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This study aimed to evaluate the effects of 30% hydrogen peroxide (HP) solution containing various contents of 45S5 bioactive glass (BAG) on whitening efficacy and enamel surface properties after simulating the clinical bleaching procedure.A total of 60 bovine enamel specimens discolored with black tea were divided into five groups treated with distilled water (DW), HP, 0.01 wt.% BAG + HP, 1.0 wt.% BAG + HP, and 20.0 wt.% BAG + HP (n = 12). The pH change was observed for 20 min immediately after mixing the experimental solutions, which were applied for 20 min/week, at 37 °C over 21 days. Color, gloss, roughness, microhardness, and micromorphology measurements were conducted before and after bleaching treatment.All groups containing BAG experienced an increase in pH from 3.5 to 5.5 in less than 1 min, and the final pH increased as the BAG content increased. The ΔE of all experimental groups was significantly higher than that of the DW group (p0.05), but there were no significant differences between different BAG contents (p0.05). Gloss significantly decreased in all experimental groups compared to the DW group, and the increased BAG content had significantly affected the decrease in gloss (p0.05). There was no statistical difference in surface roughness (p0.05), but hardness increased significantly with BAG content after bleaching treatment (p0.05).HP containing 45S5 BAG showed efficacy in tooth whitening. Also, the pH value of the HP remained acidic near 3.5 for 20 min, while the HP containing the 45S5 BAG showed an increase in pH, which inhibited the demineralization of the enamel surface, and maintained the surface morphology.These novel materials are promising candidates to minimize enamel surface damage caused by HP during bleaching procedure in dental clinic.

Background. The gaps at the margins of restorative composite resin can increase as the carious process occurs underneath the materials, causing further demineralization along the tooth cavity wall. The aim of this study was to evaluate the effects of restorative resin composite containing hydrated calcium silicate (hCS) filler on enamel protection against demineralization by simulating microleakage between the test material and teeth in a cariogenic environment. Methods. The experimental resin composites were composed of 70 wt% filler, which was mixed with a glass filler and hCS in a weight ratio of 70.0% glass (hCS 0), 17.5% hCS + 52.5% glass (hCS 17.5), 35.0% hCS + 35.0% glass (hCS 35.0), and 52.5% hCS + 17.5% glass (hCS 52.5). A light-cured experimental resin composite disk was positioned over a polished bovine enamel disk, separated by a 30-µm gap, and immersed in artificial saliva with pH 4.0 for 15, 30, and 60 days. After the immersion period, the enamel disk was separated from the resin composite disk and evaluated using a microhardness tester, atomic force microscopy, and polarized light microscopy. The opposing sides of the enamel and resin composite disks were observed using scanning electron microscopy/energy dispersive X-ray spectrometry (SEM/EDS). Results. The enamel surface showed a significant increase in microhardness, decrease in roughness, and remineralization layer as the proportion of hCS increased (P < 0.05). In the SEM image, enamel surface with hCS 35.0 and 52.5 after all experimental immersion periods showed a pattern similar to that of a sound tooth. Conclusions. The results demonstrated that increasing the hCS filler level significantly increased the prevention effect of enamel demineralization compared to restorative resin composites containing conventional dental glass filler. Despite some microleakage exposed to a cariogenic environment, these novel composites may be promising dental biomaterials for protecting teeth against demineralization and preventing secondary caries around the restorations.

The objective of this study was to evaluate an orthodontic adhesive containing hydrated calcium silicate (hCS) in terms of its bond strength with the enamel surface and its acid-neutralization and apatite-forming abilities.The experimental orthodontic adhesives were composed of 30 wt.% resin matrix and 70 wt.% filler, which itself was a mixture of silanized glass filler and hCS in weight ratios of 100% glass filler (hCS 0), 17.5% hCS (hCS 17.5), 35% hCS (hCS 35.0), and 52.5% hCS (hCS 52.5). The degree of conversion (DC) and shear bond strength (SBS) of bovine enamel surfaces were tested. pH measurements were performed immediately upon submersion of the specimens in a lactic acid solution. The surface precipitates that formed on specimens immersed in phosphate-buffered saline (PBS) were analyzed by scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS) and Raman spectroscopy after 15, 30, and 90 days.The experimental groups exhibited no significant differences in DC and had clinically acceptable SBS values. The hCS-containing groups showed increasing pH values as more hCS was added. hCS 52.5 produced Ca- and P-containing surface precipitates after PBS immersion, and hydroxyapatite deposition was detected after 15, 30, and 90 days.These results suggest that orthodontic adhesives containing hCS are effective for acid neutralization. Furthermore, hCS has an apatite-forming ability for enamel remineralization.The novel orthodontic adhesive containing hCS exhibits a potential clinical benefit against demineralization and enhanced remineralization of the enamel surface around or beneath the orthodontic brackets.

Adult bone homeostasis requires a fine-tuned balance between the activity of osteoblasts and osteoclasts. This osteoblast-osteoclast coupling is therapeutically important because it limits the efficacy of most anabolic or anti-resorptive treatments for osteoporosis. Sirtuin6 (SIRT6), a histone deacetylase, was implicated recently as an important regulator in bone homeostasis, but its in vivo function in osteoblast lineage cells remains unclear, mainly due to a lack of in vivo experiments with osteoblast lineage-specific Sirt6 knockout mice. Here, we show that Sirt6 in mature osteoblasts and/or osteocytes inhibits osteoclastogenesis via a paracrine mechanism. We found that osteoblast/osteocyte-specific Sirt6 knockout mice show reduced bone mass due to increased osteoclast formation. Mechanistically, we attribute this increased osteoclastogenesis to decreased osteoprotegerin expression in Sirt6-null osteoblasts and osteocytes. This loss of Sirt6 in osteoblasts and osteocytes does not, however, alter bone formation parameters in vivo. It does accelerate osteogenic differentiation in ex vivo culture, indicating that the osteoblast/osteocyte-autonomous functions of SIRT6 have minor effects on the osteopenic phenotype. These results establish a critical role for SIRT6 in mature osteoblasts and osteocytes in adult bone homeostasis as a negative paracrine regulator of osteoclastogenesis.

Mechanical properties of micro-electro-mechanical systems (MEMS) materials are increasingly important with the wide use of miniaturized systems. This paper deals with development of the strain measurement module for micro materials tests. Vision strain method (VSM) and actuating stroke method (ASM) are adopted in the strain measurement module in order to reduce the discrepancies between the measured deformation and actual one in the specimen. With the developed strain measurement system, a number of tensile tests were concluded for 99.9% nickel thin films with the thickness of 500µm. Specimens with various widths from 1 mm to 2mm and with various lengths from 2mm to 4mm were used in the tests. From the experiment, material properties such as yield strength, tensile strength, fracture strength, fracture strain and young’s modulus are analyzed with stress-strain curves of 99.9% nickel thin film.