1. 水力负荷对内循环生物脱氮装置脱氮效能影响及其机理.
- Author
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郑毅凯, 李 甍, 孙佐梁, 宋协法, 李 贤, and 董登攀
- Subjects
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NITRITE reductase , *NITRATE reductase , *NITRIFYING bacteria , *DENITRIFYING bacteria , *POISONS - Abstract
In Recirculating Aquaculture Systems (RAS), nitrifying bacteria in biofilters are responsible for converting toxic substances such as organic nitrogen, NH+4-N, and NO-2-N in the culture water into less toxic NO-3-N. However, the high dissolved oxygen concentration and low carbon-to-nitrogen ratio in the system limit the denitrification performance of the biofilters, leading to the accumulation of NO-3-N. This accumulation not only poses a threat to the health of cultured animals but also presents challenges for the compliant discharge of aquaculture tailwater. This study employs an internal recirculating biological denitrification device to treat marine RAS wastewater and investigates water quality changes under different Hydraulic Loading Rate (HLR) conditions, specifically setting HLR at 1 m³/(m²·d), 2 m³/(m²·d), and 3 m³/(m²·d). At the end of each experimental stage, samples of PCL and K3 fillers from the device were collected for high-throughput sequencing to analyze the characteristics of their microbial communities and the results of functional gene predictions. The results indicate that during stable operation of the device, the average removal rate of NO-3-N was highest at 67. 31%±3. 03% when the HLR was 2 m³/(m²·d). The removal load of NO-3-N increased with the rise of HLR, reaching 55. 38±6. 13 g·N/(m³·d) at an HLR of 3 m³/(m²·d). During the stable operation of the device, no accumulation of NH+4-N, NO-2-N, and COD was detected in the effluent, with the main denitrification area located in the inner circle of the device. At the phylum level, Proteobacteria was the dominant bacterial phylum in both PCL and K3 samples. At the genus level, the denitrifying unclassified_f__ Rhodobacteraceae and Ruegeria dominated the biofilms on both types of fillers. The abundance of unclassified_ f__Rhodobacteraceae in the PCL samples increased with the increase in HLR, whereas the abundance of Ruegeria increased as the HLR decreased. Functional gene prediction results indicated that the abundance of nitrate reductase (EC 1. 7. 99. 4), nitrite reductase (EC 1. 7. 2. 1), nitric oxide reductase (EC 1. 7. 2. 5), and nitrous oxide reductase (EC 1. 7. 2. 4) involved in denitrification significantly increased with the rise in HLR. In conclusion, this study demonstrates that the biological denitrification device performs best at an HLR of 2 m³/(m²·d). It is recommended to consider this design in practical production and focus on colonizing and cultivating the two efficient denitrifying bacteria, unclassified_f__Rhodobacteraceae and Ruegeria, in the device. This research provides important references for optimizing the hydraulic load rate design of biological denitrification devices and offers new insights for further studies on denitrification efficiency. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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