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Abstract Vitality Forms (VFs) constitute the dynamic essence of human actions, providing insights into how individuals engage in activities. The ability to perceive and express VFs during interpersonal interactions is pivotal for understanding others’ intentions, behaviors, and fostering effective social communication. Despite their ubiquity in all actions, research exploring the role of VFs in neurodivergent conditions related to social and communicative skills, particularly in autism, remains limited. This study aims to investigate the expression of different VFs during the execution of both social and non-social actions in children with an Autism Spectrum Condition (ASC) in comparison to neurotypical children (NT). ASC children and NT children were asked to move a small bottle either towards a target point (non-social context) or moving it towards a receiver (social context) with different VFs specifically neutral, gentle, or rude. Videotaped tasks were subsequently analyzed to study kinematic parameters characterizing VFs. Our results highlighted three main findings: (1) overall, ASC children are able to tune the motor profile of their actions, effectively conveying both gentle and rude VFs; (2) distinct kinematic parameters in the execution of VFs are able to distinguish autistic children from NT children; (3) the social context significantly influences the child’s ability to express positive and negative VFs in autism. Taken together, these findings provide new insights to understand how VFs contribute to the complex dynamics of social communication in neurodivergent autistic children, providing a valuable contribution for future interventions and support strategies.

We present the first spectroscopic estimates of the chemical abundance of M dwarf stars in a globular cluster (GC), namely 47 Tucanae. By exploiting NIRSpec on board the James Webb Space Telescope, we gathered low-resolution spectra for 28 stars with masses in the range ∼0.4–0.5 M _⊙ . The spectra are strongly affected by the H _2 O water vapor bands, which can be used as indicators of oxygen abundance. The spectral analysis reveals that the target stars feature a different O abundance, with a difference of ∼0.40 dex between the first and the most polluted second population. The observed range is similar to that observed among red giant stars. This result reinforces previous findings based on the analysis of photometric diagrams, including the “chromosome maps,” providing a first, and more direct, evidence of light element variations in the M dwarfs’ mass regime. The observation that the multiple populations, with their variations in light elements, exhibit the same patterns from the lower main sequence all the way to the red giant branch further strengthens the notion that multiple stellar populations in GCs formed in a series of bursts of star formation.

An overview is presented of the progress since 2021 in the construction and scientific programme preparation of the Divertor Tokamak Test (DTT) facility. Licensing for building construction has been granted at the end of 2021. Licensing for Cat. A radiologic source has been also granted in 2022. The construction of the toroidal field magnet system is progressing. The prototype of the 170 GHz gyrotron has been produced and it is now under test on the FALCON facility. The design of the vacuum vessel, the poloidal field coils and the civil infrastructures has been completed. The shape of the first DTT divertor has been agreed with EUROfusion to test different plasma and exhaust scenarios: single null, double null, X-divertor and negative triangularity plasmas. A detailed research plan is being elaborated with the involvement of the EUROfusion laboratories.

James Webb Space Telescope (JWST) observations have been demonstrated to be efficient in detecting multiple stellar populations in globular clusters (GCs) in the low-mass regime of M dwarfs. We present an overview, and first results, of different projects that can be explored by using the JWST observations gathered under program GO2560 for 47 Tucanae, the first program entirely devoted to the investigation of multiple populations in very-low-mass stars, which includes spectroscopic data for the faintest GC stars for which spectra are available. Our color–magnitude diagram (CMD) shows some substructures for ultracool stars, including gaps and breaks in slope. In particular, we observe both a gap and a minimum in the F322W2 luminosity function less than 1 mag apart, and discuss which it could be associated with the H-burning limit. We detect stars fainter than this minimum, very likely brown dwarfs. We corroborate the ubiquity of the multiple populations across different masses, from ∼0.1 M _⊙ up to red giants (∼0.8 M _⊙ ). The oxygen range inferred for the M dwarfs, both from the CMD and from the spectra of two M dwarfs associated with different populations, is similar to that observed for giants. We have not detected any difference between the fractions of stars in distinct populations across stellar masses ≳ 0.1 M _⊙ . This work demonstrates the JWST's capability in uncovering multiple populations within M dwarfs and illustrates the possibility to analyze very-low-mass stars in GCs approaching the H-burning limit and the brown-dwarf sequence.

The nature of young star clusters in the Magellanic Clouds is debated. Here, the authors show an alternative approach that exploits data to exclude the presence of age differences greater than a few million years among cluster stars in a very young cluster.

Abstract Cardiac complications in patients with COVID-19 have been described in the literature with an important impact on outcome. The primary objective of our systematic review was to describe the kind of cardiac complications observed in COVID-19 patients and to identify potential predictors of cardiovascular events. The secondary aim was to analyze the effect of cardiac complications on outcome. We performed this systematic review according to PRISMA guidelines using several databases for studies evaluating the type of cardiac complications and risk factors in COVID-19 patients. We also calculated the risk ratio (RR) and 95% CI. A random-effects model was applied to analyze the data. The heterogeneity of the retrieved trials was evaluated through the I 2 statistic. Our systematic review included 49 studies. Acute cardiac injury was evaluated in 20 articles. Heart failure and cardiogenic shock were reported in 10 articles. Myocardial infarction was evaluated in seven of the papers retrieved. Takotsubo, myocarditis, and pericardial effusion were reported in six, twelve, and five articles, respectively. Arrhythmic complications were evaluated in thirteen studies. Right ventricular dysfunction was evaluated in six articles. We included 7 studies investigating 2115 patients in the meta-analysis. The RR was 0.20 (95% CI: 0.17 to 0.24; P < 0.00001, I 2 = 0.75). Acute cardiac injury represented the prevalent cardiac complications observed in COVID-19 patients (from 20 to 45% of the patients). Patients with acute cardiac injury seemed to be significantly older, with comorbidities, more likely to develop complications, and with higher mortality rates. Acute cardiac injury was found to be an independent risk factor for severe forms of SARS-CoV-2 infection and an independent predictor of mortality. Due to the scarce evidence, it was not possible to draw any conclusion regarding Takotsubo, myocarditis, pleural effusion, and right ventricular dysfunction in COVID-19 patients. Noteworthy, possible arrhythmic alterations (incidence rate of arrhythmia from 3 to 60%) in COVID-19 patients have to be taken into account for the possible complications and the consequent hemodynamic instabilities. Hypertension seemed to represent the most common comorbidities in COVID-19 patients (from 30 to 59.8%). The prevalence of cardiovascular disease (CVD) was high in this group of patients (up to 57%), with coronary artery disease in around 10% of the cases. In the majority of the studies retrieved, patients with CVD had a higher prevalence of severe form, ICU admission, and higher mortality rates.

Abstract The data produced by the scientific community impacts on academia, clinicians, and the general public; therefore, the scientific community and other regulatory bodies have been focussing on ethical codes of conduct. Despite the measures taken by several research councils, unethical research, publishing and/or reviewing behaviours still take place. This exploratory study considers some of the current unethical practices and the reasons behind them and explores the ways to discourage these within research and other professional disciplinary bodies. These interviews/discussions with PhD students, technicians, and academics/principal investigators (PIs) (N=110) were conducted mostly in European higher education institutions including UK, Italy, Ireland, Portugal, Czech Republic and Netherlands. Through collegiate discussions, sharing experiences and by examining previously published/reported information, authors have identified several less reported behaviours. Some of these practices are mainly influenced either by the undue institutional expectations of research esteem or by changes in the journal review process. These malpractices can be divided in two categories relating to (a) methodological malpractices including data management, and (b) those that contravene publishing ethics. The former is mostly related to “committed bias”, by which the author selectively uses the data to suit their own hypothesis, methodological malpractice relates to selection of out-dated protocols that are not suited to the intended work. Although these are usually unintentional, incidences of intentional manipulations have been reported to authors of this study. For example, carrying out investigations without positive (or negative) controls; but including these from a previous study. Other methodological malpractices include unfair repetitions to gain statistical significance, or retrospective ethical approvals. In contrast, the publication related malpractices such as authorship malpractices, ethical clearance irregularities have also been reported. The findings also suggest a globalised approach with clear punitive measures for offenders is needed to tackle this problem.

Abstract Non-aureus staphylococci are commonly found on dairy farms. Two rarely investigated species are Staphylococcus (S.) cohnii and S. urealyticus. Since multidrug-resistant S. cohnii and S. urealyticus are known, they may serve as an antimicrobial resistance (AMR) gene reservoir for harmful staphylococcal species. In our study, nine S. cohnii and six S. urealyticus isolates from German dairy farms were analyzed by whole-genome sequencing and AMR testing. The isolates harbored various AMR genes (aadD1, str, mecA, dfrC/K, tetK/L, ermC, lnuA, fexA, fusF, fosB6, qacG/H) and exhibited non-wildtype phenotypes (resistances) against chloramphenicol, clindamycin, erythromycin, fusidic acid, rifampicin, streptomycin, tetracycline, tiamulin and trimethoprim. Although 14/15 isolates lacked the blaZ, mecA and mecC genes, they showed reduced susceptibility to a number of beta-lactam antibiotics including cefoxitin (MIC 4–8 mg/L) and penicillin (MIC 0.25–0.5 mg/L). The specificity of cefoxitin susceptibility testing for mecA or mecC gene prediction in S. cohnii and S. urealyticus seems to be low. A comparison with penicillin-binding protein (PBP) amino acid sequences of S. aureus showed identities of only 70–80% with regard to PBP1, PBP2 and PBP3. In conclusion, S. cohnii and S. urealyticus from selected German dairy farms show multiple resistances to antimicrobial substances and may carry unknown antimicrobial resistance determinants.

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The “chromosome maps” (ChMs) of globular clusters (GCs) have revealed that these ancient structures are inhomogeneous in metallicity in various ways and in different natures. Type II GCs generally display larger variations, sometimes coupled with slow neutron-capture ( s ) element enrichments on the ChMs redder sequences, which have been interpreted as due to multiple generations of stars. On the other hand, most GCs have inhomogeneous first populations (1P) in the form of large ranges in the Δ _F275W,F814W values, pointing toward a not fully mixed pristine molecular cloud. We analyze the chemical composition of GC 47 Tucanae, which shows both inhomogeneous 1P stars and, although not formally a Type II GC, hosts a small number of stars distributed on the red side of the main stream of ChM stars. Our results suggest that 1P stars are inhomogeneous in the overall metallicity, with variations on the order of ∼0.10 dex in all the chemical species. The anomalous stars distributed on a redder sequence of the ChM are further enriched in metals but have no evidence for a significant enrichment in the s elements. Our three second population stars located on the normal component of the map have metallicities similar to those of the metal-richer 1P oup, suggesting that this population formed from these stars. Although three stars is a too-small sample to draw strong conclusions, the low spread in metals of these objects might point toward formation in a fully mixed medium, possibly after a cooling flow phase.

Recent work on metal-intermediate globular clusters (GCs) with [Fe/H] = −1.5 and −0.75 has illustrated the theoretical behavior of multiple populations in photometric diagrams obtained with the JWST. These results are confirmed by observations of multiple populations among the M dwarfs of 47 Tucanae. Here we explore multiple populations in metal-poor GCs with [Fe/H] = −2.3. We take advantage of synthetic spectra and isochrones that account for the chemical composition of multiple populations to identify photometric diagrams that separate the distinct stellar populations of GCs. We derive high-precision photometry and proper motion for main-sequence (MS) stars in the metal-poor GC M92 from JWST and Hubble Space Telescope images. We identify a first-generation (1G) and two main groups of second-generation (2G _A and 2G _B ) stars and investigate their kinematics and chemical composition. We find isotropic motions with no differences among the distinct populations. The comparison between the observed colors of the M92 stars and the colors derived by synthetic spectra reveals that the helium abundances of 2G _A and 2G _B stars are higher than those of the 1G by Δ Y ∼ 0.01 and 0.04, respectively. The m _F090W versus m _F090W − m _F277W color–magnitude diagram shows that below the knee MS stars exhibit a wide color broadening due to multiple populations. We constrain the amount of oxygen variation needed to reproduce the observed MS width, which is consistent with results on red giant branch stars. We conclude that multiple populations with masses of ∼0.1–0.8 M _⊙ share similar chemical compositions.

We report on the two-dimensional (2D) dynamics of a levitated nanoparticle in an optical cavity. The motion of the nanosphere is strongly coupled to the cavity field by coherent scattering and heavily cooled in the plane orthogonal to the tweezer axis. Due to the characteristics of the 2D motion and the strong optomechanical coupling, the motional sideband asymmetry that reveals the quantum nature of the dynamics is not limited to mere scale factors between Stokes and anti-Stokes peaks, as customary in quantum optomechanics, but assumes a peculiar spectral dependence. We introduce and discuss an effective thermal occupancy that quantifies how close the system is to a minimum uncertainty state and allows us to consistently characterize the particle motion. By rotating the polarization angle of the tweezer beam we tune the system from a 1D cooling regime, where we achieve a best thermal occupancy of 0.51±0.05, to a regime in which the fully 2D dynamics of the particle exhibit strong nonclassical properties. We achieve a strong 2D confinement with thermal occupancy of 3.4±0.4 along the warmest direction and around one in the orthogonal one. These results represent a major improvement over previous experiments considering both the 1D and the 2D motion and pave the way toward the preparation of tripartite optomechanical entangled states and novel applications to directional force and displacement quantum sensing.

We review the multiple population (MP) phenomenon of globular clusters (GCs): i.e., the evidence that GCs typically host groups of stars with different elemental abundances and/or distinct sequences in photometric diagrams. Most Galactic and extragalactic clusters exhibit internal variations of He, C, N, O, Na, and Al. They host two distinct stellar populations: the first population of stars, which resemble field stars with similar metallicities, and one or more second stellar populations that show the signature of high-temperature H-burning. In addition, a sub-sample of clusters hosts stellar populations with different heavy-element abundances. The MP origin remains one of the most puzzling, open issues of stellar astrophysics. We summarize the scenarios for the MP formation and depict the modern picture of GCs and their stellar populations along with the main evolutionary phases. We show that the MP behavior dramatically changes from one cluster to another and investigate their complexity to define common properties. We investigate relations with the host galaxy, the parameters of the host clusters (e.g., GC’s mass, age, orbit), and stellar mass. We summarize results on spatial distribution and internal kinematics of MPs. Finally, we review the relation between MPs and the so-called second-parameter problem of the horizontal-branch morphology of GCs and summarize the main findings on the extended main sequence phenomenon in young clusters.

Spillover of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) to North American white-tailed deer (Odocoileus virginianus) has been documented. However, it is unclear if this is a phenomenon specific to North American deer or is a broader problem. We evaluated pre and pandemic exposure of German and Austrian deer species using a SARS-CoV-2 pseudoneutralization assay. In stark contrast to North American white-tailed deer, we found no evidence of SARS-CoV-2 exposure.

This article reports a case of a congenital unilateral forelimb defect in a two-month-old male puppy with clinical and radiographic findings. Congenital deformities are structural or functional congenital defects arising from errors during development, and they can affect an isolated portion of the body, the complete body or parts of several systems of the body. The congenital absence of a portion of the proximal epiphysis of the radius is an extremely rare condition in human and animal species. Although similar congenital limb deformities have been infrequently reported in dogs, the present case is, to the best of the authors' knowledge, the first report of this type of partial forelimb paraxial radial hemimelia in a puppy, and it represents an addition to the scant literature on this topic.

This report describes the clinical, parasitological and pathological findings in a 6-year-old intact female European cat with thoracic and peritoneal tetrathyridiosis, characterized by genital involvement. Physical examination and X-ray evaluation revealed laboured breathing and several pulmonary nodules suggestive of cancer. However, necropsy demonstrated a parasitic aetiology of the disease. Histologically, multifocal granulomas were detected in the lungs, uterus and ovary. Parasitological examination permitted identification of the intestinal parasites as Mesocestoides lineatus, which was later confirmed by molecular examination. The larval forms in the peritoneal and chest cavity were identified as the second stage of the Mesocestoides sp. cestode named Tetrathyridia spp. The chronic injuries observed and the rapid course of the disease from the onset of the symptoms until death suggested a long period without clinical signs and indicate that overt disease can potentially be triggered by a failure of the immune system. The observed oophoritis and metritis identify tetrathyridiosis as a possible differential diagnosis in genital dysfunction.

Summary: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is emerging as a public health problem worldwide. In Italy, a remarkable increase in CRKP cases has been reported since 2010. In this study, CRKP diffusion, distribution and in-hospital transmission trends were evaluated in a university hospital in Milan, Italy, from January 2012 to December 2013. Isolates from 63 newly detected CRKP-positive patients were genotyped, and possible transmission was determined by combining the molecular results with data concerning the patients’ admission and in-hospital transfers. Most of the cases (90.4%) were from general medical and surgery wards, and the remaining 9.6% were from the intensive care unit. Fifteen of the 46 hospital-associated cases (32.6%) were attributable to in-hospital transmission. After the introduction of targeted and hospital-wide control measures, the transmission index significantly decreased from 0.65 to 0.13 (p = 0.01). There was also a decrease in the overall nosocomial case incidence, from 0.37 to 0.17 per 1000 person-days (p = 0.07).Our findings indicate that the spread of CRKP in Northern Italy hospitals may go far beyond high-risk settings (i.e., intensive care units) and that strict surveillance should be extended to general areas of care. Keywords: Multidrug-resistant agents, Carbapenem-resistant Klebsiella pneumoniae, Cross-transmission, Infections control measures, Active surveillance, Active screening

The tomato (Solanum lycopersicum L.) is one of the many essential vegetables around the world due to its nutritive content and attractive flavor. However, its short shelf-life and postharvest losses affect its marketing. In this study, the effects of chitosan-Ruta graveolens (CS + RGEO) essential oil coatings on the postharvest quality of Tomato var. “chonto” stored at low temperature (4 °C) for 12 days are reported. The film-forming dispersions (FFD) were eco-friendly synthesized and presented low viscosities (between 0.126 and 0.029 Pa s), small particle sizes (between 1.29 and 1.56 μm), and low densities. The mature index (12.65% for uncoated fruits and 10.21% for F4 coated tomatoes), weight loss (29.8% for F1 and 16.7% for F5 coated tomatoes), and decay index (3.0 for uncoated and 1.0 for F5 coated tomatoes) were significantly different, indicating a preservative effect on the quality of the tomato. Moreover, aerobic mesophilic bacteria were significantly reduced (in five Log CFU/g compared to control) by using 15 μL/mL of RGEO. The coatings, including 10 and 15 μL/mL of RGEO, completely inhibited the mold and yeast growth on tomato surfaces without negatively affecting the consumer acceptation, as the sensorial analysis demonstrated. The results presented in this study show that CS + RGEO coatings are promising in the postharvest treatment of tomato var. “chonto”.

Stimulation of renal collecting duct principal cells with antidiuretic hormone (arginine-vasopressin, AVP) results in inhibition of the small GTPase RhoA and the enrichment of the water channel aquaporin-2 (AQP2) in the plasma membrane. The membrane insertion facilitates water reabsorption from primary urine and fine-tuning of body water homeostasis. Rho guanine nucleotide exchange factors (GEFs) interact with RhoA, catalyze the exchange of GDP for GTP and thereby activate the GTPase. However, GEFs involved in the control of AQP2 in renal principal cells are unknown. The A-kinase anchoring protein, AKAP-Lbc, possesses GEF activity, specifically activates RhoA, and is expressed in primary renal inner medullary collecting duct principal (IMCD) cells. Through screening of 18,431 small molecules and synthesis of a focused library around one of the hits, we identified an inhibitor of the interaction of AKAP-Lbc and RhoA. This molecule, Scaff10-8, bound to RhoA, inhibited the AKAP-Lbc-mediated RhoA activation but did not interfere with RhoA activation through other GEFs or activities of other members of the Rho family of small GTPases, Rac1 and Cdc42. Scaff10-8 promoted the redistribution of AQP2 from intracellular vesicles to the periphery of IMCD cells. Thus, our data demonstrate an involvement of AKAP-Lbc-mediated RhoA activation in the control of AQP2 trafficking.

A rare case of a liver biliary duct cystoadenoma in a dog is reported. Tissue changes are described with an emphasis on immunohistochemical patterns that aid in the identification of the tumour and differentiation from similar macroscopical features that arise due to congenital abnormalities. Finally, we provide hints on comparative pathology.

According to embodied cognition, language processing relies on the same neural structures involved when individuals experience the content of language material. If so, processing nouns expressing a motor content presented in a second language should modulate the motor system as if presented in the mother tongue. We tested this hypothesis using a go-no go paradigm. Stimuli included English nouns and pictures depicting either graspable or non-graspable objects. Pseudo-words and scrambled images served as controls. Italian participants, fluent speakers of English as a second language, had to respond when the stimulus was sensitive and refrain from responding when it was not. As foreseen by embodiment, motor responses were selectively modulated by graspable items (images or nouns) as in a previous experiment where nouns in the same category were presented in the native language.

A case of a unilateral forelimb congenital defect in a three-month-old female puppy dog with clinical and radiographic findings is described here. Congenital absence of humerus with preaxial terminal longitudinal hemimelia is a rare condition in human and animal species. Although similar congenital limb deformities in dogs have previously been documented, the present case is, to the authors' knowledge, the first report of this type of forelimb hemimelia in a dog, and represents an addition to the scant literature on this topic.