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Copyright of China Rubber Industry is the property of Editorial Office of China Rubber Industry and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the regulation of phosphoglycerate kinase 1 (PGK1) on B-catenin and its effect on the invasiveness of glioma. Methods SiRNA and overexpression vector were used to interfere with the expression level of PGK1 in glioma U87 cells, which was verified by Western blot and qRT-PCR. The co-localization expression of PGK1 and B-catenin was detected by fluorescence double staining. The regulation of PGK1 on the expression of B-catenin was observed, and the changes of growth, proliferation, invasion and migration of U87 cells under different expression of PGK1 and B-catenin were detected. In U87 cells with different PGK1 expression levels, the expression levels of B-catenin at different phosphorylation sites were detected by Western blot. Results After transfection with PGK1 siRNA overexpression vector, the growth activity, invasion and migration of U87 cells decreased or increased accordingly. PGK1 could affect the expression of B-catenin. The results of fluorescence double staining suggested that the co localization region of PGK1 and B-catenin had the same direction change. PGK1 could affect the expression of phosphorylated active form of B-catenin. The treatment of glioma cells with B-catenin inhibitor (XAV 939) had no effect on the expression of upstream PGK1, but the cell growth activity decreased and the number of invasive and migratory cells decreased. Conclusions PGK1 can affect the expression of ß-catenin in glioma cells. They can promote the proliferation, migration, invasion and growth of glioma cells. The mechanism may be related to the phosphorylation of B-catenin induced by PGK1. [ABSTRACT FROM AUTHOR]

Copyright of China Plastics / Zhongguo Suliao is the property of Journal Office of CHINA PLASTICS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] This paper aimed to isolate and identify the pathogen species of Pseudobagrus fulvidraco in Tongwang farm of Han chuan city, Hubei province, and the characteristics of its effects on the expression of antimicrobial peptide gene in host were analyzed, so as to provide a research basis for the prevention and treatment of the disease. [Method] The pathogen PA-5 from the liver of diseased Pel teobagrus fulvidraco by line culture was isolated. Gram staining and microscopic observation showed that the morphology of the pathogen was rod-shaped and gram-negative. The pathogen was sensitive to d-Gluconic acid, malonic acid and glucose, but insensitive to myxin, aescin and arabinose. The 16S rRNA and gyrB gene sequences of the strain were analyzed by multiplex PCR. [Result] It was found that the charac teristic sequence of the strain was similar to that of P. aeruginosa, the genetic relationship was close, and after synthesis, the pathogen was identified as P. aeruginosa. At the same time, the expression of antimicrobial peptide gene PCl / PC2 / PC3 in the host was analyzed after the pathogen was infected with P.fulvidraco. P. aeruginosa PA5 ( 3. 0 x 106 CFU/ mL) was injected into the abdominal cavity of P. fulvidraco, and the liver, kidney and epidermis of P. fulvidraco were dissected 0 - 96 hours after infection. Then the total RNA of P. fulvidraco was obtained by Trizol kit, the cDNA was synthesized by reverse transcription, and the expres sion characteristics of antibacterial peptide gene PCl / PC2 / PC3 gene in P. fulvidraco was analyzed by real-time fluorescent quantitative PCR. After P. aeruginosa PA-5 infected P. fulvidraco, the rela tive expression of antimicrobial peptide gene PCl / PC2 / PC3 in liver tissue was higher than that in control group at 24 and 36 hours ( P < 0. 05) ; The relative expression level of antimicrobial peptide gene PCl / PC2 / PC3 in host kidney tissue was higher than that in control group at 24 and 36 hours (P < 0.05); The relative expression of antimicrobial peptide gene PCl / PC2 / PC3 in host epidermis was 36, 48 hours was higher than that in the control group ( P < 0. 05) . The expression of antimicrobial peptide gene in liver, kidney and epidermis increased rapidly, but the reaction time of liver and kidney was earlier than that of epidermis. [Conclusion] Therefore, P. aeruginosa PA-5 was the main pathogen of P. fulvidraco in Tongwang farm of Hanchuan city, Hubei province. The host antimicrobial peptide gene PC1/PC2/ PC3 would respond positively to the infection of P. aeruginosa in Tongwang farm of Hanchuan city, Hubei province, which were conductive to antimicrobial infection. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Medical Equipment Journal is the property of Chinese Medical Equipment Journal Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Clinical Neurosurgery / Linchuang Shenjingwaike Zazhi is the property of Editorial Board of Journal of Clinical Neurosurgery and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】 The biological adsorbent in the treatment of the heavy metal pollution of chromium and zinc from wastewater had the characteristics of cheap raw materials, simple operation and high adsorption efficiency, and the purpose of this study was to find an adsorbent with excellent efficacy from forestry wastes. 【Method】The present paper selected acidified pine powder as an adsorbent material, using sulfuric acid for acidification treatment on its surface, and the p H value and temperature of the solution, Cr(VI) and Zn(II) initial concentration, adsorbent dosage and adsorption time on the adsorption of Cr(VI) and Zn(II) ion efficiency were studied. 【Result】The acidified pine powder had the optimal conditions for adsorption of Cr(VI) and Zn(II) ion from wastewater of p H2.0, temperature of 40 °C, chromium and zinc ion initial concentration of 20 mg/L, adsorbent dosage of 1.0 g/L and adsorption time of 120 min. The analysis of the adsorption kinetics showed that the adsorption of Cr(VI) and Zn(II) ions followed the pseudo-second-order kinetic equation. The analysis of the adsorption isotherm model showed that the adsorption of Cr(VI) and Zn(II) ions followed the Langmuir adsorption isotherm model. 【Conclusion】 The adsorption effect of acidified pine powder on Cr(VI) and Zn(II) ions from wastewater was perfect. The adsorption occurred mainly as the chemical adsorption of single molecule layer on the surface of acidified pine powder. [ABSTRACT FROM AUTHOR]

[Objective]The present paper aimed to study the causes of pathogenic bacteria and its effect on the immune factor. [Method]U-sing the streak plate culture, the pathogen named XF-6 was isolated and purified in the liver of Tenualosa reevesii through VITEK-32 automatic analyzer with detection of bacteria belonging to Aeromonas hydrophila. A multiple PGR method was used to eonfinn that Aeromonas hy-drophila XF-6 contained the gas soluble gene (aerA) and the hemolysin gene (ahhl), belonging to the hemolytic Aeromonas hydrophila. Phe effects on the immune function of Tenualosa reevesii with the infection of Aeromonas hydrophila XF-6 were confirmed by the injection of 4.0 x 105cfu/inL of Aeromonas hydrophila XF-6. The blood and liver of Tenualosa reevesii was collected after 6 -96 hours infection, using speetrophotometrie detection method to calculate the concentration of iron, using ICP-AES to calculate the iron concentration in liver, using the QR-PCR method to calculated the change of iron regulated genes, including hejK-idin gene (hepi;), interleukin (il-6) gene and protein tyrosine kinase (Jak3) gene, transeription factor gene (stat3). [Result] By the infection of Aeromonas hydrophila XF-6, the iron concentration in Tenualosa reevesii 1)I(kxI decreased in 12, 24, 48 hours; The concentration of Blood iron protein increased But did not reach significant difference; The iron concentration in liver increased But did not reach significant difference. The expression of liver liepcidin gene reached significant difference in 6, 12, 24 hours; Liver expression of interleukin gene reached significantly in 12, 24, 48 hours; Liver tyrosine kinase gene expression reached significant differ- ences in 12,24 hours; The expression of liver activator gene reached significant differences in 6, 12, 24 hours. [Conclusion] Therefore, after the infection of Aeromonas hydrophila XF-6 in Tenualosa reevesii, hepcidin gene (hepc), interleukinprotein tyrosine kinase (jak3) gene and transcription factor gene (stat3) expression were increased, thereby reducing the blood iron concentration, increasing blood ferritin, increasing hepatic iron concentration and improving the immune capacity. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Clinical Neurosurgery / Linchuang Shenjingwaike Zazhi is the property of Editorial Board of Journal of Clinical Neurosurgery and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)