1. The double-stranded DNA-binding proteins TEBP-1 and TEBP-2 form a telomeric complex with POT-1
- Author
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Christian Renz, Miguel Vasconcelos Almeida, Emily Nischwitz, Jan Schreier, Albert Fradera-Sola, Nikenza Viceconte, Dennis Kappei, Helle D. Ulrich, Alejandro Ceron-Noriega, Falk Butter, René F. Ketting, Sabrina Dietz, Dietz, Sabrina [0000-0003-3227-9264], Almeida, Miguel Vasconcelos [0000-0002-4816-3111], Viceconte, Nikenza [0000-0002-5101-4125], Fradera-Sola, Albert [0000-0002-4780-9312], Kappei, Dennis [0000-0002-3582-2253], Ketting, René F [0000-0001-6161-5621], Butter, Falk [0000-0002-7197-7279], Apollo - University of Cambridge Repository, and Ketting, René F. [0000-0001-6161-5621]
- Subjects
DNA damage ,Science ,Mutant ,Telomere-Binding Proteins ,Biology ,631/45/475 ,Double-stranded DNA binding ,Germline ,38/70 ,570 Life sciences ,Animals, Genetically Modified ,82/80 ,14/32 ,38/23 ,64/11 ,Animals ,Protein Isoforms ,14/19 ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Mitosis ,82/83 ,Phylogeny ,Binding Sites ,Base Sequence ,82/58 ,article ,DNA ,631/80/103/560 ,Telomere ,Shelterin ,Phenotype ,Cell biology ,DNA-Binding Proteins ,Germ Cells ,Microscopy, Fluorescence ,Multiprotein Complexes ,38/35 ,Mutation ,14/63 ,570 Biowissenschaften ,Protein Binding ,82/111 - Abstract
Telomeres are bound by dedicated protein complexes, like shelterin in mammals, which protect telomeres from DNA damage. In the nematodeCaenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approach to screen for proteins binding toC. elegans telomeres, and identified TEBP-1 and TEBP-2, two paralogs that associate to telomeres in vitro and in vivo. TEBP-1 and TEBP-2 are expressed in the germline and during embryogenesis.tebp-1andtebp-2mutants display strikingly distinct phenotypes:tebp-1mutants have longer telomeres than wild-type animals, whiletebp-2mutants display shorter telomeres and a mortal germline, a phenotype characterized by transgenerational germline deterioration. Notably,tebp-1;tebp-2double mutant animals have synthetic sterility, with germlines showing signs of severe mitotic and meiotic arrest. TEBP-1 and TEBP-2 form a telomeric complex with the known single-stranded telomere-binding proteins POT-1, POT-2, and MRT-1. Furthermore, we find that POT-1 bridges the double- stranded binders TEBP-1 and TEBP-2, with the single-stranded binders POT-2 and MRT-1. These results describe the first telomere-binding complex inC. elegans, with TEBP-1 and TEBP-2, two double-stranded telomere binders required for fertility and that mediate opposite telomere dynamics.
- Published
- 2021
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