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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the osteogenic differentiation by adipose-derived stem cells(ADSCs) induced by puerarin in rats with osteoporosis after ovariectomy. Methods ADSCs were isolated, cultured, passaged, and identified from postmenopausal osteoporosis rats. The maximum effective concentration of puerarin to induce ADSCs in postmenopausal osteoporosis rats was determined. Results The osteogenic differentiation of ADSCs induced by puerarin in postmenopausal osteoporosis rats was positively correlated with the time and concentration. When the concentration was 12 mmol/L, the most significant osteogenic differentiation promoting effect was observed with non-toxic reaction. Conclusion Puerarin strongly induces the osteogenic differentiation of ADSCs in postmenopausal osteoporosis rats. [ABSTRACT FROM AUTHOR]

BACKGROUND: Treatment strategy for vertebral burst fracture remains controversial because of its complex morphological changes. There is a lack of knowledge concerning the fixation of thoracolumbar burst fracture (A3.3 by AO classification). OBJECTIVE: To investigate the biomechanical characteristics of thoracolumbar burst fracture (A3.3 by AO classification) after screw fixation, thereby providing reference for choosing an appropriate placement method. METHODS: (1) Three-dimensional motion test: the samples were divided into complete set (group A), L1 burst fracture (A3.3 by AO classification) (group B), trans-vertebral fixation (group C), screw fixation at the unilateral fracture level (group D), screw fixation at the bilateral fracture levels (group E), and screw fixation at the bilateral upper fracture levels (F). Several swine spinal specimens served as group A, L1 was modeled into A3.3 by AO classification fracture after three-dimensional motion. (2) The compressive stiffness test: one fresh specimen served as complete group (group A), B, C, D, E, and F groups underwent compressive stiffness test. RESULTS AND CONCLUSION: (1) Three-dimensional motion tests: the range of motion in the group B was significantly decreased after C, D, E, and F fixations (P < 0.05). Compared with groups C, D and E, the range of motion at the directions of anteflexion, left flexion, right flexion, left and right rotation in the group F was significantly increased in the group F (P < 0.05); the range of motion in the groups E and D was significantly smaller than that in the groups C and F (P < 0.05); there was no significant difference between groups D and E (P > 0.05). (2) The stiffness in the other groups was significantly higher than that in the group B, especially group E, and the groups D and A did not differ significantly. (3) These results indicate that the fixation at the unilateral fracture level and bilateral fracture levels both can significantly improve the stability of thoracolumbar burst fracture (A3.3 by AO classification), and the former can reduce the economical burden and placement complications. [ABSTRACT FROM AUTHOR]