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Copyright of Feed Industry is the property of Liaoning Provincial Institute of Agricultural Mechanization and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】This study established the technical system of musk deer marker assisted selection to provide a theoretical basis for the breeding of forest musk deer. 【Method】The basic functions and protein structure of forest musk deer insulin-like growth factor( IGF-1) by gene cloning were analyzed, the polymorphism of IGF-1 gene by PCR-SSCP technology was detected, and its correlation effect on the number of litter was explored. 【Result】(i) The sequence of IGF-1 gene was 465 bp, encoding 153 amino acid residues, and the bioinformatics analysis revealed that the protein contains 15 phosphorylation sites, a secreted protein mostly located in the cell membrane, containing a conserved Insulin family of typical domain;(ii) The length of PCR-SSCP amplified fragment of IGF-1 gene was 300 bp, the primer amplified fragment was detected by 10 % polyacrylamide gel, and it was found that there was no specific band in the amplified fragment. In order to prove the result of PCR-SSCP amplification, one of the primers was selected to amplify the hair samples, and sequencing showed that all PCR products had a complete sequence and no polymorphism. 【Conclusion】This research showed that the IGF-1 gene was not chosen as a molecular genetic marker of forest musk deer, which would provide a basic idea for the breeding of molecular genetic markers of incense production performance of forest musk deer. [ABSTRACT FROM AUTHOR]