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Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Tropical Meteorology (1004-4965) is the property of Guangzhou Institute of Tropical & Oceanic Meteorology, China Meteorological Administration and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This work is to clone the genes of glycosyltransferases UGTPg17 and UGTPg36 from Lonicera macranthoides, and analyze the correlation between their expression and saponin content at different flowering stages. According to the Unigene sequence of L. macranthoides transcriptome, specific primers were designed to clone UGTPg17 and UGTPg36. The physical and chemical properties, protein structure and evolutionary relationship of the cloned UGTPg17- and UGTPg36-encoding proteins were analyzed using the bioinformatics websites; quantitative reverse-transcription polymerase chain reaction(RT-qPCR)was used to analyze gene expressions at different flowering stages. The content of L. macranthoides saponin was determined by HPLC; and the correlation between gene expression and saponin content was analyzed by SPSS. The open reading frame length of UGTPg17 and UGTPg36 genes was 1 410 bp and 1 428 bp, respectively, encoding 469 and 475 amino acids, containing 4 and 7 glycosylation sites, respectively, which belonged to unstable and hydrophilic proteins without transmembrane region. UGTPg36 protein did not contain signal peptide sequence, and the average S value of UGTPg17 protein was >0.5, suggesting that it may contain signal peptide. The expressions of UGTPg17 and UGTPg36 in different flowering stages showed an upward trend, and the saponin content fluctuated in different flowering stages. The correlation analysis between the two showed that the two UGT genes were significantly negatively correlated with the saponin content. In this study, UGTPg17 and UGTPg36 genes were successfully cloned from L. macranthoides, and their expressions variedt at different flowering stages. Correlation analysis showed that UGTPg17 and UGTPg36 genes were related to saponin biosynthesis of L. macranthoides, which lays a foundation for further exploring their specific functions. [ABSTRACT FROM AUTHOR]

The present study identifies 50 MJO cases by using Madden Julian Oscillation (MJO) tracking method and TRMM precipitation, ERA-Interim temperature, specific moisture, and circulation data. The results show that 14 MJO events occurred in El Niño years, and 25 MJO events occurred in La Niña years. Moreover, most MJOs vanished in the western Pacific in La Niña years, while they could further propagate into the eastern Pacific and vanish there in El Niño years. The much shorter propagation range of MJO in La Niña years was due to the lack of moisture accumulation to the east of the MJO convective center when MJO propagated into the western Pacific. The MJO intensified in the Indian and Pacific Ocean in El Niño years due to the stronger moisture transport from the east to the MJO convective center. The larger amount of the moisture transport was attributed to the MJO zonal wind acting on the background moisture in El Niño years. In La Niña years, the MJO intensity was slightly stronger over the MC. [ABSTRACT FROM AUTHOR]