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[目的]探讨皮肤干细胞诱导分化为结膜上皮细胞,为组织工程结膜探索一种新型的种子细胞,为严重眼表疾病的治疗奠定基础。[方法]在体外培养纯化鉴定恒河猴皮肤干细胞,并用Hoechst 33342标记,标记后的皮肤干细胞与结膜上皮细胞进行Transwell非接触共培养10d,然后对诱导后的细胞用细胞免疫化学以及流式细胞术检测结膜上皮细胞的特异性表面标志,包括角蛋白4和粘蛋白4,同时检测细胞的Hoechst 33342表达情况。[结果]纯化后的皮肤干细胞比例接近90%。Hoechst 33342标记后的皮肤干细胞胞核显示蓝色荧光。在与结膜上皮细胞共培养10d后,皮肤干细胞显示结膜上皮细胞的标志粘蛋白4和角蛋白4阳性,流式细胞术检测阳性细胞比例分别为49.9%和95.6%,同时细胞还显示胞核的Hoechst 33342表达。[结论]灵长类动物恒河猴的皮肤干细胞,在体外的适宜条件下,可诱导分化为有结膜上皮细胞特征的细胞。

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Copyright of Journal of Chongqing University of Technology (Natural Science) is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective·To study the effects of translocator protein (TSPO) agonist Ro5-4864 on autophagy and Kelch-like ECHassociated protein 1 (Keap1)/nuclear factor erythroid-derived-2-like 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling in diabetic neuropathic pain (DNP) rats. Methods·Type 2 diabetic rats were established by high-fat diet and streptozotocin (STZ), and DNP rats were filtered by behavioral assessment. Twenty-four rats were randomly assigned to the Sham group, DNP group, TSPO agonist Ro5-4864 group (Ro group), and TSPO agonist Ro5-4864 combined with Nrf2 inhibitor ML385 group (Ro+ML385 group). Up-Down method was used to measure paw 50% mechanical withdrawal threshold (50% PMWT) of the rats before high-fat diet (baseline), and on Day 3, 7, 14, 21 and 28 after STZ. Sciatic nerves were collected on the last day to analyze the effects of Ro5-4864 on autophagy related proteins and Keap1/Nrf2/HO-1 signaling related proteins of DNP rats by using immunofluorescence and Western blotting. Results·The 50% PMWT in the DNP group decreased from D3 to D28 (P=0.000 at all timing), and the expression of Bcl-2 interacting coiled-coil protein 1 (Beclin-1), microtubule-associated protein light chain 3-Ⅱ (LC3-Ⅱ), HO-1, and nuclear Nrf2 (P=0.000) were significantly reduced in the sciatic nerves of DNP rats (all P=0.000), compared with those in the sham group, but p62 was significantly increased (P=0.000). Administration of Ro5-4864 attenuated these changes in the rats of the Ro group. There was a gradual increase in the 50% PMWT, compared with that of the rats in the DNP group (D14 P=0.039, both D21 and D28 P=0.000), and the impairment of autophagy and the Keap1/Nrf2/HO-1 signaling was repaired, which was demonstrated by increases of Beclin-1, LC3-Ⅱ, HO-1, and nuclear Nrf2 protein contents (all P=0.000) and a decrease in p62 content (P=0.001). However, the beneficial effects of Ro5-4864 were totally abrogated by ML385 in rats of the Ro+ML385 group. Conclusion·TSPO alleviates DNP in rats, of which the mechanism involves activation of autophagy via upregulation of the Keap1/Nrf2/HO-1 signaling in sciatic nerves. This study provides a new strategy for the treatment of DNP. [ABSTRACT FROM AUTHOR]

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To explore the predictive value of rapid sequential organ failure score(qSOFA)combined with serum homocysteine( Hcy) and triacylglycerol(TG) in the short-term prognosis of acute pancreatitis(AP). The 210 AP patients who were admitted to Department of Gastroenterology, the First Affiliated Hospital of Suzhou University from September 2019 to September 2021 were selected as the research objects. According to the Atlanta AP classification guidelines,they were divided into 125 cases of mild AP (MAP group),45 cases of moderate and severe AP(MSAP group)and 40 cases of severe AP(SAP group). The qSOFA score, Hcy and TG levels of the three groups were compared. According to the prognosis 28 days after admission, all patients were divided into 192 cases in the survival group and 18 cases in the death group. Univariate and multivariate logistic regression were used to analyze the prognostic factors of AP patients. The predictive value of qSOFA score, Hcy and TG on the short-term prognosis of AP patients was analyzed by receiver operating characteristic (ROC) curve. The qSOFA score, Hcy and TG levels in SAP group and MSAP group were higher than those in MAP group, and those in SAP group were higher than those in MSAP group (P<0.05). Univariate and multivariate logistic regression analysis showed that higher qSOFA score and higher levels of TG and Hcy were the risk factors of death in AP patients(P<0. 05). The area under the curve (AUC) of qsofa score combined with Hcy and TG to predict the short-term prognosis of AP patients was 0.982, which was significantly higher than 0.715, 0.780 and 0.782 detected by the three indexes alone. qSOFA score, TG and Hcy levels are the influencing factors of short-term prognosis of AP patients, and the combined detection of qsofa score, Hcy and TG levels has high predictive value for the short-term prognosis of AP patients. [ABSTRACT FROM AUTHOR]

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Copyright of Bulletin of the Chinese Academy of Sciences / Chung-kuo ko Hsueh Yuan Yuan Kan is the property of Institutes of Science & Development, Chinese Academy of Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In recent years, the application scope of artificial intelligence in the medical field has gradually expanded and has become one of the hot spots in medical research. With the in-depth research on machine learning, artificial intelligence has achieved rapid development in image detection, face recognition and other aspects, and its performance and accuracy have surpassed human beings in some aspects. The recognition and processing of facial images are extremely important for ophthalmic plastic and reconstructive surgery, and the development of artificial intelligence is an opportunity. This review looks forward to the application direction and future development of artificial intelligence in ophthalmic plastic and reconstructive surgery. [ABSTRACT FROM AUTHOR]