Objective To explore the extraction process of liposoluble constituents of Codonopsis pilosula and analyze the liposoluble constituents of Codonopsis pilosula. Methods After using single factor analysis and uniform design experimentation to optimize the four parameters of extraction temperature, liquid to material ratio, extraction time, and ultrasound power for the liposoluble constituents of Codonopsis pilosula, the ether ultrasound assisted extraction method was used to extract the liposoluble constituents of Codonopsis pilosula. The content of liposoluble constituents of Codonopsis pilosula from different producing regions was compared, and the changes in liposoluble constituents of Wushan Miaoyu Codonopsis pilosula (referred to as Miao Dang) were analyzed. Results (1) Within the extraction time range of 10-50 minutes, the extraction rate of liposoluble constituents showed an increasing trend with the increase of time. When the extraction time exceeds 50 minutes, the extraction rate began to decrease. (2) Within the extraction temperature range of 15-45 ℃, the extraction rate of liposoluble constituents showed an increasing trend with the increase of extraction temperature, When the extraction temperature exceeds 45 ℃, the extraction rate began to decrease. (3) Within the material liquid ratio range of 1∶5-1∶10, the extraction rate of liposoluble constituents showed an increasing trend. When the material liquid ratio exceeds 1∶10, the extraction rate began to decrease. (4) Within the ultrasound power range of 80-120 W, the extraction rate of liposoluble constituents showed an increasing trend. When the ultrasound power reached 120 W, the extraction rate reached the maximum value of 2.072 6%, and then the extraction rate began to decrease. There were significantly differences in the content of liposoluble constituents in Codonopsis pilosula from 10 different places of production. After being stored at low temperature (4 ℃) for one month, the liposoluble constituents of Miao Dang decreased from 90 to 29, and the types of components underwent great changes. Conclusion The use of ether ultrasound assisted extraction method to extract the liposoluble constituents of Codonopsis pilosula has the characteristics of stable, efficient, and reliable process. There are significant differences in the content of liposoluble constituents of Codonopsis pilosula from different places of production, with the highest content being found in the Miao Dang. This partially explains the strong odor of Miao Dang. At the same time, attention should be paid to the storage conditions and time for the liposoluble constituents of Miao Dang. [ABSTRACT FROM AUTHOR]