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Emerging research points to high rates of major psychiatric disorders and chronic medical conditions among autistic adults. However, the unique healthcare needs of autistic adults are often overlooked, perhaps due to limitations in population-level surveillance and widespread public misconceptions about autism in adulthood. Understanding the mechanisms underlying this prevalence of poor health outcomes in autistic adults requires an exploration into the lived experiences of autistic adults across their lifespans. This study uses a qualitative approach to investigate the healthcare experiences of autistic adults. We conducted semi-structured interviews with 23 autistic adults in the United States, ranging in age from 18 to 56 years, seven of whom identified as nonbinary or gender non-conforming. We asked autistic adults about how they manage their health and interact with healthcare systems. Through thematic analysis of the data, barriers to receipt of quality care for autistic adults were identified. Results were then conceptualized through the Systems Engineering Initiative for Patient Safety model of work system and patient safety. Through this model, this study describes the interactions between autistic adults and their healthcare systems, pointing to specific areas for improvement and intervention. Overall, findings advocate for a system-level approach to improving health outcomes of autistic adults.

Purpose: Although California school discipline policy changes over the past decade have resulted in significant drops in suspension rates, scholars have found that racial disproportionality in punitive discipline persists for Black, Indigenous, and at times Latinx students. This study utilizes racial capitalism as an analytical framework to examine the mechanisms and justifications for racially disproportionate discipline across three distinct California political economies. Research Methods/Approach: Drawing on a subset of qualitative comparative case study data of five schools taken from a larger 5-year study of 31 schools in 17 California districts with distinct racial and economic composition, we investigate the phenomena of Black, Indigenous, and Latinx student disposability--those who continue to experience exclusion in a time of progressive school discipline reform. Findings: We find that (1) student disposability through discipline was not only intertwined with dominant local narratives about racial groups but also tied to a larger system of racial capitalism reliant on the racialized differentiation of students within every geographical location, and (2) when progressive alternative approaches to discipline fail, these reforms uncover rooted antiblackness, anti-indigeneity, and a gang imaginary such that students who fail or refuse to assimilate to the underlying labor-sorting function of US schools are considered disposable. Implications: Our findings highlight the need to center and elevate disposable youth brilliance in decision making, the importance of building collective movements against racial capitalism's grip on schools, and the obligation to build education beyond racial capitalism.

Enhancing language literacy is one of the most prioritized learning targets of the K-12 curriculum in the Philippines. Several language acquisition, learning, teaching and assessing principles to which the present Language Arts and Multiliteracies Curriculum (LAMC) is anchored on, are consistently improved to forward maximum and effective instructional efforts in enhancing learners' communicative competence and multiliteracies. At the elementary level, teachers apply innovative pedagogical strategies to develop learners' macro skills which are relevant to have a meaningful interaction with others in a multicultural society. However, this language literacy enhancement is considered as a challenging task for the Indigenous Peoples Education (IPEd) teachers due to several constraints that may have influenced the indigenous learners' language learning competence. Using the qualitative-case study research design, the responses of the selected IPEd teachers from the semistructured interviews were examined. The thematic analysis was utilized to determine the challenges encountered in teaching language literacy among indigenous learners in Agusan Del Sur, Philippines. On the other hand, the Provus Discrepancy model was used to determine the gaps between the current language literacy performance and outcomes and the language teaching standards enshrined in LAMC. The results showed that IPEd teachers commonly struggled with enhancing learners' viewing skills and digital literacy, developing reading comprehension, improving writing skills, contextualizing lessons, following spiral progression in language, and teaching orthography and grammar. Moreover, the results showed that these encountered struggles became more complicated due to the gaps in language learning standards, instructional learning support, learners' literacy and readiness level, and teachers' competence and strategies used. [The publication year (2022) shown on the PDF is incorrect. The correct publication year is 2023.]

During the COVID-19 pandemic, college faculty took many different actions to support student success during the transition to online instruction. However, the conclusions we draw about the "impact" of these adaptations and their implications for racial equity may vary depending on the outcome measures we examine. We explore this possibility through a mixed-methods study of 10 courses taught at a Hispanic-Serving Institution in the United States in Spring 2020. First, using qualitative analytical methods, we identify five types of instructional adaptations students noticed their instructors made early in the pandemic. Second, we use quantitative methods to uncover associations between these instructional adaptations and several student- and course level outcome variables. While all five instructional adaptations were perceived as beneficial by students, only two--"ensuring access to class resources" and "ensuring access to instructor time"--were significantly correlated with racially marginalized students' self-reported motivational and personal gains from their coursework. None of the adaptations were significantly associated with more equitable course outcomes, however. We discuss the implications of these findings and the differing narratives they imply for researchers, practitioners, and policymakers.

Students of color, first-generation, and residents of communities experiencing disinvestment--who aspire to obtain meaningful postsecondary degrees, career success, financial security, and social mobility--face tremendous challenges in navigating science, technology, engineering, math, and health science (STEM-H) education and career sectors. The Rush Education and Career Hub (REACH) utilizes a place-based workforce intermediary model that joins an academic medical center with academic institutions, community-based organizations, and employers to increase and broaden STEM-H participation. REACH provides innovative, hands-on learning for underrepresented youth cradle-to-career to increase postsecondary achievement and diversity in STEM-H professions through: (1) early and sustained access to high quality STEM-H education experiences, (2) paid work-based learning paired with professional development, (3) the ability to earn recognized industry credentials, (4) mentorship to facilitate participant engagement and promote program completion, and (5) OutREACH community engagement that provides information, resources and wraparound supports for students, their families, and the community. Preliminary findings suggest that this model inspires and prepares young people for success. REACH offers institutions an equity-centered community-engaged framework for cultivating education and workforce partnerships to foster opportunities, support, and the relationships necessary to thrive in STEM-H for those who have historically been denied.

We study a binary blend of telechelic homopolymers that can form reversible AB-type bonds at the chain ends. Reversibly bonding polymers display novel material properties, including thermal tunability and self-healing, that are not found in conventional covalently bonded polymers. Previous studies of reversibly bonding polymer systems have been limited by the computational demand of accounting for an infinite number of possible reaction products in a spatially inhomogeneous, self-assembled structure. We demonstrate that newly developed theoretical models and numerical methods enable the simultaneous computation of phase equilibrium, reaction equilibrium, and self-assembly via self-consistent field theory. Phase diagrams are computed at a variety of physically relevant conditions and are compared with nonreactive analogues as well as previous experimental studies of telechelic polymer blends.

Species delimitation of Culicoides complex species can be challenging. Among species within the Culicoides variipennis complex, C. sonorensis is considered the primary vector of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in North America. Morphological identification of C. sonorensis within the C. variipennis complex is laborious, time-consuming, and requires entomology expertise. Therefore, in this study we developed and validated a multiplex real-time PCR for rapid detection and differentiation of C. sonorensis from the two other main cryptic species (C. variipennis and C. occidentalis) within the C. variipennis complex. The assay targets the EF1α gene and has a built-in internal control targeting 18 S. The specificity and the sensitivity of the multiplex real-time PCR were evaluated using morphologically identified reference and field-collected specimens. The multiplex PCR was 100% specific when nucleic acid extracted from C. variipennis, sonorensis, and occidentalis specimens was tested. When nucleic acid extracted from pools of midges was tested, the multiplex PCR was able to detect all three Culicoides species with comparable sensitivity. The multiplex assay, however, failed to detect eight morphologically identified C. sonorensis specimens collected from Alberta in 2014. The EF1α gene sequences of these specimens formed a distinct phylogenetic cluster, amongst those from C. variipennis, sonorensis, and occidentalis, suggesting that they belong to a different species. We hypothesize that those specimens might be C. albertensis, the only other species remaining in the C. variipennis complex with known geographical distribution in North America. We believe that this highly sensitive and specific multiplex real-time PCR assay could be an effective tool for rapid detection and differentiation of C. sonorensis, the known vector of BTV and EHDV, in trap collections in future vector surveillance programs.

Abstract The ability of 18F-FDG positron emission tomography (PET) to track disease activity and treatment response in patients with Ankylosing Spondylitis (AS) or Psoriatic Arthritis (PsA) remains unclear. Here, we assessed whether 18F-FDG uptake is a marker of disease activity and treatment response in AS or PsA, and explored the ability of 18F-FDG to predict treatment response. Patients with AS (n = 16) or PsA (n = 8) who were scheduled to initiate treatment with biologics were recruited. Participants underwent a clinical evaluation and an 18F-FDG scan prior to therapy initiation. Eleven participants underwent a follow-up 18F-FDG scan 3 months post-treatment. Images were quantified using a composite measure that describes the inflammatory status of the patient. Clinically involved joints/entheses had higher 18F-FDG uptake compared to unaffected areas (median difference > 0.6, p 8.5, p

Abstract Macroalgae are vital reservoirs for essential epibiotic microorganisms. Among these are growth-promoting bacteria that support the growth and healthy development of their host macroalgae, and these macroalgae can be utilized in agriculture as biostimulants, offering an alternative to traditional agrochemicals. However, to date, no comparative studies have been conducted on the functional profile and bacterial diversity associated with coastal macroalgae of Peru. In this study, we employed amplicon sequencing of the V3-V4 region of 16S rRNA gene in twelve host macroalgae collected from two rocky shores in central Peru to compare their bacterial communities. The results revealed high bacterial diversity across both sites, but differences in microbial composition were noted. The phyla Bacteroidota and Pseudomonadota were predominant. The functional prediction highlighted 44 significant metabolic pathways associated with the bacterial microbiota when comparing host macroalgae. These active pathways are related to metabolism and genetic and cellular information processing. No direct association was detected between the macroalgal genera and the associated microbiota, suggesting that the bacterial community is largely influenced by their genetic functions than the taxonomic composition of their hosts. Furthermore, some species of Chlorophyta and Rhodophyta were observed to host growth-promoting bacteria, such as Maribacter sp. and Sulfitobacter sp.

Abstract Introduction Although obesity substantially influences public health owing to related comorbidities, it has been discovered that the incidence of such issues is not directly related to obesity but to the patient’s unhealthy metabolic status (MUS) independent of the body mass index (BMI). Objectives To describe the prevalence of UMS overall and according to BMI and determine the factors associated with it. Methods A cross-sectional analytical study was used based on the analysis of secondary databases called the Life Stage Nutritional Surveillance Survey (VIANEV). Participants were selected in two stages, finally obtaining 885 participants. UMS was defined based on the criteria of the Adult Treatment Panel III used to define metabolic status in a set of 5 parameters, if the subject presented two or more alterations it was considered UMS. Six groups were formed according to BMI: metabolically healthy, average weight (MHNW) and unhealthy (MUNW), metabolically healthy, overweight (MHOW) and unhealthy (MUOW), metabolically healthy, obese (MHO) and unhealthy (MUO).). Results The total prevalence of UMS was 73.11%, with MUNW, MUOW, and MUO being 47.90%, 80.34%, and 96.44%, respectively. Only 5.31% did not present any metabolic alteration. The multivariable analysis found variations globally according to sex, age, marital status, geographical region, smoking habit, and altitude. Conclusions A high prevalence of UMS was observed in Peru, indicating that BMI alone is not a sufficient indicator of metabolic status. These findings suggest that strategies should be prioritized to address the growing problem of UMS, considering the particularities of each subpopulation and using a multifaceted approach that addresses modifiable and non-modifiable risk factors.

Heritage bridges constitute an integral feature of the urban landscape in numerous cities. However, it is common for these structures to surpass their life cycle, rendering them ill-equipped to withstand the dynamic demands of users and extreme events, particularly hydrological occurrences. This research presents a methodology for the assessment of heritage riverine bridges, with a focus on the Grau Bridge in Peru as a case study. The investigation commences with an exhaustive literature review, complemented by a historical examination, followed by a preliminary diagnosis. Subsequently, hydrological and hydraulic studies are presented, encompassing drone surveys of the riverbed and the bridge, soil analyses, and the application of 1D and 2D models in HEC-RAS. The outcomes of this comprehensive analysis reveal the high vulnerability of the Grau Bridge. Finally, strategic interventions for its conservation are recommended.

El estudio se enfoca en el desarrollo de un micro generador hidroeléctrico mediante el empleo de una turbina de flujo cruzado de admisión inferior que aprovecha el potencial hidráulico de los canales de riego. La metodología utilizada es cuantitativa y experimental, y se basa en un diagrama de flujo que describe las etapas desde la creación hasta la implementación del prototipo. Se tomaron en cuenta variables como la altura y el caudal del agua, así como las dimensiones del canal de riego, las cuales no podían modificarse debido a las actividades agrícolas que se llevan a cabo en el lugar. Los resultados obtenidos de la implementación en campo del prototipo diseñado demuestran que el generador tiene la capacidad de alcanzar un valor de potencia de 93 W con un porcentaje de eficiencia del 11.3% el mismo que produce energía de manera sostenible en áreas remotas. En conclusión, el estudio resalta la necesidad de explorar nuevas fuentes de energía renovales y sostenibles en respuesta a la crisis energética actual que afecta a la sociedad; se enfatiza la imprescindibilidad por adoptar alternativas energéticas más limpias que limite la dependencia por los recursos no renovables a la par de controlar los efectos negativos del cambio climático.

We have investigated the equilibrium properties and rapid-reaction kinetics of the isolated butyryl-CoA dehydrogenase (bcd) component of the electron-bifurcating crotonyl-CoA-dependent NADH:ferredoxin oxidoreductase (EtfAB-bcd) from Megasphaera elsdenii. We find that a neutral FADH• semiquinone accumulates transiently during both reduction with sodium dithionite and with NADH in the presence of catalytic concentrations of EtfAB. In both cases full reduction of bcd to the hydroquinone is eventually observed, but the accumulation of FADH• indicates that a substantial portion of reduction occurs in sequential one-electron processes rather than a single two-electron event. In rapid-reaction experiments following the reaction of reduced bcd with crotonyl-CoA and oxidized bcd with butyryl-CoA, long-wavelength-absorbing intermediates are observed that are assigned to bcdred:crotonyl-CoA and bcdox:butyryl-CoA charge-transfer complexes, demonstrating their kinetic competence in the course of the reaction. In the presence of crotonyl-CoA there is an accumulation of semiquinone that is unequivocally the anionic FAD•- rather than the neutral FADH• seen in the absence of substrate, indicating that binding of substrate/product results in ionization of the bcd semiquinone. In addition to fully characterizing the rapid-reaction kinetics of both the oxidative and reductive half-reactions, our results demonstrate that one-electron processes play an important role in the reduction of bcd in EtfAB-bcd.

Course-based undergraduate research experiences (CUREs) can be invaluable for students, but implementing them across a university requires substantial faculty support. This case study explores the incorporation of CURE elements (discovery, iteration, scientific practices, broad scientific relevance, and collaboration) into the design of a faculty professional learning program intended to support CURE implementation. The findings suggest that incorporating CURE elements into a CURE Faculty Fellows Program can support the simultaneous development and successful implementation of scaffolded CUREs across multiple university departments. This program had an additional, unexpected benefit: it created opportunities for faculty from many disciplines to engage in educational research, augmenting their research and publishing agendas. This article summarizes the program, presents findings, and discusses implications for practitioners and researchers interested in pedagogical reform.

IntroductionHormones produced by the hypothalamic–pituitary–adrenal-gonadal (HPAG) axis are crucial for modulating central nervous system (CNS) function and development throughout a person’s life. Disruptions in HPAG function can impact psychological development, particularly during adolescence—a period marked by psychological growth and the maturation of the HPAG axis. An early indicator of HPAG alterations is ovulatory dysfunction (OD), a common condition among adolescents.MethodsThis study explored the associations between neuroactive hormones and personal growth in adolescents with OD. Female participants aged 12–25 years with OD were recruited, and assessments were conducted to profile their basic hormonal levels and various dimensions of individual development, including self-concept clarity, sense of coherence, self-esteem, perfectionism, self-control, and mood states.ResultsAdolescents with OD (n = 117) had lower self-concept clarity and self-esteem compared to reference data. A significant portion of the sample displayed elevated levels of tension (71.25%), confusion (62.5%), fatigue (58.22%), and depression (52.6%). Self-esteem scores were negatively correlated with DHEAS (r = −0.224; p = 0.026) and glucose (r = −0.249; p = 0.010). Higher levels of free testosterone were associated with increased depression scores (coef = 0.2398; p = 0.002), whereas higher estradiol levels were linked to lower aggressiveness scores (coef = −0.0648; p = 0.001).DiscussionThese findings indicate that hormonal imbalances in adolescents with OD could affect personal growth. Further research is needed to establish causal relationships between the variables considered.

Cuticular waxes coating leaf surfaces can help plants tolerate drought events by reducing non-stomatal water loss. Despite their role in drought tolerance, little is known about how cuticular wax composition has changed during breeding in Canadian bread wheat (Triticum aestivum L.) varieties. To fill in this gap, flag leaves of the Canadian Heritage Bread Wheat Panel, which include 30 varieties released between 1842 and 2018, were surveyed to determine if and how cuticular wax composition in wheat has changed at two breeding ecozones over this period. Following this, a subset of varieties was subjected to drought conditions to compare their responses. As expected, modern varieties outperformed old varieties with a significantly larger head length and reaching maturity earlier. Yet, when challenged with drought, old varieties were able to significantly increase the accumulation of β-diketones to a higher extent than modern varieties. Furthermore, RNAseq was performed on the flag leaf of four modern varieties to identify potential markers that could be used for selection of higher accumulation of cuticular waxes. This analysis revealed that the W1 locus is a good candidate for selecting higher accumulation of β-diketones. These findings indicate that the variation in cuticular waxes upon drought could be further incorporated in breeding of future bread wheat varieties.

ABSTRACT The rapidly developing field of oncolytic virus (OV) therapy necessitates the development of new and improved analytical approaches for the characterization of the virus during production and development. Accurate monitoring and absolute quantification of viral proteins are crucial for OV product characterization and can facilitate the understanding of infection, immunogenicity, and development stages of viral replication. Targeted mass spectrometry methods like multiple reaction monitoring (MRM) offer a robust way to directly detect and quantify specific targeted proteins represented by surrogate peptides. We have leveraged the power of MRM by combining ultra-high performance liquid chromatography (UPLC) with a Sciex 6500 triple-stage quadrupole mass spectrometer to develop an assay that accurately and absolutely quantifies the structural proteins of a pseudotyped vesicular stomatitis virus (VSV) intended for use as a new biotherapeutic (designated hereafter as VSV-GP to differentiate it from native VSV). The new UPLC-MRM method provides absolute quantification with the use of heavy-labeled reference standard surrogate peptides. When added in known exact amounts to standards and samples, the reference standards normalize and account for any small perturbations during sample preparation and/or instrument performance, resulting in accurate and precise quantification. Because of the multiplexed nature of MRM, all targeted proteins are quantified at the same time. The optimized assay has been enhanced to quantify the ratios of the processed GP1 and GP2 proteins while simultaneously measuring any remaining or unprocessed form of the envelope protein GP complex (GPC; full-length GPC).IMPORTANCEThe development of oncolytic viral therapy has gained considerable momentum in recent years. Vesicular stomatitis virus glycoprotein (VSV-GP) is a new biotherapeutic emerging in the oncolytic viral therapy platform. Novel analytical assays that can accurately and precisely quantify the viral proteins are a necessity for the successful development of viral vector as a biotherapeutic. We developed an ultra-high performance liquid chromatography multiple reaction monitoring-based assay to quantify the absolute concentrations of the different structural proteins of VSV-GP. The complete processing of GP complex (GPC) is a prerequisite for the infectivity of the virus. The assay extends the potential for quantifying full-length GPC, which provides an understanding of the processing of GPC (along with the quantification of GP1 and GP2 separately). We used this assay in tracking GPC processing in HEK-293-F production cell lines infected with VSV-GP.

Este artículo es el resultado de un estudio observacional descriptivo realizado en el portal de revistas de la Universidad de Panamá, en el cual se buscaba determinar el nivel de productividad científica en matemática. Después de revisar cada una de las revistas con que cuenta este portal, se determinó que solo en trece de ellas son aceptados artículos relacionados con matemática, ya que las demás son de áreas específicas del conocimiento. En esas trece revistas durante los últimos cinco años (2018-2022), únicamente se han publicado veintiséis artículos de matemática, lo que representa solo un 2,89% de la producción total; este porcentaje incluye artículos que son resultado de alguna investigación y escritos referentes a una revisión bibliográfica.