancestral (ventral) states. Endemic and sympatric Chasmistes/Catostomus pairs in two other lakes also are morphologically divergent, but genetically similar. These species pairs could have resulted from the differential expression of genes and corresponding divergence in trait development. Paedomorphosis may lead to adaptive diversification in Catostomids.

Background: Vappolotes Zhao & Li, 2019 is one of the troglophilous genera, with five known species. The previous description of V. tianjiayu from China was based solely on female specimens collected from caves in the Wuling Mountains in southern China without any males. New information: The present study, deals with the first record of the male of V. tianjiayu from its type locality: Guluo Cave. The validation of species is based on the morphological characteristics of both male and female. [ABSTRACT FROM AUTHOR]

This review summarizes information about the specific features that are characteristic of the centrosome and its relationship with the cell function of highly specialized cells, such as endotheliocytes. It is based on data from other researchers and our own long-term experience. The participation of the centrosome in the functional activity of these cells, including its involvement in the performance of the main barrier function of the endothelium, is discussed. According to modern concepts, the centrosome is a multifunctional complex and an integral element of a living cell; the functions of which are not limited only to the ability to polymerize microtubules. The location of the centrosome near the center of the interphase cell, the concentration of various regulatory proteins in it, the organization of the centrosome radial system of microtubules through which intracellular transport is carried out by motor proteins and the involvement of the centrosome in the process of the perception of the external signals and their transmission make this cellular structure a universal regulatory and distribution center, controlling the entire dynamic morphology of an animal cell. Drawing from modern data on the tissue-specific features of the centrosome's structure, we discuss the direct involvement of the centrosome in the performance of functions by specialized cells. [ABSTRACT FROM AUTHOR]

The findings of the current research study reveal that the liver of the white-breasted kingfisher exhibits a bilateral division, The right lobe exhibits a relatively greater size in comparison to the left lobe. The liver is completely concealed by an inconspicuous layer of connective tissue credited as the Glisson's capsule. The liver is formed out of hepatic cell cords or plates fact divide in a radial direction surrounding the central vein. The hepatic cords have been separated by a minute aperture pointed to as the blood sinusoids, which are marked by the presence of two distinct cell types along their lining the endothelial cell, and the Kupffer cell. In the current research study, 6 birds were used, and for the purpose of obtaining the liver, the birds were dissected, the liver was removed, and it was placed in a fixative solution for 24 hours. The bird under study has a liver consisting of two lobes, the right of which is the largest and contains the gall bladder. Histologically, the liver consists of a series of hepatic cords separated from each other by blood sinusoids. [ABSTRACT FROM AUTHOR]

Quadruped mammals can control the movement of their center of gravity when turning by skillfully utilizing their bodies to achieve adaptive turning movements. Interestingly, the low-speed turning behavior also changes depending on the animal's morphology. Therefore, this study aims to understand the control algorithm of low-speed turning, which can reproduce the turning behavior according to the location of the center of gravity. Specifically, we constructed a control algorithm based on the knowledge that animals steer with the leg closest to the center of gravity and verified it with a quadruped robot whose center of gravity could be adjusted. Consequently, the behavior observed in animals was successfully reproduced, with a stable and large turning angle per time when the proposed control algorithm was used. [ABSTRACT FROM AUTHOR]

Sulfonamide antibiotics in the environment not only disrupt the ecological balance but can also enter the human or animal body in various forms and cause harm. Therefore, exploring efficient methods to degrade sulfonamide antibiotics is crucial. In this study, we prepared biochar (BC) using corn straw, and TiO2/BC was obtained by doping different proportions of TiO2 into biochar with varying carbonization temperatures using the sol–gel method. Next, we investigated the degradation of sulfamethoxazole (SMX) in solution using the generated TiO2/BC under ultraviolet irradiation and studied the effects of various experimental parameters, such as the type of composite material, composite material addition, solution pH, and initial antibiotic concentration on SMX degradation. Under an initial SMX concentration of 30 mg/L, the composite with the best photocatalytic degradation performance was TiO2/BC-5-300 (i.e., 5 mL of TiO2 doping; 300 °C calcination temperature), with an addition amount of 0.02 g and a solution pH of 3. The degradation efficiency increased from 22.3% to 89%, and the most significant degradation effect occurred during the initial stage of photocatalytic degradation. In the TiO2/BC-5-300 treated SMX solution, the average rhizome length of bean sprouts was significantly higher than that of the untreated SMX solution and slightly lower than that of the deionized aqueous solution (3.05 cm < 3.85 cm < 4.05 cm). This confirmed that the photocatalytic degradation of SMX by the composite was effective and could efficiently reduce its impact on the growth of bean sprouts. This study provides essential data and theoretical support for using TiO2/BC in the treatment of antibiotic-contaminated wastewater. [ABSTRACT FROM AUTHOR]

Spermatozoa are the most variable cells across animal taxa. Phylogeny, speciation and postcopulatory sexual selection are typical factors that explain the sperm morphology variation in animals, and now these differences can also be explored on the level of genomic and proteomic differentiation. However, in non-model organisms, it is often difficult to employ these techniques because genomes are not yet available for most animal species, particularly for free-living songbirds (Passeriformes). Here, we employed label-free proteomics to generate proteomes in the zebra finch, a songbird species with an annotated genome and five wild-living songbirds representing five families within the Passerida clade, all with poorly known genomes. The results show that protein mapping of the new passerine proteomes to the zebra finch genome was successful, thus yielding highly similar protein identifications and a sufficient number of unique peptides in all the studied proteomes. Interestingly, while passerine sperm proteomes only partially reflect phylogenetic relationships between passerine families, midpiece length correlates with at least 59 proteins enriched in mitochondrial metabolism. Similar sperm proteomes seem to have evolved convergently across passerine lineages, potentially due to varying levels of sperm competition and marked variation in sperm sizes. [ABSTRACT FROM AUTHOR]

Studies of animal weaponry and defensive structures rarely take into consideration their underlying mechanical properties. We measured the compressive strength and thickness of the exoskeleton of the claw (chela) in two North American crayfish species, Faxonius virilis and F. limosus. We performed similar measures on the carapace, a body region not directly involved in agonistic contests. Males of both species generated significantly stronger maximum pinch forces than females. However, these differences can be attributed to differences in claw size between the sexes. The thickness (ultrastructure) of the claw exoskeleton was a significant predictor of its compressive strength and likely explained the difference in compressive strength we observed between the two species. Neither claw thickness nor claw compressive strength was correlated with maximum pinch force. Additionally, we found that crayfish body size was a strong predictor of carapace compressive strength and thickness, whereas sex was not. The claw had greater compressive strength and thickness than the corresponding values for the carapace. Our study shows that the mechanical properties of the crayfish exoskeleton are largely a function of size and highlights the need to integrate mechanical properties into studies of animal morphology and performance. [ABSTRACT FROM AUTHOR]

Reed bamboo is a major ecological and economic resource for many animals, including humans. Nonetheless, the influence of this plant's evolutionary role on the morphology of animal species remains unexplored. Here, we investigated the significance of bamboo habitats as ecological opportunities in shaping the skull morphology of bush frogs (Raorchestes) from the Western Ghats, Peninsular India. We applied a three‐dimensional (3D) geometric morphometric approach to capture the skull shape of 55 species of bush frogs. We visualized the skull shape variables in phylomorphospace with principal component analysis and performed phylogenetic generalized least‐squares analysis to assess the impact of cranial size (evolutionary allometry) and habitat (bamboo or non‐bamboo) on cranial shape. We quantified the morphological disparity between bamboo and non‐bamboo bush frogs' skull shape, and employed RRphylo, a phylogenetic ridge regression method, to access the evolutionary rate and rate shifts of skull shape change. The phylomorphospace delineated bamboo and non‐bamboo bush frogs. While cranial shape exhibited a significant but smaller association with size, its association with habitat type was non‐significant. We detected, however, significant differences in skull shape between the two frog groups, with bamboo frogs showing higher morphological disparity and a remarkable shift in the evolutionary rate of skull shape diversification. These findings underscore the role of reed bamboo in the evolution of skull shape in the radiation of frogs, endemic to the Western Ghats. We demonstrate that the association between the members of two distinct endemic clades (bamboo reeds and bamboo frogs) is the outcome of a deep‐time ecological opportunity that dates back to the Miocene. [ABSTRACT FROM AUTHOR]

"The Architecture of Evolution: The Science of Form in Twentieth-Century Evolutionary Biology" by Marco Tamborini explores the historical developments in Continental and anglophone approaches to animal morphology. Tamborini argues that morphology was reimagined as a transdisciplinary methodology at the boundary between biology, engineering, and literature. The book discusses the concept of "constraint" and its role in shaping late twentieth-century evolutionary biology. It also examines the influence of D'Arcy Thompson and Otto Schindewolf, as well as the interdisciplinary collaborations and tensions between German and anglophone researchers. However, the book does not delve into the dynamic formative processes of embryogenesis. [Extracted from the article]

The evolution of behaviour can both influence, and be influenced by, morphology. Recent advances in methods and data availability have facilitated broad-scale investigations of physical form and behavioural function in many contexts, but the relationship between animal morphology and object manipulation—particularly objects used in construction—remains largely unknown. Here, we employ a new global database of nest materials used by 5924 species of birds together with phylogenetically informed random forest models to evaluate the link between beak shape and these nest-building materials. We find that beak morphology, together with species diet and access to materials, can predict nest-material use above chance and with high accuracy (68–97%). Much of this relationship, however, is driven by phylogenetic signal and sampling biases. We therefore conclude that while variation in nest material use is linked with that of beak shape across bird species, these correlations are modulated by the ecological context and evolutionary history of these species. This article is part of the theme issue 'The evolutionary ecology of nests: a cross-taxon approach'. [ABSTRACT FROM AUTHOR]

The Euconulidae is a globally distributed land snail family but there is no record of this family from Thailand. In this study, we describe a new genus, Siamoconus gen. nov., based on comparative studies of the shell characteristics, external morphology of animals, radula, genital and spermatophore structures, and molecular phylogeny. We performed phylogenetic analyses of two mitochondrial gene fragment (cytochrome c oxidase I, COI; and 16S rRNA) and one nuclear (28S rRNA) gene fragment to clarify the relationships with other euconulid genera and its position in the superfamily Trochomorphoidea. We also analysed shell morphometrics, male genitalia and mantle pigmentation of Siamoconus gen. nov., confirming the status of three new species with keeled shells (S. boreas sp. nov., S. coleus sp. nov. and S. geotrochoides sp. nov.) and one new species with a rounded shell (S. destitutus sp. nov.). These new species are restricted to limestone areas in northern and north-eastern Thailand. We also re-examined the status of other genera in the superfamily Trochomorphoidea based on shell features, body pigmentation, radula, genital anatomy, spermatophore morphology, and a phylogenetic analysis of all available trochomorphoidean DNA sequences. Our analysis suggests that the family Geotrochidae, previously synonymised with the Trochomorphidae, should be resurrected to represent the genus Geotrochus from Borneo, and this family is retrieved as the sister clade of the Dyakiidae. ZooBank: urn:lsid:zoobank.org:pub:C5205F4E-5DDE-42E6-A532-761CAFE099C3 Euconulidae is one of the most diverse families in the superfamily Trochomorphoidea with a distribution that is almost world-wide but there is no record of this family from Thailand. We describe four new species of Siamoconus gen. nov., a new euconulid genus and also perform phylogenetic analyses of one nuclear and two mitochondrial gene fragments to clarify the systematic position of this new genus and the relationships to other genera in the superfamily Trochomorphoidea. In addition, the family Geotrochidae, previously synonymised with the Trochomorphidae, is resurrected as sister clade of the Dyakiidae within the superfamily Trochmorphoidea. [ABSTRACT FROM AUTHOR]

Caenorhabditis elegans (C. elegans) has served as a simple model organism to study dopaminergic neurodegeneration, as it enables quantitative analysis of cellular and sub-cellular morphologies in live animals. These isogenic nematodes have a rapid life cycle and transparent body, making high-throughput imaging and evaluation of fluorescently tagged neurons possible. However, the current state-of-the-art method for quantifying dopaminergic degeneration requires researchers to manually examine images and score dendrites into groups of varying levels of neurodegeneration severity, which is time consuming, subject to bias, and limited in data sensitivity. We aim to overcome the pitfalls of manual neuron scoring by developing an automated, unbiased image processing algorithm to quantify dopaminergic neurodegeneration in C. elegans. The algorithm can be used on images acquired with different microscopy setups and only requires two inputs: a maximum projection image of the four cephalic neurons in the C. elegans head and the pixel size of the user's camera. We validate the platform by detecting and quantifying neurodegeneration in nematodes exposed to rotenone, cold shock, and 6-hydroxydopamine using 63x epifluorescence, 63x confocal, and 40x epifluorescence microscopy, respectively. Analysis of tubby mutant worms with altered fat storage showed that, contrary to our hypothesis, increased adiposity did not sensitize to stressor-induced neurodegeneration. We further verify the accuracy of the algorithm by comparing code-generated, categorical degeneration results with manually scored dendrites of the same experiments. The platform, which detects 20 different metrics of neurodegeneration, can provide comparative insight into how each exposure affects dopaminergic neurodegeneration patterns. [ABSTRACT FROM AUTHOR]

Habitats are characterized by different local environmental conditions that influence both behavior and morphology of species, which can result in habitat‐dependent phenotypic differences among animals living in heterogeneous environments. We studied 3 alpine populations of Eurasian red squirrels (Sciurus vulgaris), 1 living in a marginal high‐elevation habitat at the edge of the species' altitudinal distribution, and 2 occurring in higher‐quality habitats. Here, we investigated whether squirrels living in the marginal area differed in 2 morphological parameters (body size and body mass) and/or in the expression of 4 personality traits estimated with an open field test and a mirror image stimulation test (activity, exploration, activity‐exploration, and social tendency). Furthermore, we tested whether within‐individual variance of the traits (behavioral plasticity) was higher in the edge habitat. Male squirrels in the edge habitat were smaller and weighed less than in the other study areas, while among females, size–habitat relationships were less marked. These sex‐specific patterns were explained by a strong association between body mass and reproductive success in female squirrels. Squirrels in the marginal habitat were more active, explorative, and had a more social personality than in the other habitats. However, in contrast to our predictions, behavioral plasticity was smaller in the marginal habitat, but only for the trait exploration. Our results suggest that animals choose the habitat that best fits their personality, and that habitat‐related differences in selective pressures may shape animals' morphology. [ABSTRACT FROM AUTHOR]

Octolasmis is a type of ectoparasite which is often found on the gills of lobsters. Octolasmis can also be a threat to lobster populations and can even cause high mortality rates. This study aims to detect the effect of different fixative solutions on the morphological characters of Octolasmis infesting the gills of scalloped spiny lobsters (Panulirus homarus). Samples of Octolasmis were collected from the gills of wildly-caught scalloped spiny lobsters in East Lombok, West Nusa Tenggara. The samples were immersed separately in two different fixative solution which are either absolute ethanol or formalin solution. After 7 days, morphological analysis of Octolasmis was analysed using an integrated optilab stereo binocular microscope and a lucida camera. The result showed that there were significant differences in the morphological condition of Octolasmis sp. including skin colour, organ appearance and size of the Octolasmis. In general, the result showed that formalin is better fixative solution. [ABSTRACT FROM AUTHOR]

Polish archaeological studies of the last century concerning the development of the settlement network and economic changes in the Lusatian culture (1350–450 BC) in areas of southern Poland prompted the theory that in the initial phase of this culture in Lesser Poland, the economic strategies were the same as in the Upper Silesia, the area from which the settlers came. The cultivation of the native economic model was possible because the Lusatian population migrating from Silesia in the south-east to Lesser Poland settled in environmentally similar areas (loess). This theory was built on the basis of general environmental observations and analyses of the historical ceramic material. To check the validity of the aforementioned thesis, I have analysed a number of faunal materials obtained from three large Lusatian settlements located in loess areas near Krakow. Osteological material was subjected to a comprehensive analysis taking into account the species composition, in terms of anatomy as well as age, gender and animal morphology. The results obtained showed that in addition to environmental pressure, cultural considerations and breeding traditions also had a determinative influence on the choice of the economic pattern. [ABSTRACT FROM AUTHOR]

The present article is dedicated to conative animal calls (CACs) in a Kalahari Khoe language, Tjwao. By using a prototype approach to categorization, the authors test the Tjwao CACs for their compliance with the prototype of CACs posited recently in scholarly literature. The authors conclude that Tjwao CACs largely conform to the pragma-semantic, phonetic, and morphological properties associated with CACs across languages. In light of the Tjwao data, a few refinements are also proposed. These concern the potential prevalence of whistles as the most common sounds not included in the International Phonetic Alphabet, the correlation of summonses with replication and repetitions as well as front and/or close vowels, the higher frequency of summonses and dispersals among all semantic types of CACs, and the lesser extent of monosemy than previously claimed. [ABSTRACT FROM AUTHOR]

As a case study of the responses of natural history museums to changing scientific and funding environments, we analysed research publications of Australia's Natural History Museums (ANHMs) 1981–2020. Using Scopus, 9,923 relevant documents 1981–2020 were identified, mainly research papers but with a growing proportion of reviews. The number of documents published increased over tenfold from 39 (1981) to 553 (2020), likely driven by collaborations (rising from 28.5% of documents 1981–1985 to 87.2% of documents 2016–2020), contributions from retired staff, and volunteer support. The mean length of documents (pages) ranged from a low of 15.3 in 2001–2005 to a high of 17.4 in 1991–1995, but this statistically significant result was trivial in practical terms. The sources (i.e., journals, book titles, conference proceedings) in which ANHM authors published changed over time, with growing proportions of publications in journals covering molecular ecology/phylogenetics and biological conservation. We identified the major areas of study canvassed within the corpus of publications by developing structural topic models based on patterns of word use in document titles, abstracts and keyword lists. The topics discovered included study subjects traditional for natural history museums (new taxa, phylogeny, systematics, animal morphology, palaeontology, minerals), new directions (molecular genetics, ecology, biological conservation) and marine biology (probably reflecting Australia's large coastline). Most citations came from Australia, USA and UK, although in 2016–2020 only 27.9% of citing documents included an Australian author. Growth in numbers of documents and collaborations, as well as use of documents internationally over a period of great change in scientific and funding environments, indicate an enduring legacy of ANHM research, grounded on the intrinsic value of the collections. [ABSTRACT FROM AUTHOR]