19 results on '"A. C. Jenke"'
Search Results
2. The Dresden Surgical Anatomy Dataset for Abdominal Organ Segmentation in Surgical Data Science
- Author
-
Matthias Carstens, Franziska M. Rinner, Sebastian Bodenstedt, Alexander C. Jenke, Jürgen Weitz, Marius Distler, Stefanie Speidel, and Fiona R. Kolbinger
- Subjects
Science - Abstract
Measurement(s) Laparoscopic Surgery Technology Type(s) Laparoscopy Factor Type(s) Presence and location of anatomical structures within laparoscopic images Sample Characteristic - Organism Homo sapiens Sample Characteristic - Environment abdomen Sample Characteristic - Location abdominal cavity
- Published
- 2023
- Full Text
- View/download PDF
3. Human astrovirus infection associated with encephalitis in an immunocompetent child: a case report
- Author
-
Georgia Koukou, Sandra Niendorf, Britt Hornei, Jan-U Schlump, Andreas C. Jenke, and Sonja Jacobsen
- Subjects
Classic human astroviruses ,CNS infections ,Encephalitis ,Immunocompetent ,Gastroenteritis ,Medicine - Abstract
Abstract Background Until today, classic human astroviruses have not been associated with central nervous system infections in immunocompetent patients. Case presentation A 16-month-old Caucasian girl presented with repetitive generalized seizures with a 4-day history of watery diarrhea, which had already gradually improved. Initially, the prolonged seizures ceased after systemic midazolam treatment and were thought to be fever associated. However, her mental status remained altered, and after seizure recurrence, she was transferred to our pediatric intensive care unit. Seizure control was achieved by a combination of high-dose levetiracetam and phenobarbital, but she remained unconscious. An electroencephalogram at this time revealed generalized high voltage theta activity. All laboratory analyses, including extended blood and cerebrospinal fluid analyses, and a brain magnetic resonance imaging were normal. On day 4, the child gradually became conscious, but was very agitated and not able to walk. Since an electroencephalogram at this time still revealed generalized high voltage theta activity, although she had not received sedative medications for 72 hours, she was diagnosed as having encephalopathy. At that time, results of diagnostic testing of the stool sample were positive for classic astrovirus infection, and we decided to analyze the initially obtained cerebrospinal fluid for astrovirus as well. Cerebrospinal fluid was also found positive for human astrovirus. Sequencing analysis revealed a classic astrovirus genotype 1 with exactly the same nucleotide sequence as in the feces. Clinically, the child gradually improved and was discharged on day 9. Conclusions Whereas the new human astrovirus subtypes have been recently associated with central nervous system infection, this is the first case of encephalitis in an immunocompetent child due to classic human astrovirus. Considering that classic human astroviruses are the third most common etiological agents of viral gastroenteritis in children, we believe that human astroviruses as causative agents for central nervous system infections should be considered more often, especially in children and infants with preceding gastroenteritis.
- Published
- 2019
- Full Text
- View/download PDF
4. Neonatal exposure to hyperoxia leads to persistent disturbances in pulmonary histone signatures associated with NOS3 and STAT3 in a mouse model
- Author
-
Cho-Ming Chao, Rhea van den Bruck, Samantha Lork, Janica Merkle, Laura Krampen, Patrick P Weil, Malik Aydin, Saverio Bellusci, Andreas C. Jenke, and Jan Postberg
- Subjects
Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background Early pulmonary oxygen exposure is one of the most important factors implicated in the development of bronchopulmonary dysplasia (BPD). Methods Here, we analyzed short- and long-term effects of neonatal hyperoxia on NOS3 and STAT3 expression and corresponding epigenetic signatures using a hyperoxia-based mouse model of BPD. Results Early hyperoxia exposure led to a significant increase in NOS3 (median fold change × 2.37, IQR 1.54–3.68) and STAT3 (median fold change × 2.83, IQR 2.21–3.88) mRNA levels in pulmonary endothelial cells with corresponding changes in histone modification patterns such as H2aZac and H3K9ac hyperacetylation at the respective gene loci. No complete restoration in histone signatures at these loci was observed, and responsivity to later hyperoxia was altered in mouse lungs. In vitro, histone signatures in human aortic endothelial cells (HAEC) remained altered for several weeks after an initial long-term exposure to trichostatin A. This was associated with a substantial increase in baseline eNOS (median 27.2, IQR 22.3–35.6) and STAT3α (median 5.8, IQR 4.8–7.3) mRNA levels with a subsequent significant reduction in eNOS expression upon exposure to hypoxia. Conclusions Early hyperoxia induced permanent changes in histones signatures at the NOS3 and STAT3 gene locus might partly explain the altered vascular response patterns in children with BPD.
- Published
- 2018
- Full Text
- View/download PDF
5. Surgomics: personalized prediction of morbidity, mortality and long-term outcome in surgery using machine learning on multimodal data
- Author
-
Martin Wagner, Johanna M. Brandenburg, Sebastian Bodenstedt, André Schulze, Alexander C. Jenke, Antonia Stern, Marie T. J. Daum, Lars Mündermann, Fiona R. Kolbinger, Nithya Bhasker, Gerd Schneider, Grit Krause-Jüttler, Hisham Alwanni, Fleur Fritz-Kebede, Oliver Burgert, Dirk Wilhelm, Johannes Fallert, Felix Nickel, Lena Maier-Hein, Martin Dugas, Marius Distler, Jürgen Weitz, Beat-Peter Müller-Stich, and Stefanie Speidel
- Subjects
Machine Learning ,Surgeons ,Humans ,Surgery ,Morbidity - Abstract
Background Personalized medicine requires the integration and analysis of vast amounts of patient data to realize individualized care. With Surgomics, we aim to facilitate personalized therapy recommendations in surgery by integration of intraoperative surgical data and their analysis with machine learning methods to leverage the potential of this data in analogy to Radiomics and Genomics. Methods We defined Surgomics as the entirety of surgomic features that are process characteristics of a surgical procedure automatically derived from multimodal intraoperative data to quantify processes in the operating room. In a multidisciplinary team we discussed potential data sources like endoscopic videos, vital sign monitoring, medical devices and instruments and respective surgomic features. Subsequently, an online questionnaire was sent to experts from surgery and (computer) science at multiple centers for rating the features’ clinical relevance and technical feasibility. Results In total, 52 surgomic features were identified and assigned to eight feature categories. Based on the expert survey (n = 66 participants) the feature category with the highest clinical relevance as rated by surgeons was “surgical skill and quality of performance” for morbidity and mortality (9.0 ± 1.3 on a numerical rating scale from 1 to 10) as well as for long-term (oncological) outcome (8.2 ± 1.8). The feature category with the highest feasibility to be automatically extracted as rated by (computer) scientists was “Instrument” (8.5 ± 1.7). Among the surgomic features ranked as most relevant in their respective category were “intraoperative adverse events”, “action performed with instruments”, “vital sign monitoring”, and “difficulty of surgery”. Conclusion Surgomics is a promising concept for the analysis of intraoperative data. Surgomics may be used together with preoperative features from clinical data and Radiomics to predict postoperative morbidity, mortality and long-term outcome, as well as to provide tailored feedback for surgeons. Graphical abstract
- Published
- 2022
6. Speckle Tracking Stress Echocardiography Uncovers Early Subclinical Cardiac Involvement in Pediatric Patients with Inflammatory Bowel Diseases
- Author
-
Kai O. Hensel, Francisca E. Abellan Schneyder, Lucia Wilke, Andreas Heusch, Stefan Wirth, and Andreas C. Jenke
- Subjects
Medicine ,Science - Abstract
Abstract Inflammatory bowel disease (IBD) is an established risk factor for cardiovascular disease (CVD). However, whether cardiac consequences present early in IBD is unknown. This is the first study in children aiming to unmask altered myocardial mechanics in IBD. We enrolled 50 consecutive normotensive children with Crohn’s disease (CD) (n = 28) or ulcerative colitis (UC) (n = 22). The study groups consisted of 18 patients with active inflammatory disease (mean age 14.6 ± 2.5 years) and 32 children with IBD in remission (14.3 ± 2.3 years). 60 age- and gender-matched children served as healthy controls. Speckle tracking stress echocardiography (STE) was used to assess left ventricular (LV) myocardial strain and strain rate. Circumferential strain rate was significantly decreased in children with active IBD (−1.55 ± 0.26 s−1) and IBD in remission (−1.49 ± 0.26 s−1) versus healthy controls (1.8 ± 0.4 s−1) both at rest (p
- Published
- 2017
- Full Text
- View/download PDF
7. Anatomy Segmentation in Laparoscopic Surgery: Comparison of Machine Learning and Human Expertise
- Author
-
Fiona R. Kolbinger, Franziska M. Rinner, Alexander C. Jenke, Matthias Carstens, Stefanie Krell, Stefan Leger, Marius Distler, Jürgen Weitz, Stefanie Speidel, and Sebastian Bodenstedt
- Abstract
StructuredAbstractBackgroundLack of anatomy recognition represents a clinically relevant risk in abdominal surgery. Machine learning (ML) methods can help identify visible patterns and risk structures, however, their practical value remains largely unclear.Materials and MethodsBased on a novel dataset of 13195 laparoscopic images with pixel-wise segmentations of eleven anatomical structures, we developed specialized segmentation models for each structure and combined models for all anatomical structures using two state-of-the-art model architectures (DeepLabv3 and SegFormer), and compared segmentation performance of algorithms to a cohort of 28 physicians, medical students, and medical laypersons using the example of pancreas segmentation.ResultsMean Intersection-over-Union for semantic segmentation of intraabdominal structures ranged from 0.28 to 0.83 and from 0.23 to 0.77 for the DeepLabv3-based structure-specific and combined models, and from 0.31 to 0.85 and from 0.26 to 0.67 for the SegFormer-based structure-specific and combined models, respectively. Both the structure-specific and the combined DeepLabv3-based models are capable of near-real-time operation, while the SegFormer-based models are not. All four models outperformed at least 26 out of 28 human participants in pancreas segmentation.ConclusionsThese results demonstrate that ML methods have the potential to provide relevant assistance in anatomy recognition in minimally-invasive surgery in near-real-time. Future research should investigate the educational value and subsequent clinical impact of respective assistance systems.HighlightsMachine learning models to reduce surgical risks that precisely identify 11 anatomical structures: abdominal wall, colon, intestinal vessels (inferior mesenteric artery and inferior mesenteric vein with their subsidiary vessels), liver, pancreas, small intestine, spleen, stomach, ureter and vesicular glandsLarge training dataset of 13195 real-world laparoscopic images with high-quality anatomy annotationsSimilar performance of individual segmentation models for each structure and combined segmentation models in identifying intraabdominal structures, and similar segmentation performance of DeepLabv3-based and SegFormer-based modelsDeepLabv3-based models are capable of near-real-time operation while SegFormer-based models are not, but SegFormer-based models outperform DeepLabv3-based models in terms of accuracy and generalizabilityAll models outperformed at least 26 out of 28 human participants in pancreas segmentation, demonstrating their potential for real-time assistance in recognizing anatomical landmarks during minimally-invasive surgery.
- Published
- 2022
8. Subclinical Alterations of Cardiac Mechanics Present Early in the Course of Pediatric Type 1 Diabetes Mellitus: A Prospective Blinded Speckle Tracking Stress Echocardiography Study
- Author
-
Kai O. Hensel, Franziska Grimmer, Markus Roskopf, Andreas C. Jenke, Stefan Wirth, and Andreas Heusch
- Subjects
Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Diabetic cardiomyopathy substantially accounts for mortality in diabetes mellitus. The pathophysiological mechanism underlying diabetes-associated nonischemic heart failure is poorly understood and clinical data on myocardial mechanics in early stages of diabetes are lacking. In this study we utilize speckle tracking echocardiography combined with physical stress testing in order to evaluate whether left ventricular (LV) myocardial performance is altered early in the course of uncomplicated type 1 diabetes mellitus (T1DM). 40 consecutive asymptomatic normotensive children and adolescents with T1DM (mean age 11.5±3.1 years and mean disease duration 4.3±3.5 years) and 44 age- and gender-matched healthy controls were assessed using conventional and quantitative echocardiography (strain and strain rate) during bicycle ergometer stress testing. Strikingly, T1DM patients had increased LV longitudinal (p=0.019) and circumferential (p=0.016) strain rate both at rest and during exercise (p=0.021). This was more pronounced in T1DM patients with a longer disease duration (p=0.038). T1DM patients with serum HbA1c>9% showed impaired longitudinal (p=0.008) and circumferential strain (p=0.005) and a reduced E/A-ratio (p=0.018). In conclusion, asymptomatic T1DM patients have signs of hyperdynamic LV contractility early in the course of the disease. Moreover, poor glycemic control is associated with early subclinical LV systolic and diastolic impairment.
- Published
- 2016
- Full Text
- View/download PDF
9. Stay Focused - Enhancing Model Interpretability Through Guided Feature Training
- Author
-
Alexander C. Jenke, Sebastian Bodenstedt, Martin Wagner, Johanna M. Brandenburg, Antonia Stern, Lars Mündermann, Marius Distler, Jürgen Weitz, Beat P. Müller-Stich, and Stefanie Speidel
- Published
- 2022
10. DNA methylation analysis in the intestinal epithelium-effect of cell separation on gene expression and methylation profile.
- Author
-
Andreas C Jenke, Jan Postberg, Timothy Raine, Komal M Nayak, Malte Molitor, Stefan Wirth, Arthur Kaser, Miles Parkes, Robert B Heuschkel, Valerie Orth, and Matthias Zilbauer
- Subjects
Medicine ,Science - Abstract
Epigenetic signatures are highly cell type specific. Separation of distinct cell populations is therefore desirable for all epigenetic studies. However, to date little information is available on whether separation protocols might influence epigenetic and/or gene expression signatures and hence might be less beneficial. We investigated the influence of two frequently used protocols to isolate intestinal epithelium cells (IECs) from 6 healthy individuals.Epithelial cells were isolated from small bowel (i.e. terminal ileum) biopsies using EDTA/DTT and enzymatic release followed by magnetic bead sorting via EPCAM labeled microbeads. Effects on gene/mRNA expression were analyzed using a real time PCR based expression array. DNA methylation was assessed by pyrosequencing of bisulfite converted DNA and methylated DNA immunoprecipitation (MeDIP).While cell purity was >95% using both cell separation approaches, gene expression analysis revealed significantly higher mRNA levels of several inflammatory genes in EDTA/DTT when compared to enzymatically released cells. In contrast, DNA methylation of selected genes was less variable and only revealed subtle differences. Comparison of DNA methylation of the epithelial cell marker EPCAM in unseparated whole biopsy samples with separated epithelium (i.e. EPCAM positive and negative fraction) demonstrated significant differences in DNA methylation between all three tissue fractions indicating cell type specific methylation patterns can be masked in unseparated tissue samples.Taken together, our data highlight the importance of considering the potential effect of cell separation on gene expression as well as DNA methylation signatures. The decision to separate tissue samples will therefore depend on study design and specific separation protocols.
- Published
- 2013
- Full Text
- View/download PDF
11. Flow rate influencing effects of micropumps
- Author
-
S. Kager, C. Jenke, Christoph Kutter, M. Richter, and Publica
- Subjects
010302 applied physics ,Materials science ,Degasser ,Capacitive sensing ,Flow (psychology) ,Metals and Alloys ,Micropump ,02 engineering and technology ,Mechanics ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Volumetric flow rate ,Volume (thermodynamics) ,Cavitation ,0103 physical sciences ,Electrical and Electronic Engineering ,Current (fluid) ,0210 nano-technology ,Instrumentation - Abstract
Current micropump technology features bubble tolerance and self-priming ability, achieved with high pressure ability at shallow pump chambers for low dead volume and high compression ratio. Though, the pump dynamics are strongly affected by a pump chamber height dependent viscous squeeze film damping. Therefore, we investigated all major flow influencing effects for micro diaphragm pumps, being liquid resistive damping, cavitation based gaseous capacitive damping and reactive valve volumes. Analytical models were developed that are able to describe and predict the transient actuator displacement, derive the frequency dependent displacement and estimate the valve loss effect in order to determine the flow rate performance of micropumps. In addition, a new oscillator model is able to simulate the inception of cavitation and the time course of both actuator and liquid. The individual effect models were combined into a single-cycle based, frequency dependent, flow rate model, validated for frequencies of up to 100 Hz. Compared to the maximum achievable flow rate, the liquid resistive damping reduces dynamic stroke volume and is responsible for a reduction of 23-57 % at 100 Hz. Valve induced flow losses reduce the effectively redirected volume towards the outlet. The effect is enhanced by the strong damping, but also feeds back on the actuator by reducing initial damping significantly. Reactive valve volumes amount for 6.2-12.4% flow rate reduction at 100 Hz. Full cavitation, generated at the supply mode and affecting the pump mode, can cause gas bubble generation and lead to a drop of flow rate down to 65% at 80 Hz. By placing an in-line degasser the flow stability was improved to ±2.2% over 10 h. After reducing the frequency to 20 Hz, cavitation only affected the supply mode and in combination with a degasser a flow stability of ±0.15% over 2 h was achieved. By reducing voltage levels, cavitation was avoided completely, featuring a stability of ±0.08%. The understanding of the flow rate limiting effects enables the application specific design to meet all performance and reliability requirements for micropump based liquid dosing systems.
- Published
- 2018
12. The Effect of Physical Readiness Training on Reserve Officers' Training Corps Freshmen Cadets
- Author
-
Shane C. Jenke, Jason D. Stone, Andrew R. Jagim, Jonathan M. Oliver, Margaret T. Jones, and Chris Holt
- Subjects
Male ,medicine.medical_specialty ,Physical fitness ,0211 other engineering and technologies ,Squat ,02 engineering and technology ,Body Mass Index ,Young Adult ,03 medical and health sciences ,Vertical jump ,0302 clinical medicine ,medicine ,Humans ,Longitudinal Studies ,Exercise ,021110 strategic, defence & security studies ,business.industry ,Teaching ,Public Health, Environmental and Occupational Health ,VO2 max ,030229 sport sciences ,General Medicine ,Texas ,Test (assessment) ,Military Personnel ,Physical Fitness ,Body Composition ,Exercise Test ,Physical therapy ,Cadet ,Female ,business ,Early phase - Abstract
The U.S. Army Reserve Officers' Training Corps (ROTC) is the largest commissioning source among all branches. Physical Readiness Training (PRT) is fundamental to ROTC cadet training. However, although studies have evaluated the effectiveness of PRT in cadets with prior PRT experience, no study has examined the early phase adaptations in those cadets with no prior PRT experience.To determine the effect of PRT over the course of an academic year (9 months) in freshmen ROTC cadets, thirteen (n = 13; 7 male [180.8 ± 4.7 cm, 77.4 ± 10.0 kg; 19.8 ± 6.5 % body fat], 6 female [159.5 ± 12.1 cm; 63.4 ± 8.4 kg; 29.6 ± 6.2% body fat]) cadets with no prior PRT performed laboratory (body composition, 1-repetition maximum [1RM] bench and squat, countermovement vertical jump, maximal aerobic capacity [VONo changes occurred in body composition, VONine months of PRT improved APFT scores of freshmen cadets while minor effects were noted in laboratory-based performance. Given the lack of improvements in strength and power, it would be advisable to provide supplemental strength and power training. Being a large commissioning resource for the Army with greater access to equipment and resources, the time spent in ROTC may be a unique opportunity to improve and educate young commissioning officers on the benefits of proper strength and power training for the use in future commands while units are considerably smaller and equipment is more accessible on university grounds.
- Published
- 2017
13. Acute Effect of Cluster and Traditional Set Configurations on Myokines Associated with Hypertrophy
- Author
-
Shane C. Jenke, Jonathan M. Oliver, Margaret T. Jones, John D. Mata, and Andreas Kreutzer
- Subjects
Adult ,Male ,medicine.medical_specialty ,Physical Therapy, Sports Therapy and Rehabilitation ,Acute effect ,Squat ,Muscle hypertrophy ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Myokine ,medicine ,Humans ,Orthopedics and Sports Medicine ,030212 general & internal medicine ,Muscle, Skeletal ,Mathematics ,Interleukin-15 ,Cross-Over Studies ,Interleukin-6 ,Resistance Training ,Hypertrophy ,030229 sport sciences ,Venous blood ,Crossover study ,Confidence interval ,Biomechanical Phenomena ,Cytokine response ,Cardiology ,Physical therapy - Abstract
This study compared the acute cytokine response, and kinetic and kinematic profile following back squat exercise in resistance-trained men. In a randomized, cross-over design, 10 resistance-trained men (27±4 y, 1.80±0.07 m, 82.8±6.7 kg, 16.3±3.5% fat) performed the back squat exercise using traditional and cluster set configurations. Kinetic and kinematic data were sampled throughout each condition. Venous blood was sampled prior, immediately post, 30 min, 60 min, 24 h, and 48 h post-exercise for plasma interleukin-6 (IL-6) and interleukin-15 (IL-15). Cluster sets allowed for greater mean power (mean difference, 110 W; 90% confidence interval, ±63 W; benefit odds, 41 447:1), driven by higher overall mean velocities (0.053 m∙s−1; 0.039 m∙s−1; 3 105:1) as evidenced by the lack of clear contrasts for mean force. IL-15 increased post-exercise in both conditions, but increased at 24 h (0.13 pg·mL−1; ±0.11 pg·mL−1; 486:1) and 48 h (0.12 pg·mL−1; ±0.10 pg·mL−1; 667:1) in traditional sets only. IL-6 increased similarly in both conditions, post-exercise through 60 min post. Cluster set configurations allow for greater mean power, attributed to higher velocities. Despite a similar response of IL-6, traditional set configuration may provide a greater stimulus for hypertrophy as evidenced by a secondary increase in IL-15.
- Published
- 2016
14. Velocity Drives Greater Power Observed During Back Squat Using Cluster Sets
- Author
-
Jonathan M. Oliver, Melody D. Phillips, Shane C. Jenke, Margaret T. Jones, Andreas Kreutzer, and Joel B. Mitchell
- Subjects
Adult ,Male ,Rest ,Posture ,Physical Therapy, Sports Therapy and Rehabilitation ,Squat ,Kinematics ,03 medical and health sciences ,0302 clinical medicine ,One-repetition maximum ,Statistics ,Humans ,Orthopedics and Sports Medicine ,Power output ,Exercise ,Mathematics ,Tension (physics) ,Resistance training ,Resistance Training ,030229 sport sciences ,General Medicine ,Adaptation, Physiological ,Biomechanical Phenomena ,Power (physics) ,Volume load ,Kinetics ,030217 neurology & neurosurgery - Abstract
This investigation compared the kinetics and kinematics of cluster sets (CLU) and traditional sets (TRD) during back squat in trained (RT) and untrained (UT) men. Twenty-four participants (RT = 12, 25 ± 1 year, 179.1 ± 2.2 cm, 84.6 ± 2.1 kg; UT = 12, 25 ± 1 year, 180.1 ± 1.8 cm, 85.4 ± 3.8 kg) performed TRD (4 × 10, 120-second rest) and CLU (4 × (2 × 5) 30 seconds between clusters; 90 seconds between sets) with 70% one repetition maximum, randomly. Kinematics and kinetics were sampled through force plate and linear position transducers. Resistance-trained produced greater overall force, velocity, and power; however, similar patterns were observed in all variables when comparing conditions. Cluster sets produced significantly greater force in isolated repetitions in sets 1-3, while consistently producing greater force due to a required reduction in load during set 4 resulting in greater total volume load (CLU, 3302.4 ± 102.7 kg; TRD, 3274.8 ± 102.8 kg). Velocity loss was lessened in CLU resulting in significantly higher velocities in sets 2 through 4. Furthermore, higher velocities were produced by CLU during later repetitions of each set. Cluster sets produced greater power output for an increasing number of repetitions in each set (set 1, 5 repetitions; sets 2 and 3, 6 repetitions; set 4, 8 repetitions), and the difference between conditions increased over subsequent sets. Time under tension increased over each set and was greater in TRD. This study demonstrates greater power output is driven by greater velocity when back squatting during CLU; therefore, velocity may be a useful measure by which to assess power.
- Published
- 2016
15. Acute response to cluster sets in trained and untrained men
- Author
-
Shane C. Jenke, Joel B. Mitchell, Andreas Kreutzer, Jonathan M. Oliver, Margaret T. Jones, and Melody D. Phillips
- Subjects
Gerontology ,Adult ,Male ,medicine.medical_specialty ,Anabolism ,Hydrocortisone ,Weight Lifting ,Physiology ,Squat ,Growth hormone ,Young Adult ,Physiology (medical) ,Internal medicine ,Blood lactate ,Medicine ,Humans ,Orthopedics and Sports Medicine ,Testosterone ,Metabolic Stress ,Lactic Acid ,Muscle, Skeletal ,business.industry ,Human Growth Hormone ,Public Health, Environmental and Occupational Health ,Resistance Training ,General Medicine ,Volume load ,Endocrinology ,business ,Hormonal response - Abstract
In traditional sets (TRD) repetitions are performed continuously, whereas cluster sets (CLU) allow a brief rest between groups of repetitions. We investigated the acute mechanical, metabolic, and hormonal response to CLU in men. Twelve resistance-trained (RT) and 11 untrained (UT) men performed TRD (4 × 10 repetitions with 2 min rest) and CLU [4 × (2 × 5) with 1.5 min rest between sets 30 s rest between clusters] at 70 % 1RM back squat in random order. Seven days separated trials. Average power and time under tension (TUT) were calculated. Blood was sampled pre, sets 1, 2, and 3; immediate post-exercise, 5, 15, 30, 60 min post-exercise for blood lactate, total testosterone (TT), free testosterone (FT), growth hormone (GH), and cortisol. CLU produced greater average power at an increasing number of repetitions over each set with greater total volume load. TUT was shorter for RT and lower for CLU in repetitions 1, 6, 7, 8. Blood lactate was higher Set 2 through 30 min in TRD. RT had higher TT; however, the time course was similar between RT and UT. TT and FT increased immediate post-exercise and remained elevated 30 min in both conditions. GH was significantly greater during TRD with a similar pattern observed in both conditions. Cortisol was significantly lower at 30 min in CLU. CLU allowed greater total volume load, shorter TUT, greater average power, similar anabolic hormonal response, and less metabolic stress. The acute response was similar despite training status.
- Published
- 2014
16. Rotavirus vaccination: a risk factor for intussusception?
- Author
-
R. von Kries, Doris Oberle, Dirk Mentzer, Brigitte Keller-Stanislawski, and A. C. Jenke
- Subjects
Male ,Pediatrics ,medicine.medical_specialty ,First year of life ,Comorbidity ,medicine.disease_cause ,Rotavirus vaccination ,Mass Vaccination ,Rotavirus Infections ,Risk Factors ,Rotavirus ,Intussusception (medical disorder) ,Germany ,medicine ,Humans ,Risk factor ,business.industry ,Incidence (epidemiology) ,Incidence ,Public Health, Environmental and Occupational Health ,Infant, Newborn ,Rotavirus Vaccines ,Infant ,medicine.disease ,Vaccination ,Causality ,Treatment Outcome ,Child, Preschool ,Female ,business ,Intussusception - Abstract
Recently published pharmacoepidemiological studies associate the currently authorized Rotavirus (RV) vaccines with intussusception (IS). We aimed at investigating whether, in Germany, there are excess IS cases in RV vaccinees compared with the background incidence before market authorization in 2006. Suspected cases of IS following receipt of RV vaccines reported to the Paul-Ehrlich-Institut (PEI) from 2006 to 2010 were reviewed and validated against the criteria of the Brighton Collaboration's definition for IS. An observed-versus-expected analysis was conducted using standardized morbidity ratio (SMR) methods based on age-specific incidence rates for IS ranging from 19.2 to 98.5 per 100,000 person-years. A total of 27 cases of suspected IS in RV vaccinees were reported to the PEI. No excess of IS cases could be detected 1-7 days after receipt of either RV vaccine after any dose in the first year of life; however, in infants aged 3-5 months, a significantly increased SMR for IS was found in a risk window of 1-7 days after the first dose of either RV vaccine [SMRs: Rotarix® 4.6 (95% CI 1.5-10.7); RotaTeq® 5.8 (95% CI 1.2-17.1)]. A significantly increased risk of IS in a risk window of 1-7 days after RV vaccination was not found when the first dose was administered earlier. Therefore, it is recommended to start the vaccination course at 6-12 weeks of age.
- Published
- 2014
17. Incidence of intussusception in early infancy: a capture-recapture estimate for Germany
- Author
-
Andrea Streng, S. Weiß, A. C. Jenke, S. Wirth, Johannes G. Liese, and R. von Kries
- Subjects
Male ,Pediatrics ,medicine.medical_specialty ,Cross-sectional study ,Statistics as Topic ,medicine.disease_cause ,Vaccines, Attenuated ,Mark and recapture ,Rotavirus ,Intussusception (medical disorder) ,Germany ,Product Surveillance, Postmarketing ,Medicine ,Adverse Drug Reaction Reporting Systems ,Humans ,business.industry ,Drug Substitution ,Incidence (epidemiology) ,Incidence ,Age Factors ,Rotavirus Vaccines ,ICD-10 ,Infant ,medicine.disease ,Hospital Records ,Rotavirus vaccine ,Vaccination ,Cross-Sectional Studies ,Population Surveillance ,Pediatrics, Perinatology and Child Health ,Female ,business ,Intussusception - Abstract
BACKGROUND Rotavirus (RV) vaccination with the first generation vaccine (Rotashield) has been associated with intussusception (IS). Reliable age specific baseline incidence data for children ≤6 months of age in particular is fundamental for further post marketing monitoring of potential effects of recently introduced new RV vaccines. METHODS IS incidence was estimated by a 2-source capture-recapture calculation (CRC) based on ESPED (German Paediatric Surveillance Unit) reports and on hospital discharge records for 2006 and 2007. ESPED as well as hospital records were validated according to the Brighton Collaboration's Group definition for definite IS. PATIENTS Children that have been treated for IS in a hospital in one of two states of Germany (North-Rhine Westphalia and Bavaria). RESULTS The annual IS incidence for infants
- Published
- 2011
18. An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo
- Author
-
Frank O. Fackelmayer, Franziska Jönsson, Harald Conradt, Jürgen Bode, Bok Hee C Jenke, Hans J. Lipps, Christian Fetzer, Isa M. Stehle, and Institute of Cell Biology, Stockumer Strasse 10, University of Witten/Herdecke, D-58448 Witten.
- Subjects
Immunoprecipitation ,Blotting, Western ,Genetic Vectors ,CHO Cells ,Heterogeneous-Nuclear Ribonucleoprotein U ,Matrix (biology) ,Biology ,Origin of replication ,Heterogeneous ribonucleoprotein particle ,Biochemistry ,Heterogeneous-Nuclear Ribonucleoproteins ,Blotting, Southwestern ,Cricetinae ,Genetics ,Animals ,Molecular Biology ,Mitosis ,Ribonucleoprotein ,Chinese hamster ovary cell ,Scientific Reports ,Nuclear matrix ,Molecular biology ,Cell biology ,Ribonucleoproteins ,Cisplatin - Abstract
pEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintained in the absence of selection pressure. It has been suggested that mitotic stability is provided by a specific interaction of this vector with components of the nuclear matrix. We studied the interactions of pEPI-1 by crosslinking with cis-diamminedichloroplatinum II, after which it is found to copurify with the nuclear matrix. In a south-western analysis, the vector shows exclusive binding to hnRNP-U/SAF-A, a multifunctional scaffold/matrix specific factor. Immunoprecipitation of the crosslinked DNA-protein complex demonstrates that pEPI-1 is bound to this protein in vivo. These data provide the first experimental evidence for the binding of an artificial episome to a nuclear matrix protein in vivo and the basis for understanding the mitotic stability of this novel vector class.
- Published
- 2002
19. The effect of meal composition on postprandial glucagon-like peptide-1 response in overweight/obese participants
- Author
-
Joel B. Mitchell, Henry Aleck, Brian Franklin, Shane C. Jenke, Justin Repshas, Leighsa Brace, Brooke Bouza, Beverley Adams-Huet, Manall Jaffery, Lyn Dart, Alex Villanueva, Melody D. Phillips, Aaron R. Caldwell, Elizabeth Sanders, and Meena Shah
- Subjects
endocrine system ,medicine.medical_specialty ,Nutrition and Dietetics ,business.industry ,digestive, oral, and skin physiology ,Overweight obesity ,Clinical nutrition ,Overweight ,medicine.disease ,Glucagon-like peptide-1 ,Endocrinology ,Postprandial ,Diabetes mellitus ,Internal medicine ,Poster Presentation ,medicine ,Secretion ,Meal composition ,medicine.symptom ,business ,hormones, hormone substitutes, and hormone antagonists ,Food Science - Abstract
Background Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted in the intestine in response to food intake. GLP-1 may be responsible for nearly 50% of insulin secretion. Postprandial GLP-1 secretion may be impaired in overweight/obese (OW/O) individuals and in patients with type-2 diabetes (T2D). Meals high in protein (HP) or high in monounsaturated fat (HMF) may increase GLP-1 response. However, there are no studies directly comparing HP with HMF meals on postprandial GLP-1 response.
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.