1. Oligodendrocyte progenitor cells proliferate and survive in an immature state following treatment with an axolemma-enriched fraction
- Author
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Julie K. Nelson, Shu-Jen Chen, Sara G. Becker-Catania, George H. DeVries, and Shantel Olivares
- Subjects
MAPK/ERK pathway ,AEF, axolemma-enriched fraction ,Hot Temperature ,LIF, leukaemia inhibitory factor ,Cellular differentiation ,Basic fibroblast growth factor ,Cell Count ,multiple sclerosis ,DSHB, Developmental Studies Hybridoma Bank ,MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,CREB, cAMP-response-element-binding protein ,bFGF, basic fibroblast growth factor ,Trypsin ,Cells, Cultured ,Neurons ,General Neuroscience ,Stem Cells ,Cell Differentiation ,Chromatography, Agarose ,axolemma-enriched fraction (AEF) ,Cellular Structures ,S11 ,Cell biology ,Oligodendroglia ,BDNF, brain-derived neurotrophic factor ,Stem cell ,Mitogen-Activated Protein Kinases ,F-12, Ham's F12 nutrient medium ,acidic fibroblast growth factor (aFGF) ,Research Article ,NRG, neuregulin ,Biology ,S8 ,CNS, central nervous system ,ERK, extracellular-signal-regulated kinase ,S5 ,lcsh:RC321-571 ,DMEM–F12, Dulbecco's modified Eagle medium nutrient mixture F-12 ,Animals ,AEF-SE, soluble 2.0 M NaCl extract of the AEF ,Protein kinase A ,Protein kinase B ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Cell Proliferation ,Cell growth ,G3PDH, glyceraldehyde-3-phosphate dehydrogenase ,CNS trophic factors ,Oligodendrocyte differentiation ,oligodendrocyte progenitor cell ,GFAP, glial fibrillary acidic protein ,OPC, oligodendrocyte progenitor cell ,axonal–oligodendrocyte signalling ,BCA, bicinchoninic acid ,Rats ,stomatognathic diseases ,oligodendrocyte differentiation ,chemistry ,nervous system ,Animals, Newborn ,Akt, protein kinase B ,aFGF, acidic fibroblast growth factor ,Neurology (clinical) ,Schwann Cells ,FCS, fetal calf serum ,GalC, galactosylcerebroside ,Mitogens ,RIPA buffer, radio immunoprecipitation assay buffer ,CNPase, 2′,3′-cyclic nucleotide 3′-phosphodiesterase ,Proto-Oncogene Proteins c-akt ,MAPK, mitogen-activated protein kinase ,DAPI, 4′,6-diamidino-2-phenylindole - Abstract
The ability of an AEF (axolemma-enriched fraction) to influence the proliferation, survival and differentiation of OPC (oligodendrocyte progenitor cells) was evaluated. Following addition of AEF to cultured OPC, the AEF associated with the outer surface of OPC so that subsequent metabolic events were likely mediated by direct AEF-OPC contact. Addition of AEF to the cultured OPC resulted in a dose- and time-dependent increase in proliferation that was partially dependent on Akt (protein kinase B) and MAPK (mitogen-activated protein kinase) activation. The major mitogen in an AEF-SE (soluble 2.0 M NaCl extract of the AEF) was identified as aFGF (acidic fibroblast growth factor) and accounted for 50% of the mitogenicity. The remaining 50% of the mitogenicity had properties consistent with bFGF (basic fibroblast growth factor) but was not unequivocally identified. Under conditions that limit the survival of OPC in culture, AEF treatment prolonged the survival of the OPC. Antigenic and morphological examination of the AEF-treated OPC indicated that the AEF treatment helped the OPC survive in a more immature state. The potential downstream metabolic pathways potentially activated in OPC by AEF and the consequences of these activated pathways are discussed. The results of these studies are consistent with the view that direct contact of axons with OPC stimulates their proliferation and survival while preventing their differentiation.
- Published
- 2011