1. Identifying a piecewise affine signal from its nonlinear observation -- application to DNA replication analysis
- Author
-
Lage, Clara, Pustelnik, Nelly, Audit, Benjamin, Arbona, Jean-Michel, and Gribonval, Rémi
- Subjects
Mathematics - Optimization and Control ,46N10 - Abstract
DNA replication stands as one of the fundamental biological processes crucial for cellular functioning. Recent experimental developments enable the study of replication dynamics at the single-molecule level for complete genomes, facilitating a deeper understanding of its main parameters. In these new data, replication dynamics is reported by the incorporation of an exogenous chemical, whose intra-cellular concentration follows a nonlinear function. The analysis of replication traces thus gives rise to a nonlinear inverse problem, presenting a nonconvex optimization challenge. We demonstrate that under noiseless conditions, the replication dynamics can be uniquely identified by the proposed model. Computing a global solution to this optimization problem is specially challenging because of its multiple local minima. We present the DNA-inverse optimization method that is capable of finding this global solution even in the presence of noise. Comparative analysis against state-of-the-art optimization methods highlights the superior computational efficiency of our approach. DNA-inverse enables the automatic recovery of all configurations of the replication dynamics, which was not possible with previous methods., Comment: 25 pages, 11 figures
- Published
- 2024