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近年来随着我国城镇化进程的持续推进，仍然需要建造大量房屋。传统的建造方式质量差、效率低、污染大，且对劳动力依赖程度高，因此房屋建造方式急需升级，工业化生产、装配式施工、绿色环保的建造方式应运而生。装配式钢结构建筑天然具有绿色装配的特点，还可以化解钢铁产业过剩产能，是最理想的住宅之一，近年来在国家政策的倡导下取得了较大的发展。针对装配式钢结构建筑的特点，列出了11种装配式钢结构建筑结构体系，并对每种结构体系的优缺点、研究现状及应用进行了总结归纳，在已有研究的基础上指出目前装配式钢结构建筑体系发展中存在的问题。

【目的】检测缺氧诱导因子-1（HIF-1）在兔结膜伤口愈合模型的表达水平，并研究其在结膜瘢痕形 成中的作用。【方法】48只新西兰白兔根据不同观察时间点（术后12h和1、 3、 5、 7d以及空白对照组）随机分为 6组。对所有实验组动物构建低位结膜瓣，术后观察术区并评估结膜血管化水平。实时荧光定量PCR和蛋白免 疫印迹（Westernblot）检测术区HIF-1α的表达水平，根据病理切片评估术区结膜纤维化水平。【结果】Western Blot结果显示HIF-1α蛋白表达水平在术后第3天（P＜0.001）和第5天（P＜0.001）均高于空白对照组。在术后不 同时间点，HIF-1α的mRNA水平的变化趋势与HIF-1α的蛋白水平的变化趋势相一致。术后结膜血管化水平与 HIF-1α的蛋白水平呈正相关关系（r=0.626；P＜0.001）。此外，术后第3天［（37.88±6.88）%；P=0.006］、第5 天［（47.50±6.26）%；P＜0.001）］和第7天［（53.45± 3.78）%；P＜0.001）］的胶原纤维含量高于空白对照［（23.61 ±5.26）%］。【结论】构建兔结膜伤口愈合模型后，HIF-1α在兔结膜和Tenon’ s囊组织存在表达。HIF-1α过表 达可能促进兔结膜伤口愈合。

文章对带有斜端面光纤结构的改进型同轴探测器的反射机理进行了简要计算,并在此基础上运用高斯光学理论对该结构的反射光路进行了理论分析。在排除物理接触面的反射因素后,对该结构样品进行了测试,测试结果和所作的理论分析结果基本一致。实验结果说明该理论分析可以应用于实践。

[目的]探讨皮肤干细胞诱导分化为结膜上皮细胞,为组织工程结膜探索一种新型的种子细胞,为严重眼表疾病的治疗奠定基础。[方法]在体外培养纯化鉴定恒河猴皮肤干细胞,并用Hoechst 33342标记,标记后的皮肤干细胞与结膜上皮细胞进行Transwell非接触共培养10d,然后对诱导后的细胞用细胞免疫化学以及流式细胞术检测结膜上皮细胞的特异性表面标志,包括角蛋白4和粘蛋白4,同时检测细胞的Hoechst 33342表达情况。[结果]纯化后的皮肤干细胞比例接近90%。Hoechst 33342标记后的皮肤干细胞胞核显示蓝色荧光。在与结膜上皮细胞共培养10d后,皮肤干细胞显示结膜上皮细胞的标志粘蛋白4和角蛋白4阳性,流式细胞术检测阳性细胞比例分别为49.9%和95.6%,同时细胞还显示胞核的Hoechst 33342表达。[结论]灵长类动物恒河猴的皮肤干细胞,在体外的适宜条件下,可诱导分化为有结膜上皮细胞特征的细胞。

【目的】观察人骨髓间充质干细胞（mesenchymal stem cells，MSCs）在保存人羊膜及兔眼表结膜基质上分化的情况。【方法】24只新西兰兔随机分为2组。实验组：培养有人MSCs的羊膜移植组，将人MSCs接种在保存人羊膜上培养4d，用5-溴脱氧尿嘧啶（BrdU）标记后移植到兔眼表结膜缺损区；对照组，单纯羊膜移植组。分别于移植后1，2，3，4，6和8周，摘取各组实验眼行组织学和免疫组织化学检查，组织学检查移植到兔结膜基质的MSCs的存活、形态变化以及移植局部的反应等情况；免疫组织化学检测移植到结膜基质的带有BrdU标记的细胞角蛋白3／12（CK3／CKl2）和角蛋白13（CK13）的表达。【结果】MSCs接种到羊膜后能在羊膜上生长，与羊膜共培养4d后，MSCs贴附羊膜生长迅速，组织学特征无明显改变。用羊膜负载MSCs移植到兔结膜缺损区，术后免疫组织化学检测结膜上皮层CK13表达阳性，CK3／CK12表达阴性，在重建的结膜上皮层可检测到BrdU核阳性细胞，未见异常增殖细胞。【结论】人羊膜可作为MSCs的载体，采用人羊膜负载MSCs行兔结膜移植，MSCs能在兔结膜上皮层存活并增殖。

【目的】探讨羟基磷灰石板对矫正眼眶爆裂性骨折的作用及手术方法。【方法】23 例病人, 男18 例, 女性5 例, 先依眼眶CT 扫描确定骨折位置及眼眶组织的嵌顿情况, 再从骨折位置的骨膜下探查并把嵌顿的软组织复位, 然后, 用2 mm 厚的羟基磷灰石板从骨膜下填充骨折凹陷区。手术过程注意避免损伤眶内的神经和血管等重要组织, 对合并眶缘骨折缺损 者用钛网成形。手术前后常规测量眼球突出度, 对视力高于0.1 者行同视机检查, 术后平均随访18.2 个月。【结果】所有病 人眼球内陷得到不同程度的矫正, 18 例(78.3 %)从术前双眼球突出度相差4 ～ 10 mm 恢复至术后相差

【目的】评价先天性睑裂狭小综合征的手术治疗效果。【方法】对 50 例先天性睑裂狭小综合征, 采用改良 Y-V 矫 正联合内眦韧带缩短法矫正内眦赘皮,缩短内眦间距,3 个月后二期行额肌瓣悬吊术矫正上睑下垂。【结果】经随访 6 个月～ 5 年, 证实能达到矫正内眦赘皮,延长睑裂 ,缩短内眦间距和充分上提上睑的目的, 治疗效果满意。【结论】改良 Y-V 矫正联合 内眦韧带缩短法和额肌瓣悬吊术是治疗先天性睑裂狭小综合征的效果确切、简便又安全的手术方法。

目的】分析多孔羟基磷灰石(HA)义眼台眶内植入后的并发症并探讨其原因。【方法】回顾眶内植入羟基磷灰石 义眼台320 例, 随诊6 月～ 4 年。【结果】320 例中发生并发症的有46 例(14.4 %), 其中义眼台暴露41 例(12.8%), 义眼台位置 异常2 例, 其它并发症包括义眼台慢性感染、结膜肉芽肿等。义眼台暴露的发生时间为术后7 d～ 6 月, 范围为2 ～ 15 mm 。【结 论】羟基磷灰石义眼台眶内植入最常见的并发症是义眼台暴露, 并发症的发生原因与多种因素有关, 包括术前眼病史、包裹义 眼台的异体巩膜的排斥溶解、义眼台的选择、缝合技术、术后眼眶的放射治疗等。

面对移动通信网络层出不穷的安全问题和潜在的安全隐患,移动通信网络病毒防治工作已成为非常重要的课题。文章主要通过真实案例说明当前移动通信网络的安全危机,以引起移动通信用户对手机病毒的防范意识;同时,文章还描述了移动通信网络安全系统的解决方案,即同时从终端侧和网络侧采取预防应对措施。

文章较为详细地介绍了高回损同轴封装接收组件关于回波损耗指标的定义和指标要求,根据这些指标要求展开了分析,并提出了能达到指标要求的可插拔式接收组件（ROSA）的设计原理;同时介绍了武汉电信器件有限公司在这方面所做的工作以及样品的实验结果。研制样品的测试结果表明研制是成功的。

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Copyright of Journal of Engineering Management / Gongcheng Guanli Xuebao is the property of Journal of Engineering Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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In the intelligent diagnosis of pancreatic lesions by ultrasonic endoscopy, a diagnostic model based on the twin network architecture was proposed to address the problems of misdetection, omission and low accuracy in the detection of pancreatic lesions in different age groups and genders, as well as the detection of pancreatic lesions in multiple sites. The model consists of two improved ConvNeXt models, which perform the task of pancreatic site classification and the task of locating cancerous areas without labelling the cancerous sites with Grad_CAM, respectively. The improved ConNeXt model uses feature fusion to combine detailed texture and abstract semantic information, and the fusion ratio relationship of feature vectors is solved through experimental exploration to improve the classification accuracy and stability of the model. Experiments show that the twin ConvNeXt network model improved by feature fusion achieves a classification accuracy of 98. 34% for pancreatic sites and up to 99. 47% for cancerous sites, and the activation regions generated by Grad_CAM are highly consistent with the real cancerous regions, which provides an effective auxiliary diagnosis for doctors. [ABSTRACT FROM AUTHOR]

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Objective To analyze the expression changes of leucine-rich repeat containing 15（LRRC15） and Sad1 and UNC84 domain protein 2（SUN2） in epithelial ovarian cancer （EOC） tissues and to investigate their relationships with the prognosis of patients. Methods Totally 102 cases of cancer tissues resected from patients with EOC were selected as the observation group， and 60 cases of normal ovarian tissues from patients who underwent surgical treatment due to benign ovarian cysts were used as the control group. The expression levels of LRRC15 and SUN2 in the cancer and normal ovarian tissues were detected by immunohistochemistry. The correlation between the expression levels of LRRC15 and SUN2 was evaluated by Spearman rank correlation. The relationships between the expression levels of LRRC15 and SUN2 and the prognosis of patients with EOC were analyzed by Kaplan-Meier curve and Cox regression analysis. Results The positive rate of LRRC15 in the observation group was higher than that in the control group, and the positive rate of SUN2 was lower than that in the control group, with statistically significant differences （both P<0. 05）. In the observation group, the expres‐ sion of LRRC15 was negatively correlated with the expression of SUN2（r=–0. 634，P<0. 05）. The positive rate of LR‐ RC15 in EOC patients with FIGO stage Ⅲ and lymph node metastasis was higher than that in cancer tissues with FIGO stages Ⅰ-Ⅱ and without lymph node metastasis, and the positive rate of SUN2 in EOC patients with FIGO stage Ⅲ and lymph node metastasis was lower than that in cancer tissues with FIGO stages Ⅰ-Ⅱ and without lymph node metastasis, with statistically significant differences （all P<0. 05）. The 3-year overall survival rate of EOC patients in the LRRC15 positive group was lower than that in the LRRC15 negative group, and the 3-year overall survival rate of EOC patients in the SUN2 negative group was lower than that in the SUN2 positive group, with statistically significant differences （all P<0. 05）. FIGO stage Ⅲ, pathological grade Ⅲ， lymph node metastasis, and positive LRRC15 were risk factors for the prognosis of EOC patients, and positive SUN2 was a protective factor. Conclusions The expression level of LRRC15 increases and the expression level of SUN2 decreases in the cancer tissues of EOC patients. The expression levels of LRRC15 and SUN2 are related to the tumor progression of EOC. Detection of the levels of the two is helpful for evaluating the prognosis of EOC patients. [ABSTRACT FROM AUTHOR]

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Objective: To use pan-genomics and subtractive proteomics techniques to screen the new antibacterial targets from the Staphylococcus aureus genome, and to lay the foundation for the development of anti-Staphylococcus aureus drugs. Methods: The genome sequencing data of 50 strains with sequencing level Complete were collected by searching the whole genome sequencing data in the National Center for Biotechnology Information (NCBI) Database with Staphylococcus aureus as the keyword; BPGA tool was used to conduct the pan-genomics analysis on the genomic data to obtain the core genes of Staphylococcus aureus; subtractive proteomics technique was used to screen the potential antibacterial targets from the core genes. These potential antibacterial targets were used as the receptors; LibDock software was used to screen the potential anti-Staphylococcus aureus drugs from the US Food and Drug Administration (FDA)-approved drug library; molecular docking technology was used to analyze the binding abilities of the drugs and targets. Results: There were 14 379 gene families in the 50 Staphylococcus aureus genomes, of which 1 620 were the core genes. The subtractive proteomics analysis results showed that tyrosine autokinase 1335 was the potential anti-Staphylococcus aureus target. LibDock software screened out nine compounds, including balofloxacin, tenofovir disoproxil fumarate, and adefovir, that may exert anti-Staphylococcus aureus effects on this target protein. The molecular docking results showed there was good binding abilities between the targets and the compounds. Conclusion: Tyrosine autokinase may be the potential target for anti-Staphylococcus aureus. [ABSTRACT FROM AUTHOR]

[Objective] Cytochrome P450 monooxygenase 98（CYP98）is a key rate-limiting enzyme in the phenylpropanoid pathway, and it is proposed to investigate whether it is involved in the biosynthesis of the phenylpropanoid pathway and in the anti-disease function in Hornworts, so as to provide a reference for the future study of the evolution of the early terrestrial plants and physiological mechanisms of adaptation to adversity stress. [Method] The full-length sequence of FfCYP98 cDNA was cloned from Folioceros fuciformis using RACE, analyzed for bioinformatics and subcellular localization, and functionally verified by constructing an overexpression vector to transform the Arabidopsis thaliana mutant FfCYP98-OE1. [Result] The FfCYP98 cDNA sequence, with an open reading frame of 1305 bp, is evolutionarily closest to the Bryophytes Anthoceros angustus and Physcomitrium patens CYP98, and subcellular localization results show that FfCYP98 is localized to both the nucleus and the cytoplasm. The overexpression of the FfCYP98 gene revealed that the expressions of total phenolics, total flavonoids, and C4H,4CL and C3H in the phenylpropanoid pathway was significantly higher in FfCYP98-OE1 plants than in A. thaliana WT plants. In addition, infestation of F. fuciformis and A. thaliana FfCYP98-OE1 plants with Botrytis cinerea revealed that the expression of FfCYP98 was significantly up-regulated, that FfCYP98-OE1 plants died significantly slower than WT plants, and that the expressions of total phenolics, total flavonoids, and four genes, C4H, HCT, C3H, and CAD in the phenylpropanoid pathway were significantly higher in A. thaliana FfCYP98-OE1 plants than in WT plants. [Conclusion] FfCYP98 may be involved in the biosynthesis of phenolics and flavonoids in the phenylpropanoid pathway by regulating the expressions of genes related to the synthesis of phenylpropanoid pathway, and may be related to disease resistance of plants. [ABSTRACT FROM AUTHOR]

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X-rays have high photon energy, which results in a significant penetration depth in ZnO materials. Therefore, in order to detect X-rays effectively, it is necessary to prepare ZnO films with a thickness of micrometers or hundreds of micrometers. In this paper, a 14. 85 μm-thick ZnO film was rapidly prepared by electrochemical deposition method. The experimental results show that the deposition potential and electrode material have a significant effect on the deposition rate and surface morphology of ZnO films. With increasing the deposition potential, the morphology of ZnO changes from hexagonal columnar to lamellar structure. When the potential is too high, a large amount of hydrogen is generated, resulting in an increase in the number of holes in the film that prevent it from forming. When using metal Pt as the anode electrode, the solution will gradually acidify, resulting in the dissolution of ZnO as it is deposited, and when the rates of the two coincide, the ZnO film thickness will no longer increase, making it unsuitable for growing ZnO thick films. In contrast, when using metal Zn as the anode electrode, the pH of the solution remains essentially unchanged, which is more suitable for growing ZnO thick films. A ZnO thick film of 14. 85 μm was grown for 1 h using metallic Zn as the anode electrode with a deposition potential of 0. 65 V and an electrolyte concentration of 0. 4 mol/L. When biased at 5 V, the ZnO thick film based detector has been shown to achieve a responsivity of 66. 8 μC·Gy-1 ·cm-2 to X-rays with an accelerating voltage of 40 kV. [ABSTRACT FROM AUTHOR]

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Livedoid vasculopathy is a type of vascular disorder with unclear etiology and pathogenesis? in addition to the lack of standardized diagnostic and treatment guidelines. Currendy, it is believed that the development of this disease is associated w让h blood hypercoagulability and inflammation. However, studies also suggest the involvement of immune and inflammatory reactions in its pathogenesis. And anti-inflammatory therapy is effective. The drugs used for the treatment of livedoid vasculopathy include plant alkaloids (colchicine, tripterygium glycosides), glucocorticoids, JAK inhibitors, adalimumab, intravenous immunoglobulins and anti-inflammatory drugs. This review aims to summarize the anti - inflammatory therapy and methods for livedoid vasculopathy, providing references for clinicians in the management of this disorder. [ABSTRACT FROM AUTHOR]

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