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Objective Investigation of the effect of aqueous extract of Jiangu Decoction on osteoclast differentiation and osteoblast proliferation and differentiation. Methods Preparation of JGF aqueous extract. MTT method was applied to study the toxicity of Jiangu Decoction on bone marrow mononuclear macrophages (BMMs) cells. The differentiation of BMMs into osteoclasts was induced by using nuclear factor-KB receptor activating factor ligand (RANKL), and different concentrations of the drug were added. Anti-tartrate acid phosphatase (TRACP ) staining was used to determine the inhibition of osteoclastogenesis of Jiangu Decoction. The signaling pathway of NF-κB osteoclast differentiation induced by RANKL was determined by Western blot, and the RT-qPCR method was used to determine the mRNA expression levels of NFATc1, C-FOS, etc., which are key genes of osteoclastogenesis downstream of the signaling pathway. MC3T3-El cells were used as precursor osteoblasts, and different concentrations of drugs were added for intervention. The proliferation ability of cells was measured by the CCK8 method . Alkaline phosphatase (ALP) activity was detected by the PNPP method, and the mineralization ability of cells was measured by alizarin red S staining. Results MTT assay showed that the cytotoxic concentration of Jiangu Decoction was greater than (P < 0. 05) 500 μ,g/mL, and the IC50 of osteoclastogenesis inhibition was 1. 25 μg/mL, Mechani stic studies showed that Jiangu Decoction significantly downregulated the protein expression of p-P65 and P53 in the RANKL-NF-κB signaling pathway (P< 0. 05) significantly inhibited the downstream of the pathway C-FOS, NFA TC, etc. mRN A expression levels downstream of the pathway (P < 0. 01, P < 0. 05). In addition, the osteoblast activity assay showed that Jiangu Decoction could significantly promote the proliferation ability of MC3T3-El cells, improve ALP activity and increase the calcification ability of osteoblasts. Conclusion JGF has the pharmacological effect of inhibiting osteoclast differentiation and promoting osteogenic precursor cell proliferation, differentiation, and mineralization. Its osteoclast inhibitory effect was associated with inhibition of the RANKL-NF-κB signaling pathway and its downstream expression of C-FOS, NF ATC 1, etc. genes, and osteoblast differentiation promotion was associated with upregulation of CALI A2, SPARC, and FOSL1 gene expression. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)