1. Selective blockade of phosphodiesterase types 2, 5 and 9 results in cyclic 3′5′ guanosine monophosphate accumulation in retinal pigment epithelium cells
- Author
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E. C. La Heij, Fred Hendrikse, Roselie M.H. Diederen, M. Markerink-van Ittersum, Aize Kijlstra, J. de Vente, and Ophthalmology
- Subjects
RNA, Messenger/genetics ,Exonucleases ,Male ,Phosphodiesterase Inhibitors ,Gene Expression ,Exonucleases/antagonists & inhibitors ,chemistry.chemical_compound ,3',5'-Cyclic-AMP Phosphodiesterases/antagonists & inhibitors ,5'-Cyclic-GMP Phosphodiesterases/antagonists & inhibitors ,Laboratory Science - Extended Report ,Pigment Epithelium of Eye ,Cyclic GMP ,Cells, Cultured ,In Situ Hybridization ,Cultured ,Inbred Lew ,Pigment Epithelium of Eye/drug effects ,Phosphodiesterase ,Sensory Systems ,medicine.anatomical_structure ,Type 5 ,Retina/drug effects ,Phosphodiesterase Inhibitors/pharmacology ,Cyclic Nucleotide Phosphodiesterases ,medicine.medical_specialty ,Cells ,Phosphodiesterase 3 ,Biology ,Retina ,Cellular and Molecular Neuroscience ,3',5'-Cyclic-GMP Phosphodiesterases ,Internal medicine ,Guanosine monophosphate ,medicine ,3' ,Animals ,Humans ,RNA, Messenger ,Cyclic Nucleotide Phosphodiesterases, Type 5 ,Retinal pigment epithelium ,Phosphoric Diester Hydrolases ,5'-Cyclic-AMP Phosphodiesterases/antagonists & inhibitors ,Phosphoric Diester Hydrolases/genetics ,Molecular biology ,eye diseases ,Rats ,Ophthalmology ,Cyclic GMP/metabolism ,Endocrinology ,chemistry ,Cell culture ,3',5'-Cyclic-AMP Phosphodiesterases ,Rats, Inbred Lew ,RNA ,Messenger/genetics ,sense organs ,PDE10A ,Soluble guanylyl cyclase ,3',5'-Cyclic-GMP Phosphodiesterases/antagonists & inhibitors - Abstract
Aim: To investigate which phosphodiesterase (PDE) is involved in regulating cyclic 3′5′ guanosine monophosphate breakdown in retinal pigment epithelium (RPE) cells. Methods: cGMP content in the cultured RPE cells (D407 cell line) was evaluated by immunocytochemistry in the presence of non-selective or isoform-selective PDE inhibitors in combination with the particulate guanylyl cyclase stimulator atrial natriuretic peptide (ANP) or the soluble guanylyl cyclase stimulator sodium nitroprusside (SNP). mRNA expression of PDE2, PDE5 and PDE9 was studied in cultured human RPE cells and rat RPE cell layers using non-radioactive in situ hybridisation. Results: In the absence of PDE inhibitors, cGMP levels in cultured RPE cells are very low. cGMP accumulation was readily detected in cultured human RPE cells after incubation with Bay60–7550 as a selective PDE2 inhibitor, sildenafil as a selective PDE5 inhibitor or Sch51866 as a selective PDE9 inhibitor. In the presence of PDE inhibition, cGMP content increased markedly after stimulation of the particulate guanylyl cyclase. mRNA of PDE2,PDE5 and PDE9 was detected in all cultured human RPE cells and also in rat RPE cell layers. Conclusions: PDE2, PDE5 and PDE9 have a role in cGMP metabolism in RPE cells.
- Published
- 2006