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【Objective】The present paper aimed to investigate the species of parasitic ticks on the surface of yaks in Luhuo county, Sichuan province, and the infection status of Rickettsia (SFGR) and invisible ticks.【Method】Ticks were collected from the surface of yaks in six villages in Luhuo county. After preliminary morphological identification, tick genomic DNA was extracted. PCR technology was used to amplify partial fragments of tick ITS-2, SFGR OmpB, and amorphous 16S rRNA genes. The positive products were sequenced, compared, and an evolutionary tree was constructed to determine the species of ticks and their infection status with SFGR and anaplasma.【Result】A total of 820 adult ticks were collected with only Haemaphysalis qinghaiensisus (29/820) and Rhipicephalus microplus (791/820) found. Rickettsia spp. were detected in all six villages and total infection rate was 49.8% (408/820) with only Candidatus R. longicornii detected. The infection rates of SFGR in six villages were statistically significant (χ² = 111.524, P = 0.000 ＜ 0.01). The infection rates of SFGR in H. qinghaiensisus and R. microplus were 44.8% (13/29) and 49.9% (395/791), respectively with no significant differences (χ² = 0.292, P = 0.589 ＞ 0.5). Anaplasma spp. (including A. bovis, A. marginale, A. platys, and A. phagocytophilum) were detected in five villages, with a total infection rate of 30.9% (253/820). The infection rates of Anaplasma in five villages were statistically significant (χ² = 139.825, P = 0.000 ＜ 0.01). Also, the infection rates of Anaplasma between H. qinghaiensisus and R. microplus were statistically significant (χ² = 8.087, P = 0.004 ＜ 0.01). Co-infection of SFGR and Anaplasma was observed with the infection rate of 18.8% (154/820).【Conclusion】There are Qinghai blood tick and micro fanhead tick in Luhuo county, which also carry SFGR and various intangible organisms. Therefore, it is necessary to do a good job in the prevention and control of local tick borne spotted fever group rickettsia and intangible organisms. [ABSTRACT FROM AUTHOR]

【Objective】In order to provide the data for establishment of diagnosis method on bovine Schistosomiasis by analyzing component of soluble egg antigen of Schistosoma japonicum. 【Method】In this study, isolation and identification of SEA were performed with SDS-PAGE, western blot, Chromatographic separation with sephadex and Dot-ELISA. 【Result】Totally ten proteins bands were detected by SDS-PAGE, including 100,80,60,55,45,28,27,17,15 and 13 KDa; Three proteins bands(50,28,17 KDa) were detected by western blot, strong signals were observed on 28 and 17 KDa protein bands, and weak signal was observed on 50 KDa protein band-two protein peaks [including protein molecules of high molecular weight(I peak) and low molecular weight(II peak), respectively]were observed with gel filtration on Sephadex G-200; High speed centrifugation was conducted for proteins separation of II peak and supernatant proteins were specific antigens for diagnosis of bovine schistosomiasis confirmed by Dot-ELISA.【Conclusion】In summary, after high speed centrifugation, supernatant proteins of II peak were specific and natural antigens for diagnosis of bovine schistosomiasis [ABSTRACT FROM AUTHOR]

[Objective] This paper aimed to investigate the prevalence of parasitic worms in gastrointestinal tract of goats in Panzhihua. [Method] Between December 2017 and December 2018, the morphological and molecular identification were performed on worms isolated from gastrointestinal tract of goats slaughtered in different areas of Panzhihua, which were sorted. [Result]The six species of worms were detected, including five species of nematodes(Oesophagostomuma sperum, Bunostomum trigonocephalum, Haemonchus contortus, Trichuris ovis and Chabertia ovina) and one trematode(Eurytrema pancreaticum). There was a mixed infection of trematode and nemaode, mainly between 2 and 3 species of worms.Phylogenetic analysis showed that the variability of COI gene of Haemonchus contortus isolates in Panzhihua were significantly different, ranging from 2.4% to 5.1 %. [Conclusion] In summary, the results of this study showed that there was a high level of mixed infection of gastrointestinal worms in goats of Panzhihua. It was necessary to further strengthen the surveillance and comprehensive prevention and control. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aims to explore the pathogenicity and immunogenicity of Eimeria magna isolate in Sichuan province. 【Method】 Eimeria magna from our laboratory were used to infect experimental rabbits. 【Result】 In the pathogenicity assay, the slight decreases of appetite in SPF rabbits infected w ith 5 x 103 and 5 x 104 sporulated oocysts during 4-9 days after the infection were observed, respectively；Mild diarrhea of rabbits infected with 5 x 105 oocysts during 6 - 8 days after the infection lasted for 3 days, and the significant difference of mean body weight per rabbit were observed compared with control group (P ≤ 0. 05), however, no death in all groups was found. 5 x 103 oocysts were necessary to attain the maximum oocyst output (2 . 31 x 108 ). In the immunogenicity assay, only 6 . 28 x 104 oocysts in rabbits immunised with 5 x 103 sporulated oocysts after challenge w ith 7 x 105 oocysts were detected. Compared with control group, no significant difference in body weight gain in other groups was found. 【Conclusion】 The E. magna isolate had low virulence but immunogenicity in rabbit, indicating its feasibility as a vaccine component. [ABSTRACT FROM AUTHOR]