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2,305 results on '"A. Le Chevalier"'

2. Association of Molecular Profiles and Mutational Status With Distinct Histological Lung Adenocarcinoma Subtypes. An Analysis of the LACE-Bio Data

Author

Ashok Kumar, Prashanth, Karimi, Maryam, Basnet, Alina, Seymour, Lesley, Kratzke, Robert, Brambilla, Elizabeth, Le-Chevalier, Thierry, Soria, Jean-Charles, Olaussen, Ken André, Devarakonda, Siddhartha, Govindan, Ramaswamy, Tsao, Ming-Sound, Shepherd, Frances A, Michiels, Stefan, and Graziano, Stephen

Published
2023
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4. Full eradication of pre‐clinical human papilloma virus‐induced tumors by a lentiviral vaccine

Author

Laëtitia Douguet, Ingrid Fert, Jodie Lopez, Benjamin Vesin, Fabien Le Chevalier, Fanny Moncoq, Pierre Authié, Trang‐My Nguyen, Amandine Noirat, Fabien Névo, Catherine Blanc, Maryline Bourgine, David Hardy, François Anna, Laleh Majlessi, and Pierre Charneau

Subjects
early E6‐E7 oncoproteins, immuno‐oncotherapy, intra‐tumoral immune cells, lentiviral vector, tumor microenvironment, Medicine (General), R5-920, Genetics, QH426-470
Abstract

Abstract Human papillomavirus (HPV) infections are the cause of all cervical and numerous oropharyngeal and anogenital cancers. The currently available HPV vaccines, which induce neutralizing antibodies, have no therapeutic effect on established tumors. Here, we developed an immuno‐oncotherapy against HPV‐induced tumors based on a non‐integrative lentiviral vector encoding detoxified forms of the Early E6 and E7 oncoproteins of HPV16 and 18 genotypes, namely, “Lenti‐HPV‐07”. A single intramuscular injection of Lenti‐HPV‐07 into mice bearing established HPV‐induced tumors resulted in complete tumor eradication in 100% of the animals and was also effective against lung metastases. This effect correlated with CD8+ T‐cell induction and profound remodeling of the tumor microenvironment. In the intra‐tumoral infiltrates of vaccinated mice, the presence of large amounts of activated effector, resident memory, and transcription factor T cell factor‐1 (TCF‐1)+ “stem‐like” CD8+ T cells was associated with full tumor eradication. The Lenti‐HPV‐07‐induced immunity was long‐lasting and prevented tumor growth after a late re‐challenge, mimicking tumor relapse. Lenti‐HPV‐07 therapy synergizes with an anti‐checkpoint inhibitory treatment and therefore shows promise as an immuno‐oncotherapy against established HPV‐mediated malignancies.

Published
2023
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5. An intranasal lentiviral booster reinforces the waning mRNA vaccine-induced SARS-CoV-2 immunity that it targets to lung mucosa

Author

Vesin, Benjamin, Lopez, Jodie, Noirat, Amandine, Authié, Pierre, Fert, Ingrid, Le Chevalier, Fabien, Moncoq, Fanny, Nemirov, Kirill, Blanc, Catherine, Planchais, Cyril, Mouquet, Hugo, Guinet, Françoise, Hardy, David, Vives, Francina Langa, Gerke, Christiane, Anna, François, Bourgine, Maryline, Majlessi, Laleh, and Charneau, Pierre

Published
2022
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8. Development of an Environmental and Faecal DNA Tool for Monitoring the Spatial Distribution and Fish Predation on the Mountain Amphibian Calotriton asper.

Author

Deluen, Marine, Loot, Géraldine, Bertrand, Romain, Aubret, Fabien, Valentini, Alice, Calvez, Olivier, Delmas, Claudine, Le Chevalier, Hugo, Muratet, Jean, Pottier, Gilles, Ribéron, Alexandre, Rollet, Sylvain, Dubut, Vincent, Trochet, Audrey, and Blanchet, Simon

Subjects
BROWN trout, FRESHWATER fishes, WILDLIFE management, GEOGRAPHICAL distribution of fishes, SPECIES distribution
Abstract

Gathering ecological knowledge about species with cryptic behaviour can be challenging and strongly depends on human, financial and logistical resources. Yet, this is essential for the conservation of threatened and/or elusive species and wildlife management. The use of DNA tools is emerging as an efficient solution to overcome these constraints. Here, we developed specific primers and a droplet digital PCR (ddPCR) protocol for the quantitative detection of the endemic amphibian Calotriton asper in freshwater and fish faeces. After successful in silico validation of the developed primers, we tested their validity in the wild. First, using an environmental DNA approach, we confirmed the presence of C. asper in eight out of 13 sites deemed climatically suitable for the species, although it had never been observed there before. Then, we sampled brown trout (Salmo trutta) living in sympatry with C. asper and, through fish faeces analyses, confirmed that brown trout are predators of C. asper. These molecular tools proved efficient for monitoring (i) elusive species such as C. asper in the wild, allowing for better characterization of their current distribution and habitat use, and (ii) predation events by fish, which might be a significant pressure on low‐density and isolated C. asper populations. We provide a valuable, low‐cost and complementary tool for environmental managers and scientists that should help improve the development of fine‐tuned conservation and management plans for C. asper. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Genetic and demographic trends from rear to leading edge are explained by climate and forest cover in a cold-adapted ectotherm

Author

Dupoué, Andréaz, Trochet, Audrey, Richard, Murielle, Sorlin, Mahaut, Guillon, Michaël, Teulieres-Quillet, Jules, Vallé, Clément, Rault, Cyrielle, Berroneau, Maud, Berroneau, Matthieu, Lourdais, Olivier, Blaimont, Pauline, Bertrand, Romain, Pottier, Gilles, Calvez, Olivier, Guillaume, Olivier, Le Chevalier, Hugo, Souchet, Jérémie, Le Galliard, Jean-François, Clobert, Jean, and Aubret, Fabien

Published
2021

14. In vivo characterization of the activities of novel cyclodipeptide oxidases: new tools for increasing chemical diversity of bioproduced 2,5-diketopiperazines in Escherichia coli

Author

Fabien Le Chevalier, Isabelle Correia, Lucrèce Matheron, Morgan Babin, Mireille Moutiez, Nicolas Canu, Muriel Gondry, Olivier Lequin, and Pascal Belin

Subjects
Synthetic biology, Combinatorial biosynthesis, Natural products, 2,5-diketopiperazine, Cyclodipeptide synthase, Cyclodipeptide oxidase, Microbiology, QR1-502
Abstract

Abstract Background Cyclodipeptide oxidases (CDOs) are enzymes involved in the biosynthesis of 2,5-diketopiperazines, a class of naturally occurring compounds with a large range of pharmaceutical activities. CDOs belong to cyclodipeptide synthase (CDPS)-dependent pathways, in which they play an early role in the chemical diversification of cyclodipeptides by introducing Cα-Cβ dehydrogenations. Although the activities of more than 100 CDPSs have been determined, the activities of only a few CDOs have been characterized. Furthermore, the assessment of the CDO activities on chemically-synthesized cyclodipeptides has shown these enzymes to be relatively promiscuous, making them interesting tools for cyclodipeptide chemical diversification. The purpose of this study is to provide the first completely microbial toolkit for the efficient bioproduction of a variety of dehydrogenated 2,5-diketopiperazines. Results We mined genomes for CDOs encoded in biosynthetic gene clusters of CDPS-dependent pathways and selected several for characterization. We co-expressed each with their associated CDPS in the pathway using Escherichia coli as a chassis and showed that the cyclodipeptides and the dehydrogenated derivatives were produced in the culture supernatants. We determined the biological activities of the six novel CDOs by solving the chemical structures of the biologically produced dehydrogenated cyclodipeptides. Then, we assessed the six novel CDOs plus two previously characterized CDOs in combinatorial engineering experiments in E. coli. We co-expressed each of the eight CDOs with each of 18 CDPSs selected for the diversity of cyclodipeptides they synthesize. We detected more than 50 dehydrogenated cyclodipeptides and determined the best CDPS/CDO combinations to optimize the production of 23. Conclusions Our study establishes the usefulness of CDPS and CDO for the bioproduction of dehydrogenated cyclodipeptides. It constitutes the first step toward the bioproduction of more complex and diverse 2,5-diketopiperazines.

Published
2020
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15. A simplified interventional mapping system (SIMS) for the selection of combinations of targeted treatments in non-small cell lung cancer

Author

Lazar, Vladimir, Rubin, Eitan, Depil, Stephane, Pawitan, Yudi, Martini, Jean-François, Gomez-Navarro, Jesus, Yver, Antoine, Kan, Zhengyin, Dry, Jonathan R, Kehren, Jeanne, Validire, Pierre, Rodon, Jordi, Vielh, Philippe, Ducreux, Michel, Galbraith, Susan, Lehnert, Manfred, Onn, Amir, Berger, Raanan, Pierotti, Marco A, Porgador, Angel, Pramesh, CS, Ye, Ding-wei, Carvalho, Andre L, Batist, Gerald, Le Chevalier, Thierry, Morice, Philippe, Besse, Benjamin, Vassal, Gilles, Mortlock, Andrew, Hansson, Johan, Berindan-Neagoe, Ioana, Dann, Robert, Haspel, Joel, Irimie, Alexandru, Laderman, Steve, Nechushtan, Hovav, Omari, Amal S Al, Haywood, Trent, Bresson, Catherine, Soo, Khee Chee, Osman, Iman, Mata, Hilario, Lee, Jack J, Jhaveri, Komal, Meurice, Guillaume, Palmer, Gary, Lacroix, Ludovic, Koscielny, Serge, Eterovic, Karina Agda, Blay, Jean-Yves, Buller, Richard, Eggermont, Alexander, Schilsky, Richard L, Mendelsohn, John, Soria, Jean-Charles, Rothenberg, Mace, Scoazec, Jean-Yves, Hong, Waun Ki, and Kurzrock, Razelle

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Lung, Biotechnology, Cancer, Lung Cancer, Adult, Aged, Aged, 80 and over, Antineoplastic Combined Chemotherapy Protocols, Carcinoma, Non-Small-Cell Lung, Female, Humans, Lung Neoplasms, Male, Middle Aged, Molecular Targeted Therapy, Precision Medicine, Transcriptome, Tri-therapy, NSCLC, targeted therapies, algorithm, pathway, tri-therapy, Oncology and carcinogenesis
Abstract

Non-small cell lung cancer (NSCLC) is a leading cause of death worldwide. Targeted monotherapies produce high regression rates, albeit for limited patient subgroups, who inevitably succumb. We present a novel strategy for identifying customized combinations of triplets of targeted agents, utilizing a simplified interventional mapping system (SIMS) that merges knowledge about existent drugs and their impact on the hallmarks of cancer. Based on interrogation of matched lung tumor and normal tissue using targeted genomic sequencing, copy number variation, transcriptomics, and miRNA expression, the activation status of 24 interventional nodes was elucidated. An algorithm was developed to create a scoring system that enables ranking of the activated interventional nodes for each patient. Based on the trends of co-activation at interventional points, combinations of drug triplets were defined in order to overcome resistance. This methodology will inform a prospective trial to be conducted by the WIN consortium, aiming to significantly impact survival in metastatic NSCLC and other malignancies.

Published
2015

16. Full-Lung Prophylaxis against SARS-CoV-2 by One-Shot or Booster Intranasal Lentiviral Vaccination in Syrian Golden Hamsters

Author

Benjamin Vesin, Pierre Authié, Catherine Blanc, Ingrid Fert, Amandine Noirat, Fabien Le Chevalier, Yu Wei, Min-Wen Ku, Kirill Nemirov, François Anna, David Hardy, Cyril Planchais, Hugo Mouquet, Françoise Guinet, Pierre Charneau, Laleh Majlessi, and Maryline Bourgine

Subjects
lentiviral vectors, SARS-CoV-2 variants, intranasal vaccination, mucosal immunity, lung inflammation, cross-neutralization, Medicine
Abstract

Following the breakthrough of numerous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in recent months and the incomplete efficiency of the currently available vaccines, development of more effective vaccines is desirable. Non-integrative, non-cytopathic and non-inflammatory lentiviral vectors elicit sterilizing prophylaxis against SARS-CoV-2 in preclinical animal models and are particularly suitable for mucosal vaccination, which is acknowledged as the most effective in reducing viral transmission. Here, we demonstrate that a single intranasal administration of a vaccinal lentiviral vector encoding a stabilized form of the original SARS-CoV-2 Spike glycoprotein induces full-lung protection of respiratory tracts and strongly reduces pulmonary inflammation in the susceptible Syrian golden hamster model against the prototype SARS-CoV-2. In addition, we show that a lentiviral vector encoding stabilized Spike of SARS-CoV-2 Beta variant (LV::SBeta-2P) prevents pathology and reduces infectious viral loads in lungs and nasal turbinates following inoculation with the SARS-CoV-2 Omicron variant. Importantly, an intranasal boost with LV::SBeta-2P improves cross-seroneutralization much better in LV::SBeta-2P-primed hamsters than in their counterparts primed with an LV-encoding Spike from the ancestral SARS-CoV-2. These results strongly suggest that an immune imprint with the original Spike sequence has a negative impact on cross-protection against new variants. Our results tackle the issue of vaccine effectiveness in people who have already been vaccinated and have vanished immunity and indicate the efficiency of LV-based intranasal vaccination, either as a single dose or as booster.

Published
2022
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18. LACE-Bio: Validation of Predictive and/or Prognostic Immunohistochemistry/Histochemistry-based Biomarkers in Resected Non–small-cell Lung Cancer

Author

Seymour, Lesley, Le Teuff, Gwénaël, Brambilla, Elisabeth, Shepherd, Frances A., Soria, Jean-Charles, Kratzke, Robert, Graziano, Stephen, Douillard, Jean-Yves, Rosell, Rafael, Reiman, Anthony, Lacas, Benjamin, Lueza, Beranger, Aviel-Ronen, Sarit, McLeer, Anne, Le Chevalier, Thierry, Pirker, Robert, Filipits, Martin, Dunant, Ariane, Pignon, Jean-Pierre, and Tsao, Ming-Sound

Published
2019
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19. The Accuracy of Clinical Staging of Stage I-IIIa Non-Small Cell Lung Cancer: An Analysis Based on Individual Participant Data

Author

Burdett, Sarah, Rydzewska, Larysa H.M., Tierney, Jayne F., Auperin, Anne, Le Chevalier, Thierry, Le Pechoux, Cécile, Pignon, Jean-Pierre, Arriagada, Rodrigo, Johnson, David H., van Meerbeeck, Jan, Parmar, Mahesh K.B., Stephens, Richard J., Stewart, Lesley A., Bunn, Paul A., Dautzenberg, Bertrand, Gilligan, David, Groen, Harry, Knuuttila, Aija, Vallieres, Eric, Rosell, Rafael, Roth, Jack, Scagliotti, Giorgio, Tsuboi, Masahiro, Waller, David, Westeel, Virginie, Wu, Yi-Long, Yang, Xue-Ning, Navani, Neal, and Fisher, David J.

Published
2019
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20. Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura

Author

Hugo Le Chevalier, Neus Marí‐Mena, Belén Carro, Jérôme G. Prunier, Coralie Bossu, Elodie Darnet, Jérémie Souchet, Olivier Guillaume, Olivier Calvez, Romain Bertrand, Laurent Barthe, Gilles Pottier, Albert Martínez‐Sylvestre, Isabel Verdaguer‐Foz, Marc Mossoll‐Torres, Audrey Trochet, and Fabien Aubret

Subjects
Natrix maura, polymorphic microsatellites, population genetics, viperine snake, Ecology, QH540-549.5
Abstract

Abstract Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 high‐quality polymorphic markers. These markers will be extremely useful in identifying fine‐scale genetic structures and providing insight into conservation management plans of this species.

Published
2019
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21. Evaluation of patients’ needs to design and assess a patient education program in cancer pain

Author

Prevost V, Delorme C, Heutte N, Leconte A, Bechet C, Licaj I, Bignon R, Bisson C, Cauchin S, Gicquère M, Grach MC, Guillaumé C, Le Garrec J, Ropartz MC, Roux N, Sep Hieng V, Le Chevalier A, and Clarisse B

Subjects
cancer pain, pain management, pain assessment, patient education, educative needs, questionnaire, Medicine (General), R5-920
Abstract

V Prevost,1 C Delorme,2,3 N Heutte,4 A Leconte,4 C Bechet,5 I Licaj,4 R Bignon,3,6 C Bisson,2,3 S Cauchin,3,7 M Gicquère,3,8 MC Grach,3,8 C Guillaumé,3,9 J Le Garrec,3,7 MC Ropartz,3,10 N Roux,3,9 V Sep Hieng,3,6 A Le Chevalier,3,10 B Clarisse41UMR 1086 Inserm ANTICIPE (Interdisciplinary Research Unit for Cancer Treatment and Prevention) and University of Normandy, Caen, France; 2Pain Assessment and Treatment Centre, Bayeux Hospital, Bayeux, France; 3Regional Pain Network for Lower Normandy, Bayeux, France; 4Clinical Research Unit, Centre François Baclesse, Caen, France; 5Pharmacie de la Croix d’Or, Paris, France; 6Pain and Palliative Care Unit, Lisieux Hospital, Lisieux, France; 7Pain Assessment and Treatment Consultation, Alençon-Mamers Intercommunal Hospital, Alençon, France; 8Pain and Palliative Care Unit, Centre François Baclesse, Caen, France; 9Pain and Palliative Care Unit, University Hospital, Caen, France; 10Pain Assessment and Treatment Centre, Avranches-Granville Hospital, Avranches, FrancePurpose: Patient education constitutes a relevant strategy to improve pain management. In the field of therapeutic patient education (TPE), we aimed 1) to assess pain impact in cancer patients, 2) to identify patients’ educative needs in pain management, and 3) to refine research criteria for its future evaluation.Patients and methods: Pain intensity, relief and interference were assessed in 75 cancer patients with unbalanced background pain. Self-assessment questionnaire evaluated i) patients’ pain management and ii) their knowledge and needs in TPE.Results: Most patients experienced pain for more than 6 months and 41.6% reported adequate pain relief. Understanding pain and pain management were major patients’ preferences (>58%). Most patients declared they knew their pain treatments, but fewer than half of them were able to name them. However, education concerning pain treatment was considered as essential in

Published
2019

22. Construction et évaluation d’un programme d’éducation thérapeutique du patient dans le domaine de la douleur liée au cancer

Author

Prevost, Virginie, Clarisse, Bénédicte, Heutte, Natacha, Leconte, Alexandra, Bisson, Cécile, Bignon, Rachel, Cauchin, Sonia, Feuillet, Maryline, Gehanne, Sylvie, Gicquère, Maud, Grach, Marie-Christine, Guillaumé, Cyril, Le Gal, Christine, Le Garrec, Joelle, Lecaer, Franck, Lepleux, Isabelle, Millet, Anne-Laure, Ropartz, Marie-Claude, Roux, Nathalie, Sep Hieng, Virith, Van Delook, Carole, Bechet, Constance, Le Chevalier, Aline, and Delorme, Claire

Published
2018
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28. Embryonic and juvenile snakes (Natrix maura, Linnaeus 1758) compensate for high elevation hypoxia via shifts in cardiovascular physiology and metabolism

Author

Souchet, Jérémie, primary, Josserand, Alicia, additional, Darnet, Elodie, additional, Le Chevalier, Hugo, additional, Trochet, Audrey, additional, Bertrand, Romain, additional, Calvez, Olivier, additional, Martinez‐Silvestre, Albert, additional, Guillaume, Olivier, additional, Mossoll‐Torres, Marc, additional, Pottier, Gilles, additional, Philippe, Hervé, additional, Aubret, Fabien, additional, and Gangloff, Eric J., additional

Published
2023
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29. Full eradication of pre‐clinical human papilloma virus‐induced tumors by a lentiviral vaccine

Author

Douguet, Laëtitia, primary, Fert, Ingrid, additional, Lopez, Jodie, additional, Vesin, Benjamin, additional, Le Chevalier, Fabien, additional, Moncoq, Fanny, additional, Authié, Pierre, additional, Nguyen, Trang‐My, additional, Noirat, Amandine, additional, Névo, Fabien, additional, Blanc, Catherine, additional, Bourgine, Maryline, additional, Hardy, David, additional, Anna, François, additional, Majlessi, Laleh, additional, and Charneau, Pierre, additional

Published
2023
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33. Therapeutic Patient Education in Cancer Pain Management: from Practice to Research: Proposals and Strategy of the French EFFADOL Program

Author

Prevost, Virginie, Clarisse, Bénédicte, Heutte, Natacha, Leconte, Alexandra, Bisson, Cécile, Bignon, Rachel, Cauchin, Sonia, Feuillet, Maryline, Gehanne, Sylvie, Gicquère, Maud, Grach, Marie-Christine, Guillaumé, Cyril, Le Gal, Christine, Le Garrec, Joelle, Lecaer, Franck, Lepleux, Isabelle, Millet, Anne-Laure, Ropartz, Marie-Claude, Roux, Nathalie, Hieng, Virith Sep, Van Delook, Carole, Le Chevalier, Aline, and Delorme, Claire

Published
2018
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34. 3D structures of the Plasmodium vivax subtilisin-like drug target SUB1 reveal conformational changes to accommodate a substrate-derived α-ketoamide inhibitor

Author

Martinez, Mariano, primary, Batista, Fernando A., additional, Maurel, Manon, additional, Bouillon, Anthony, additional, Ortega Varga, Laura, additional, Wehenkel, Anne Marie, additional, Le Chevalier-Sontag, Lucile, additional, Blondel, Arnaud, additional, Haouz, Ahmed, additional, Hernandez, Jean-François, additional, Alzari, Pedro M., additional, and Barale, Jean-Christophe, additional

Published
2023
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35. Dosiomics-Based Prediction of Radiation-Induced Valvulopathy after Childhood Cancer

Author

Chounta, Stefania, primary, Allodji, Rodrigue, additional, Vakalopoulou, Maria, additional, Bentriou, Mahmoud, additional, Do, Duyen Thi, additional, De Vathaire, Florent, additional, Diallo, Ibrahima, additional, Fresneau, Brice, additional, Charrier, Thibaud, additional, Zossou, Vincent, additional, Christodoulidis, Stergios, additional, Lemler, Sarah, additional, and Letort Le Chevalier, Veronique, additional

Published
2023
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37. Prognostic and Predictive Effect of TP53 Mutations in Patients with Non–Small Cell Lung Cancer from Adjuvant Cisplatin–Based Therapy Randomized Trials: A LACE-Bio Pooled Analysis

Author

Ma, Xiaoli, Le Teuff, Gwénaël, Lacas, Benjamin, Tsao, Ming Sound, Graziano, Stephen, Pignon, Jean-Pierre, Douillard, Jean-Yves, Le Chevalier, Thierry, Seymour, Lesley, Filipits, Martin, Pirker, Robert, Jänne, Pasi A., Shepherd, Frances A., Brambilla, Elisabeth, Soria, Jean-Charles, and Hainaut, Pierre

Published
2016
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41. The European Cancer Patient’s Bill of Rights, update and implementation 2016

Author

Lawler, Mark, Banks, Ian, Law, Kate, Albreht, Tit, Armand, Jean-Pierre, Barbacid, Mariano, Barzach, Michèle, Bergh, Jonas, Cameron, David, Conte, Pierfranco, de Braud, Filippo, de Gramont, Aimery, De Lorenzo, Francesco, Diehl, Volker, Diler, Sarper, Erdem, Sema, Geissler, Jan, Gore-Booth, Jola, Henning, Geoffrey, Højgaard, Liselotte, Horgan, Denis, Jassem, Jacek, Johnson, Peter, Kaasa, Stein, Kapitein, Peter, Karjalainen, Sakari, Kelly, Joan, Kienesberger, Anita, La Vecchia, Carlo, Lacombe, Denis, Lindahl, Tomas, Löwenberg, Bob, Luzzatto, Lucio, Malby, Rebecca, Mastris, Ken, Meunier, Françoise, Murphy, Martin, Naredi, Peter, Nurse, Paul, Oliver, Kathy, Pearce, Jonathan, Pelouchov, Jana, Piccart, Martine, Pinedo, Bob, Spurrier-Bernard, Gilly, Sullivan, Richard, Tabernero, Josep, Van de Velde, Cornelis, van Herk, Bert, Vedsted, Peter, Waldmann, Anita, Weller, David, Wilking, Nils, Wilson, Roger, Yared, Wendy, Zielinski, Christoph, zur Hausen, Harald, Le Chevalier, Thierry, Johnston, Patrick, and Selby, Peter

Published
2016
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42. Novel active agents in patients with advanced NSCLC without driver mutations who have progressed after first-line chemotherapy

Author

Manegold, Christian, Adjei, Alex, Bussolino, Federico, Cappuzzo, Federico, Crino, Lucio, Dziadziuszko, Rafal, Ettinger, David, Fennell, Dean, Kerr, Keith, Le Chevalier, Thierry, Leighl, Natasha, Papotti, Mauro, Paz-Ares, Luis, Pérol, Maurice, Peters, Solange, Pirker, Robert, Quoix, Elisabeth, Reck, Martin, Smit, Egbert, Vokes, Everett, van Zandwijk, Nico, and Zhou, Caicun

Published
2016
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46. Mutations in ppe38 block PE_PGRS secretion and increase virulence of Mycobacterium tuberculosis

Author

Ates, Louis S., Dippenaar, Anzaan, Ummels, Roy, Piersma, Sander R., van der Woude, Aniek D., van der Kuij, Kim, Le Chevalier, Fabien, Mata-Espinosa, Dulce, Barrios-Payán, Jorge, Marquina-Castillo, Brenda, Guapillo, Carolina, Jiménez, Connie R., Pain, Arnab, Houben, Edith N. G., Warren, Robin M., Brosch, Roland, Hernández-Pando, Rogelio, and Bitter, Wilbert

Published
2018
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47. Mice Humanized for MHC and hACE2 with High Permissiveness to SARS-CoV-2 Omicron Replication

Author

Fabien Le Chevalier, Pierre Authié, Sébastien Chardenoux, Maryline Bourgine, Benjamin Vesin, Delphine Cussigh, Yohann Sassier, Ingrid Fert, Amandine Noirat, Kirill Nemirov, François Anna, Marion Bérard, Françoise Guinet, David Hardy, Pierre Charneau, François Lemonnier, Francina Langa-Vives, Laleh Majlessi, Laboratoire commun Pasteur-TheraVectys, Institut Pasteur [Paris] (IP)-TheraVectys-Université Paris Cité (UPCité), Centre d'Ingénierie génétique murine - Mouse Genetics Engineering Center (CIGM), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Animalerie Centrale (plateforme) - Central Animal Facility (platform) (C2RA), Lymphocytes et Immunité - Lymphocytes and Immunity, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Plate-Forme d’Histopathologie / Histopathology Platform, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and Institut Pasteur et Theravectys

Subjects
HLA, Infectious Diseases, SARS-CoV-2, [SDV]Life Sciences [q-bio], Immunology, Transgenesis, Omicron variant, Microbiology, COVID-19 animal model
Abstract

International audience; Human Angiotensin-Converting Enzyme 2 (hACE2) is the major receptor enabling host cell invasion by SARS-CoV-2 via interaction with Spike. The murine ACE2 does not interact efficiently with SARS CoV-2 Spike and therefore the laboratory mouse strains are not permissive to SARS-CoV-2 replication. Here, we generated new hACE2 transgenic mice, which harbor the hACE2 gene under the human keratin 18 promoter, in “HHD-DR1” background. HHD-DR1 mice are fully devoid of murine Major Histocompatibility Complex (MHC) molecules of class-I and -II and express only MHC molecules from Human Leukocyte Antigen (HLA) HLA 02.01, DRA01.01, DRB1.01.01 alleles, widely expressed in human populations. We selected three transgenic strains, with various hACE2 mRNA expression levels and distinctive profiles of lung and/or brain permissiveness to SARS-CoV-2 replication. These new hACE2 transgenic strains display high permissiveness to the replication of SARS-CoV-2 Omicron sub-variants, while the previously available B6.K18-ACE22Prlmn/JAX mice have been reported to be poorly susceptible to infection with Omicron. As a first application, one of these MHC- and ACE2-humanized strains was successfully used to show the efficacy of a lentiviral-based COVID-19 vaccine

Published
2023
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