1. Dynamics of insecticide resistance in Anopheles gambiae sensu lato populations from Bohicon in Sudano-guinean area of Benin, West Africa
- Author
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Aïzoun, Nazaire, Adjatin, Arlette, Assongba, Faustin, and Géorcelin Alowanou
- Subjects
Dynamics ,Resistance ,Insecticides ,Malaria ,Benin - Abstract
The current study was aimed to investigate the dynamics of insecticide resistance inAnopheles gambiaesensu lato populations from Bohicon in Sudano-guinean area of Benin, West Africa. Larvae and pupae ofAnopheles gambiae s.l.populations were collected from the breeding sites in Zou department in 2015 and 2020. WHO susceptibility tests were conducted on unfed female mosquitoes aged 2-5 days old. WHO bioassays were performed with impregnated papers of permethrin (0.75%) and dichlorodiphenyltrichloroethane (DDT) (4%).An. gambiaemosquitoes were identified to species using PCR techniques in 2015. Molecular assays were also carried out to identifykdrmutations in individual mosquitoes.An. Gambiae s.l.populations from Bohicon were resistant to permethrin and DDT in 2015 and still remained resistant to these products in 2020. There is cross-resistance to both insecticides. PCR revealed 100% of mosquitoes tested wereAnopheles gambiae s.s. TheL1014F kdrmutation was found inAn. Gambiae s.s.Bohicon at high allelic frequency.
- Published
- 2022