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【目的】探讨去琥珀酰化酶Sirtuin5（SIRT5）对受体相互作用蛋白140（RIP140）诱导的心肌线粒体能量代谢紊乱的影响。【方法】利用腺病毒感染心肌细胞诱导 RIP140 过表达，利用质粒转染诱导 SIRT5 基因过表达，siRNA 干扰敲低 RIP140、SIRT5表达；qRT-PCR和Western blot检测SIRT5、RIP140的表达变化；qRT-PCR检测线粒体编码基因的表达情况， TMRE染色法评估线粒体膜电位，试剂盒检测细胞耗氧和ATP生成情况。所有数据均来自至少3次的独立实验。【结果】过表达RIP140明显抑制SIRT5的表达（P

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Objective To learn the clinical characteristics of concurrent acute-on-chronic liver failure (ACLF) and hepatorenal syndrome (HRS), and to investigate the predictive factors for HRS in patients with ACLF. Methods A total of 806 patients with ACLF who were admitted to our hospital from January 2012 to May 2014 were selected and divided into two groups according to the incidence of concurrent HRS. Clinical indices and laboratory test results were analyzed in the two groups, and the multivariate logistic regression analysis was used to figure out independent indices for the prediction of HRS in patients with ACLF. A prediction model was established and the receiver operating characteristic curve was drawn to evaluate the accuracy of the prediction model. Comparison of continuous data between the two groups was made by t test, and comparison of categorical data between the two groups was made by χ² test. Results In all patients with ACLF, 229 had HRS and 577 had no HRS. The univariate logistic regression analysis showed that hepatic encephalopathy, peritonitis, infection, age, cystatin C (Cys-C), serum creatinine (SCr), blood urea nitrogen, albumin, prealbumin, total bilirubin, direct bilirubin, total cholesterol, K+, Na+, phosphorus, Ca2+, prothrombin time, prothrombin activity, international normalized ratio, and hematocrit were significant predictive factors for HRS. The multivariate logistic regression analysis showed that concurrent peritonitis, Cys-C, SCr, and HCO3- were independent predictive factors for HRS in patients with ACLF (OR=3.155, P＜0.01; OR=30.773, P＜0.01; OR=1062, P＜0.01; OR=0.915, P＜0.05). The model was proved of great value in prediction. Conclusion Concurrent peritonitis, Cys-C, SCr, and HCO3- are effective predictive factors for HRS in patients with ACLF. [ABSTRACT FROM AUTHOR]