1. Inhibitory Effects of Olea europaea Leaf Extract on Mesenchymal Transition Mechanism in Glioblastoma Cells
- Author
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Cagla Tekin, Secil Ak Aksoy, Unal Egeli, Gulsah Cecener, Melis Mutlu, Berrin Tunca, Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı., Mutlu, Melis, Tunca, Berrin, Aksoy, Seçil, Tekin, Çağla, Egeli, Ünal, Çeçener, Gülşah, AAH-1420-2021, ABI-6078-2020, and AAH-8540-2021
- Subjects
0301 basic medicine ,Cancer Research ,Concomitant ,Unclassified drug ,Medicine (miscellaneous) ,Expression ,Antiproliferative activity ,Snail ,Nerve cell adhesion molecule ,0302 clinical medicine ,Invasion ,Nutrition and Dietetics ,Transition (genetics) ,biology ,Plant leaf ,Cell migration ,Wound healing assay ,Transcription factor ZEB1 ,Cadherins ,Epithelial-mesenchymal transition ,Stem-cells ,Cell invasion ,Plant extract ,Antineoplastic agent ,Migration inhibition ,Oncology ,030220 oncology & carcinogenesis ,Uvomorulin ,Drug mechanism ,Human ,Transcription factor twist ,Efficacy ,Inhibitory postsynaptic potential ,Methylation ,Adjuvant temozolomide ,Article ,Association ,03 medical and health sciences ,Transcription factor snail ,Glioblastoma cell line ,Olea ,biology.animal ,Epithelial Mesenchymal Transition ,Transcription Factor ZEB1 ,Metastasis ,Genetics ,medicine ,Humans ,Nutrition & dietetics ,Snail family transcription factors ,Transcription factor ,Antineoplastic activity ,030109 nutrition & dietetics ,Radiotherapy ,Mesenchymal stem cell ,Plant extracts ,Tumor cell line ,Nonhuman ,medicine.disease ,Brain tumor ,Brain neoplasms ,Cell line, tumor ,Human cell ,Cadherin ,Cancer research ,Protein expression ,Olea europaea extract ,Glioblastoma ,Epithelial mesenchymal transition ,Wound healing ,Olive tree ,Olive oil - Abstract
Background: Glioblastoma (GB) is the most aggressive form of brain tumor. Despite the current treatment methods, the survival rate of patients is very low. Therefore, there is a need to develop new therapeutic agents. The migration and invasion capacity of GB cells is related to mesenchymal transition (MT) mechanism. Materials and Methods: The effect of OLE on MT was determined by analysis of the Twist, Snail, Zeb1, N-cadherin and E-cadherin genes in the EMT mechanism. The effect of OLE on cell migration was determined by wound healing test. Results: 2 mg/ml OLE reduced Twist, Snail, Zeb1 and N-cadherin expression and the combination of OLE + TMZ (2 mg/ml OLE + 350 mM TMZ) increased E-cadherin and reduced Twist, Zeb1 and N-cadherin. In addition, co-treatment with OLE increased TMZ-induced anti-invasion properties thought suppressing transcription factors of MT mechanism. Conclusion: OLE can enhance the anti-MT activities of TMZ against GB and provide strong evidence that combined treatment with OLE and TMZ has the potential to be an effective alternative approach in GB therapy.
- Published
- 2020
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