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Development of productive strawberry cultivars adapted to harsh winter conditions is an urgent task for plant breeders in Russia. The M. G. Safronov Yakut Scientific Research Institute of Agriculture has used wild strawberry forms combining several useful agronomic and biological traits, primarily the resistance to extreme environmental conditions, to achieve this objective. In the efforts to preserve the diversity of crop cultivars, the N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) has developed and is implementing a program to establish nomenclatural standards for domestic cultivars of vegetatively propagated crops. The results of the implementation of this program are presented. Nomenclatural standards for the strawberry cultivars ‘Alexandra’, ‘Pokrovskaya’, ‘Sadovospasskaya’, ‘Vladyka Zosima’ and ‘Bersenevskaya’, released by the M. G. Safronov Yakut Scientific Research Institute of Agriculture, have been formalized, registered in the VIR Herbarium Database, and placed for conservation in the Herbarium of Cultivated Plants of the World, Their Wild Relatives, and Weeds (WIR).

The use of modern breeding methods, biotechnology, and molecular genetics makes it possible to identify promising accessions with specified economically important traits at early pre-breeding stages. The success of creating new varieties depends on the availability of unique collections of plant genetic resources, information about genomes, possibility of in vitro cultivation with high regenerative capacity, and practical skills and competencies in this area. One of the advanced methods for accelerating the breeding process is genome editing using the CRISPR/Cas system. This method allows the effective modification of genes in order to obtain varieties with desired traits. In 2022, a new youth laboratory of genetics, breeding, biotechnology of ornamental and berry crops was set up at VIR as part of the National Project "Science and Universities". It is noteworthy that this event coincided with the 135th anniversary of the birth of N.I. Vavilov. The work of the laboratory is aimed at obtaining lines with desired properties for the further breeding process; identifying target genes of economically important traits for obtaining new varieties, lines, and hybrids; as well as creating protocols for the accelerated reproduction of virus-free material of commercially demanded varieties oriented towards import substitution. This review discusses current trends in breeding of ornamental and berry crops: e.g., flower color change in snapdragon and peony; flower aroma improvement in rose; architectonics change in actinidia; and increase of resistance to stress factors in blackberries, strawberries, and grapes.

In this research, digital technologies that will potentially be useful for teachers in an inclusive educational space were considered. The combined classification of digital technologies of inclusive direction, including software and hardware in three main areas, is offered: for training and repetition; to help with learning; and to expand learning opportunities. The authors developed and theoretically substantiated a model for preparing pre-service teachers to use digital technologies in an inclusive educational space. The effectiveness of the developed model was tested through a pedagogical experiment that involved three tests based on criteria. The effectiveness of the authors' model was confirmed by the sign test at the significance level of 0.05.

In situ transcriptomic techniques promise a holistic view of tissue organization and cell-cell interactions. There has been a surge of multiplexed RNA in situ mapping techniques but their application to human tissues has been limited due to their large size, general lower tissue quality and high autofluorescence. Here we report DART-FISH, a padlock probe-based technology capable of profiling hundreds to thousands of genes in centimeter-sized human tissue sections. We introduce an omni-cell type cytoplasmic stain that substantially improves the segmentation of cell bodies. Our enzyme-free isothermal decoding procedure allows us to image 121 genes in large sections from the human neocortex in 20 healthy and pathological cell states, and identified diseased niches enriched in transcriptionally altered epithelial cells and myofibroblasts.

The purpose of this article is to study types of parental attitude towards children as a way to achieve adaptive family relationships. Methods of the research. The following theoretical methods of the research were used to solve the tasks formulated in the article: a categorical method, structural and functional methods, the methods of the analysis, systematization, modeling, generalization. A pilot research was used as the empirical method. The results of the research. Children of parents with a controlling type of upbringing were obedient, suggestible, fearful, not too persistent in achieving their own goals, non-aggressive. With a mixed style of upbringing children are characterized by suggestibility, obedience, emotional sensitivity, non-aggres­siveness, lack of curiosity, originality of thinking and poor imagination. So, we distinguished three types of parental attitudes that are pathogenic for the emo­tional development of the child: 1. Emotional rejection is the indifferent attitude towards the child, it shows the parents’ unwillingness to satisfy the child’s physi­cal and mental needs, a desire to spend more time without him/her. This also includes cruel treatment, when, along with indifference, parents inflict physical injuries on the child. The reasons are financial difficulties, marital conflicts and mental infantilism of the child. 2. Overprotection is often such style of treatment of the child, which covers up an unconscious rejection of him/her. A feeling of guilt, in the connection with this rejection, often is arisen in parents’ psyche whose child is often and seriously ill. 3. Treatment of the child by a double bind type. The child is completely (and emotionally) dependent on the parents. Conclusions. The classification of parental attitudes towards children suf­fering from neuroses, neurosis-like conditions, psychopathies and character accentuationswas proposed: 1. Indulgent hyperprotection: the child is the cen­ter of attention of the family, and the family strives to satisfy his/her needs as fully as it is possible. 2. Dominant hyperprotection: the child is the center of attention of the parents, who spend a lot of time and energy to him/her, while depriving him/her of independence, setting numerous restrictions and prohibi­tions. 3. Emotional rejection: ignoring the child’s needs, often cruelty to him/her. 4. Increased moral responsibility is in education, increased moral demands are combined with inattention and lack of care on the part of the parents. 5. Cruel treatment includes parents do not strive to satisfy the needs of the child, but at the same time practice cruel punishments for the slightest offenses. 6. Neglect: the child is left to his/her own devices, the parents are not interested in his/her personality and do not control him/her. Lack of care and control are perceived by us as hypoprotection.

The purpose of this article is to show psychological features of post-trau­matic stress disorder of combatants. Methods of the research. The following theoretical methods of the research were used to solve the tasks formulated in the article: a categorical method, structural and functional methods, the methods of the analysis, systematization, modeling and generalization. The experimental method was the method of or­ganizing empirical research. The results of the research. The following methods were shown to be widely used by psychologists and rehabilitation specialists of military units and unit commanders in the practice of psychological rehabilitation of combatants who had received combat mental trauma: autogenic techniques (performance of mental self-regulation techniques, self-training, meditation, etc.); physiologi­cal methods (deep sleep, rest, quality food, drinking a lot of water, shower, field bath); organizational means (establishing a clear regime of work and rest, in­volvement of servicemen into active combat training, service, preservation of military uniform); medication means (sedatives, tranquilizers, antidepressants, nootropics, psychostimulants, phytotherapy, vitamin therapy, etc.); psychotherapeutic techniques and methods (individual and group rational psychotherapy, music, library, art, nature therapy, etc.). Conclusions. We proved that the situation of hostilities, which take place in the east of Ukraine, leads to pronounced changes in the functional state of mental activity, which is characterized by the development of extremely strong negative emotions, such as anxiety, fear, severe mental and physical fatigue. The rapid transition to a peaceful reality often does not allow combatants to adequately respond to certain circumstances from the perspective of the sur­rounding reality. Post-traumatic stress disorder, becoming chronic, affects al­most every aspect of a combatant’s life, including work, activity, interpersonal relationships, physical health and self-esteem.

The paper presents a study of dialect speakers’ lexicon excerpt correlated with the sphere of traditional spiritual culture. A classification of an extensive array of facts recorded whilst surveying the informants in the points of mixed residence in Volgograd region is proposed. The research is carried out in line with the established methodological principles of linguistic units interpretation, and is based on the material extracted from the speech recordings of native speakers of the Don and Ukrainian dialects. The study focused on the key concepts in the spiritual culture of the Russian-Ukrainian population of the region. The concepts are verbalized in the speech of dialect speakers, whose way of life, traditions and customs reflect the life of a nationally heterogeneous continuum. The lexical units relevant for the transition of culturally significant information are defined. It is revealed regarding the content-and-semantic principle of detailing information in the text (within the framework of themes and microthemes) and the semantic-andcategorical means of representing ethnocultural meanings in context (within situations and micro-situations). For residents of mixed settlements, the topics “Ethnic identification of dialect speakers”, “Kinship. Family. Kinship and family relations, traditions”, “Calendar and folk rituals, customs and beliefs”, “Leisure amusements, folk games, fun”, “Nature in traditional spiritual culture”, “Folk medicine, healing”, “Folk etiquette, moral norms of behavior in society, community” are noted to be significant. The article describes the procedure for segmenting an excerpt of dialect speakers’ lexicon into individual topics that reflect traditional culture in its spiritual component; it shows the lexical content of microthemes recorded in the stories of Russian- and Ukrainian-speaking informants; and provides the description of lexical means significant for the realization of these ethno-cultural meanings.

The results of using SLA‑technology in the manufacture of molds for obtaining wax models in lost wax casting are presented. 3D printing modes of molds have been developed to ensure the required roughness of the working surfaces and durability of the resulting molds. The optimal parameters for pressing the wax composition into molds made using SLA technology have been established. It is shown that the use of SLA technology for the manufacture of molds makes it possible to obtain wax models and castings of the required quality. An experimental technology has been developed for the production of castings from bronze by casting according to smelted models.

Enteropathogenic escherichiosis (ЕРЕС) remains to date one of the common causes of the development of gastrointestinal pathology in young children and, depending on the serovar, ЕРЕС can be severe with the development of urgent conditions threatening the patient's life.The purpose of the study: to establish the clinical features of enteropathogenic escherichiosis (ЕРЕС) in young children, depending on the severity of the disease.Materials and methods: the analysis of clinical and laboratory data of 105 cases of ЕРЕС in young children treated at the Regional Infectious Diseases Clinical Hospital named after A.M. Nichoga in Astrakhan during 2013—2023 was carried out.The results: According to the results of our study, it was found that the disease was more often caused by serovars ЕРЕС O111 (59%), O55 (32,4%). Mild and moderate forms of ЕРЕС were 74,3%. Severe forms of HERES (25,7%) are characterized by: acute onset, prolonged (7,9 ± 0,4 days) febrile fever (66,7%); repeated vomiting (91,3%), diarrhea for 9,9 ± 1,8 days with a stool frequency of more than 10 times a day (59.3%) of an abundant, watery nature (96.3%), dehydration of II—III degree (88,9%), the presence of intestinal dysbiosis (96%). In 45,7% of children, ЕРЕС occurred in a moderate form, characterized by subfebrile fever (93.7%) for 5.8 ± 0,9 days, vomiting for 2—3 days (85,4%), watery diarrhea up to 10 times a day (68,7%) lasting 5,9 ± 0,8 days, dehydration of I—II degree (85,4%), intestinal dysbiosis (52%). The difference between mild forms of ЕРЕС (28,6%) was the absence of fever (70%), vomiting (60%), dehydration (100%), and the presence of intestinal dysbiosis in only 16% of children. The nature of the fever, the frequency of vomiting, at the beginning of the disease and subsequently, allows us to judge the severity of the disease, its possible duration, and the effectiveness of treatment. An increase in body temperature to febrile figures, on the 2rd—4th day of the disease, indicates the development of a severe form of ЕРЕС.

Understanding kidney disease relies on defining the complexity of cell types and states, their associated molecular profiles and interactions within tissue neighbourhoods1. Here we applied multiple single-cell and single-nucleus assays (>400,000 nuclei or cells) and spatial imaging technologies to a broad spectrum of healthy reference kidneys (45 donors) and diseased kidneys (48 patients). This has provided a high-resolution cellular atlas of 51 main cell types, which include rare and previously undescribed cell populations. The multi-omic approach provides detailed transcriptomic profiles, regulatory factors and spatial localizations spanning the entire kidney. We also define 28 cellular states across nephron segments and interstitium that were altered in kidney injury, encompassing cycling, adaptive (successful or maladaptive repair), transitioning and degenerative states. Molecular signatures permitted the localization of these states within injury neighbourhoods using spatial transcriptomics, while large-scale 3D imaging analysis (around 1.2 million neighbourhoods) provided corresponding linkages to active immune responses. These analyses defined biological pathways that are relevant to injury time-course and niches, including signatures underlying epithelial repair that predicted maladaptive states associated with a decline in kidney function. This integrated multimodal spatial cell atlas of healthy and diseased human kidneys represents a comprehensive benchmark of cellular states, neighbourhoods, outcome-associated signatures and publicly available interactive visualizations.

У статті зроблено спробу визначити систему кримінальних правопорушень, що пов’язані з перетинанням державного кордону України незаконним способом та / або зі злочинною метою. Доведено, що кримінальні правопорушення, передбачені статтями 332, 332-1, 332-2, 333, 334 розділу XIV Особливої частини Кримінального кодексу України, посягають не просто на недоторканність державних кордонів України, а на прикордонну безпеку, яка є складовою національної безпеки України. Встановлено, в чому полягає відмінність між перетинанням державного кордону України, переміщенням товарів через державний кордон України та переправленням осіб через державний кордон України. На підставі такого порівняння визначено, що перетинання державного кордону України незаконним способом та/або зі злочинною метою характеризується фізичним переміщенням особи через державний кордон України без мети переміщення певного товару чи іншого предмету. Окрім того, на відміну від незаконного переправлення осіб через державний кордон України, перетинанню цього кордону в незаконний спосіб та/або зі злочинною метою властиве безпосереднє фізичне переміщення особи через державний кордон України, яке саме по собі є кримінально караним, якщо здійснюється в незаконний спосіб та/або зі злочинною метою, тоді як незаконне переправлення через державний кордон України характеризується такою властивістю, як опосередкованість, що має першорядне значення для кримінально-правового забезпечення прикордонної безпеки України. До системи кримінальних правопорушень, пов’язаних з перетинанням державного кордону України незаконним способом та/або зі злочинною метою, слід відносити фінансування тероризму у формі надання, збору чи зберігання будь-яких активів прямо чи опосередковано з метою їх використання або усвідомленням можливості того, що їх буде використано повністю або частково для будь-яких цілей окремим терористом чи терористичною групою (організацією) для перетинання державного кордону України з терористичною метою (ст. 258-5), перетинання державного кордону України з терористичною метою (для здійснення діяльності, яка відповідно до закону є терористичною) (ст. 258-6), незаконне перетинання державного кордону України (ст. 332-2), порушення правил міжнародних польотів (ст. 334) Кримінального кодексу України.

The management of heart failure with preserved ejection fraction (HFpEF) remains largely unresolved due to our limited understanding of the underlying mechanisms of the pathology and the challenges in accurately phenotyping this heterogeneous syndrome. A paradigm shift is required to personalise care and unify our approaches to HFpEF patients for future clinical trials. Accordingly, we propose a staging classification for HFpEF based on the identification of the involved cardiac chambers by echocardiography.