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Copyright of Journal of Shanghai Jiao Tong University (1006-2467) is the property of Journal of Shanghai Jiao Tong University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The protective effects of chitosan-montmorillonite intercalation complex (CS-MMT) on the clinical symptoms, testicular morphology, and sperm quality of heat-stressed male mice were studied. Thirty 6-week-old male ICR mice were randomly divided into control group (CON group), heat stress group (HS group), low-dose CS-MMT group (L group, 50 mg/kg CS-MMT), middle-dose CS-MMT group (M group, 100 mg/kg CS-MMT), and high-dose CS-MMT group (H group, 200 mg/kg CS-MMT). The results showed that compared with CON group, the testicular tissue damage was obvious in HS group, sperm density was significantly decreased (P<0.05), sperm viability and acrosome integrity were extremely decreased (P<0.01), and the sperm malformation and acrosome malformation rates were extremely increased (P<0.01). Compared with HS group, the testicular tissue morphology and structure of mice in CS-MMT groups were significantly improved, the sperm density was increased, the sperm viability and acrosome integrity were extremely increased (P<0.01), and the sperm malformation and acrosome malformation rates were extremely decreased (P<0.01) in a dose-dependent manner. The study indicates that CS-MMT can alleviate the reproductive function damage induced by heat stress in male mice, and and the dosage of 200 mg/kg is better. [ABSTRACT FROM AUTHOR]

Aiming at the cementing problem of long open hole malignant leakage wells, the thermo-sensitive shape memory loss prevention spacer and foam leakage prevention cement slurry were developed, which synergistically reduce the cementing loss risk of long open hole malignant leakage loss wells. The thermo-sensitive shape memory material was developed, which resistance temperature is 150 ℃ and deformation memory temperature is 80～110 ℃, the length of thermo-sensitive shape memory mesh particles after deformation is 10 times that of normal temperature, and the diameter of shape memory expansion ball after expansion is 3 times that of normal temperature. The thermo-sensitive leak-proof spacer was developed, and the bearing-capacity of 3mm crack can reach 8.5MPa. Foaming agent and foam stabilizer were selected, and foam cement slurry were developed, and the density can be adjusted from 1.12 to 1.31 g/cm3, the difference between the upper and lower parts was less than 0.01 g/cm3, and the compressive strength of 1.12 g/cm3 foamed cement stone can reach 6.6 MPa at 30 ℃/72 h, and bubbles in set cement are uniformly dispersed. The spacer duration design method that considering the equivalent plugging time and washing efficiency was established, and the foamed cement slurry section constant gas injection method was formed. Based on thermo-sensitive anti-leakage spacer and foam cement slurry, The leak-proof cementing technology has been applied for 2 Wells, and the one-time sealing section length exceeds 4000 m, and the spacer and cement slurry returned to the surface successfully and got good results. [ABSTRACT FROM AUTHOR]

Copyright of Precious Metals / Guijinshu is the property of Precious Metals Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The aims of this study are to explore the synergistic relationship between deep roots and soil moisture and nutrients in rainfed Medicago sativa L. and Caragana korshinskii land in the loess hilly area of southern Ningxia, and to provide scientific basis for the construction and management of artificial vegetation and the high-quality development of ecological environment in the hilly area of southern Ningxia. [Methods] The changes of soil moisture, root vertical distribution and soil N and P contents in 0—1 000 cm soil layer of 18-year-old Medicago sativa L. land and 20-year-old Caragana korshinskii forest land were analyzed by field investigation and indoor analysis. [Results]（1） The whole profile of soil below 200 cm in Medicago sativa L. land and Caragana korshinskii land was already in a dry state. The soil layers of Medicago sativa L. land and Caragana korshinskii land at extremely dry levels were 200—720 cm and 360—720 cm, respectively. The soil water storage in the depth range of 0—1 000 cm was 987.55 mm and 977.78 mm and less than that of rainfed farmland with similar topography.（2） The roots of Medicago sativa L. and Caragana korshinskii mainly concentrated in the 0—120 cm soil layer, which accounted for 45.66% and 57.54% of the total root dry weight in the 0—1 000 cm profile, and the root length density accounted for 44.45% and 67.58%.（3） The distribution of N and P nutrients in the soil of Medicago sativa L. and Caragana korshinskii lands was consistent with that of root system. The average total N content in the surface layer of 0—120 cm was 0.53 g/kg and 0.58 g/kg, respectively, which was 1.77 times and 1.87 times of the average total N content in the soil of 0—1 000 cm. The average total P content in the range of 0—120 cm was 0.51 g/kg and 0.53 g/kg, which was similar to the average total P content in the 0—1 000 cm soil layer. [Conclusion] The characteristics of soil moisture, root system and nutrient of artificial forest and grassland under the background of deep soil drying in the loess hilly area are special. In-depth study on the synergistic relationship of soil moisture, root system and nutrient in deep soil of artificial forest and grassland is helpful for the sustainable development of regional artificial vegetation and the regulation of soil carbon cycle. [ABSTRACT FROM AUTHOR]

Copyright of Ordnance Industry Automation is the property of Editorial Board for Ordnance Industry Automation and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Urban Architecture & Development is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To probe into the effect of bisoprolol on H9C2 cardiomyocyte injury induced by ischemia reperfusion (I/ R) and its mechanism. METHODS: H9C2 cardiomyocytes were cultured in vitro and divided into the control group ( Control group ), hypoxia / reoxygenation ( H / R) model group ( Model group ), bisoprolol group (Bisoprolol group), bisoprolol + apoptosis signal-regulated kinase 1 (ASK1) recombinant protein group (Bisoprolol+ ASK1 group). Except for the Control group, the H9C2 cardiomyocytes were treated with H / R injury in the rest groups. The cell viability was detected by cell counting kit (CCK8) method; the cell apoptosis was detected by flow cytometry; the myocardial injury markers [ lactate dehydrogenase ( LDH ), cardiac troponin T ( cTnT )] and inflammatory factors [ interleukin 6 ( IL-6), interleukin 1β ( IL-1β) and tumor necrosis factor α (TNF-α)] levels were measured by enzyme-linked immunosorbent assay; the calcium ion concentration in cells was measured by laser confocal assay; the ASK1-c-Jun amino-terminal kinase ( JNK) / p38 mitogen-activated protein kinase ( p38 MAPK) signaling pathway-related protein expression was detected by Western blotting. RESULTS: Compared with the Control group, the H9C2 cell viability, LDH and cTnT levels were lower, the cell apoptosis rate, IL-6, IL-1β and TNF-α levels were higher, the calcium ion concentration, p-ASK1 / ASK1, p-JNK/ JNK and p-p38 MAPK/ p38 MAPK ratio were higher in the Model group, with statistically significant differences (P<0. 05). Compared with the Model group, the H9C2 cell viability, LDH and cTnT levels were higher, the cell apoptosis rate, IL-6, IL-1β and TNF-α levels were lower, the calcium ion concentration, p-ASK1 / ASK1, p-JNK/ JNK and p-p38 MAPK/ p38 MAPK ratio were lower in the Bisoprolol group and Bisoprolol + ASK1 group, with statistically significant differences ( P < 0. 05). Compared with the Bisoprolol group, the H9C2 cell viability, LDH and cTnT levels were lower, the cell apoptosis rate, IL-6, IL-1β and TNF-α levels were higher, the calcium ion concentration, p-ASK1 / ASK1, p-JNK/ JNK and p-p38 MAPK/ p38 MAPK ratio were higher in the Bisoprolol +ASK1 group, with statistically significant differences (P<0. 05). CONCLUSIONS: Bisoprolol can improve H9C2 cardiomyocyte injury induced by I/ R through inhibiting inflammatory response and calcium overload, its mechanism may related to the inhibition of ASK1-JNK/ p38 MAPK signaling pathway activation. [ABSTRACT FROM AUTHOR]

Copyright of Urban Architecture & Development is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Ordnance Industry Automation is the property of Editorial Board for Ordnance Industry Automation and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Beijing University of Technology is the property of Journal of Beijing University of Technology, Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Rural Water & Hydropower is the property of China Rural Water & Hydropower Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

α-Hydroxy-3,6-diene compounds can be synthesized by the nickel-catalyzed carboalkenylation of 1,3-diene with aldehydes and alkenylzirconium reagents. 1,4-Diene structure of α-hydroxy-3,6-diene compounds is a common structural unit in a variety of bioactive molecules, which has been widely used in natural products and can be transformed into diverse polyene structures, and also can be used as a key intermediate for organic synthesis. Mass spectrometric characterization of these α-hydroxy-3,6-diene compounds presents unique ion of ［M-17]+. A series of α-hydroxy-3,6-diene compounds, including aroma-α-hydroxy-3,6-diene compounds (Ⅰ) and aliphatic α-hydroxy-3,6-diene compounds (Ⅱ), showed special ［M-17]+ during the measurement by atmospheric pressure chemical ionization time-of-flight mass spectrometry (APCI-TOF MS), which might be the fact that these compounds contained the hydroxyl groups and the dehydroxylation process were affected by the unsaturated bond. The results showed that aroma-α-hydroxy-3,6-diene compounds could directly lose one hydroxyl to form ［M-OH]+ ions due to the conjugated π bond, while aliphatic α-hydroxy-3,6-diene compounds formed the dehydration and protonation ion ［M-H2O+H]+. It was notably that aroma-α-hydroxy-3,6-diene compounds tended to form the dehydration and protonation ions if they could form conjugated structure by adding protons after dehydration. It was difficult to determine the correct fragmentation pathway of compounds only by simple inference of mass spectrometry. Combined with density functional theory (DFT) calculations, positive ion mode of high resolution mass spectrometry and tandem mass spectrometry was adopted to demonstrate the possible existence of Ⅰ and Ⅱ processes of the nine compounds. It was found that the prediction of a few compound structures did not agree with the actual pyrolysis process. The aforesaid results indicated that gas phase ions in mass spectrometry tended to generate stable structures with low electronic and thermal energies, and the position of unsaturated bonds affected the dehydroxylation process. Simple collision induced dissociation (CID) pyrolysis derivation was applicable for most α-hydroxy-3,6-diene compounds, but ionization process of some special compounds was unreasonable and needed theoretical evidence. Therefore, DFT calculation results was used to support the mass spectrometric process. These results are helpful for accurate identification of the structures of α-hydroxy-3,6-diene compounds or similar compounds, and provide theoretical basis and guidance for the ion writing specification in MS spectra and the fragmentation pathways of these compounds. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Stem cells have been the focus of regenerative medicine research. Many factors affect the biological characteristics of stem cells, among which donor age is one of the critical factors. OBJECTIVE: To observe biological characteristics of two types of inbreed line miniature pigs of Wuzhishan adipose-derived mesenchymal stem cells so as to screen the optimal seed cell source. METHODS: Inbreed line miniature pigs of Wuzhishan adipose-derived mesenchymal stem cells aged 4 and 12 months were selected as materials. The passage 3 and passage 10 of cells were selected to observe their cell morphology, draw cell proliferation curve, and detect cell surface antigen, cell cycle and apoptosis. Afterwards, osteogenic differentiation was performed with Alizarin red staining, followed by adipogenic differentiation with Oil Red O staining and cell senescence β-galactosidase staining. RESULTS AND CONCLUSION: (1) The morphology of cells at 4 and 12 months both showed vortex-shaped adherent growth. The expression of surface markers CD29, CD44 and CD90 was positive, while that of CD8a, CD45 and HLA-DR was negative. (2) The proliferation of cells at 4 and 12 months was stronger in passage 3. In passage 10, the percentage of cells in the G0/G1 phase (P < 0.001) and early apoptosis (P < 0.001) increased, while in the S and G2/M phases, it decreased at 12 months compared with 4 months (P < 0.001). (3) There was no significant difference in the differentiation ability of cells at passage 3 in vitro between 4 and 12 months. The calcification nodule rate and lipid droplet rate of cells at 12 months of passage 10 were significantly lower than at 4 months of passage 10 (P < 0.001). (4) The β-galactosidase of cells was almost not expressed at 4 and 12 months of passage 3. However, in the passage 10, the positive rate of cells at 12 months was significantly higher than that at 4 months (P < 0.001); and the cell morphology became more flattened, hypertrophic and less refractive. (5) It is concluded that adipose-derived mesenchymal stem cells of inbreed line miniature pigs of Wuzhishan at 4 months showed stronger proliferation and differentiation ability, while showed lower senescence on β-galactosidase activity, suggesting that 4-month cells are more suitable for autograft. [ABSTRACT FROM AUTHOR]

Objective To screen out the differentially expressed long non-coding RNA（LncRNA）in the glioma tis⁃ sues and to verify the results. Methods We collected 19 cases of glioma tissues and 5 cases of normal brain tissues and conducted the genome high-throughput sequencing. EdgeR software was used to analyze the expression profiles of lncRNA， and the differentially expressed lncRNAs were screened out based on fold change and P-value. In addition，70 cases of glioma tissues and 20 cases of normal brain tissues were collected，and qPCR was used to verify the first 3 up-regulated and down-regulated lncRNAs in the glioma tissues. Results There were 3 040 differentially expressed lncRNAs in the glioma tissues，among which，1 233 up-regulated and 1 807 down-regulated lncRNAs were detected. TCONS_00010961， ENST00000443546. 1，and ENSG00000262119 were the first 3 up-regulated lncRNAs whose expression was higher than that of the normal brain tissues； and ENST00000531508. 1，TCONS_00004034，and ENST00000504876. 1 were the first 3 down-regulated lncRNAs whose expression was lower than that of the normal brain tissues，and the outcomes were consistent with the sequencing results. Conclusions The differentially expressed lncRNAs are screened out in the glioma tissues. This founding lays the foundation for further studies，and provides theoretical basis for the clinical diagnosis，treatment and prognosis of glioma. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Applied Ecology / Yingyong Shengtai Xuebao is the property of Chinese Journal of Applied Ecology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Research of Environmental Sciences is the property of Research of Environmental Sciences Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal on Communication / Tongxin Xuebao is the property of Journal on Communications Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Research & Exploration in Laboratory is the property of Research & Exploration in Laboratory and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)