[Objective]To identify the sudden death causes of Mauremys mutica in a breeding farm in Shenzhen, Guangdong Province.[Method]A dominant strain named GZWO1 was isolated from the liver, spleen and other focal tissues of the infected Mauremys mutica by the scribing plate separation method. The morphological characteristics, physiological and biochemical characteristics of the isolated strain were identified, and the general primers were used: 16SrDNA sequence was amplified by 27F and 1492R, and the classification status of the pathogen was analyzed by using DNAMAN and MEGA-6.0 software. The pathogenicity of the pathogen was verified by animal regression infection test, and effective treatment and prevention methods were screened by drug sensitivity test.[Result]Strain GZW01 was a rod-like gram-negative bacterium. Its enzyme activity, gluconate test, mannitol, mannose, sucrose, arabinose, inositol, Tanose, MR, VP, arginine decarboxylase and other physiological and biochemical tests were positive, which could produce glucose gas. It had 99% homology with Aeromonas veronii on NCBI. The regression infection test showed that strain GZW01 was highly pathogenic to Tenebrio flavus. The results of drug sensitivity showed that the isolated strain was resistant to penicillin G, benzoxicillin sodium, ampicillin, tetracycline, vancomycin, clindamycin, and a total of six drugs. It was moderately sensitive to clarithromycin; and highly sensitive to 28 antibiotics such as gentamicin, erythromycin, ofloxacin and amtrannan.[Conclusion]Aeromonas veronii was the pathogenic bacterium that caused sudden death of the Mauremys mutica in a breeding farm in Shenzhen City. [ABSTRACT FROM AUTHOR]