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Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Computer Engineering & Applications is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the changes of MAPK and NF-κB pathways after co-culture of intestinal epithelial cells and macrophages in micro-inflammatory environment，and to explore the inter-cell communication mechanism of innate immune system to maintain intestinal immune balance. Methods Human colon adenocarcinoma epithelial cell lines Caco2 and HCA7 were cultured with human leukemia mononuclear macrophage cell line THP1 in Transwell chamber，respectively. After stimulation of tumor necrosis factor-α（TNF-α，5 ng/mL）for different time，the MAPK pathway related proteins including phosphorylated extracellular signal-regulated kinase（p-ERK）and phosphorylated mitogen-activated protein kinase（p-p38），and NF- κB pathway-related proteins including phosphorylated NF- κB inhibitor- α（pIκBα），and phosphorylated nucleoprotein（p-p65）were detected by Western blotting. THP1 cells in the logarithmic growth phase were divided into the CaCo2 medium group and DMEM medium group. After centrifugation，they were suspended in CaCo2 conditioned medium and DMEM complete medium，respectively. After adding TNF-α 5 ng/mL for different time，the protein expression levels of p-ERK，p-p38，p-IκBα and p-p65 were detected by Western blotting. The mRNA expression levels of TNF-α-related cytokines［interleukin（IL）-8，IL-6，and IL-1β］and chemotactic cytokines CCL2 and CCL3 of CC subfamily were detected by RT-PCR. Results In the mono-cultured cells，the expression levels of p-ERK，p-p38 and p-p65 in CaCo2 cells increased after TNF-α stimulation for 30 min，and the expression levels of p-p38，p-IκBα，and p-p65 in THP1 cells increased after TNF-α stimulation for 90 min（all P<0. 05）. Compared with the mono-cultured cells with the same time of TNF-α stimulation，the expression levels of p-ERK，p-p38 and p-IκBα in CaCo2 cells after co-culture decreased after TNF-α stimulation for 90 min（all P<0. 05）；the expression levels of p-ERK， p-p38，p-IκBα and p-p65 in the THP1 cells after co-culture decreased after 90 min of TNF-α stimulation（all P<0. 05）. In the mono-cultured cells，the expression levels of p-ERK，p-p38，p-IκBα and p-p65 in HCA7 cells increased after 120 min of TNF-α stimulation，and the expression levels of p-ERK，p-p38 and p-p65 in THP1 cells increased after 30 and 120 min TNF-α stimulation（all P<0. 05）. Compared with the mono-cultured cells with the same time of TNF-α stimulation，the expression levels of p-ERK，p-IκBα and p-p65 in HCA7 cells after co-culture decreased after TNF- α stimulation for 120 min，and the expression levels of p-ERK，p-p38 and p-p65 in the co-cultured ThP1 cells decreased after TNF-α stimulation for 30 and 120 min（all P<0. 05）. Compared with DMEM medium group with the same time of TNF-α stimulation， the expression levels of p-ERK，p-p38，p-IκBα and p-p65 decreased after 120 min of TNF-α stimulation in CaCO2 conditioned medium group. Meanwhile，the mRNA relative expression levels of IL-8，IL-1β and CCl2 decreased after TNF-α stimulation for 60 min（all P<0. 05）. However，the relative expression levels of IL-6 and CCL3 mRNA did not significantly change between these two groups（all P>0. 05）. Conclusions The activation of MAPK and NF-κB pathways and the secretion of pro-inflammatory factors were inhibited after co-culture of intestinal epithelial cells CaCO2，HCA7 and macro-phages THP1 in a micro-inflammatory environment. The signal communication between these cells was mediated by the secretion of soluble mediators. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)