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BACKGROUND: Neoadjuvant systemic therapy for triple-negative and HER2-positive breast cancer can achieve a complete pathologic response in approximately 60% of patients. Pathological complete response to neoadjuvant systemic therapy is a predictive factor consistent with a positive prognosis which can be accurately determined by percutaneous vacuum aspiration biopsy. AIM: To evaluate the amount of surgery in the group of patients with triple-negative and HER2-positive early breast cancer whose complete pathologic response was confirmed by vacuum aspiration biopsy and who did not undergo standard surgery. MATERIALS AND METHODS: The following single-centre study has been conducted in the Russian Federation. Women aged 18 years old and older with unicentric (cT1-2N0-1M0 by classification TNM) triple-negative or HER2-positive breast cancer with complete clinical response after neoadjuvant systemic therapy according to imaging diagnostic methods. The patients underwent vacuum aspiration biopsy on the mammary gland under ultrusound control with standard amount of surgical intervention on the axillary region. In case when no invasive or in situ disease was detected, no further breast surgery was performed and the patients underwent standard radiation therapy. The survival with no tumor relapse in the ipsilateral mammary gland was assessed as the primary endpoint. The study is registered on сlinicaltrials.gov, NCT04293796. RESULTS: Between June 2020 and May 2022, twenty-seven (84.4%) patients (all women; mean age 47.5 (3268) years) were enrolled in the study and underwent visually controlled vacuum aspiration biopsy. The average initial tumor size was 20.5 (735) mm. 7 (25.9%) patients initially had metastases in the axillary lymph nodes confirmed by puncture biopsy; 15 (55.6%) patients had HER2-positive breast cancer, and 12 (44.4%) had triple-negative breast cancer. A study of vacuum aspiration biopsy samples showed that 6 (22.2%) patients had a residual tumor, and 21 (77.8%) patients achieved complete pathologic response (95% confidence interval 53.2 to 81.4). At a median follow-up of 12.4 months, there were no tumor relapses in the ipsilateral mammary gland in 21 patients with complete pathologic response confirmed by vacuum aspiration biopsy after neoadjuvant systemic therapy, as well as other relapses or progression of the disease. CONCLUSIONS: The patients with a complete pathologic response, which is confirmed by the vacuum aspiration biopsy method, who refused to perform surgery on the mammary gland do not demonstrate worse results with a short period of observation. However, additional prospective clinical studies are needed.

In the article, the author considered the critical aspects of state regulation of the financial market. Various interpretations of the category "state regulation of the financial market", which prevail among representatives of the scientific community, were singled out. The article highlights the factors that determine the need for state regulation of the financial market: it forms the basis for the implementation of a significant set of economic priorities for the country's development; ensuring the stability of the functioning of the financial system by mutual coordination and balancing the interests of all participants, etc. The author also identifies several shortcomings and problematic aspects of state supervision and control, state regulation in general. In particular, one of these problems was determined to be the ineffectiveness of the current ones, as well as the lack of comprehensive legislative acts regarding the methods of state regulation of the financial market. The article presents the main provisions of the legislative framework that regulates the process of providing financial services in Ukraine. The author also emphasised that in the process of state regulation of the financial market, the state can influence not only directly, that is, using direct methods, but also indirectly - using monetary, fiscal, and investment policy tools. The article emphasized that the bodies of state regulation of the financial market can be conditionally divided into two levels. In particular, it is the legislative level of power and the executive level of power. Having comprehensively analyzed the issue of state regulation of the financial market, it was emphasized that Ukraine lacks a single agreed policy on regulating each sector of the financial market. The article determined that the state regulation of the financial market must be considered through the prism of a set of certain methods, means and methods, with the help of which it is directly possible to carry out regulation and to some extent control all participants of the financial market in order to observe and ensure their interests.

In the article, the author considered the critical aspects of state regulation of the financial market. Various interpretations of the category "state regulation of the financial market", which prevail among representatives of the scientific community, were singled out. The article highlights the factors that determine the need for state regulation of the financial market: it forms the basis for the implementation of a significant set of economic priorities for the country's development; ensuring the stability of the functioning of the financial system by mutual coordination and balancing the interests of all participants, etc. The author also identifies several shortcomings and problematic aspects of state supervision and control, state regulation in general. In particular, one of these problems was determined to be the ineffectiveness of the current ones, as well as the lack of comprehensive legislative acts regarding the methods of state regulation of the financial market. The article presents the main provisions of the legislative framework that regulates the process of providing financial services in Ukraine. The author also emphasised that in the process of state regulation of the financial market, the state can influence not only directly, that is, using direct methods, but also indirectly - using monetary, fiscal, and investment policy tools. The article emphasized that the bodies of state regulation of the financial market can be conditionally divided into two levels. In particular, it is the legislative level of power and the executive level of power. Having comprehensively analyzed the issue of state regulation of the financial market, it was emphasized that Ukraine lacks a single agreed policy on regulating each sector of the financial market.

Introduction. Differential diagnosis of some pathological processes in the breast is difficult on the background of dense breast tissue. This often leads to false conclusions and to late diagnosis of breast cancer (BC) or unreasonable biopsy in a benign process. 50% of breast cancers detected less than 12 months after elective mammography were associated with high density of breast tissue. An important advantage of contrast enhanced spectral mammography (CESM) is that it does not depend on the size of the lesions and the X-ray density of the breast tissue. Objective. To compare the diagnostic performance of CESM and digital mammography (DM) in detection of breast cancer in a group of women with dense breast tissue.Materials and methods. The data of 438 patients with suspected breast cancer examined from August 2018 to January 2021 were analyzed in the study. The mean age of women was 50 ± 11 years (from 21 to 86 years). In the study group 154 (35%) malignant and 284 (65%) benign lesions were identified. All lesions were histologically verified. Breast tissue density corresponded to types A and B in 161 patients and corresponded to C and D types in 277 patients according to the ACR classification. 154 cases of breast cancer were identified, including 49 patients with density A and B and 105 patients with density C and D types.Results. Sensitivity, specificity and overall accuracy of DM were 85.7%, 87.3%, 86.8%, respectively. Diagnostic performance of CESM significantly higher than of DM with sensitivity, specificity and accuracy of 96.8% (p < 0.001), 93.3% (p = 0.015), 94.5% (p < 0.001), respectively. CESM had high positive and negative predictive values of 88.7% (p = 0.012) and 98.1% (p < 0.001), which exceeded those of DM – 78.6% and 91.9%, respectively. The diagnostic performance of DM and CESM were comparable in women with normal breast density (types A and B according to ACR), but in patients with high breast density (types C and D according to ACR), CESM was significantly more sensitive in detecting breast cancer.Conclusion. Thus, diagnostic efficiency of CESM in detecting breast cancer significantly higher in comparison with digital mammography.

Breast cancer is one of the most common malignant neoplasms among women nowadays. Timely diagnosis is the basis for effective and successful treatment of this disease. The existing classical methods of X-ray examination (MG, ultrasound, MRI) play a major role in the detection of breast cancer, but in some cases, the diagnosis of breast cancer can be difficult. Therefore, diagnostic performance of scintimammography with specialized gamma camera called molecular breast imaging (MBI) is now of particular clinical interest.Materials and methods. 312 patients with a preliminary clinical diagnosis of breast cancer were examined in the department of radionuclide diagnostics of N.N. Petrov Research Center of Oncology. They underwent scintimammography (MBI) with a specialized gamma camera manufactured by GE (Discovery 750b). Images of both breasts were obtained 15 min after intravenous injection of 500 MBq activity of 99mTc-MIBI. The foci of moderate and intensive radiopharmaceutical accumulation in breast were considered as a sign of a tumor. The diagnostic performance of MBI and the sensitivity in detection of various biological subtypes of cancer were calculated by comparing with the results of morphological and immunohistochemically studies.Results. The sensitivity, specificity and accuracy of the MBI in the diagnostics of breast cancer were 88, 75 and 87%, respectively. Sensitivity of this method in detection of different biological subtypes ranged from 89% to 95% with lowest performance found in luminal A subtype.Conclusion. Scintimammography (MBI) is an informative way of breast cancer diagnostics. The revealed differences in the sensitivity of the method in patients with different biological subtypes of breast cancer confirm the need for further study of this topic.

Background. The accurate and early diagnosis of breast cancer can improve efficacy of the treatment. The standard diagnostic methods such as mammography, ultrasound, and magnetic resonance tomography have a pivotal role in the detection of breast tumors, however, in some cases, they have low diagnostic accuracy. Mammoscintigraphy (MSG) / molecular breast imaging (MBI) with tumor-specific radiopharmacy 99mTc-Technetril in patients with breast cancer can considerably increase the accuracy of diagnosis. However, the diagnostic performance of MSG / MBI in the detection of different biological subtypes of breast cancer is still under investigation.Aim. To evaluate the accuracy of MSG / MBI with 99mTc-Technetril in diagnosis of different biological subtypes of breast cancer.Materials and methods. The analysis included the results of MSG / MBI of 1080 patients (2154 mammary glands), who were examined for suspected breast cancer. MSG / MBI were performed 5–15 min after intravenous injection into the vein of one of the feet of 370–740 MBq of tumor-specific radiopharmacy 99mTc-Technetril. Examinations performed from 2007–2020 was carried out on the emission computed tomography Forte (Philips); since 2020 the molecular visualization has been providing on the special gamma-camera Discovery NM750b (General Electric). The obtained data were evaluated by 2 experienced radiologists. Verification of changes in breasts was provided by morphological examination (1060 cases) or dynamic observation.Results. The sensitivity, specificity and overall accuracy of MSG / MBI were 90 %, 98 %, 95 % correspondingly. When diagnosing tumors with a diameter of up to 10 mm, the sensitivity of MSG / MBI was decreased to 83 %. In patients with various biological subtypes, the sensitivity of MSG / MBI was as follows: luminal A – 88 %; luminal B– – 91 %; luminal B+ – 92 %; triple negative – 93 %; HER2-positive – 96 %. The intensity of tumor uptake depended on the biological subtype of breast cancer. The average values of the 99mTc-Technetril uptake coefficient were as follows: luminal A – 1.59; luminal B– – 1.71; luminal B+ – 1.95; triple negative – 1.93; HER2-positive – 2.22.Conclusion. Retrospective analysis indicate high diagnostic performance of MSG / MBI: sensitivity – 90 %, specificity – 98 %, accuracy – 95 %. There are significant differences in the intensity of 99mTc-Technetril accumulation in tumors in patients with different biological subtypes of breast cancer (p = 0.01–0.004). MSG / MBI characterized by significant differences in the sensitivity in the diagnosis of luminal A and HER2+ breast cancer subtypes: 88 % and 96 %, respectively (p = 0.02).

Background. Achieving a pathologic complete response (pCR) after neoadjuvant systemic therapy (NST) is a predictive factor for improving disease free and overall survival. In triple negative (TN) and HER2-positive breast cancer (BC), the pCR rate exceeds 60 %. Patients with TN and HER2-positive BC who demonstrate an excellent response to NST are like­ly ideal candidates for downsizing surgery. The condition for reducing the volume of surgical intervention is a reliable determination of pathologic complete response using instrumental imaging and biopsy methods.Aim. To further assess the accuracy of post-NST image-guided biopsy to predict pCR.Materials and methods. Sixty one patients with T1-3N0-3 triple negative or HER2-positive BC receiving NST in the Department of Breast Tumors of the NMRC of Oncology named after N.N. Petrov in the period from 2017 to 2019 were enrolled in this single-center retrospective trial. Patients underwent ultrasound-guided core-biopsy of the initial breast tumor region before surgery. Findings were compared with findings on pathologic evaluation of surgical specimens to determine the performance of biopsy in predicting pCR after NST.Results. After neoadjuvant systemic therapy, clinical partial response (cPR) was diagnosed in 47 (77 %) patients, clinical complete response (cCR) in 14 (23 %) patients. pCR in the core-biopsy tissue and surgical material was achieved in 46 (75.4 %) and 37 (60.7 %), respectively. Performance of image-guided core-biopsy: sensitivity 100 % (95 % confldence interval (CI) 90.51-100), specificity 62.5 % (95 % CI 40.59-81.20), false-negative rate (FNR) 0 %, positive-pre­dictive value (PPV) 75.00 % (95 % CI 59.46-85.99), negative predictive value (NPV) 100.00 %.Conclusion. This retrospective trial showed that ultrasound-guided core biopsies are accurate enough to identify breast pCR in patients with triple-negative or HER2-positive BC with good response after NST (FNR 0 %). Based on these results, a prospective clinical trial has commenced in which breast surgery is omitted in patients with a breast pCR after NST according to image-guided biopsy.

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The beef cattle breeding development is one of the strategic directions for the livestock development in Russia and the CIS countries, including the Republic of Belarus. The livestock fattening most important point is minimizing the costs. Studies have shown that the animals’ greatest body weight 900 kg had had adult culled cattle over a period of 120 days, as well as heifers with calving on pasture during the 30-month fattening. During 28-month fattening of suckling steers at pasture housing, the amount of feed energy unit per day turned out to be the smallest among technologies with the largest number of fattening periods, and amounted to 29.3 at animals fattening during five periods of pasture housing versus 39.7 in two periods of intensive grain fattening: 1 day of pasture housing costs 3.5 versus 12.5 feed energy unit in other periods. In young animals raised on suckling, the best average body weight was equal to 720 kg, but weaning calves showed 710 kg. The feed costs per 1 kg of weight gain were equal to 0.04, 0.10 and 0.09 respectively, i. e. the smallest amount of feed was spent for suckling young animals’ fattening, and the largest – for weaning calves. Efficiency of fattening in rubles made 21.0, 56.4 and 48.0 rubles per 1 kg of weight gain, respectively, or in terms of 1 period of fattening of grown animals – 56.4 rubles, young animals raised at suckling – 6.8, and weaning calves – 19.2. The obtained data allows, through the biological methods and mathematical models individual for each group of cattle at fattening, to obtain optimal weight gain with minimized feed costs.

INTRODUCTION: Introduction of breast cancer screening programs has led to an increase in detection of non-palpable masses requiring core needle biopsy. Masses that fall into B3 category based on the histological results of core biopsy specimens, are of interest precisely because of their uncertain malignant potential and the lack of a final consensus on their management.OBJECTIVE: To analyze the 3-year experience of ultrasound guided vacuum-assisted biopsy (VAB) in patients with B3 breast lesions based on the results of histological examination of core biopsy specimens.MATERIALS AND METHODS: Between June 2019 and June 2022, 163 VABs were performed. The study group included patients with category 2–4 breast lesions according to the BI-RADS scoring system. The decision to perform VAB was made by the oncologist in each specific case on the basis of anamnesis, clinical data and the results of core biopsy histological examination: in cases of B3 or B2 lesions with a discordant radiologic pattern.RESULTS: In 155 (95.0%) cases, the removed lesions were benign, invasive cancer was detected in 2 (1.2%) cases, ductal cancer in situ in 6 cases (3.8%). The сomplications included: in 5 (3.1%) cases, patients had pain after the procedure, and in 37 (22.7%) cases there was a hematoma. In 5 (3.1%) patients, residual tissue was found at the site of removed lesions. CONCLUSIONS: VAB is a safe alternative to exploratory surgery in patients with B3 lesions diagnosed by core biopsy.

State of the greenland halibut stock in the Sea of Okhotsk fishing zone is evaluated as overfished with a high probability of 97.5 % by the index of fishable biomass, and the overfishing continued in 2021. This conclusion is based on results of double filtering the posterior parameter estimates in the state-space generalized surplus production model JABBA (Just Another Bayesian Biomass Assessment), with additional tuning of the new model taking into account these results. The overfishing was not caused by significant changes of environmental parameters (average SST and EOF modes of SST were examined), but its main reason was the overestimated target harvest rate established for the Far East of Russia as 10 % of the total biomass or a half of natural annual mortality (20 %). This incorrect value of the rate was based on incorrect determination of age structure and terminal age for the halibut by the fish scale method. The new approach of the stock status evaluation with JABBA model is independent on the age data. It includes the additional filtering of the JABBA model result, its refinement with stringent tuning using the algorithm of No-U-Turn sampler, and checking additional parameters on hyperstability or hypersensitivity. The new reference point of the target harvest rate is between 2.5 and 5.4 % (95 % credible interval) of the total biomass, with a median of about 3.75 %. This new value is slightly higher than that one used by ICES for greenland halibut in the northeastern Atlantic (3.5 % for fishable biomass, its reduction to 2.5 % is discussed), but these values cannot be compared because of different habitat conditions and probably different growth rate of greenland halibut in the Okhotsk Sea and the Atlantic. Researches on the optimal level of greenland halibut exploitation should be continued; anyway, the new reference point of the target harvest rate has to be implemented immediately and to be used at least until correction of the age-length key for greenland halibut in the Okhotsk Sea. The input data and Stan code of the new model are presented in the annexes.

The emergence of new technologies not only generates new methods of attacks, but also expands the existing list of threats, and, as you know, each threat can be carried out by a large number of different attacks. To date, there are methods based on various approaches to the study of information security threats, such as: assessment of the relevance of information security threats according to the FSTEC methodology of Russia, ATT&CK Matrix for Enterprise, Howard-Longstaff incident taxonomy, information security threat assessment study based on a security model. All of the above methods can be divided into two groups of threat assessment: quantitative and qualitative. In this connection, there is a need to conduct their research. The article presents a study of methods for assessing information security threats, conducted in various ways.

With the increasing importance of information, the problems with its effective protection increase. The methods of protection must be agreed with the relevant authorities and have the appropriate documentation.The purpose of the work is develop criteria for the selection of evaluation indicators of the organization for the control of licensing requirements and conditions in terms of monitoring information security systems.The following methods were used to perform the work: the method of analysis, generalization, comparison, classification. Solutions from Russian companies Positive Technologies and the Limited Liability Company "Innovation Technologies security", which are suitable for small businesses. The organization was evaluated by personnel and by means of protection. Criteria have been developed for the selection of organization evaluation indicators. Thanks to these indicators, it is easy to determine whether the organization meets the requirements or not, to determine the directions for improvement and protection of the enterprise.

In article, which aims to apply communication network security to improve the transmission quality of an educational video conference, an adaptive resource allocation algorithm is used to optimize the transmission quality over the network. The application of the protocol to improve the transmission quality of a video conference can increase the transmission speed, reduce the bit error rate. The algorithm minimizes latency using ant colony optimization and clustering, which ultimately achieves the goal of reducing power consumption and improving security. In order to improve system performance, the problem of power distribution must be taken into account when improving the transmission quality of an educational video conference. By analyzing the adaptive resource allocation mechanism applicable to network security while improving the transmission quality of an educational video conference, conditions were created for optimizing resource allocation.

Relevance. To increase the consumption of tomato and its diversity on the market, it is important to create new varieties that have economically valuable characteristics and are in demand among consumers. The aim of the research was to create a new determinant early-ripening variety of lettuce tomato, plastic, consistently productive, large-fruited, with dense pulp and high taste, tolerant to diseases.Materials and methods. The studies were carried out at the collection site of the Crimean OSS of the VIR branch (Krasnodar Region, Krymsk). The Agata Plus cultivar was obtained by crossing parental forms with subsequent multiple selections against a natural infectious background. As a mother, we used a salad variety of the Russian selection Agata – early maturing, undersized, with an average yield and fruits with good taste and average weight. The paternal form was a hybrid of the Dutch selection F1 Eploz, characterized by early maturity, medium height, medium leafiness, largefruited, dense pulp, consistently high yield and excellent taste of the fruit. The Agata tomato variety included in the State Register of the Russian Federation was taken as a standard.Results. Evaluation of breeding lines for a complex of biological, morphometric, economically valuable traits using individual and mass selections made it possible to obtain the best early ripening form of salad designation, marked by large fruit size, consistently high yield and good taste. In 2017, this form, called Agata Plus, was submitted to the State Variety Test, and since 2020 this variety has been approved for use.

Background. Breast cancer (BC) can be unifocal, multifocal, multicentric (MCBC) and bilateral according to number and localization of tumors. MCBC has different clinical and biological characteristics compared to unifocal BC, for example, a higher risk of lymph node involvement, a more aggressive natural history and a worse prognosis. Therefore, accurate diagnosis and detection of MCBC is one of the main tasks of imaging.Objective: to compare diagnostic accuracy of contrast enhanced spectral mammography (CESM) and digital mammography (DM) in the diagnosis of MCBC.Materials and methods. The study included 438 women with suspected BC. The results of DM and CESM were compared with the results of a pathomorphological study performed after surgery or biopsy to assess the effectiveness of imaging modalities. DM was performed for all women using a Senographe DS (GE, USA) unit. CESM was performed after intravenous injection of non-ionic contrast agents using injector with breast compression in the craniocaudal and mediolateral projections. The study was performed for both glands regardless of the location of the suspicious lesion in order to timely diagnose clinically asymptomatic bilateral cancer. The final diagnosis was made based on the results of the pathomorphological examination. MCBC was diagnosed when two or more tumors at a distance of 3 cm or more from each other were detected. Visualization of additional grouped calcifications of malignant type occupying a small portion of breast tissue (more than 15 pieces per 1 sq. cm) was considered as another mammographic sign of MCBC.Results. According to the pathomorphological examination BC was diagnosed in 154 (35 %) women out of 438 examined patients. MCBC was identified in 25 (16 %) of 154 patients. CESM was more effective than DM in detecting MCBC with sensitivity of 88.0 % vs 48.0 % (p = 0.002), accuracy of 92.2 % vs 90.9 %, negative predictive value of 97.6 % vs 90.8 % (p = 0.02), respectively. But the specificity was significantly higher in DM than in CESM – 99.2 % vs 93.0 % (p = 0.009), respectively, and the positive predictive value did not differ significantly (p = 0.12).Conclusion. CESM is a more sensitive method for diagnosing MCBC compared to DM with sensitivity of 88.0 % vs 48.0 %. The high negative predictive value of CESM (97.6 %) confirms the fact that this modality is an effective method for planning surgery and radiation therapy.

Bioprinting nerve conduits supplemented with glial or stem cells is a promising approach to promote axonal regeneration in the injured nervous system. In this study, we examined the effects of different compositions of bioprinted fibrin hydrogels supplemented with Schwann cells and mesenchymal stem cells (MSCs) on cell viability, production of neurotrophic factors, and neurite outgrowth from adult sensory neurons. To reduce cell damage during bioprinting, we analyzed and optimized the shear stress magnitude and exposure time. The results demonstrated that fibrin hydrogel made from 9 mg/mL of fibrinogen and 50IE/mL of thrombin maintained the gel’s highest stability and cell viability. Gene transcription levels for neurotrophic factors were significantly higher in cultures containing Schwann cells. However, the amount of the secreted neurotrophic factors was similar in all co-cultures with the different ratios of Schwann cells and MSCs. By testing various co-culture combinations, we found that the number of Schwann cells can feasibly be reduced by half and still stimulate guided neurite outgrowth in a 3D-printed fibrin matrix. This study demonstrates that bioprinting can be used to develop nerve conduits with optimized cell compositions to guide axonal regeneration.

Neuroblastoma is a complicated systemic malignant process that requires risk-adapted, multimodal therapy. Certainly, the dissemination of the tumor process is an extremely unfavorable prognosis for the patient’s life and health, however, local relapses can be cured successfully. The aim of the article is to demonstrate a rare clinical case of using SBRT in a patient with central nervous system neuroblastoma local relapse in the context of combined modality treatment.

Lung cancer has the highest morbidity rate among all malignant tumors in men and the highest mortality rate in men and women in Russia. In total, 49 145 new cases of lung cancer were registered (diagnosed) in Russia in 2019. The majority of cases are related to exogenic carcinogens and mainly tobacco smoke. For several decades surgical resection with preoperative cytotoxic therapy was an optimal approach for maximal cure rate. This year recommendations were updated with new strategies including adjuvant anti-PD-L1 atezolizumab following completion of chemotherapy in PD-L1 positive patients and osimertinib for EGFR mutated cases. For this moment available data suggest the increase in disease free survival. Strategic approach to treatment for inoperable patients varies according to the status of driver mutations. New approach includes pretreatment option of testing for a wide spectrum of alterations with NGS based panels. Significant changes were incorporated into treatment of ALK mutated NSCLC with two new options of brigatinib for TKI naive patients and lorlatinib for those who progress on second generation drugs. Treatment strategy for patients without activating mutations is based on PD-L1 status. Tsis year recommendations included atezolizumab as a new monotherapy option for patients with high depression of PD-L1. Also treatment options for pembrolizumab, nivolumab and atezolizimab were widened with prolonged treatment schedules.

The basic principles of the treatment of prostate cancer patients have underwent significant revisions in recent years. Modern radiotherapy techniques, which have demonstrated high efficacy and safety in long-term randomized trials, are beginning to take a leading position in the treatment of prostate cancer in an overwhelming number of clinical scenarios (National Comprehensive Cancer Network, 2021). Despite the obvious successes of radiation oncology, a number of important problems remain unresolved, first of all - the need to reduce the rates of radiation complications. The topographical anatomy of the prostate gland determines the main profiles of post-radiation damage: rectal and genitourinary radiation toxicity. The previous five years have been marked by a significant intensification of research work abroad aimed at clinical testing of a number of biopolymer compositions and products for use as spacers between irradiated structures and normal tissues. The experience has made it possible for the first time to consider the possibility of using spacers in radiotherapy treatment of prostate cancer in the modern recommendations of the European Association of Urology (2021). The analysis of the national literature shows a complete lack of publications on the possibilities of optimizing the radiation treatment of prostate cancer through the use of specers. The purpose of this work was the need to highlight this important and perspective clinical problem.

The pathophysiology of osteoarthritis (OA) includes the destruction of subchondral bone tissue and inflammation of the synovium. Thus, an effective disease-modifying treatment should act on both of these pathogenetic components. It is known that cSrc kinase is involved in bone and cartilage remodeling, and SYK kinase is associated with the inflammatory component. Thus the aim of this study was to characterize the mechanism of action and efficacy of a small molecule multikinase inhibitor MT-SYK-03 targeting SYK and cSrc kinases among others in different in vitro and in vivo arthritis models. The selectivity of MT-SYK-03 kinase inhibition was assayed on a panel of 341 kinases. The compound was evaluated in a set of in vitro models of OA and in vivo OA and RA models: surgically-induced arthritis (SIA), monosodium iodoacetate-induced arthritis (MIA), collagen-induced arthritis (CIA), adjuvant-induced arthritis (AIA). MT-SYK-03 inhibited cSrc and SYK with IC50 of 14.2 and 23 nM respectively. Only five kinases were inhibited > 90% at 500 nM of MT-SYK-03. In in vitro OA models MT-SYK-03 reduced hypertrophic changes of chondrocytes, bone resorption, and inhibited SYK-mediated inflammatory signaling. MT-SYK-03 showed preferential distribution to joint and bone tissue (in rats) and revealed disease-modifying activity in vivo by halving the depth of cartilage erosion in rat SIA model, and increasing the pain threshold in rat MIA model. Chondroprotective and antiresorptive effects were shown in a monotherapy regime and in combination with methotrexate (MTX) in murine and rat CIA models; an immune-mediated inflammation in rat AIA model was decreased. The obtained preclinical data support inhibition of cSrc and SYK as a viable strategy for disease-modifying treatment of OA. A Phase 2 clinical study of MT-SYK-03 is to be started.

Objective: to compare the immediate and long-term outcomes of patients with early breast cancer treated with intraoperative radial therapy depending on the biological subtype of breast cancer.Materials and methods. We prospectively evaluated number of recurrences, cosmetic effect and early treatment results of 104 patients with early breast cancer aged 66.72 ± 0.68 years old. The mean follow-up period was 36 months. The mean dose on the surface of applicator was 17.8 Gy, on the depth 0.5 cm – 8.8 Gy, on the depth 1.0 cm – 5 Gy. The mean time of radiation was 22.15 min ± 28.09 sec.Results. The local recurrence was in 3 patients. The first patient had triple negative breast cancer subtype, the second patient had luminal B HER2+, and the third one had luminal B HER2– subtype. Relapses occurred in 7, 14 and 20 months after the end of treatment respectively. The recurrence rate in patients with luminal B biological subtype was 10.71 %; in patients with triple negative subtype was 20 %. All recurrences were found in the area of the postoperative scar.Conclusion. The obtained results question the rationale for the use of the demonstrated method in patients with luminal B and triple negative molecular subtypes of tumors.

Charge transport in amorphous silicon nitride (Si3N4) is studied experimentally in a wide range of electric fields and temperatures. The experimental data are compared with the results of numerical calculations. To describe the ionization of deep centers (traps) in Si3N4, the Frenkel effect is used with the account for thermally assisted tunneling and the multiphonon mechanism. It is shown that the Frenkel effect with allowance for thermally assisted tunneling formally describes the experiment. However, an anomalously small frequency factor (109 s–1) and a large effective tunneling mass (3m0) must be used in calculations in this case. A satisfactory agreement between the results of experiments and calculations was attained using the theory of multiphonon ionization of traps with the following parameters: $$W_{T}^{{e,h}}$$ = 1.6 eV, $$W_{{{\text{opt}}}}^{{e,h}}$$ = 3.2 eV, $$W_{{{\text{ph}}}}^{{e,h}}$$ = 0.064 eV, $$m_{{e,h}}^{*}$$ = 0.6m0, and Ne,h = 6 × 1018 cm–3, which correspond to the thermal energy, optical energy, phonon energy, tunneling effective mass, and concentrations of electron and hole traps, respectively.

The Kasabach–Merritt phenomenon is a rare thrombocytopenic consumption of coagulopathy that occurs against the background of such vascular anomalies as kaposiform hemangioendothelioma and giant angioma. Timely diagnosis and treatment of Kasabach–Merritt syndrome which includes therapy for vascular tumors and life‑threatening conditions are critical in resolving this rare vascular anomaly. The aim of the study is to analyze radiation therapy as salvage therapy for kaposiform hemangioendothelioma and giant angioma associated with the Kasabach–Merritt phenomenon.

Background: Presently stem cell implantations are more and more developed and applied in clinics as alternative and complementary solutions to adult organ transplantation because of the growing demand and capacities to provide these interventions, while lack of donors is patent. But some limitations of stem cell use remain (for instance integration within the recipient organism of engineered tissues and organoids obtained in vitro from stem cells). The use of foetal organ implantation into adult animals may be an alternative for in vivo study of stem cell development and evaluation of the host participation to this process. The objectives of this work were to try different foetal organ implantations into adult animals (part I) and to evaluate their use for repairing experimental lesions of some adult organs (part II). Material and Methods: Part I: In > 650 experiments on rats and mice, different sites of foetal organ syngeneic implantation were proposed. Physiological (electric activity, motor and secretion activity) and morphological (per illumination, ultra sound and magnetic imaging, optic and electron microscopy) methods were used for implant development evaluation during up to 12 months. Part II: Foetal heart and foetal digestive organs were tested for both oesophagus circular defect and heart thermic lesion repair (38 and 29 rats).Trials with foetal pancreas implantation were provided in rats with diabetes induced by Streptozotocin and/or protein deficit and in a small pilot group of diabetic patients. Result: Part I: After a “dedifferentiation” phase, foetal organ implants could grow following ontogenetic pattern but – at least in our experimental conditions - some of them were not able to re-organize as a whole functional adult organ. The factors enhancing or limiting the organoid formation are considered. Part II: Foetal heart implants were proved to enhance heart lesion morphological and functional repair. Foetal oesophagus, stomach or intestine fragments combined with chitosan flaps ensured a complete restoration of the oesophagus wall after oval or segmental resection. Implantation of foetal pancreas was able to reverse Streptozotocin induced diabetes and to prevent chronic glucose disorders following protein deprivation in rats. In patients temporary positive influence was noted. Conclusion: Implantation of different foetal organs into syngeneic adults may constitute a valuable model for theoretical studies of in vivo stem cell differentiation and organoid growth. The ear implantation site deserves special attention. Some interesting applications are possible and worthwhile to be discussed and developed.

The article discusses modern approaches in the treatment of HER2-positive breast cancer (BC) with brain metastases (BM).The patients are subject to multidisciplinary, comprehensive and biologically – oriented treatment, with the involvement of a neurosurgeon and a radiation therapist to make a decision considering local treatment of BM, as well as a clinical oncologist to choose systemic drug therapy. Local treatment of HER2+ BC with BM patients includes surgical treatment and/or radiotherapy. Use of targeted anti-HER2 therapy changes “biology” of the disease from aggressive to indolent.In the prospective KAMILLA trial, clinically significant antitumor activity of trastuzumab emtansine was found for the first time both in patients with HER2+ BC with BM, who were previously treated with radiotherapy, and without radiotherapy in the anamnesis, which suggests the validity of further use of trastuzumab emtansine in this category of patients.The antitumor activity of trastuzumab emtansine in patients with HER2+ BC with BM was also confirmed in preclinical models. Despite the similar drug distribution in the tissues, trastuzumab emtansine, in contrast to trastuzumab, significantly slowed the growth of metastases, causing the induction of apoptosis in HER2+ BC models with BM in mice.

Relevance. Shallots (Allium ascalonicum L.) is a very useful and promising crop with high nutrition- al, taste and medicinal properties. Shallot is appreciated for its tender and juicy tasty greens, as well as early ripening of bulbs with a long shelf life. For widespread distribution and consumption, increasing demand and popularizing this culture, it is relevant to create new promising varieties with valuable economically useful characteristics that meet modern market requirements. When creating new varieties, it is important to search for sources of early maturity, keeping quality, productivity, yield and quality.The aim of the research was to study local varieties of shallots for a complex of valuable traits, select the best forms and create a new fruitful, white early-ripening white variety of the salad direction, possessing useful properties, good keeping quality of bulbs, resistant to unfavorable environmental factors for cultivation in the regions of the European part of Russia.Materials and methods. The initial material consisted of 18 varieties of the local population from the North Caucasian group, differing in morphometric, in particular the color of the bulb (yellow to white), biological and economic characteristics. The studies were carried out at the collection site of the Krymsk EBS, VIR Branch (Krasnodar Region, Krymsk). As a standard, we used two varieties included in the State Register with the color of the bulb from yellow (Off-season) to white (Snezhok).Results. The study of phenological, biological, morphometric and economic characteristics made it possible to select five promising forms that are valuable for the consumer: those with early maturity, productivity, light color, large long-term bulb, green feather productivity, winter hardiness and good taste. The best form – LSh 5-05, with a consistently high yield, long-term keeping quality of white bulbs, marketability, good mild taste and attractive appearance, is included in the State Register of the Russian Federation for admission to use as a shallot Blondin variety.

An effective physiotherapeutic method of abdominal decompression was offered with the aim of treating miscarriage and fetoplacental insufficiency in pregnants. 37 women with the pathology in question, who were in the II or III trimester of pregnancy, were examined. The results of the investigation show that abdominal decompression may become an alternative to orthodox methods of treatment and it may be widely applied in obstetrics.

The absolute atomic mass of