1. Transcriptional Response of Peripheral Blood Mononuclear Cells from Cattle Infected with Mycobacterium bovis
- Author
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Fabiana Bigi, Federico Carlos Blanco, Marcelo Abel Soria, and María Verónica Bianco
- Subjects
GZMA GENE ,Transcription, Genetic ,Microarray ,Microarrays ,Apoptosis ,ANIMAL EXPERIMENT ,AREGB GENE ,Immunopathology ,BOS ,lcsh:Science ,ADAMTSL4 GENE ,TUBERCULOSIS, BOVINE ,CXCL10 GENE ,Oligonucleotide Array Sequence Analysis ,Mycobacterium bovis ,BIOLOGICAL MARKERS ,FYVE GENE ,PYCARD GENE ,FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR ,Endocytosis ,Bacterial Pathogens ,INTERLEUKIN 22 ,RESISTIN ,TGM3 GENE ,Medicine ,SASH1 GENE ,IL1RN GENE ,UPREGULATION ,HYALURONAN SYNTHASE 2 ,LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN ,MYCOBACTERIUM BOVIS ,Microbiology ,Mycobacterium tuberculosis ,MATRIX METALLOPROTEINASE 19 ,CATTLE DISEASE ,MICROARRAY ANALYSIS ,CCL8 GENE ,NONHUMAN ,LEUKOCYTES, MONONUCLEAR ,CCL2 GENE ,Biology ,Microbial Pathogens ,TREM1 GENE ,TRANSCRIPTION FACTOR FYVE ,BOVINE TUBERCULOSIS ,TRANSFORMING GROWTH FACTOR BETA ,lcsh:R ,RETN GENE ,ANIMALS ,Computational Biology ,CSF3R GENE ,HAS2 GENE ,AMPHIREGULIN B ,TRANSGLUTAMINASE 3 ,Immunology ,TGFBI GENE ,Leukocytes, Mononuclear ,lcsh:Q ,MANNOSE RECEPTOR C TYPE 1 ,Cattle ,Veterinary Science ,THBS1 GENE ,ARHGAP21 GENE ,Biomarkers ,GELATINASE B ,Bacterial Diseases ,GENETIC VARIABILITY ,PROTEIN DEGRADATION ,MODELS, GENETIC ,Veterinary Microbiology ,CD14 ANTIGEN ,FCGR1A GENE ,REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION ,CATTLE ,lcsh:Medicine ,UROKINASE RECEPTOR ,CHEMOKINE RECEPTOR CXCR2 ,ANIMAL MODEL ,UNCLASSIFIED DRUG ,CELL TRANSPORT ,NFAM1 GENE ,IL 18 GENE ,IL 6 GENE ,CD14 GENE ,VLDLR GENE ,GENE EXPRESSION REGULATION, BACTERIAL ,TRANSCRIPTION FACTOR ,TRANSCRIPTION FACTOR GZMA ,THROMBOCYTE FACTOR 4 ,SH3BP4 GENE ,Multidisciplinary ,PF4 GENE ,S100A9 GENE ,biology ,CCR1 GENE ,IL 1R GENE ,NRP1 GENE ,CXCL9 GENE ,MAPK14 GENE ,CXCR2 GENE ,APOPTOSIS ,Host-Pathogen Interaction ,Bovine genome ,Infectious Diseases ,PLAUR GENE ,BIOLOGICAL MARKER ,SH3 DOMAIN BINDING PROTEIN 4 ,ADAM8 GENE ,MMP9 GENE ,Research Article ,FCAR GENE ,TRANSCRIPTION, GENETIC ,ENDOCYTOSIS ,IL13RA1 GENE ,MAPK13 GENE ,INTERLEUKIN 1 RECEPTOR ,MMP3 GENE ,TYROBP GENE ,IL1R1 GENE ,Peripheral blood mononuclear cell ,DOWN REGULATION ,Mycobacterium ,Immune system ,INFLAMMATION ,OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS ,BOVINAE ,ANIMAL TISSUE ,IMMUNE RESPONSE ,MRC1 GENE ,DEFBIO GENE ,QUANTITATIVE ANALYSIS ,Animals ,LOC533894 GENE ,MMP19 GENE ,MYD88 GENE ,GENE EXPRESSION PROFILING ,CXCL9 CHEMOKINE ,NEUROPILIN 1 ,Inflammation ,Gram Positive ,LOC508666 GENE ,Models, Genetic ,INTERLEUKIN 13 RECEPTOR ALPHA1 ,Gene Expression Profiling ,Gene Expression Regulation, Bacterial ,biology.organism_classification ,GENE ,TRANSCRIPTION FACTOR THBS1 ,Gene expression profiling ,Emerging Infectious Diseases ,VERY LOW DENSITY LIPOPROTEIN RECEPTOR ,ANIMAL CELL ,PERIPHERAL BLOOD MONONUCLEAR CELL ,IL22 GENE ,MONOCYTE CHEMOTACTIC PROTEIN 2 ,RNA ,CALGRANULIN B ,Tuberculosis, Bovine ,ECM1 GENE - Abstract
e41066 Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis.
- Published
- 2012
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