Search

To explore the sources of and associated risks with drinking water contamination in low-income, densely populated urban areas, we collected human feces, domesticated animal feces, and source and stored drinking water samples in Nairobi, Kenya in 2019; and analyzed them using microbial source tracking (MST) and enteric pathogen TaqMan Array Cards (TACs). We established host-pathogen relationships in this setting, including detecting Shigella and Norovirus─which are typically associated with humans─in dog feces. We evaluated stored and source drinking water quality using indicator Escherichia coli (E. coli), MST markers, and TACs, detecting pathogen targets in drinking water that were also detected in specific animal feces. This work highlights the need for further evaluation of host-pathogen relationships and the directionality of pathogen transmission to prevent the disease burden associated with unsafe drinking water and domestic animal ownership.

BACKGROUND: One-fifth of the global population is infected with soil-transmitted helminths (STH). Mass drug administration (MDA) with deworming medication is widely implemented to control morbidity associated with STH infections. However, surveillance of human infection prevalence by collecting individual stool samples is time-consuming, costly, often stigmatized, and logistically challenging. Current methods of STH detection are poorly sensitive, particularly in low-intensity and low-prevalence populations. METHODOLOGY/PRINCIPAL FINDINGS: We aimed to develop a sensitive and specific molecular method for detecting STH DNA in large volumes of soil (20 g) by conducting laboratory and proof of concept studies across field sites in Kenya, Benin, and India. We collected human stool (n = 669) and soil (n = 478) from 322 households across the three study sites. We developed protocols for DNA extraction from 20 g of soil and qPCR to detect Ascaris lumbricoides, Trichuris trichiura, Necator americanus, and Ancylostoma duodenale. Agreement between detection of STH via qPCR, digital droplet PCR (ddPCR), and microscopy-based methods was assessed using the Cohens Kappa statistic. Finally, we estimated associations between soil characteristics and detection of STH in soil by qPCR, as well as between STH detected in soil and STH detected in stool from matched households, adjusting for soil characteristics. The overall prevalence of STH in soil by qPCR was 31% for A. lumbricoides, 3% for T. trichiura, and 13% for any hookworm species. ddPCR and qPCR performed similarly. However, there was poor agreement between STH detected in soil by qPCR versus light microscopy. Microscopy underestimated the prevalence of A. lumbricoides and N. americanus and overestimated T. trichiura. Detection of an STH species in household soil was strongly associated with increased odds of a household member being infected with that same species. CONCLUSIONS/SIGNIFICANCE: Soil surveillance for STH has several benefits over stool-based surveillance, including lower cost and higher success rates for sample collection. Considering that delivery of MDA occurs at the community level, environmental surveillance using molecular methods could be a cost-effective alternate strategy for monitoring STH in these populations.

Se realizó una investigación con el objetivo de identificar las tendencias de la producción científica cubana sobre algunas Tecnologías Habilitadoras para la Transformación Digital (THD) en Cuba indexadas en Scopus en el periodo 1997-2022 y analizar el comportamiento a partir de indicadores bibliométricos como: estructura intelectual, conceptual y social de las mismas. Fue realizado a partir de la combinación de dichos indicadores y de técnicas bibliométricas para la obtención de rasgos de su comportamiento y de las prácticas de producción de la comunidad científica especializada. Entre los resultados se halló que la evolución anual mostró un crecimiento lineal tanto en documentos como en el número de citas recibidas, producción hallada fundamentalmente en revistas extranjeras y en la zona núcleo del tema abordado. Hubo coincidencia en autor destacado en la red de co-citación y líder de una de las redes colaborativas. Las fuentes más referenciadas fueron: Lecture Notes in Computer Science, Information Scienes y Neurocomputing. Predominó la participación de las universidades y en colaboraciones con España y Bélgica. Hubo mayoría de las temáticas artificial inteligente, machine learning, learning systems, human computer interaction y data minning relacionados con la salud. El estudio resaltó la importancia de la contextualización de los indicadores bibliométricos. Además, permitió comparar las tendencias mostradas por la producción científica cubana con respecto al contexto internacional. Se ofrecen ideas finales sobre futuras líneas de investigación y la importancia de los resultados encontrados.

Background/Purpose: Facial asymmetry is common in Class III patients requiring orthognathic surgery. This study aimed to analyze jaw bone position after surgical-orthodontic treatment in three types of skeletal Class III asymmetry patients. Methods: The retrospective study included 30 Class III patients who underwent surgical-orthodontic treatment comprising LeFort I osteotomy and bilateral sagittal split osteotomy (BSSO) without genioplasty. Cone-beam computed tomography (CBCT) images obtained before surgery (T1) and after post-surgical orthodontic treatment (T2) were superimposed with voxel-based registration. Patients were classified into three groups based on T1 CBCT scans. Groups 1 and 2 exhibited menton and ramus deviated to the same side. Menton deviation was larger than ramus width asymmetry in group 1, while the reverse was true for group 2. Group 3 had menton deviation contralateral to the side with greater ramus width. Results: Menton deviation after treatment was improved in all groups. Ramus width asymmetry and coronal ramus angle difference decreased in groups 1 and 2. Neither improvement nor deterioration of ramus width asymmetry was noted for group 3. Comparing to groups 1 and 2, group 3 had greater roll and yaw rotations of distal segment, more upward pitch of proximal segment on chin deviation side, and largest inward yaw as well as backward translation of proximal segment on non-deviation side. Conclusion: The positional changes of osteotomy segments differed among three types of mandibular asymmetry. Special attention should be given to the atypical mandibular asymmetry with mandibular body and ramus deviating to opposite directions during surgical correction of jaw deflection.

Background/purpose: The use of computed tomography (CT) for craniofacial measurements is common in medical imaging, but concerns about accuracy and reliability persist, especially with different CT technologies. This study assessed the accuracy of twenty-six common measurements on consecutive CT images from the same patients, using multidetector CT (MDCT) and cone-beam CT (CBCT) with two software programs (Amira and Dolphin). Materials and methods: Ten adult subjects with consecutive CBCT scans within one year were randomly selected. Another ten subjects with consecutive MDCT scans were paired with the CBCT group based on age, gender, race, occlusion, and craniofacial pattern. All digital imaging and communications in medicine (DICOM) files were randomly coded and analyzed using the two software programs. Intra-examiner reliability was assessed using the intraclass correlation coefficient. Successive measurement errors from consecutive scans for both imaging modalities and software programs were compared. Results: For most skeletal linear and angular measurements, Dolphin showed greater successive measurement errors compared to Amira. Eight of the 26 common measurements had errors greater than one unit (millimeter or degree). Despite almost perfect intra-examiner reliability for upper airway analysis, average successive measurement errors were notably high, particularly for intraoral and oropharyngeal airway volumes. The successive Dolphin measurement error for oropharyngeal airway volume on CBCT images was over three times that on MDCT images. Conclusion: Given the substantial successive measurement errors observed during consecutive CT scanning for the upper airway, this study does not support the quantitative use of CT for analyzing changes in airway dimensions for research purposes.

Streptococcus pneumoniae (S. pneumoniae), which is a Gram-positive diplococcus, has emerged as a significant human pathogen. It is a primary cause of bacterial pneumonia, otitis media, meningitis, and septicemia, leading to a considerable impact on global morbidity and mortality. The investigation of S. pneumoniae and its virulence factors has resulted in the identification of surface endonuclease A (EndA). EndA functions in DNA uptake during natural transformation and plays a significant role in gene transfer. The ability of S. pneumoniae to degrade neutrophil extracellular traps (NETs) enhances its virulence and invasive potential in pneumococcal infections. NETosis occurs when neutrophils release chromatin into the extracellular space to form NETs, capturing and neutralizing pathogens. Currently, NETosis can be induced by several microbes, particulate matter, and sterile stimuli through distinct cellular mechanisms, and this includes the involvement of EndA in S. pneumoniae. Here, we reviewed the cellular functions of EndA, its role in S. pneumoniae as a virulence factor in relation to NETosis, its relationship to immunogenicity, and its involvement in several diseases. The discovery of this relationship would significantly impact therapeutic technology in reducing disease burden, especially pneumococcal infections.

Cerebral air embolism after removal of central venous catheter (CVC) is a rare complication but can lead to fatal outcomes. We report a rare case of both cerebral venous and arterial embolism occurring in a patient with underlying scleroderma-related interstitial lung disease (SSc-ILD) and pulmonary hypertension following removal of percutaneous introducer sheath for pulmonary artery catheterization. We discuss the mechanisms, pathophysiology, management and prevention of cerebral air embolism.

Purpose: The purpose of this paper is to evaluate the effectiveness of a career management program embedded within a business module, with a specific focus on enhancing the employability of students. Design/methodology/approach: The study involved five one-hour workshops based on the DOTS model, covering aspects like resume writing and professional communication. Surveys, using a structured questionnaire and a Likert scale, measured participants' perceived employability. The pre-survey included demographic details, while the post-survey evaluated the effectiveness of the Career development learning workshop. Incentives and QR codes were employed to boost participation, and data were collected at the semester's start and end, with responses linked through students' phone numbers. Findings: The results confirmed a positive connection between the perceived effectiveness of skill programs and students' employability. Integration of career management into the curriculum cultivates a heightened professional mindset and boosts employability. Validation through a paired-samples t-test demonstrates a substantial increase in perceived employability after program participation, affirming the program's role in fostering psychosocial empowerment and elevating students' confidence in job seeking. Originality/value: This pioneering research integrates a career management program into a business module, addressing employability challenges in Industry 4.0. It provides empirical evidence of the program's positive impact on students' perceived employability.

Lexical alignment, a communication phenomenon where conversational partners adapt their word choices to become more similar, plays an important role in the development of language and social communication skills. While this has been studied extensively in the conversations of preschool-aged children and their parents in Western, Educated, Industrialized, Rich, and Democratic (WEIRD) communities, research in other pediatric populations is sparse. This study makes significant expansions on the existing literature by focusing on alignment in naturalistic conversations of school-aged children from a non-WEIRD population across multiple conversational tasks and with different types of adult partners. Typically developing children aged 5 to 8 years (n = 45) engaged in four semi-structured conversations that differed by task (problem-solving vs. play-based) and by partner (parent vs. university student), resulting in a corpus of 180 conversations. Lexical alignment scores were calculated and compared to sham conversations, representing alignment occurring at the level of chance. Both children and adults coordinated their conversational utterances by re-using or aligning each other's word choices. This alignment behavior persisted across conversational tasks and partners, although the degree of alignment was moderated by the conversational context. These findings suggest that lexical alignment is a robust phenomenon in conversations between school-age children and adults. Furthermore, this study extends lexical alignment findings to a non-WEIRD culture, suggesting that alignment may be a coordination strategy employed by adults and children across diverse linguistic and cultural backgrounds.

Background: Methodological work contributes to improve the teaching-educational process, making it efficient and effective; and in the achievement of this purpose a useful form is the methodological instructional class, for which the use of guides could improve the quality of its development. Objective: To design and validate a guide for the instructive methodological class about the workshop class structure in the teaching staff of Prosthetic Rehabilitation II subject of the Guantanamo University of Medical Sciences. Methodology: A literature review was conducted about the methodological work through technology development. A guide for the development of the instructive methodological class was designed and validated for the teaching staff about the workshop class structure in topic III of Prosthetic Rehabilitation II subject. Theoretical and empirical methods were used for the research. Results: The resulting guide contains the elements related to the workshop class generalities as an organization form of teaching and its structure with the elements introduction, development and conclusions. Emphasis was given to argumentation, as well as to the analysis of the main methodological problems that may appear in the workshop class and their possible solutions. The experts validated the proposal, all criteria surpassed 4 points. Conclusions: The guide for the instructional methodological class designed and validated by the experts contains the argumentation, analysis and solutions of the methodological problems that the teaching staff may present in the workshop class, which contributes to the methodological work.

Background Digital impressions offering an alternative method that promises to enhance precision, reduce patient discomfort and streamline workflows. However, it is essential to compare the accuracy of both partial and complete arch impressions using conventional and digital techniques to provide evidence-based data that can guide dental professionals in selecting the most accurate and reliable method for different clinical scenarios. Ultimately, the goal is that these factors will lead to improved patient outcomes and advancing dental practices.Objectives To evaluate the accuracy of partial and complete arch impressions using conventional and digital techniques through an in-vitro 3D analysis.Materials and Methods Typodonts with zirconia crown preparation on tooth #19 along with grooves were prepared for alignment. Partial (PAS) and full (FAS) arch digital impressions were obtained using the intraoral scanner. Partial (PAC) and full (FAC) arch conventional impressions were made with vinyl polysiloxane (VPS) impression material, processed into a gypsum study cast and scanned with an intraoral scanner. A total of 120 STL files were superimposed and analyzed using three-dimensional analysis software.Results All experimental groups (PAS, FAC, and PAC) were statistically different from the control group (FAS). PAS demonstrated the least deviation (10.33 ± 29.00 μm) while PAC demonstrated the highest deviation (125.2 ± 81.88 μm) (Repeated ANOVA test, p

The severe impact of global warming, especially in the arctic region, have a multitude of consequences spanning from sea-level rises and freshening of the ocean, to significant changes to the animal life, biodiversity and species distribution. As the arctic regions are inherently remote and can be both hazardous and difficult to reach, research to improve our understanding of the climate change impact is often limited to short term field-campaigns. Here we present the Danish DISCO-2 student CubeSat mission, designed to meet the growing need for an Earth-observing platform. This mission leverages the rapid advancements in CubeSat technology over the past decades to overcome the limitations of traditional fieldwork campaigns. DISCO-2 will assist on-going arctic climate research with a payload of optical and thermal cameras in combination with novel in-orbit data analysis capabilities. It will further be capable of performing photogrammetric observations to determine ice volumes from deteriorating glaciers and provide surface temperatures, enabling studies of heat transfer between glaciers and arctic fjords. As a student satellite, the payload capabilities will also be offered to novel student research ideas throughout the mission life time. The modularity and wide range of of-the-shelf-components for CubeSats has facilitated an immense opportunity to tailor this earth observing CubeSat to accommodate specific scientific goals and further provided students at the participating universities with an unparalleled possibility to go from an initial research idea to a running CubeSat mission.

Bacterial small RNAs (sRNAs) play crucial roles in coordinating gene regulatory networks in various physiological processes, including biofilm formation. In this study, RNA sequencing was performed on biofilm (n = 4) and planktonic (n = 4) cells harvested at 10 h (pre-stationary phase of biofilm development) to identify biofilm-associated sRNAs in human methicillin-susceptible Staphylococcus aureus (MSSA) recovered from urine isolate. A total of 56 highly expressed sRNAs were identified with 15 overlapping sRNA genes (srn_9348, sprD, sRNA205, sRNA288, srn_2467, Sau-25, srn_2468, sRNA260, sRNA200, RsaE, sRNA397, Teg55, Teg60, RsaX05 and Teg140). Further validation through RT-qPCR analysis of nine sRNAs revealed that srn_9348 and sRNA260 were significantly expressed in the biofilm cells of urine sample. Both sRNAs were predicted to interact with mRNA genes including intracellular adhesin A (icaA) and host factor protein (hfq) involved in biofilm formation via cis-acting and trans-acting using CopraRNA analysis. Therefore, both sRNAs merit further investigations via reverse genetic approaches to elucidate their mechanism of translational regulation. In summary, the transcriptomic analysis conducted in this study offers new insights into the potential regulatory roles of sRNAs in MSSA biofilm development within the urinary environment.

BackgroundSmoking contributes to 1 in 3 cancer deaths. At the Stanford Cancer Center, tobacco cessation medication management and counseling are provided as a covered benefit. Patients charted as using tobacco are contacted by a tobacco treatment specialist and offered cessation services. As a novel addition, this study examined the acceptability of a virtual reality (VR) mindful exposure therapy app for quitting smoking called MindCotine. ObjectiveThe objective of this study was to determine the feasibility and acceptability of offering 6 weeks of MindCotine treatment as a part of Stanford’s Tobacco Treatment Services for patients seen for cancer care. MethodsAs part of a single-group pilot study, the MindCotine VR program was offered to English- or Spanish-speaking patients interested in quitting smoking. Given the visual interface, epilepsy was a medical exclusion. Viewed from a smartphone with an attachable VR headset, MindCotine provides a digital environment with audiovisual content guiding mindfulness exercises (eg, breathing techniques, body awareness, and thought recognition), text-based coaching, and cognitive behavioral therapy-based self-reflections for quitting smoking. Interested patients providing informed consent were mailed a MindCotine headset and asked to use the app for 10+ minutes a day. At the end of 6 weeks, participants completed a feedback survey. ResultsOf the 357 patients reached by the tobacco treatment specialist, 62 (17.3%) were ineligible, 190 (53.2%) were not interested in tobacco treatment services, and 78 (21.8%) preferred other tobacco treatment services. Among the 105 eligible and interested in assistance with quitting, 27 (25.7%) were interested in MindCotine, of whom 20 completed the informed consent, 9 used the program, and 8 completed their end-of-treatment survey. Participants using MindCotine completed, on average, 13 (SD 20.2) program activities, 19 (SD 26) journal records, and 11 (SD 12.3) coaching engagements. Of the 9 participants who used MindCotine, 4 (44%) reported some dizziness with app use that resolved and 7 (78%) would recommend MindCotine to a friend. In total, 2 participants quit tobacco (22.2% reporting, 10% overall), 2 others reduced their smoking by 50% or more, and 2 quit for 24 hours and then relapsed. ConclusionsIn a feasibility and acceptability pilot study of a novel VR tobacco treatment app offered to patients at a cancer center, 4 of 9 (44%) reporting and 4 of 20 (20%) overall substantially reduced or quit using tobacco after 6 weeks and most would recommend the app to others. Further testing on a larger sample is warranted. Trial RegistrationClinicalTrials.gov NCT05220254; https://clinicaltrials.gov/study/NCT05220254

Introduction Reducing neonatal deaths in premature infants in low- and middle-income countries is key to reducing global neonatal mortality. International neonatal networks, along with patient registries of premature infants, have contributed to improving the quality of neonatal care; however, the involvement of low-to-middle-income countries was limited. This project aims to form an international collaboration among neonatal networks in Asia (AsianNeo), including low-, middle- and high-income countries (or regions). Specifically, it aims to determine outcomes in sick newborn infants, especially very low birth weight (VLBW) infants or very preterm infants, with a view to improving the quality of care for such infants.Methods and analysis Currently, AsianNeo comprises nine neonatal networks from Indonesia, Japan, Malaysia, Philippines, Singapore, South Korea, Sri Lanka, Taiwan and Thailand. AsianNeo will undertake the following four studies: (1) institutional questionnaire surveys investigating neonatal intensive care unit resources and the clinical management of sick newborn infants, with a focus on VLBW infants (nine countries/regions); (2) a retrospective cohort study to describe and compare the outcomes of VLBW infants among Asian countries and regions (four countries/regions); (3) a prospective cohort study to develop the AsianNeo registry of VLBW infants (six countries/regions); and (4) implementation and evaluation of educational and quality improvement projects in AsianNeo countries and regions (nine countries/regions).Ethics and dissemination The study protocol was approved by the Research Ethics Board of the National Center for Child Health and Development, Tokyo, Japan (reference number 2020–244, 2022–156). The study findings will be disseminated through educational programmes, quality improvement activities, conference presentations and medical journal publications.

Introducción: el Sistema Nacional de Salud cubano cuenta con una red de revistas científicas médicas y dentro de estas las estudiantiles. Entender la historia de una revista científica ayuda a los investigadores a identificar publicaciones relevantes, seguir la evolución de ciertos temas o líneas de investigación, así como medir el impacto de la misma. Objetivo: describir la reseña histórica de la revista Gaceta Médica Estudiantil de la Universidad de Ciencias Médicas de Guantánamo, Cuba. Métodos: se realizó una investigación de corte histórico, a través de una revision documental, entrevista a expertos y galenos fundadores, así como la revisión de los diferentres registros, bases de datos en que ha sido registrada Gaceta Médica Estudiantil. Desarrollo: se relatan cronológicamente aspectos del surgimiento, fortalecimiento y consolidación de Gaceta Médica Estudiantil. Se resaltan sus principales resultados para la comunidad científica, tales como: Premio Rector 2023 de la Universidad de Ciencias Médicas de Guantánamo, Premio CITMA Guantánamo 2023 y se encuentra indexada en el Grupo 2, según lo establece la clasificación de revistas del Ministerio de Educación Superior. Conclusiones: la fundación de Gaceta Médica Estudiantil es un hito para la historia de la ciencia joven guantanamera. Pese a su corto tiempo contó con la dirección y asesorías correctas para su evolución. Este constituye el primer documento que aborda la historia de esta revista científica estudiantil.