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Bakgrund: Den dagliga fysiska aktiviteten hos barn minskar, nya aktiviteter och intressen som utförs framför dator är en bidragande faktor till detta. Ökat stillasittande kan leda till ett flertal konsekvenser. Fysisk aktivitet kan motverka detta och bidrar till förbättrad kondition, muskelstyrka, blodtryck, blodfetter, bentäthet och BMI hos barn och unga i skolåldrarna. Syfte: Att sammanställa hur fysisk aktivitet under skoldagen för elever (åldern 6–19) påverkar skolprestationen. Metod: Denna systematiska litteraturstudie baseras på 13 vetenskapliga artiklar. Studierna har en kvantitativ ansats, och var genomförda med randomiserade kontroll studier RCT). En kvalitetsgranskning av materialet gjordes med hjälp av GRADE. Resultat: I resultatet framkommer inget enhälligt resultat gällande hur fysisk aktivitet påverkar elevers skolprestation. Studierna visar i stället på tre huvudområden som sammanfattas till positivt resultat på skolprestation efter intervention, ingen förändring på skolprestation efter intervention och slutligen andra effekter av intervention. Slutsats: Ingen generell slutsats har kunnat dras gällande ökad fysisk aktivitet och elevers skolprestation. Endast en liten del kan ha påverkats negativ av interventionen ökad fysisk aktivitet under skoldagen. Generellt kan ingen negativ påverkan ses på elevernas skolprestation efter interventionerna. Forskning visar också på att elever som hade fått ta del av dessa insatser utveckla färre problem med ångest, jämfört med barn i en kontrollgrupp., Background: The daily physical activity of children decreases, new activities and interests performed in front of a computer are a contributing factor to this. Increased sedentary lifestyle can lead to a few consequences. Physical activity can counteract this and contribute to improved fitness, muscle strength, blood pressure, blood fats, bone density and BMI in children and adolescent of school age. Aim: To compile how physical activity during the school day for students (age 6-19) affects school performance. Method: This systematic literature study is based on 13 scientific articles. The studies have a quantitative approach and were conducted with randomized control studies (RCT). A quality review of the material was done with the help of GRADE. Result: The result does not show a unanimous result regarding how physical activity affects students' school performance. Instead, the studies show three main areas that are summarized as a positive result on school performance after intervention, no change in school performance after intervention and finally other effects of intervention. Conclusion: No general conclusion has been drawn regarding increased physical activity and students' school performance. Only a small part may have been negatively affected by the increased physical activity during the school day. In general, no negative impact can be seen on students' school performance after the interventions. Research also shows that students who have taken part in these interventions develop fewer problems with anxiety, compared with children in a control group.

Nickel (Ni) compounds are classified as carcinogenic to humans but the underlying mechanisms are still poorly understood. Furthermore, effects related to nanoparticles (NPs) of Ni have not been fully elucidated. The aim of this study was to investigate genotoxicity and mutagenicity of Ni and NiO NPs and compare the effect to soluble Ni from NiCl2. We employed different models; i.e., exposure of (1) human bronchial epithelial cells (HBEC) followed by DNA strand break analysis (comet assay and -H2AX staining); (2) six different mouse embryonic stem (mES) reporter cell lines (ToxTracker) that are constructed to exhibit fluorescence upon the induction of various pathways of relevance for (geno)toxicity and cancer; and (3) mES cells followed by mutagenicity testing (Hprt assay). The results showed increased DNA strand breaks (comet assay) for the NiO NPs and at higher doses also for the Ni NPs whereas no effects were observed for Ni ions/complexes from NiCl2. By employing the reporter cell lines, oxidative stress was observed as the main toxic mechanism and protein unfolding occurred at cytotoxic doses for all three Ni-containing materials. Oxidative stress was also detected in the HBEC cells following NP-exposure. None of these materials induced the reporter related to direct DNA damage and stalled replication forks. A small but statistically significant increase in Hprt mutations was observed for NiO but only at one dose. We conclude that Ni and NiO NPs show more pronounced (geno)toxic effects compared to Ni ions/complexes, indicating more serious health concerns. Environ. Mol. Mutagen. 59:211-222, 2018., QC 20180404

Background: Cell fusion is a natural process in normal development and tissue regeneration. Fusion between cancer cells and macrophages generates metastatic hybrids with genetic and phenotypic characteristics from both maternal cells. However, there are no clinical markers for detecting cell fusion in clinical context. Macrophage-specific antigen CD163 expression in tumor cells is reported in breast and colorectal cancers and proposed being caused by macrophages-cancer cell fusion in tumor stroma. The purpose of this study is to examine the cell fusion process as a biological explanation for macrophage phenotype in breast. Methods: Monocytes, harvested from male blood donor, were activated to M2 macrophages and co-cultured in ThinCert transwell system with GFP-labeled MCF-7 cancer cells. MCF7/macrophage hybrids were generated by spontaneous cell fusion, isolated by fluorescence-activated cell sorting and confirmed by fluorescence microscopy, short tandem repeats analysis and flow cytometry. CD163 expression was evaluated in breast tumor samples material from 127 women by immunohistochemistry. Results: MCF-7/macrophage hybrids were generated spontaneously at average rate of 2 % and showed phenotypic and genetic traits from both maternal cells. CD163 expression in MCF-7 cells could not be induced by paracrine interaction with M2-activated macrophages. CD163 positive cancer cells in tumor sections grew in clonal collection and a cutoff point greater than25 % of positive cancer cells was significantly correlated to disease free and overall survival. Conclusions: In conclusion, macrophage traits in breast cancer might be caused by cell fusion rather than explained by paracrine cellular interaction. These data provide new insights into the role of cell fusion in breast cancer and contributes to the development of clinical markers to identify cell fusion., Funding Agencies|County Council of Ostergotland (Sweden); National Organization of Breast Cancer Associations (Sweden); Bengt Ihre Foundation (Swedish Surgical Society)

Women are subordinate of men in the society, this is visible both in work places and private lives. Equality between the sexes is daily discussed in politics and the private sphere. As this is a hot topic which is debated within many areas, one of those is film production, we chose to write this essay about how the view of women has changed in movies over time. If equality doesn’t exist within the film media, then it will be hard to change the society’s point of view. To get the answer of our question, we chose to analyze three movie pictures from three different time areas, the choice came to be the three King Kong movies that were produced in 1933, 1976 and 2005. After we finished analyzing the films we came to learn that society’s view of women has changed drastically between the 1930’s and the 21st Century. The changing progress has been both positive and negative because of the fact that women in today’s society are exploited more as sex objects than they used to be. But even in the 30’s men looked at women in a sexual matter, but in those days the difference between the respectable women and those time’s pin-up girls. On the other hand, equality between the sexes has come a long way in the work place and women have gained more independence in their private lives. But there is still a long way to go before we reach a complete equality.

3D cell culture models are an important tool in translational research but have been out of reach for high-throughput screening due to complexity, requirement of large cell numbers and inadequate standardization. Here, we present a high-throughput workflow to produce and characterize the formation of miniaturized spheroids using deep learning. We train a convolutional neural network (CNN) for cell ensemble morphology classification, benchmark it against more conventional image analysis, and characterize spheroid assembly determining optimal surfactant concentrations and incubation times for spheroid production for three cell lines with different spheroid formation properties. Notably, this format is compatible with large-scale spheroid production and screening. The presented workflow and CNN offer a template for large scale minispheroid production and analysis and can be extended and re-trained to characterize morphological responses in spheroids to additives, culture conditions and large drug libraries., QC 20221129