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Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] The Vacuum infiltration transformation method is simple, fast and effective because of non-tissue culture. This paper first worldwide reported a new transformation method in rice-vacuum infiltration transformation method. [Method] Using a set of special e-quipment designed by ourselves, the most robust plants in vivo before flowering were vacuum infiltrated insuspension, Sophora Flavevescens Lectin (SFL) was successfully introduced into rice. [Result] A large number of T0 transgenic seeds were harvested. A number of transgenic plants were gained by Basta resistance selection, ehlomphenol-red (CPR) and PCR determination suggested that the SFL gene had been integrated into the rice genome. The transgenic plants showed high blast resistance by means of the seedling spray inoculation in the field compared to non-transgenic rice. This implied that SFL gene was expressed in transgenic rice plants. [Conclusion] Vacuum infiltration transformation method provided a rapid and effective new approach for genetic transformation in rice and other cereal crops. At the same time, it also laid the foundation for research on the SFL gene function and breeding of high blast resistant rice varieties in the future. [ABSTRACT FROM AUTHOR]