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Objective: To investigate the influences of astragalus polysacharide(APS) on anti-tumor effect and immune function of esophageal carcinoma(EPC) rats through phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) signal pathway. Methods: 50 healthy 6-week-old SPF grade SD rats were randomly divided into model group(group M), cisplatin group(group S), APS low dosage group(group APSL), APS middle dosage group(group APSM) and APS high dosage group(group APSH), with 10 rats in each group. EPC model rats were established by transplantation of Eca109 cells, and 3mg/kg cisplatin and 100, 200, 400 mg/kg APS were given to each rat. Tumor volume and tumor mass were detected, tumor growth inhibition rate, thymus index and spleen index were calculated, histomorphology was observed by HE staining, CD3+, CD4+, CD8+T lymphocyte population were detected by flow cytometer, the relative expressions of PI3K and Akt protein were detected by Western blot. Results: After intervention, tumor volume and tumor mass of group S and group APS were all lower than group M(P＜0.05); the tumor volume and tumor mass of group S and group APSH were all lower than group APSM and group APSL(P＜0.05); the tumor growth inhibition rate of each APS group were 45.59%, 32.35% and 17.65%. The thymus index and spleen index of group S were lower than group M(P＜0.05); the thymus index and spleen index of each APS group were all higher than group S(P＜0.05); while group APSH and APSM were higher than group M(P＜0.05). The CD3+, CD4+ and CD4+/CD8+ of each APS group were higher than group S and group M, while the CD8+ was lower(P＜0.05); group APSH, APSM and APSL also had statistical differences(P＜0.05). The relative expressions of PI3K/Akt signal pathway protein of group S and each APS group were lower than group M(P＜0.05); group APSH, APSM and APSL also had statistical differences(P＜0.05). Conclusion: APS exerts inhibition effect on EPC via regulation of PI3K/Akt signal pathway and immune function, which can provide new thought and theoretical basis for clinical treatment of EPC. [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore and analyze the correlation between serum IL-8 gene polymorphism and ESCC after radical resection. Methods:From August 2017 to June 2020, 98 cases of patients with esophageal squamous cell carcinoma diagnosed and treated in our hospital were selected as the research object, and the serum IL-8 expression level were detected. All patients were treated with radical surgery, and the prognosis of the patients were followed up and given correlation analysis. Results: All patients were followed up to July 2021. The average follow-up time were 25.69± 2.58 months. There were 28 deaths and the mortality rate were 28.6 % (death group). The expression level of serum IL-8 in the death group were higher than that in the survival group (P<0.05). The genotype frequencies of all patients were conformed to the Hardy-Weinberg equilibrium rule, which confirmed that the samples were representative of the population. The AA genotype of the IL-8 gene promoter rs4073A/T were higher than that of the death group(P<0.05), and the TT genotype were lower than that of the death group (P<0.05). There were also statistical differences in the frequency distribution of A and T alleles compared between the two groups (P<0.05). Linear correlation analysis showed that the AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 were correlated with prognosis and mortality(P<0.05). Multivariate logistic regression analysis showed that the AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 were the main factors leaded to followed-up death (OR=2.051, 3.094, P<0.05). Conclusions: Patients with esophageal squamous cell carcinoma still have certain mortality rate after radical resection. There are correlation between the death of patients and the polymorphism of serum IL-8 gene. It are also often accompanied by high expression of IL-8. The AA genotype and A allele of IL-8 gene promoter rs4073A/T, expression level of serum IL-8 are The main factor lead to the death of patients. [ABSTRACT FROM AUTHOR]

Copyright of Silicone Material is the property of Silicone Material Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To explore and study the effect and mechanism of artesunate on angiogenesis in nude mice with lung can-cer. 27 cases of nude mice were randomly divided into 3 groups-lung cancer group, artesunate 1 group and artesunate 2 groups,with 9 cases in each group. All mice were given intraperitoneal injection of lung cancer A549 cells for tumorigenesis, After successfullytumorigenesis, the lung cancer group, artesunate group 1 and artesunate group 2 were given phosphate buffer, 2.0 mg/mL and 4.0 mg/mLartesunate for intragastric administration, once a day, lasted 10 d. All cases were successfully tumorigenic. The weight of trans-planted tumors in artesunate 1 group and artesunate 2 group were lower than that of the lung cancer group (P<0.05), and the tumor inhibition rate were higher than that of the lung cancer group (P<0.05), there were statistically significant difference compared between arte-sunate group 2 and artesunate group 1 (P<0.05). The apoptosis index of transplanted tumor cells in artesunate group 2 and artesunategroup 1 were higher than that of lung cancer group (P<0.05), and artesunate group 2 were higher than artesunate group 1 (P<0.05). Thedensity of lymphatic vessels around transplanted tumor tissues in artesunate 2 group and artesunate 1 group were lower than that of lungcancer group (P<0.05), and artesunate 2 group were lower than artesunate 1 group (P<0.05). The levels of serum tumor necrosis factor(TNF)-α and interleukin(IL)-6 in artesunate group 2 and artesunate group 1 were lower than those in lung cancer group(P<0.05), the arte-sunate group 2 were lower than the artesunate 1 group(P<0.05). The relative expression levels of connective tissue growth factor (CTGF)and vascular endothelial growth factor (VEGF) proteins in transplanted tumors in artesunate group 2 and artesunate group 1 were lowerthan those in the lung cancer group(P<0.05), the artesunate group 2 were lower than the artesunate group 1 (P<0.05). The application of artesunate in nude mice with lung cancer can inhibit the expression of CTGF and VEGF protein, reduce the expression of inflammatory factors, and promote tumor cell apoptosis, thereby inhibit tumor proliferation and angiogenesis. [ABSTRACT FROM AUTHOR]

Copyright of Silicone Material is the property of Silicone Material Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)