Search

Initial objectives and design of the Benchmark campaign organized within the European COST Action ES1206 (2013–2017) are described in the paper. This campaign has aimed to support the development and validation of advanced Global Navigation Satellite System (GNSS) tropospheric products, in particular high-resolution and ultra-fast zenith total delays (ZTDs) and tropospheric gradients derived from a dense permanent network. A complex data set was collected for the 8-week period when several extreme heavy precipitation episodes occurred in central Europe which caused severe river floods in this area. An initial processing of data sets from GNSS products and numerical weather models (NWMs) provided independently estimated reference parameters – zenith tropospheric delays and tropospheric horizontal gradients. Their provision gave an overview about the product similarities and complementarities, and thus a potential for improvements of a synergy in their optimal exploitations in future. Reference GNSS and NWM results were intercompared and visually analysed using animated maps. ZTDs from two reference GNSS solutions compared to global ERA-Interim reanalysis resulted in accuracy at the 10 mm level in terms of the root mean square (rms) with a negligible overall bias, comparisons to Global Forecast System (GFS) forecasts showed accuracy at the 12 mm level with the overall bias of −5 mm and, finally, comparisons to mesoscale ALADIN-CZ forecast resulted in accuracy at the 8 mm level with a negligible total bias. The comparison of horizontal tropospheric gradients from GNSS and NWM data demonstrated a very good agreement among independent solutions with negligible biases and an accuracy of about 0.5 mm. Visual comparisons of maps of zenith wet delays and tropospheric horizontal gradients showed very promising results for future exploitations of advanced GNSS tropospheric products in meteorological applications, such as severe weather event monitoring and weather nowcasting. The GNSS products revealed a capability of providing more detailed structures in atmosphere than the state-of-the-art numerical weather models are able to capture. In an initial study on the contribution of hydrometeors (e.g. cloud water, ice or snow) to GNSS signal delays during severe weather, the effect reached up to 17 mm, and it was suggested that hydrometeors should be carefully accounted for within the functional model. The reference products will be further exploited in various specific studies using the Benchmark data set. It is thus going to play a key role in these highly interdisciplinary developments towards better mutual benefits from advanced GNSS and meteorological products.

Increasing climate change has led to an increase in urban flood events. Events with a return period of twenty years become events with a period of two to three years. The primary objectives of this study are to evaluate the performance of flood regulation models using different input data and compare their performance for flood regulation supply (FRS) assessment. The assessment of the ecosystem services of urban FRS was performed using two models: Urban Flood Risk Mitigation (UFRM) by Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) and IMECOGIP Waterflow Regulation (Retention Model) (WRRM). They rely on relatively accessible open-source data, require a short time to model each scenario and provide opportunities to interpret results in different spatial and measurement units. We performed several improvements to the input data, including updating the national ecosystem-type assessment for the case study area by connecting it with the Urban Atlas typology and proposing an approach for soil data refinement. The outputs of the two models show significant differences in FRS values for each land-use/land-cover (LULC) class. The UFRM assesses different impervious urban classes with different values, varying from very low to medium. In contrast, the WRRM assesses the FRS in densely sealed areas with one fixed value for low supply (where nearly all rainfall is transformed into runoff).

COVID-19 presents with respiratory symptoms and signs in the majority of cases. The central nervous system can be affected directly or indirectly, which is demonstrated by a number of neurological abnormalities and complications. The aim of this study is to present a clinical case of SARS-CoV-2-associated encephalitis and highlight the severity of this neurological illness over the course of COVID-19. Etiological diagnosis methods include examination of the nasopharyngeal swab and cerebrospinal fluid by PCR. A 45-year-old man with fever, altered consciousness, and tonic-clonic seizures was admitted to the Clinic of Infectious Diseases, St George University Hospital, Plovdiv. The diagnosis of COVID-19 encephalitis is based on typical clinical and laboratory data, positive SARS-CoV-2 PCR, and exclusion of other likely causative agents into the cerebrospinal fluid. The patient was discharged two weeks after admission without neurological sequelae. The case confirms the clinical variety of COVID-19 and the possibility of severe neurological manifestations. Encephalitis is a rare but potentially life-threatening condition. Physicians should be aware of encephalitis in SARS-CoV-2 patients with neurological symptoms.

Animal welfare is a daily concern for livestock farmers. It is known that the activity of cows characterizes their general physiological state and deviations from the normal parameters could be an indicator of different kinds of diseases and conditions. This pilot study investigated the application of machine learning for identifying the behavioral activity of cows using a collar-mounted gyroscope sensor and compared the results with the classical accelerometer approach. The sensor data were classified into three categories, describing the behavior of the animals: “standing and eating”, “standing and ruminating”, and “laying and ruminating”. Four classification algorithms were considered—random forest ensemble (RFE), decision trees (DT), support vector machines (SVM), and naïve Bayes (NB). The training relied on manually classified data with a total duration of 6 h, which were grouped into 1s, 3s, and 5s piles. The obtained results showed that the RFE and DT algorithms performed the best. When using the accelerometer data, the obtained overall accuracy reached 88%; and when using the gyroscope data, the obtained overall accuracy reached 99%. To the best of our knowledge, no other authors have previously reported such results with a gyroscope sensor, which is the main novelty of this study.

West Nile virus (WNV) is transmitted via mosquito bites and causes ubiquitous zoonosis. Most infections in humans are asymptomatic, approximately 20% present as fever, and less than 1% as neuroinvasive disease. Central nervous system involvement is presented as meningitis, encephalitis, acute flaccid paralysis, or a combination of them. West Nile neuroinvasive disease (WNND) has a severe clinical course, potentially fatal outcome and frequent neurological sequelae in survivors. Risk factors for neurological impairment are advanced age and immunosuppression. Here we present a clinical case of meningoencephalitis caused by WNV and a brief literature review. Clinical and epidemiological data, laboratory, microbiological, molecular methods and imaging techniques were used. The cytokine levels of IL-6, IL-8, IL-10, IL-12(p40) and TNF-α in cerebrospinal fluid (CSF) and serum were also measured. We present a 55-year-old man with a sudden onset of headache, vomiting and fever. The symptoms appeared after a recent trip to Türkiye and involved multiple mosquito bites. Neck stiffness, disturbances in consciousness, signs of cerebral edema and subsequently focal neurological deficits were observed. The etiological diagnosis was verified by positive polymerase chain reaction for WNV and the presence of specific IgM antibodies in the CSF. After a 28-day hospital stay, the patient was discharged and referred to a Physiotherapy Unit due to residual motor deficits. WNV etiology should be suspected in patients with clinical and laboratory signs of viral neuroinfection, mosquito bites, and/or travel to endemic regions.

Lavender essential oil is an economically important ingredient in perfumery, the food industry, and pharmacy. There is notable diversity in the composition of lavender essential oils. The reasons are the high genetic diversity of lavender cultivars and the variety of ecological specifics in the regions of cultivation. The aim of this research is to check which lavender essential oil variety is best regarding the content of the most important components through comparative statistical tests. We created a data set of 88 lavender essential oil samples from 16 countries. The multivariate statistics (hierarchical and non-hierarchical clustering) and factor analysis reveal hidden relationships between the objects of the study (samples) or between the variables characterizing the objects (chemical descriptors–16 components). The results are discussed in detail. All samples from Bulgaria, together with a few of the Italian, French, Greek, Indian, and Chinese samples, fall into one cluster with the standard maximums. Graphical abstract:

The present study aimed to determine the influence of rearing method on behaviour and welfare in Assaf lambs during the first two weeks of the neonatal period, in an intensive rearing system. The experiment was conducted with 24 Assaf lambs, in which two methods of management were applied – natural rearing (NR) and artificial rearing (AR). Forced separation of lambs from their mothers caused 74% fewer suckling attempts during the first three days of the neonatal period, which reduced suckling time by 57%. The lambs reared under NR, spent more time moving and less time standing and lying. Overall, it was found that the type of rearing affects the suckling time. Artificial rearing decreased lying and playing time, which is indicative of the improved welfare of NR lambs. The two rearing methods (NR and AR) did not affect blood total protein or albumin, indicating no significant effect of different rearing methods on these haematological variables. Artificial rearing does not disturb the welfare and behaviour of the animals when it is done correctly and stress appears transient and animals quickly adapt with similar growth rates.

The purpose of this study is to outline some numbers and facts about the level of sustainability of the consumption of Varna households. The results of two surveys conducted on the implementation of a project funded by the Bulgarian National Science Fund "Sustainable Consumption in the Urban Environment: Regional Differences" are used. The collection of primary data is realized through the method of personal interview. The surveys were conducted in two waves – the first wave in the period February – April 2021, and the second – in the period October – December 2022. In total, representatives of 729 Varna households were interviewed – 344 in the first wave of surveys and 385 – in the second. The results of the survey are useful for checking the sustainability of the consumption of other households (other cities) and other periods.

Ecosystems provide various goods and services to society and their valuation is among the main objectives of the concept of ecosystem services (ES). The mapping of ecosystems is the main building block of the whole process of the Mapping and Assessment of Ecosystems and their Services (MAES). The analyses of the ecosystem data produced during the implementation of the national methodology for mapping ecosystems in Bulgaria (MAES BG) reveal some problems that may cause confusion in cases of integrated assessment of all ecosystem types. In this paper, we present an approach that enables formulation of a uniform spatial dataset based on the mapping of the main ecosystem types, that can be used for mapping of ES at a river basin scale. It has been applied to the upper part of the Ogosta River basin and the result is a topologically correct uniform spatial data layer. The approach gives one possible solution to problems related to the different sources of information and the discrepancies between ecosystem types in the national mapping of ecosystems in Bulgaria. It is based on the use of a uniform spatial framework that outlines the ecosystem types and sets the initial database for further mapping. This ensures a topologically correct spatial dataset for the ecosystems and a background for further updates for each ecosystem at the different levels of MAES typology. The most appropriate spatial basis for the territory of Bulgaria is the database for the physical blocks of the Ministry of Interior. Its application to the studied river basin gives encouraging results and can be used as an example for similar areas. Further development of the approach will ensure the mapping of the forest ecosystems at level 3 of the MAES BG typology and more precise delineation of the grassland, heathland, freshwater, and sparsely vegetated ecosystems.

Habitat maintenance as an ecosystem service (ES) is essential for the protection of natural capital, however, it is among the most challenging services for definition and evaluation. The present study is focused on assessing and mapping habitat maintenance ecosystem service in Rila and Pirin Mountains in Bulgaria for strategic planning purposes by better understanding the link between the potential of providing this ecosystem service and biodiversity in five protected areas (PAs). An integrated approach for the assessment of the condition of ecosystems in PAs and their potential to provide ecosystem services was applied and further developed in the present study. The results showed that the conservation regime allowed the territories to preserve a high degree of naturalness in a very good ecological condition – 96477 ha (73%) of the total case-study area, and 33078 ha (77%) of the target PAs, respectively. The potential of ecosystems to provide habitat maintenance ES is high to very high for 84% of the total studied area (81258.9 ha) and for 96.4% (31906 ha) of the area of the target PAs. A current assessment and mapping show the role of protected areas as spatial natural capital assets that purposefully and actively support their prioritized habitat maintenance functions as spatial guarantors for the sustenance of rich packages of material, regulating, and cultural functions in significant geographic areas. The results demonstrate the importance of protected site management in mountain areas in ensuring sustainable cooperation and consumption of ecosystem services in peripheral mountain communities of the European Union highly dependent on available natural capital.

During recent four decades, the world has faced two horrific, deadly pandemics. In the background of the ongoing pandemic HIV/AIDS, the people were affected with the newly emerging Corona virus SARS-CoV-2, that rapidly turned into pandemic dimensions. There are data that, in various aspects, HIV infection was similar to SARS-CoV-2. The purpose of this review was to search for similarities and differences between both pandemics in view to use the experience of the past and knowledge we have acquired in order to better overcome current and future pandemics. Material/Methods: The iterature used is based on databases PubMed, Embase and random search on the Internet with the keywords COVID-19 and HIV, as well the Boolean operator "AND" to achieve a search of "COVID-19 and HIV". Review Results: In the present review summary, we provide data for similarities and differences regarding virological, immunologic, clinical and pharmacological aspects of HIV and SARS-CoV-2 infections and associated with them pandemics. Conclusion: SARS-CoV-2 was here and will remain here. COVID-19 is not the last pandemic, and people should be prepared for new encounters with agents of high virulence and pandemic potential.

Streptococcus equi subsp. zooepidemicus is a rare etiologic agent of bacterial meningitis in humans. The disease is a zoonotic infection and is transmitted through close contact with domestic animals, mainly horses. Only 37 cases of Streptococcus zooepidemicus meningitis have been reported in the literature until July 2023. The aim of this study is to present a rare clinical case of S. zooepidemicus-related meningitis in a human immunodeficiency virus (HIV)-positive patient and analyze the literature. We present a 23-year-old horse breeder patient with advanced immunosuppression due to acquired immunodeficiency syndrome (AIDS) and S. zooepidemicus meningitis, admitted at the Clinic of Infectious Diseases, St. George University Hospital, Plovdiv. The course of meningitis was severe since the beginning, with significant cerebral edema, disturbances in consciousness, persistent fever, and the development of complications against the background of AIDS-related conditions. S. zooepidemicus was microbiologically detected from cerebrospinal fluid culture. After prolonged treatment and a long hospital stay, the patient’s condition improved, and eventually he was discharged and recovered from the acute neuroinfection. Although extremely rare, S. zooepidemicus should be considered in patients with clinical and laboratory evidence of bacterial meningitis who have contact with animals, especially horses, other domestic animals, and their dairy products, as well as in immunocompromised patients. To the best of our knowledge, the current clinical case is the first report of S. zooepidemicus-related meningitis in a patient with HIV/AIDS.

Mediterranean countries are a cauldron of cultural exchange, with a strong emphasis on wild plants in cuisine traditions. Many of these plants belong to the family Apiaceae. The common climate determines the common range of distribution. While many plants have wide distribution, the range of distribution of others is restricted to Western Mediterranean or North Africa. This review investigates wild plants from the family Apiaceae traditionally used as food in 13 study sites—11 countries in the Mediterranean and adjacent territories—the mainland and 3 islands. The aim is to trace patterns of native distribution versus consumption. As a result, 81 wild umbellifers are listed, traditionally used as food. Their consumption and distribution patterns are described and discussed. Interestingly in 8 of the 13 study sites (61.5%) are recorded 50 plant taxa (66.7% of all wild umbellifers, traditionally used as food) which are consumed in only one particular country. These are as follows: 8 taxa in Morocco, 9 taxa in Spain, 2 taxa in Sicily, 3 taxa in Bulgaria 3 taxa in Crete, 8 taxa in Armenia, 14 taxa in Turkey, and 3 taxa in Jordan. However, these 50 restrictedly consumed plants are distributed in more than one country (except 15 taxa, which are endemics). Many of the plants that are used in certain countries are not consumed by the neighboring people. The results of the two statistical tests, namely Jaccard index and heatmap clustering (double dendrogram), are discussed. The presence of an outlier, such as Bulgaria, which shares borders, history, and culture with Greece and Turkey, emphasizes the importance of local climate for plant distribution and consumption over cultural interactions. The same was observed for some pairs of countries, such as Spain and Morrocco and Turkey and Armenia, although they had the highest number of common plants that are both distributed and consumed as food.

IntroductionEssential tremor (ET) is characterised by postural and intentional tremor typically affecting the upper limbs, which can negatively impact functionality and quality of life. Magnetic Resonance-guided Focused Ultrasound (MRgFUS) is a novel and promising non-invasive treatment for ET which offers instantaneous results.MethodsUsing interpretative phenomenological analysis we explored the experience of undergoing MRgFUS in six ET patients as well as their experiences pre- and post-procedure.ResultsOne-time, retrospective semi-structured interviews were conducted and six themes emerged: Life pre-treatment: “It’s everyday tasks that get you down” and “Most people who understand, they are okay. Some people aren’t”; MRgFUS: Treatment day: “Going into the unknown” and “There’s no way I was going to press that button”; and Life post-treatment: “One is good. Two is better” and “Am I fixed, am I better now?.”DiscussionThe findings point to a significant period of adjustment associated with living with ET and the effects of undergoing ET MRgFUS treatment. As ET progressed, participants struggled to cope with increasing symptoms and had to develop coping strategies to manage life with ET. The procedure itself was perceived as strange and extraordinary and despite some immediate adverse effects participants were determined to go through with it. Post procedure, all participants reported tremor suppression which was life changing. While some participants still felt burdened by ET, others expressed it took them a while to psychologically adjust to what essentially was their new body. This study has highlighted the need for patients to be supported at all stages of their ET journey.

Background. The world continues to struggle with the 2019 coronavirus disease (COVID-19). The pandemic is under control, but the disease exists and it is extremely important to have algorithms for early diagnostic and prognostic guidance. The aim of the study is to find correlations between the spectrum of clinical symptoms with the disease severity and the outcome of COVID-19, aiming to maximally early diagnosis and establishing early predictors for severity and fatal outcome. Materials and methods. The study included 169 adults hospitalized at the University Hospital St. George, Plovdiv, between September 2021 and December 2022 with a PCR verified diagnosis of COVID-19. The methods of clinical analysis (history and clinical examination) and assessment of oxygen saturation were used. For the purposes of the study, patients were distributed into groups according to age (below and over 60 years); disease severity (moderate or severe/critical clinical course), and outcome (survived or died). Results. According to analysed data, 92 patients (54.43%) were men, and 69 (40.82%) were under 60 years; 126 patients (74.5%) had a moderate and 43 (25.4%)- severe clinical course. The disease has a gradual onset in 149 (88.1 %). The most frequent initial symptoms were fever and fatigue (60.4 %), followed by fatigue with arthro-myalgias (26.2%). Co-morbidities were documented for 140 patients (82.8%). COVID-19 was severe/critical in 14.3% of patients under 60 years and 34.1% of patients ≥ 60 years (p < 0.01), with case fatality rate 7.4% vs. 25% respectively (p < 0.001). Conclusion. Our data highlight the importance of advanced age (over 60 years) and comorbidities ( arterial hypertension, diabetes mellitus, cirrhosis hepatis) as high-risk factors for severe course and fatal outcome of COVID-19

Lavender essential oil is economically important and widely used in aromatherapy perfumery, food industry and pharmacy. Bulgaria is a global leader in lavender cultivation overtaking countries such as France, UK, China, India, and Spain during the last few years. The aim of this research is: 1) to characterize a lavender essential oil sample obtained from agricultural plantation near Pomorie, Bulgaria; 2) to perform descriptive statistical test based on a data set of 13 samples available in 4 publications, 3) to compare the varieties regarding the quantity of the most important components such as linalool and linalyl acetate 4) to summarize the pharmacological effects of the main components. As a result of GS/MS analysis of the essential oil sample obtained from agricultural plantation near Pomorie, we identified 44 compounds. The major constituents were linalyl acetate (27.5%) linalool (24.1%), E-β-ocimene (7.0%), terpinen-4-ol (5.1%) caryophyllene (4.5%), carvacrol (4.4%), lavandulyl acetate (3.5%), Z-β-farnesene (3.3%), and -Z-β-ocimene (3.2%). Linalool and linalyl acetate are the main ingredients based on which the quality of the essential oil is evaluated. In the studied samples they fluctuated between varieties depending on the year of extraction and the locations of origin in Bulgaria. Some varieties were characterized by a more stable ratio of linalool – linalyl acetate, compared to others. The main other components of our sample as well as the other examined Bulgarian samples fit the standards according to the requirements of ISO (2002) and the of European Pharmacopoeia (10th edn., Council of Europe 2020) with few exceptions. Lavender oil has numerous pharmacological applications based on its anxiolytic, sedative, antioxidant, anti-inflammatory, antitumor and antimicrobial activities. Although linalool and linalyl acetate largely contribute to these effects, the overall efficacy of lavender oil is proven to be due synergistic relationships between the components.

Introduction. Bacterial meningitis is a severe disease with high mortality and frequent residual neurological sequelae. It is associated with multiple risk factors. The aim of the study was to establish the main risk factors associated with bacterial meningitis and to outline the incidence of chronic diseases in patients with bacterial meningitis and their relationship to the patients’ age and etiology of meningitis, if any.

Introduction: The internal thoracic artery (ITA) has proven to be the best graft for surgical myocardial revascularization, especially in the configuration left ITA (LITA) to the anterior descending branch of left coronary artery (LAD). Its harvesting is usually accompanied by pleurotomy, followed by drainage tube placement into the pleural cavity, using the so called intrapleural method. Extrapleural technique for LITA harvesting is also practiced in many cardiac surgery centers and with this technique the pleura is left intact. Aim: The aim of our study was to investigate the impact of both techniques of LITA harvesting on lung function and incidence of postoperative complications early after operation. Materials and methods: We analyzed retrospectively data of 82 operated patients scheduled for surgical myocardial revascularization and meeting certain inclusion and exclusion criteria. The patients were divided into two groups depending on the way LITA was prepared. Lung parameters and incidence of surgical complications were registered in the early postoperative period until day 30. Results: The patients from both groups had similar preoperative characteristics and risk factors. Those with preserved pleura showed significantly better results of the studied pulmonary parameters and lower complications event rate during early postoperative follow-up. Conclusions: Maintaining the pleura integrity during LITA harvesting is beneficial for lung function and reduces the rate of complications in the early postoperative period.

The purpose of this article is to present the process for developing individual indexes of sustainable household actions in several thematic areas of sustainable consumption. These areas are energy efficiency, electricity and water consumption, food consumption, transport use and product disposal. Based on these individual indexes, a proposal for the creation of an aggregated individual household index for sustainable consumption is discussed. Based on the developed indexes, a segmentation of households into three basic segments is proposed. The proposed indexes are designed based on the results of a survey conducted among 342 households from the city of Varna, Bulgaria in the months of January - April 2022. The proposed indexes, after adaptation, can be used as a tool for self-assessment of sustainable household consumption in different cities and countries. In this sense, as a result of their implementation, it is expected that the motivation of households towards inclusion in sustainable consumption will be increased.

The aim of the present study was to identify the adaptability of Normandy cows raised in a loose system to the climatic and technological conditions and to compare their adaptability with already acclimatized Simmental cows in one farm in Southwestern Bulgaria. Total of 20 cows at same age (II – IV lactation) and same body weight (630-660 kg) from both breeds were classified into 2 groups (each having 10 cows) and subjected to skin thickness measurement, fibers total weight determination and fiber categorization during the winter and summer season. The results have shown that during the winter season, the Normandy breed had significantly higher skin thickness at the elbow compared to the Simmental breed, whereas no differences were observed in the skin thickness between both breeds either at the neck or at the middle of the last rib. During the summer season, the differences on the skin thickness compared to the Simmental breed were observed at the neck (p0.05). During the winter seasons, the coat of Normandy cows contained more soft fibers in comparison to the Simmentals cows. Furthermore, the changes in the observed parameters, influenced by climatic conditions showed similar pattern in both breeds. It can be concluded that the two breeds have emphasized their genetic potential and have a good adaptability to the temperate continental climate of the region.

There is a high growing demand for regulating ecosystem services such as air quality regulation, regulation of air temperature and humidity, and flood regulation, in urban ecosystems which is important for urban planning. A comprehensive review of the current studies of the urban ecosystem, regulating ecosystem services, and their connection with urban planning actions is needed. The current paper presents such a review conducted in six stages to evaluate the state-of-the-art of regulating ecosystem services and their relationship with urban planning. It includes 58 papers selected after a precise keywords search and developed by publication screening, defining indicators, developing an assessment template, and meta-analysis of the results. The analyses are focused on spatial data used in the studies, the methods applied for ecosystem services assessment, and the relationships between regulating ecosystem services, urban planning, and green infrastructure. The most studied regulating ecosystem services within reviewed publications are regulation of chemical composition of atmosphere and oceans, regulation of temperature and humidity, including ventilation and transpiration, and hydrological cycle and water flow regulation (including flood control, and coastal protection). Although urban planning-related papers are only 1/3 of the pool of papers, appropriate results have been obtained for assessing the urban planning-regulating ecosystem services relation. The review also identified some significant knowledge gaps that can be used as a starting point for future studies.

Karst is a widely spread natural phenomenon which provides essential benefits to human society, such as drinking water. The water cycle in the karst geosystems is the main factor for their formation and at the same time one of the main drivers for ecosystem services (ES) provision. The monitoring of the water cycle can provide valuable information regarding its functioning and ensure data for ES assessment. This paper aims to present an overview of the monitoring of the water cycle in the karst geosystems and the opportunities to integrate the monitoring data into the water regulation ES assessment. The monitoring of the water cycle is based on the methodological framework ProKARSTerra. It is applied in model karst geosystems, which are representative of the main karst types in Bulgaria. One of them is the Brestnitsa karst geosystem, which is the case study of this work. The monitoring ensures data for analyses of the water cycle which can be used in the assessment of water-related ecosystem services. The results from the analyses of the data requirements and availability show that some services such as water flow regulation and regulation of chemical condition of freshwaters can be easily provided through data for quantification, while for others further studies are needed. The results of the long-term integrated monitoring in Brestnitsa karst geosystem provide the foundation for important conclusions and models for the karst genesis and function under global changes and active anthropogenic pressure. Their integration into the assessment framework and mapping of ecosystem services is an essential step towards the development of models for sustainable use of natural resources in the karst areas.