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Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To screen the key genes related to the prognosis, diagnosis, and immune infiltration of the hepatocellular carcinoma (HCC) patients by bioinformatics analysis methods, and to analyze their potential therapeutic drugs. Methods: The HCC gene expression profile data and corresponding clinical informations of the HCC patients were downloaded from the Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database. The R software package limma was used to screen the differentially expressed genes (DEGs) in HCC. Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed on the DEGs. The STRING database was used to construct the protein-protein interaction (PPI) network; the Cytoscape software was used to visualize the PPI network and screen the key genes; Kaplan-Meier survival curve and LASSO regression algorithm were used to identify the key genes related to the HCC prognosis; external data sets were used to validate their expressions and analyze the diagnostic efficacy; CIBERSORT algorithm was used to detect the relationship between the expression of prognosisrelated key genes and HCC immune cell infiltration. The MiRNet and Network Analyst databases were used to construct the microRNA (miRNA)-key gene mRNA and transcription factors (TFs)-key gene mRNA molecular regulatory networks; CMap database was used to screen the potential small molecule drugs for HCC treatment. Results: A total of 146 DEGs were screened, including 30 upregulated genes and 116 downregulated genes. The GO functional enrichment analysis and KEGG pathway enrichment analysis results showed that the DEGs were significantly enriched in biological processes (BP) such as steroid, alkene compound, and hormone metabolism, as well as signaling pathways such as retinol metabolism, drug metabolism-cytochrome P450(CYP450), complement and coagulation cascades. The PPI network analysis identified 14 key genes, among which formimidoyltransferase cyclodeaminase (FTCD), secreted phosphoprotein 2 (SPP2), thrombin-antithrombin complex (TAT), complement C6 (C6), and cytochrome CYP450 family member 2C9(CYP2C9) were significantly associated with the prognosis, clinical pathological stage, and histological grade of the HCC patients and also had high diagnostic efficacy for HCC and were closely related to immune cell infiltration in HCC. Hsa-mir-182-5p, CUT-like homeobox 1(CUX1), early growth response 1(EGR1), SMAD family member 4(SMAD4), and tumor protein P53(TP53) were identified as the important regulators targeting the above-mentioned prognosis-related key genes. DL-thiorphan, promethazine, and apigenin may have the therapeutic effects on HCC. Conclusion: FTCD, SPP2, TAT, C6, and CYP2C9 may be the potential targets for the diagnosis, prognosis, and treatment of HCC. Three predicted small molecule drugs, DL-thiorphan, promethazine, and apigenin, may provide the references for the development of therapeutic drugs for HCC. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transactions of the Chinese Society of Agricultural Engineering is the property of Chinese Society of Agricultural Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to further study the antitumor mechanism of curcumol and its potential clinical application, a SYBR Green real-time polymerase chain reaction(RT-PCR)method was used to analyze the differential expression profiles of 81 human senescence-related genes in human hepatocarcinoma HepG2 cells treated with curcumol. The results showed that the expression of TP53 and its downstream genes p16Ink4a, p21Waf1 / Cip1 and p27Kip1 were significantly up-regulated, accompanied by transactivation of other senescence signaling pathway related genes or senescence response genes such as ABL1, ALDH1A3, CHEK2, HRAS, PTEN, etc., while the expression of Cyclin A2, IGFBP3, SIRT1 and TERT, the genes which negatively regulated cell cycle progression and senescence signaling, were significantly down-regulated. Western blotting verified that protein levels of p53 and its downstream CKIs, p21WAF1 and p16INK4 increased while Cyclin A2 decreased, consistent with the findings in PCR results. The level of wild-type p53-induced protein phosphatase 1(Wip1)was also found significantly increased, suggesting that the induction of senescence in HepG2 cells by curcumol might be through activation of p53 signaling pathway. The present study further demonstrates that curcumol is capable of inducing cellular senescent phenotype in HepG2, accompanying with cell cycle G0 / G1 phase arrest. [ABSTRACT FROM AUTHOR]

Copyright of Research & Exploration in Laboratory is the property of Research & Exploration in Laboratory and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)