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目的探讨过氧化物酶（POD）法检测新生儿血清游离胆红素（UB）的临床适用性及应用价值。方法向胆红素标准品溶液（1、2、3 μmol/L）加入H2O2（0.33 mol/L）和三种不同终末浓度（0.019、0.038、0.075 μg/mL）的辣根过氧化物酶（HRP）以获得标准化的HRP一级速率常数Kp；新生儿血清上样量25 μL，稀释倍数41.6，于37 ℃且避光条件下用2.4、4.8 μg/mL HRP检测血清UB水平，并验证该方法学的准确性、精密度以及稳定性；收集33例黄疸患儿的一般情况及临床资料，主要包括血清总胆红素（TSB）、间接胆红素（ IDB）、白蛋白（ALB）、胆红素与白蛋白摩尔比值（BAMR）等；用Graphpad Prism 8.0统计分析实验数据。结果在pH值为 7.4±0.2，反应温度37 ℃且避光条件下，标准化Kp为（7.20±1.08） mL·μg-1·min-1。HRP活性和UB水平分别于-20 ℃冻存3周和1周内无明显下降。不同UB水平血清样品的平均日内、日间变异系数均小于10%。本研究用POD法检测33例黄疸患儿（胎龄≥35周）的血清UB范围为（0.32～1.20） μg/dL，与TSB、IDB及BAMR均显著相关。有5例UB水平≥1.0 μg/dL，强光疗者占60%（3/5）。结论采用POD法结合常见设备分光光度计检测新生儿血清UB水平不仅操作简易、检测快速，且试剂成本低，具有良好的准确性和精密度，便于临床开展。血清UB的测定对新生儿黄疸的临床管理有一定的指导意义。

目的探讨体表光学图像引导技术（SGRT）联合图像引导放疗技术（IGRT）在乳腺癌术后放疗中实施等中心双引导复位的可行性，评估新的复位模式相对于传统复位模式的误差精度差异。方法回顾性分析2021年7月13日至2022年10月15日在中山大学肿瘤防治中心ELEKTA infinity加速器放疗的乳腺癌保乳术后患者，按不同复位方式分为模拟机复位组（模拟组）41例和双引导复位组（双引导组）40例。模拟组采用模拟机进行复位，首次用CBCT扫描并记录摆位误差；双引导组采用体表光学设备AlignRT引导摆位并联合CBCT进行等中心双引导复位，记录CBCT配准获取的摆位误差。分别计算并比较两种复位方式的胸壁靶区（CROI）的整体摆位误差、患侧锁骨头区域（SROI）的局部残余误差和复位时间，分析SGRT联合IGRT双引导复位的CBCT误差值分布的优点。结果模拟组和双引导组在CROI区域的整体摆位误差（X/cm、Y/cm、Z/cm、Rx°、Ry°、Rz°）中，除了Ry、Rz方向无统计学意义（P>0.05）外，其他方向摆位误差具有统计学意义（PP>0.05）外，其他方向均具有统计学意义（Pt=-24.555，P=0.000，差异具有统计学意义（P

目的探讨早产儿脑白质损伤（WMD）的围产期高危因素及血细胞参数的相关性，为早期发现并规避早产儿WMD的发生提供科学依据。方法采用回顾性病例对照研究设计，收集南方医科大学附属深圳市妇幼保健院新生儿科2018年1月1日至 2020 年12月31日经核磁共振检查诊断为WMD的早产儿85例为病例组，并以胎龄为匹配条件选取同期115例经核磁共振检查未诊断WMD患者作为对照组。采用SPSS22.0统计学软件进行围产期相关因素的单因素分析、血细胞参数单因素分析、构建多因素Logistic回归模型分析WMD的相关因素，并对其中有意义的连续性变量进行接受者操作特性曲线分析，以获得WMD危险因素截断值，最后进行有序Logistic回归分析WMD严重程度的影响因素。结果①围产期相关因素单因素分析显示，两组在孕期贫血率、产前足疗程激素使用率、早发败血症发生率、机械通气（≥7 d）比例、低血压发生率、早产儿脑室周围-脑室内出血（PIVH）Ⅲ-Ⅳ级及有血流动力学意义的动脉导管未闭（hsPDA）发生率之间的差异均有统计学意义（均P P 9/L。⑤有序Logistic回归分析，结果显示：早发型败血症是WMD严重程度的危险因素，而胎龄和血小板分布宽度则是其保护因素及血细胞学指标。结论早产儿机械通气（≥7 d）、生后1周血细胞MCH和PLT减低可能是预测早产儿WMD发生的独立危险因素及相关血细胞学指标，而产前应用足疗程的糖皮质激素则是其保护因素；早发型败血症及越小的胎龄及PDW可能是WMD严重程度的危险因素。

在大规模可再生能源渗透的背景下，利用储能装置促进可再生能源消纳并降低用户成本逐渐成为发展趋势。以电能用户为研究对象，深入挖掘并构建用户侧储能综合效益模型；建立多用户共享储能的容量配置投资决策模型，基于用电形态相似度，提出了以高、低2种相似度为标准的共享储能模式下用户筛选方式，以提高共享储能的经济效益。算例分析对比了高、低2种用电形态相似度下用户集体共享储能的经济性，给出了共享储能容量配置的投资决策结果，以高用电形态相似度作为用户筛选标准可以获得更大的共享储能经济效益，并且经济效益与用户数量呈现正相关。

： 【目的】研究α2-巨球蛋白（α2M）对X线照射后人皮肤成纤维细胞（HSF）超氧阴离子（.O 2 - ）含量、超 氧化物歧化酶（SOD）活性和HSF细胞向肌成纤维细胞转化过程中的影响。【方法】分别使用0、5、10、15、20 Gy X 线照射HSF细胞，在照射后第1天检测细胞培养基上清液中。O 2 - 含量、SOD活性的变化，在照射后第5天采用 Western blot检测α-平滑肌肌动蛋白（α-SMA）的蛋白表达变化，以选定合适的照射剂量。用以上合适剂量照射 HSF细胞，分别于照射前1 h、照射后1 h实验组细胞培养基中加入终浓度为0.25 mg/mL，0.5 mg/mL的α2M，检测 并比较不同实验组细胞培养基上清液中.O 2 - 含量、SOD活性和α-SMA蛋白表达的变化情况。【结果】5~20 Gy X线 照射 HSF 细胞后，.O 2 - 含量逐渐增多，SOD 活性逐渐降低，α-SMA 蛋白表达量逐渐升高，差异具有统计学意义 （P

为了解决AC（接入控制器）基于多核快转软件平台下控制面和数据面数据的快速转发,文章基于该多核快转软件平台深入分析了无线网络AC控制面和数据面实现邻居表信息同步的原因,介绍了控制面和数据面邻居表信息同步的原理,提出了一种适合无线网络AC的控制面和数据面邻居表信息同步的方案,并编程实现。实验结果表明,该方案能够实现无线AC控制面和数据面邻居表信息的同步。

随着基于以太网的无源光网络（EPON）商用规模的逐步扩大,不同厂商设备间的互通测试以及EPON系统的工程验收测试和运行维护变得日益迫切,为此我们设计开发了EPON测试仪。文章分析了EPON测试仪的功能需求,简述了EPON测试仪的总体框架,着重阐述了EPON测试仪硬件平台的设计及其核心功能的现场可编程门阵列（FPGA）的实现。

MS EPON为用户提供电信运营级服务质量的电路业务和以太网数据业务的多业务宽带接入.作为电信级传输接入系统,MS EPON的传输性能在线监测是必不可少的.文章应用比特间插奇偶校验原理,提出了一种利用以太网帧前导码的一个字节作为BIP校验以实现对MS EPON系统传输性能在线监测的方法,并对监测性能进行了分析,证明了方案的可行性.

ONU的注册是EPON系统正常运行的前提.在系统运行中新ONU自动加入或故障ONU重新加入而不影响正常运行的ONU是PON系统可扩展性好、操作维护方便的重要体现.文章分析了对ONU自动加入的基本需求,详细描述了ONU自动加入过程,并对冲突处理方法进行了分析比较.

利用业务调度算法实现不同业务等级间的动态带宽分配 （ DBA） ,从而保证接入业务的服务质量 （ Qo S） ,是异步转移模式无源光网络 （ APON）系统的关键技术之一 .文章所设计的APON系统业务调度算法 ,通过基本带宽分配和剩余带宽再分配实现了业务等级间的动态带宽分配 ,算法建模仿真和系统实际测试证明算法具有良好的性能 ,实现简单 ,可用性强

在采用 TDMA协议的无源光网络中 ,突发同步是一项关键技术 ,一般采用基于多相时钟的关键字检测法。本文提出了一种新颖的基于数据延时的突发同步技术 ,采用了高速 FP-GA和 ECL有源延时器的方式实现 1 55Mbit/s突发同步器。由于采用同一主时钟对数据抽样 ,不需要进行相位校准 ,简化了电路的设计 ;相对于传统的 ASIC设计方案 ,该方案具有开发成本低、开发周期短的优点

由于用户对宽带业务的需求逐步增长 ,传统的模拟接入网正在向数字化的高速宽带网络过渡 .其中基于 ATM的无源光网络 （APON）又是接入网研究中的热点 .本文先介绍APON的基本概念和特点 ,然后在此基础上对 APON系统应采取的保护倒换方式进行分析 ,并给出了几种保护倒换结构的配置形式

以太网无源光网络（EPON）已成为应用最广的宽带接入技术之一,为其配备强大的网管系统是十分必要的。文章设计了EPON网管资源管理子系统,以实现对网络资源的管理和状态的实时监控。首先介绍了EPON网管系统的总体架构,随后重点阐述了资源管理子系统的设计方案,最后分别在网管客户端、服务器端和代理侧予以实现。

文章主要对一种基于微波接入全球互通（WiMAX）的宽带无线接入网的射频光纤传输系统的方案进行了研究。该方案主要描述了系统的中心站与基站间的下行链路的结构。对其功率预算和载波带宽选择问题进行了理论推导和分析。其分析结果对该系统在功率以及载波带宽选择方面具有一定的指导意义。

文章提出一种适用于千兆以太网无源光网络（EPON）的支持多业务的动态带宽分配（DBA）算法,该算法采用单阈值整帧上报和阈值动态调整解决了EPON中存在的时隙碎片和队头阻塞问题。同时,文章对算法中的关键参数——设定周期的大小与算法性能的关系进行了深入的研究分析,以指导算法设定周期的确定。

文章介绍了E1 over基于以太网的无源光网络（EPON）的关键技术中时钟同步的实现方法,通过对自适应法的研究和改进,优化了时钟输出抖动的指标,有效地保障了EPON系统的服务质量（QoS）指标。相对于时间标签法,自适应法在分组网络中进行时钟同步方面具有更广泛的适用空间。

文章从产生偏振模色散 （ PMD）的机理出发 ,对单模光纤中 PMD的动力学方程及其几何模型进行了研究

【目的】构建反义c-myb 重组逆转录病毒载体并建立其包装细胞株。【方法】采用RT-PCR 方法, 获取目的基因, 通过TA 克隆方法克隆入pUC19, 再反向亚克隆入逆转录病毒载体pDOR 中。采用DOTAP 法将重组逆转录病毒载体转入包 装细胞, 以NIH3T3 细胞为靶细胞测定产病毒滴度。【结果】序列测定结果与GenBank 中序列一致。c-myb 基因片段已定向 克隆入pDOR。细胞转染表明, 已形成稳定的包装细胞株PA317/ pDOR-my b, 产病毒滴度达5.2 ×104 ～ 9.5 ×104 CFU/m L。 【结论】成功构建了含有反义c-my b 的重组逆转录病毒质粒, 并建立其包装细胞株。

【目的】为探索腺病毒载体在基因治疗中的应用及其表达目的抗原的有效性。【方法】用 BamHⅠ 、EcoRⅠ双切 质粒 pBluscript KS+ -HBs, 回收 S 基因片段, 定向克隆插入质粒 pACCMVpL 中相应的酶切位点, 通过快速抽提酶切鉴定筛选 重组质粒。【结果】挑取的 8 个克隆经 BamHⅠ 、EcoRⅠ酶切鉴定, 有 950 bp 的目的基因片段, 证明为重组体。【结论】成功 构建表达 HBsAg 基因的重组腺病毒载体。

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Objective To investigate the expression, distribution, and activity of thyroid-stimulating hormone receptor (TSHR) in human microglia (HMC3). Methods In this study, we used human microglial cells (HMC3) as the experimental group and human thyroid cells and hepatocytes as the controls. Reverse transcription polymerase chain reaction was used to detect the expression of TSHR mRNA to identify the transcript of TSHR mRNA. Western blotting and immunofluorescence staining analysis was used to detect TSHR protein level and the distribution in HMC3 cells. HMC3 cells were divided into the 0, 20, 50, 100, 200 ng/mL thyroid-stimulating hormone receptor-stimulating antibody (TSAb) groups and negative control group. Each group was preincubated with 100 µmol/L phosphodiesterase inhibitor (IBMX) for 60 minutes. The corresponding concentrations of TSAb were added to cells of the TSAb groups and then they were incubated for 24 h. We measured cyclic adenosine monophosphate (cAMP) levels by immu noassay kits to verify the functional activity of TSHR in HMC3 cells. Results TSHR mRNA was detected in HMC3 cells with the same sequence as mRNAs derived from human thyroid cells and hepatocytes. TSHR protein was expressed in the cell membrane of HMC3 cells. Compared with the 0 ng/mL TSAb group, there was no significant statistical difference in cAMP levels in the 20 ng/mL TSAb group. However, cAMP levels increased in the 50, 100, and 200 ng/mL TSAb groups, and the high-concentration group had higher cAMP levels than the low-concentration group (P<0.05 or P<0.01). Conclusion TSHR is expressed in the cell membrane of human microglia and has a signaling function. [ABSTRACT FROM AUTHOR]

Copyright of Current Biotechnology (2095-2341) is the property of Chinese Academy of Agricultural Sciences, Institute of Biotechnology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Andrology is the property of Chinese Journal of Andrology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Pulp & Paper is the property of China Pulp & Paper Magazines Publisher and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)