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Copyright of Electric Machines & Control / Dianji Yu Kongzhi Xuebao is the property of Electric Machines & Control and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Applied Mathematics & Mechanics (1000-0887) is the property of Applied Mathematics & Mechanics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To investigate the regulatory effect of miR-1298 on hypoxic-ischemic nerve injury by cell culture in vitro. Firstly, the modeling effect of oxygen glucose deprivation/reoxygenation (OGD/R) of rat PC-12 cells was determined by cell activity detection and lactate dehydrogenase (LDH) cytotoxicity. At the same time, the expression difference of miR-1298 was detected by real-time fluorescence quantitative PCR (RT-qPCR). The miR-1298mimic, mimic NC, miR-1298 inhibitor and inhibitor NC were transfected into rat PC-12 cell line in vitro. The transfection efficiency of miR, mimic NC, inhibitor and inhibitor NC was detected. After OGD/R treatment, the cells were divided into control group, OGD/R group, mimicgroup, mimicNC group, inhibitor group and inhibitor NC group. The apoptosis of PC-12 cells in each group was detected by flow cytometry, and the expression of PC-12 apoptosis related protein B lymphoma-2 gene (BCL-2) and Bcl-2 associated X protein (BAX) were detected by Western blot. After OGD/R treatment, the survival rate of PC12 cells decreased significantly compared with the control group, and the leakage rate of LDH increased significantly (all P<0.05). The relative expression of miR-1298 in model cells was significantly lower than that in control group (P<0.05). 24 hours after transfection, the relative expression of miR-1298 in mimic group cells was significantly higher than that in mimicNC group ( P<0.05). The cells apoptosis rate in mimic group was lower than that in mimicNC group, while cells apoptosis rate in inhibitor group was higher than that in inhibitor NC group (all P<0.05). The expression of BCL-2 in mimic group was higher than that in mimicNC group, while the expression of BAX decreased. Compared with the inhibitorNC group, the expression of BCL-2 decreased, while the expression of BAX increased, and the differences were statistically significant (all P<0.05). miR-1298 alleviates the injury of OGD/R in PC-12 cells by inhibiting apoptosis. [ABSTRACT FROM AUTHOR]

Copyright of China Mechanical Engineering is the property of Editorial Board of China Mechanical Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of CT Theory & Applications is the property of Editorial Department of CT Theory & Applications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)