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The article studies the influence of hymatomelanic acids, one of the fractions of humic substances of peloids, on the course of rheumatoid arthritis. This substance was standardized by using physicochemical analysis. The elemental composition of the substance as well as a number of spectral (IR, NMR) characteristics were revealed. The list of components formed as a result of hydrolysis of the initial preparation and their relative content was determined. Fatty acids and their derivatives are the predominant constituents of peloid hymatomelanic acid hydrolyzate. The tissues of immunocompetent organs including regional lymph nodes, spleen, and also rat liver were studied. For the experiment, a model of adjuvant arthritis was selected and four groups of animals were formed. They were withdrawn from the experiment on the 17, 21 and 26 day. The histological picture indicated that when modeling rheumatoid arthritis, such pathological changes in the immunocompetent organs of rats as edema of the parenchyma and stroma, sclerosis, thickening of the walls of blood vessels, develop during the whole period of observation. A list of descriptors of the studied drug was obtained and it was found that the use of peloid himatomelanic acids in the model of adjuvant arthritis had a pronounced anti-inflammatory effect, as evidenced by the normalization of the morphofunctional state of the spleen, regional lymph nodes and liver.

This article is devoted to the development of technology and methods for assessing the quality of suppositories containing humic acids of low-mineralized silt sulfide mud. The quality standards of the studied medicinal product were established and its stability during storage was determined. The quantitative determination of humic acids in aqueous solutions was determined by the spectrophotometric method. When studying the technological indicators of the suppositories, the following indicators were taken into account: description, average weight, melting temperature and time of complete deformation. The results of visual control showed that the appearance of the suppositories did not change during one year of storage. However, some white plaque and color heterogeneity appeared with the increase of the shelf life up to 1.5 years. The amount of the active substance during the storage of suppositories changed slightly and did not exceed the permissible content standards. The melting point value did not exceed 36 C, and its decrease was not observed. The time of complete deformation of the suppositories did not exceed 15 minutes and was 5-6 minutes during storage. The average weight of suppositories during storage remained stable. The study allows to conclude that the proposed suppositories are stable for 12 months and the preliminary shelf life of suppositories is 1 year. The conducted chemical and pharmaceutical studies scientifically substantiate the expediency of using medicines with humic acids that demonstrate antioxidant and anti-inflammatory properties. This will expand the range of domestic medicines with the given spectrum of therapeutic action.

In May and June 2018 and June 2019 we surveyed breeding populations of large raptors (principally Golden Eagle Aquila chrysaetos, Eastern Imperial Eagle Aquila heliaca, Steppe Eagle Aquila nipalensis, Long-Legged Buzzard Buteo rufinus, Saker Falcon Falco cherrug and Eagle Owl Bubo bubo) in the north-west of the Ustyurt Plateau within the boundaries of the Atyrau Province, Kazakhstan. The area surveyed was ca. 1000 km2 represented the wider area of 3400 km2. Total 12 species of birds of prey were recorded and 8 of them considered as large raptors. Golden Eagle was found nesting on the Donyztau escarpment, 4 breeding territories were recorded and 3 of them successful; nesting density is estimated at about 5.0–6.7 pairs per 100 km of cliffs, the distance between neighboring pairs was ca. 6 km. Imperial Eagle: 5 breeding territories found on the Donyztau and Zheltau escarpments, 4 of them successful; average nesting density is 3.8 pairs per 100 km of cliffs. Steppe Eagle: 14 breeding territories (25 nests) revealed, among them 6 are successful (1–2 nestlings) and on other 3 breeding was failed; average nesting density is estimated at 1.3 pairs per 100 km2. Average distance between neighboring active nests is 2.5 km in Sholkara hill ridge but the distance between successful nests here is much bigger and makes about 13 km. Long-Legged Buzzard: 13 breeding territories (30 nests) found, of which 11 territories were actually occupied and 8 of them had successful breeding. Saker Falcon is found nesting on Donyztau escarpment: 4 breeding territories, including 3 successful; the average nesting density is 6.7 pairs per 100 km of cliffs. Eagle Owl recorded throughout the surveyed area; 11 breeding territories revealed, the average nesting density is 7 breeding territories per 100 km of cliffs and steep slopes. Two other species, Short-toad Eagle and Cinereous Vulture, recorded only occasionally, nesting in the area is not found. In general, our findings demonstrate the area is important for conserving several species of large raptors in the Atyrau Province.

The article discusses the specifics of children's music on the example of the collection "Pieces for Kids" by Edison Denisov. The authors use the example of the analysis of the musical language of this collection to define the specifics of music for kids of the second half of the twentieth century in general and some features of the author's style of Denisov in particular. Despite the traditional nature of the chosen means of musical expression, based on the basic principles of tonal organization (functionality, connection between harmony and form creation, etc.), the pieces sound very fresh and modern. This explains the fact that the pieces in the collection are difficult to enter into the pedagogical repertoire. At the same time, the composer expands the tonality by means of various types of major-minor (of the same name, parallel, one-point), and also uses the means of chromatic tonality. Updating the musical language is associated with updating the chord. Denisov uses not only chords of the third structure, but also fourth, second chords and accords. The modal structure of the pieces is also diverse. Along with major and minor, the collection contains examples of using a natural diatonic modes.

One of the relevant tasks of current morphology is the investigation of tissue regenerative potential and research for new medications that improve recovery process efficiency. Nowadays, clinical specialists focus on medications that are based on natural compounds. However, such medication influence on the processes that occur within the injured tissues is still not identified. The purpose of this study was to investigate skeletal muscle tissue posttraumatic regeneration response to humic peloid medication, based on humic acids modified by Zinc ions. Humic acid extraction was carried out by means of patent procedure. The study included laboratory Wistar rats with hyperextension of front femur muscle. The preparations were studied by means of light and electronic microscopy and autoradiography. Histologic preparations evaluation showed that under peloid medication exposure the apolexis processes within muscle fibers rupture are inhibited, interstitial edema becomes restricted by the injured area, vessel growth into the area of damaged capillaries is stimulated, macrophages migrate and the area and duration of posttraumatic inflammation decrease. Additionally, peloid medication intake shortens the length of skeletal muscle tissue reparative histogenesis stages: myosatellitocytes are activated earlier than in the control group, myoblasts and myosymplasts are detected, the separation of nuclear sarcoplasmatic areas from partly injured muscle fibers is stimulated, myotubules appear 3 – 5 days earlier than in control group. Overall, muscle tissue regeneration efficiency increases by 21%. Obtained results allow us to conclude that peloid medication based on humic acids modified by Zinc ions positively influence the stimulation of the regeneration process. This will lead to further investigation of humic substances: fulvic, hymatomelanic, humin and humic acids of peloids as medications and their implementation in clinical practice.

We have applied the method of a forming tree-nesting behavior pattern in the chicks of the cliff-nesting Peregrine Falcon. In June 2016 and 2017, in the Southern Ural Mountains and Bugulma-Belebey Upland, we discovered four nests of Peregrine Falcons, which were threatened by destruction due to various anthropogenic and biological factors. For preventing the death of the broods, the chicks were transferred from the occupied nesting niches in the rock cliffs to nesting platforms. On nesting platforms they spent from 3 to 12 days where they were fed by adults regularly. All four broods (9 young) flew out successfully and demonstrated typical behavior for the Peregrines of their age. Adults fed fledglings and taught them to hunt.

The article characterizes the quantitative composition and forms of social activity of attorneys as representatives of the Samara intelligentsia at the beginning of the 20th century. Based on materials from the personal fund of the attorney at law A.G. Elshin in TsGASO reveals the main directions of his professional and social activities in the Samara province in 1905-1918.

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Humic acids restore not only normal physiological redox systems, but also increase the activity of antioxidant enzymes on the 10th day.Issledovali vliianie otdel'nykh komponentov gumusovykh kislot peloidov na protsessy svobodnoradikal'nogo okisleniia v modeli okislitel'nogo stressa, vyzvannogo u belykh besporodnykh krys polovozrelogo vozrasta deĭstviem polikhlorirovannykh bifenilov. Biologicheskuiu aktivnost' peloidopreparatov opredeliali po izmeneniiu obshcheĭ antioksidantnoĭ aktivnosti, aktivnosti superoksiddismutazy, katalazy i glutationperoksidazy na tret'i i desiatye sutki éksperimenta. Izmenenie aktivnosti fermentov pod deĭstviem peloidopreparatov podtverdilo, chto sostoianie okislitel'nogo stressa poddaetsia korrektsii uzhe na tret'i sutki éksperimenta. Na desiatye sutki guminovye kisloty ne tol'ko vosstanavlivaiut fiziologicheskuiu normu redoks-bufernykh sistem, no i povyshaiut aktivnost' antioksidantnykh fermentov.

A new method of testing restriction nucleases is proposed. This method is based on high-temperature treatment of crude cell extracts. Disrupted cells were heated at 50–60°C, centrifuged, and assayed for restrictases. This method provides the opportunity for screening new enzymes in microbial strains enriched with nonspecific restrictases. High-temperature treatment of cell extracts of certain producers reduces the number of steps of the procedure used for isolating high-purity restrictases; the resulting preparations are capable of maintaining high enzymatic activity during long-term storage. It was shown that high-temperature treatment can be applied not only to thermophilic but also to mesophilic strains of microorganisms of different taxa.

Original Russian-made preparation of human recombinant granulocytic colony-stimulating factor possessesin vitro activity similar to that of standard preparation, activatesin vivo production of humoral factors by adherent cells of the hemopoiesis-inducing microenvironment, and stimulates hemopoiesis in the bone marrow at various levels.

A new method of testing restriction nucleases is proposed. This method is based on high-temperature treatment of crude cell extracts. Disrupted cells were heated at 50-60 degrees C, centrifuged, and assayed for restrictases. This method provides the opportunity for screening new enzymes in microbial strains enriched with nonspecific restrictases. High-temperature treatment of cell extracts of certain producers reduces the number of steps of the procedure used for isolating high-purity restrictases; the resulting preparations are capable of maintaining high enzymatic activity during long-term storage. It was shown that high-temperature treatment can be applied not only to thermophilic but also to mesophilic strains of microorganisms of different taxa.

The paper deals with the development and design of a kit for choosing samples from various objects for microbiological studies. The studies clarified the composition of the set and requirements for the complete set and its parts. The design of this set parts was made and the design documents were drawn up for manufacturing the sampling set. A programme and procedures for microbiological and pilot tests of the kit were developed and its trials were performed.