Search

目的研究被过表达α-synuclein的SH-SY5Y细胞外泌体刺激后的小胶质细胞重新分泌的外泌体（SNCA-HM Exo）的miRNAs成分变化，及其对神经细胞自噬的影响。方法收集小胶质细胞外泌体进行miRNAs芯片分析和PCR检测，筛选出差异表达的miRNAs，对其靶基因进行KEGG及GO分析，筛选出与PI3K/Akt/信号通路相关的miRNAs。将SH-SY5Y细胞分为四组，分别为Con-HM Exo组，Con-HM Exo+miR-19a mimic组，SNCA-HM Exo组以及SNCA-HM Exo+miR-19a-3p inhibitor组，采用Western Blot进行验证试验。结果芯片分析和PCR共筛选出15个差异表达的miRNAs。KEGG及GO分析发现，其靶基因富集分值最高的是PI3K/Akt信号通路，其中miR-19a-3p调控PI3K/Akt信号通路的靶基因包括PTEN等。验证试验发现，miR-19a 模拟物组及SNCA-HM Exo组与对照组比较PTEN、LC3Ⅱ/Ⅰ下降，p-Akt/Akt、p-mTOR/mTOR、P62升高（P＜0.05），而miR-19a抑制剂可逆转这一作用（P＜0.05）。结论SNCA-HM Exo可通过miR-19a-3p调控PTEN，激活PI3K/Akt/mTOR信号通路，进而抑制神经细胞自噬。

[目的]探讨细胞因子干扰素α（INFα）和甲基化抑制剂5-杂氮脱氧胞嘧啶核苷（5-AZA-CdR）对白血病细胞HL-60和K562的作用机制。[方法]1000U/mL INFα和不同剂量的5-AZA-CdR作用于HL-60和K562 48h,通过RT-PCR检测Xaf1和XIAP mRNA的表达,流式细胞技术检测Bcl-2家族成员、线粒体膜电位（Δψm）和细胞凋亡的情况。[结果]INFα和5-AZA-CdR使HL-60和K562 Xaf1 mRNA表达增加,且与5-AZA-CdR呈剂量依赖性,其中5-AZA-CdR（5μmol/L）的作用最明显;XIAP mRNA表达无变化。INFα使HL-60和K562 Bax表达增加,Bcl-2和Bcl-xl表达无改变;5-AZA-CdR降低HL-60 Bcl-2、Bcl-xl、Bax表达,增加K562 Bax表达,不影响K562 Bcl-2和Bcl-xl。INFα和5-AZA-CdR都能使线粒体膜电位降低、细胞凋亡增加。除细胞凋亡外INFα和5-AZA-CdR对HL-60和K562的Xaf1 mRNA、Bcl-2家族成员以及Δψm无协同作用。[结论]INFα和5-AZA-CdR促进HL-60和K562细胞凋亡,其作用机制可能与上调Xaf1 mRNA的表达,下调Bcl-2（Bcl-xl）/Bax和降低线粒体膜电位有关。

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Nuclear Safety is the property of Nuclear & Radiation Safety Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Advances in Earth Science (1001-8166) is the property of Advances in Earth Science Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Feed Industry is the property of Liaoning Provincial Institute of Agricultural Mechanization and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the semen quality of 3 400 cases of men preparing for pregnancy in a tertiary hospital in Northwest China, and understand the overall situation and changing trends of semen quality in men in Northwest China・ Methods A total of 3 400 men preparing for pregnancy who attended the First Affiliated Hospital of Xinjiang Medical University for semen quality examination from March 2020 to July 2023 were selected as the research subjects. Their sperm concentration, viability, total number of sperms, and other indexes were examined by computer-assisted sperm automated analysis system (CASA)・ The subjects were grouped according to year, age, season} and time of abstinence, and their relationships with semen quality were analysed. Results Total of 3 400 men preparing for pregnancy were included in the study. Men with normal semen quality accounted for 45・ 8% and men with abnormal semen quality accounted for 54> 2% ・ Abnormal parameters in descending order were as follows sperm concentration, total number of sperm, forward motile sperm rate, total sperm viability, and semen volume. The proportion of men in the age group of 27-39 years old who underwent semen quality testing was larger, and the total number of sperm, forward motile sperm rate, and total sperm viability varied significantly between ages. Vitality varied between ages, with a significant decline in each indicator in the group with age 3 40; Male semen quality showed a downward trend from year to year. Seasons had obvious effect on semen quality, with a significant decrease in semen quality in the summer months. There was a linkage between the duration of abstinence and semen quality, and performing semen quality tests was preferable within three to seven days of abstinence. Conclusion The main abnormal indicators of semen quality for men in Xinjiang are spenn concentration, total sperm count and forward-moving sperm rate, and age is negatively correlated with each semen parameter, so it is recommended to complete childbearing before the age of 40. [ABSTRACT FROM AUTHOR]

Copyright of Arid Land Geography is the property of Chinese Academy of Sciences, Xinjiang Institute of Ecology & Geography and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To explore the mechanism of chitosan oligosaccharides(COS) in reducing atherosclerotic plaque formation from the perspective of protein glycosylation modification. Methods Totally 40 ApoE-/- mice were randomly divided into control group and COS group. The control group was given a high-fat diet for 12 weeks, and COS group was given a high-fat diet plus COS(gavage per day, 500 mg/kg) for 12 weeks. Serum lipid detection, HE staining and Oil red O staining were used to detect plaque formation. Lectin chip, liquid chromatography tandem-mass spectrometry and ELISA were used to detect potential changes of glycoprotein in serum. Cholesterol ester outflow and free cholesterol ester determination experiment were used to evaluate the effect of changes in scavenger receptor class B type Ⅰ(SRBI) protein glycosylation modification site on cholesterol effluence in macrophages. Results COS significantly reduced the level of TC and LDL-C(P＜0.05) in mice, but had no effect on the level of TG,HDL-C,ApoA1 and ApoB100. The intima thickness and plaque size of the aorta were significantly thinner and smaller(P＜0.05)in the COS group compared with the control group. The molecular weight of lens culinaris agglutinin(LCA)binding protein with the most obvious change is 80-90 ku, and SRBI was one of them. COS promoted the cholesterol outflow and inhibited the accumulation of free cholesterol ester in RAW264.7 cell(P＜0.05). Knockdown or glycosylation site mutation with SRBI inhibited cholesterol outflow caused by COS, and increased the accumulation of intracellular free cholesterol(P＜0.05). Conclusions COS promotes lipid efflux by increasing SRBI glycosylation and expression, thereby alleviating atherosclerotic plaque formation. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Cancer Biotherapy is the property of Editorial Office of Chinese Journal of Cancer Biotherapy and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bulletin of Soil & Water Conservation is the property of Bulletin of Soil & Water Conservation Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the influencing factors for death within 30 days in patients with decompensated hepatitis B cirrhosis and hepatic encephalopathy. Methods A retrospective analysis was performed for 616 patients with hepatitis B cirrhosis and hepatic encephalopathy in Beijing Ditan Hospital from January 2008 to April 2018, and all patients were followed up for 30 days. According to their prognosis, they were divided into survival group with 488 patients and death group with 128 patients. The MannWhitney U test was used for comparison of continuous data between two groups, and the chi-square test or the Fisher’s exact test was used for comparison of categorical data between two groups. The Cox regression analysis was used to investigate the independent risk factors for death within 30 days in patients with hepatitis B cirrhosis and hepatic encephalopathy. Results The multivariate Cox regression analysis showed that age (hazard ratio ［HR］=1.029, 95% confidence interval ［CI］：1.014—1.044, P<0.001), Model for End Stage Liver Disease (MELD) score (HR=1.118, 95%CI：1.098—1.139, P<0.001), and neutrophil-to-lymphocyte ratio (NLR) （HR= 1.036, 95%CI：1.015 — 1.057, P=0.001) were independent risk factors for death within 30 days in patients with hepatitis B cirrhosis and hepatic encephalopathy. The stratified analysis showed that the patients with a MELD score of≥20 and an NLR of≥4 had a higher risk of death, with a 30-day mortality rate of 57.1% (80/140) . The patients with a MELD score of<20 and an NLR of<4 had a 30- day mortality rate of 3.9% (9/232) . Conclusion Age, MELD score, and NLR are independent risk factors for death within 30 days in patients with hepatitis B cirrhosis and hepatic encephalopathy, and patients with a MELD score of≥20 and an NLR of≥4 tend to have a high risk of death. [ABSTRACT FROM AUTHOR]

Copyright of Ordnance Industry Automation is the property of Editorial Board for Ordnance Industry Automation and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Science & Technology (0253-2336) is the property of Coal Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Sedimentologica Sinica is the property of Acta Sedimentologica Sinica Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

高血压作为心血管疾病的重要危险因素之一, 其发病率、致残率及致死率居高不下。高血压是多因素作 用的结果, 发病机制尚不完全明确。肠道微生态作为人体内环境的重要部分, 被认为与高血压有关。高血压影响肠 道菌群组成、分布及肠屏障的特异性表达, 肠道微环境产生短链脂肪酸、氧化三甲胺、类固醇激素、胆汁酸等代谢产 物, 最终通过介导免疫炎症、肾素-血管紧张素-醛固酮系统、自主神经系统、氧化应激、受体作用等方面实现肠道-高 血压的交流通讯。因此, 靶向肠道菌群的益生菌、高纤维饮食、中医中药对防治高血压有重要意义。 [ABSTRACT FROM AUTHOR]

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Practical Medicine / Shiyong Yixue Zazhi is the property of Journal of Practical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective　To obtain the optimal extraction process of narciclasine from Hymenocallis littoralis by response surface center design. Methods　Based on the single factor experiment, temperature, time and liquidto-solid ratio were selected to establish a mathematical model combined with the Box-Behnken test. The influence degree of the three factors on the extraction rate of narciclasine was analyzed and investigated to determine the optimal extraction conditions. Results　The optimum extraction conditions were as follows: extraction temperature 90.97 ℃, extraction time 4.13 h, liquid-solid ratio 5.09 mL∶1 g. The predicted and verified values of narciclasine extraction were 3.42×10-4% and 3.38×10-4%, respectively. Conclusion　The optimized extraction process based on the response surface method is stable, feasible, and predictable, which could be used for the extraction of active components from Hymenocallis littoralis, and has laid the groundwork for the development and utilization of Hymenocallis littoralis. [ABSTRACT FROM AUTHOR]

大叶蒟 (Piper laetispicum)是胡椒科胡椒属植物. 大叶蒟根茎提取物的主要成分有酰胺生物碱类化合物、 吡咯烷生物碱类化合物、黄酮类化合物及甾醇类化合物, 酰胺生物碱类化合物包括大叶蒟素、大叶蒟酰胺A、大叶蒟 酰胺B、酰胺生物碱等, 吡咯烷类化合物包括5'-甲氧基-3', 4'-次甲二氧基桂皮酸四氢吡咯、1[ (- 2E, 4E)-9 (- 3', 4'-次 甲二氧基苯)七碳二烯酰] 四氢吡咯及1[ (- 2E, 7E)-N-7 (- 3', 4'-次甲二氧基苯)九碳二烯酰] 四氢吡咯等, 黄酮类化合 物包括胡椒醇、d-芝麻素、牡荆苷等, 甾醇类化合物包括β-谷甾醇、豆甾醇及匙叶桉油烯醇等. 大叶蒟根茎挥发油化 学成分包括反式-石竹烯、δ-荜澄茄烯及γ-古芸烯等萜烯类化合物. 大叶蒟素、大叶蒟乙酸乙酯提取物及3 (- 3', 4'- 亚甲基二氧基-5-三氟甲基苯基)-2E-丙烯酸异丁基酰胺具有抗抑郁作用, 反式-石竹烯具有镇静、镇痛及抗氧化 作用. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Occupational Health & Emergency Rescue is the property of Occupational Health & Emergency Rescue Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Science & Technology (0253-2336) is the property of Coal Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Scientiarum Naturalium Universitatis Sunyatseni / Zhongshan Daxue Xuebao is the property of Sun-Yat-Sen University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Medical Education Technology is the property of China Medical Education Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Ordnance Industry Automation is the property of Editorial Board for Ordnance Industry Automation and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Engineering for Thermal Energy & Power / Reneng Dongli Gongcheng is the property of Journal of Engineering for Thermal Energy & Power and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electric Power Engineering Technology is the property of Editorial Department of Electric Power Engineering Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Railway Science & Engineering is the property of Journal of Railway Science & Engineering Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Science & Technology (0253-2336) is the property of Coal Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Plastics / Zhongguo Suliao is the property of Journal Office of CHINA PLASTICS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Polymer Materials Science & Engineering is the property of Sichuan University, Polymer Research Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)