7 results on '"A.C.P. Gomes"'
Search Results
2. Evaluation of encapsulated anethole and carvone in lambs artificially- and naturally-infected with Haemonchus contortus
- Author
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Jorge F. S. Ferreira, A.C.P. Gomes, Gunta Gutmanis, Leandro Rodrigues, Helder Louvandini, Luciana Morita Katiki, Mauro Sartori Bueno, L. Ziegelmeyer, Alessandro Francisco Talamini do Amarante, R.C. Araujo, Cecília José Veríssimo, Instituto de Zootecnia (IZ/SAA), GRASP Ind. e Com. LTDA, Universidade de São Paulo (USP), US Salinity Lab (USDA-ARS), and Universidade Estadual Paulista (Unesp)
- Subjects
Male ,Allylbenzene Derivatives ,Weight Gain ,Essential oil ,Eating ,Feces ,Random Allocation ,chemistry.chemical_compound ,Urea ,education.field_of_study ,biology ,Abomasum ,Drug Synergism ,gamma-Glutamyltransferase ,General Medicine ,Drug Resistance, Multiple ,Infectious Diseases ,Creatinine ,Toxicity ,Female ,Haemonchus ,medicine.symptom ,Anethole ,Haemonchus contortus ,Immunology ,Population ,Sheep Diseases ,Capsules ,Cyclohexane Monoterpenes ,Anisoles ,Animal science ,medicine ,Animals ,Dry matter ,Aspartate Aminotransferases ,education ,Parasite Egg Count ,Carvone ,Sheep ,biology.organism_classification ,Fertility ,chemistry ,Erythrocyte Count ,Monoterpenes ,Encapsulation ,Parasitology ,Haemonchiasis ,Weight gain - Abstract
Made available in DSpace on 2019-10-06T15:31:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-02-01 Molecules from natural sources, such as essential oils, have shown activity against parasites in vitro, but have not yet been explored extensively in vivo. Anethole and carvone (10% each), encapsulated with 80% of a solid matrix, referred to as EO (encapsulated oils), were tested in vivo in 2 experiments. In Experiment 1: Lambs were artificially infected with multidrug resistant Haemonchus contortus, or left uninfected, and treated (or not) with 50 mg/kg bw (body weight) of EO in a controlled environment. Thirty-two male lambs were kept in individual cages for a period of 45 days, after which animals were evaluated for parasitological, hematological, toxicological, and nutritional parameters. After 45 days of treatment, EO at 50 mg/kg bw provided a significant (P ≤ 0.05) reduction in fecal egg count (FEC). Although FEC was reduced, animals from both treatments had similar counts of total adult worms. The low FEC was caused probably by a significant reduction (P ≤ 0.05) in both male worm size and female fecundity. Dry matter intake of uninfected controls was significantly (P ≤ 0.05) reduced, although no toxicity was observed in treated animals. Thus, in Experiment 2, conducted for five months we used an EO dose of 20 mg/kg bw. Thirty-four weaned lambs, free of parasites, were divided in two groups and kept in collective pens. One group received EO at 20 mg/kg bw mixed with concentrate for 5 months and the other was kept as a control group (CTL). Parasitological and hematological parameters as well as body weight were evaluated. In the first 2.5 months, CTL and EO groups were confined, and both presented similar clinical parameters. Then, animals were allotted to graze on contaminated pastures to acquire natural infection for the next 2.5 months. The infection was patent after 25 days and both groups had similar decreases in weight gain, increases in FEC, and decreases in blood parameters. Coprocultures from CTL and EO groups established that parasite population was 90% Haemonchus sp. We concluded that the technology of encapsulation is safe and practical to deliver to lambs at the farm level and anethole and carvone at 50 mg/kg bw caused a significant decrease in FEC and, consequently, in pasture contamination by free living stages of H. contortus. However, EO at 20 mg/kg bw was not effective to prevent or treat sheep naturally-infected with gastrointestinal nematodes. Instituto de Zootecnia (IZ/SAA), Rua Heitor Penteado 56 GRASP Ind. e Com. LTDA, Av. Juscelino Kubitschek de Oliveira Centro de Energia Nuclear na Agricultura (CENA/USP), Rua do Centenário 303 US Salinity Lab (USDA-ARS), 450 W. Big Springs Rd. Instituto de Biociências (UNESP) Instituto de Biociências (UNESP)
- Published
- 2019
3. Inclusion complex and nanoclusters of cyclodextrin to increase the solubility and efficacy of albendazole
- Author
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Cecília José Veríssimo, Leticia Norma Carpentieri Rodrigues, Luciana Morita Katiki, A.C.P. Gomes, Jorge F. S. Ferreira, P. A. Pacheco, Helder Louvandini, and Ricardo Lopes Dias da Costa
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Male ,0301 basic medicine ,Benzimidazole ,Nematoda ,Drug Compounding ,Biological Availability ,Sheep Diseases ,Biology ,Albendazole ,Body weight ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,medicine ,Animals ,Anthelmintic ,Intestinal Diseases, Parasitic ,Solubility ,Nematode Infections ,Anthelmintics ,chemistry.chemical_classification ,Cyclodextrins ,Sheep ,Chromatography ,Antiparasitic Agents ,General Veterinary ,Cyclodextrin ,beta-Cyclodextrins ,technology, industry, and agriculture ,Povidone ,General Medicine ,2-Hydroxypropyl-beta-cyclodextrin ,Bioavailability ,030104 developmental biology ,Infectious Diseases ,chemistry ,030220 oncology & carcinogenesis ,Insect Science ,Nanoparticles ,Parasitology ,medicine.drug - Abstract
Albendazole (ABZ), a benzimidazole widely used to control gastrointestinal parasites, is poorly soluble in water, resulting in variable and incomplete bioavailability. This has favored the appearance ABZ-resistant nematodes and, consequently, an increase in its clinical ineffectiveness. Among the pharmaceutical techniques developed to increase drug efficacy, cyclodextrins (CDs) and other polymers have been extensively used with water-insoluble pharmaceutical drugs to increase their solubility and availability. Our objective was to prepare ABZ formulations, including β-cyclodextrin (βCD) or hydroxypropyl-β-cyclodextrin (HPβCD), associated or not to the water-soluble polymer polyvinylpyrrolidone (PVP). These formulations had their solubility and anthelmintic effect both evaluated in vitro. Also, their anthelmintic efficacy was evaluated in lambs naturally infected with gastrointestinal nematodes (GIN) through the fecal egg count (FEC) reduction test. In vitro, the complex ABZ/HPβCD had higher solubility than ABZ/βCD. The addition of PVP to the complexes increased solubility and dissolution rates more effectively for ABZ/HPβCD than for ABZ/βCD. In vivo, 48 lambs naturally infected with GIN were divided into six experimental groups: control, ABZ, ABZ/βCD, ABZ/βCD-PVP, ABZ/HPβCD, and ABZ/HPβCD-PVP. Each treated animal received 10 mg/kg of body weight (based on the ABZ dose) for three consecutive days. After 10 days of the last administered dose, treatment efficacy was calculated. The efficacy values were as follows: ABZ (70.33%), ABZ/βCD (85.33%), ABZ/βCD-PVP (82.86%), ABZ/HPβCD (78.37%), and ABZ/HPβCD-PVP (43.79%). In vitro, ABZ/HPβCD and ABZ/HPβCD-PVP had high solubility and dissolution rates. In vivo, although the efficacies of ABZ/βCD, ABZ/βCD-PVP, and ABZ/HPβCD increased slightly when compared to pure ABZ, this increase was not significant (P > 0.05).
- Published
- 2018
4. Terminalia catappa: Chemical composition, in vitro and in vivo effects on Haemonchus contortus
- Author
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Helder Louvandini, Luciana Morita Katiki, Cecília José Veríssimo, A Piza, Leandro Rodrigues, P. A. Pacheco, A.M.E. Barbieri, Jorge F. S. Ferreira, A.C.P. Gomes, and Gunta Gutmanis
- Subjects
0301 basic medicine ,Male ,Sheep Diseases ,Egg hatch assay ,Abomasum ,03 medical and health sciences ,chemistry.chemical_compound ,parasitic diseases ,Botany ,medicine ,Animals ,Anthelmintic ,Food science ,Parasite Egg Count ,Anthelmintics ,Leucocyanidin ,Sheep ,General Veterinary ,biology ,Plant Extracts ,Terminalia ,food and beverages ,General Medicine ,030108 mycology & parasitology ,biology.organism_classification ,Plant Leaves ,Phytochemical ,chemistry ,Proanthocyanidin ,Fruit ,Seeds ,Parasitology ,Haemonchus ,Haemonchiasis ,Haemonchus contortus ,medicine.drug ,Phytotherapy - Abstract
Haemonchus contortus is the most important nematode in small ruminant systems, and has developed tolerance to all commercial anthelmintics in several countries. In vitro (egg hatch assay) and in vivo tests were performed with a multidrug strain of Haemonchus contortus using Terminalia catappa leaf, fruit pulp, and seed extracts (in vitro), or pulp and seed powder in lambs experimentally infected with H. contortus. Crude extracts from leaves, fruit pulp and seeds obtained with 70% acetone were lyophilized until used. In vitro, the extracts had LC50=2.48μg/mL (seeds), LC50=4.62μg/mL (pulp), and LC50=20μg/mL (leaves). In vitro, seed and pulp extracts had LC50 similar to Thiabendazole (LC50=1.31μg/mL). Condensed tannins were more concentrated in pulp extract (183.92g of leucocyanidin/kg dry matter) than in either leaf (4.6g) or seed (35.13g) extracts. Phytochemical tests established that all extracts contained alkaloids, flavonoids, saponins, phenols, and terpenoids. Based on these results, in vivo tests were performed to evaluate the anthelmintic activity of T. catappa whole fruit (pulp+seed) powder. Male Santa Ines lambs were artificially infected with multidrug-resistant H. contortus and divided, according to similar fecal egg count (FEC) and weight, into two groups: Control (infected/untreated) and treated (infected/treated with whole fruit powder). Whole fruit powder was mixed with concentrate and provided at 2g/kg of body weight (BW) for five days. After treatment, parasitological analysis (FEC and egg hatch assay), renal profile (urea and creatinine), liver profile (aspartate aminotransferase) and BW were determined. In vitro (based on LC50), seed/pulp extracts had ovicidal effect similar to Thiabendazole but whole fruit powder had no anthelmintic effect on adult nematodes in the abomasum. We discuss the plausible causes of the lack of in vivo activity.
- Published
- 2017
5. Reúso do Lodo na Cultura do Pimentão no Município de Barro - CE
- Author
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S.L. Silva, V.S. Santos, A.C.P. Gomes, J.A. Pereira, and F.K.S. Alves
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- 2014
6. Acompanhamento do Desenvolvimento da Alface (Lactuca sativa L.) na Hidropônia como Alternativa para o Semiárido
- Author
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F. Valdelice, J.A.G. Menezes, A.C.N. Silva, V.S. Santos, L.G. Rodrigues, and A.C.P. Gomes
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- 2014
7. Produção de Mudas com Efluente Tratado no Município Juazeiro do Norte - CE
- Author
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F.W.D. Sousa, L.G. Rodrigues, V.S. Santos, J.A. Pereira, A.C.P. Gomes, and A.C.N. Silva
- Published
- 2014
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