1. Neuroblast Migration and P2Y1 Receptor Mediated Calcium Signalling Depend on 9-O-Acetyl GD3 Ganglioside
- Author
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Eliana Scemes and Marcelo F. Santiago
- Subjects
P2Y1 receptor ,MAP, microtubule-associated protein ,Mice ,Receptors, Purinergic P2Y1 ,Adenosine Triphosphate ,0302 clinical medicine ,Neural Stem Cells ,Cell Movement ,Tubulin ,Cerebellum ,Gangliosides ,J-Ab, Jones antibody ,Internalization ,Receptor ,media_common ,Calcium signaling ,Mice, Knockout ,0303 health sciences ,DMEM, Dulbecco's modified Eagle's medium ,General Neuroscience ,Gene Expression Regulation, Developmental ,Cell biology ,Adenosine Diphosphate ,eGFP, enhanced green fluorescent protein ,Jones antibody ,Purinergic P2Y Receptor Agonists ,Microtubule-Associated Proteins ,Research Article ,media_common.quotation_subject ,Green Fluorescent Proteins ,Immunocytochemistry ,chemistry.chemical_element ,S9 ,cerebellar granule cell ,Biology ,Calcium ,Transfection ,Antibodies ,03 medical and health sciences ,Organ Culture Techniques ,In vivo ,Neuroblast migration ,Glial Fibrillary Acidic Protein ,Animals ,9acGD3, 9-O-acetyl GD3 ,Calcium Signaling ,030304 developmental biology ,DPBS, Dulbecco's PBS ,Ganglioside ,calcium signalling and neuronal migration ,GFAP, glial fibrillary acidic protein ,GD3 synthase ,WT, wild-type ,Molecular biology ,GCP, granule cell progenitor ,Animals, Newborn ,chemistry ,Purinergic P2Y Receptor Antagonists ,Neurology (clinical) ,P2R, purinergic P2 receptor ,030217 neurology & neurosurgery ,DAPI, 4′,6-diamidino-2-phenylindole - Abstract
Previous studies indicated that a ganglioside 9acGD3 (9-O-acetyl GD3) antibody [the J-Ab (Jones antibody)] reduces GCP (granule cell progenitor) migration in vitro and in vivo. We here investigated, using cerebellar explants of postnatal day (P) 6 mice, the mechanism by which 9acGD3 reduces GCP migration. We found that immunoblockade of the ganglioside with the J-Ab or the lack of GD3 synthase reduced GCP in vitro migration and the frequency of Ca2+ oscillations. Immunocytochemistry and pharmacological assays indicated that GCPs expressed P2Y1Rs (P2Y1 receptors) and that deletion or blockade of these receptors decreased the migration rate of GCPs and the frequency of Ca2+ oscillations. The reduction in P2Y1-mediated calcium signals seen in Jones-treated and GD3 synthase-null GCPs were paralleled by P2Y1R internalization. We conclude that 9acGD3 controls GCP migration by influencing P2Y1R cellular distribution and function.
- Published
- 2012
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