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Objective To explore the molecular mechanism of miR-125b promoting invasion, metastasis and epithelial-mesenchymal transition (EMT) of gastric cancer cells. Methods The expression of miR-125b in gastric cancer and its adjacent tissues was studied by qRT-PCR. After upregulating or downregulating miR-125b in gastric cancer cells, the protein expressions of DKK3 and SERPINA4 were detected by Western blotting. Dual luciferase reporting assay was used to verify whether miR-125b can target DKK3 and SERPINA4. MKN45 cells were co-transfected with miR-125b inhibitor and target gene siRNA. Migration and invasion experiments were conducted to explore whether miR-125b can regulate the biological function of MKN45 cells through DKK3 and SERPINA4. Then, the regulatory mechanism of SRF on miR-125b was investigated. Finally, by in vivo experiments, the expression of SRF in gastric cancer cells was upregulated or downregulated by lentivirus transfection; the number of lung metastases in nude mice was detected to explore the effect of SRF on gastric cancer cell metastasis. Results In this study, the expression of miR-125b increased in gastric cancer tissues, which was correlated with clinical stage and lymph node metastasis. Dual luciferase reporting experiments showed that DKK3 and SERPINA4 were the direct targets of miR-125b in gastric cancer cells, and could activate the Wnt/β-catenin signaling pathway, thereby promoting the transcription process of EMT-related transcription factors Twist1 and Slug, inducing the occurrence of EMT, and promoting the metastasis of gastric cancer. In vitro and in vivo experiments confirmed that SRF promoted the invasion and metastasis of gastric cancer cells by positively regulating the expression of miR-125b. Conclusion Taken together, SRF/miR-125b axis promotes the EMT and metastasis of gastric cancer cells, and these regulators represent new potential therapeutic targets or biomarkers for gastric cancer. [ABSTRACT FROM AUTHOR]

Copyright of Railway Standard Design is the property of Railway Standard Design Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study conducted a survey on 950 rural older adults aged 60 years and above in rural areas of Hefei City by using multi - stage stratified cluster random sampling, to describe the fall status of the rural older adults and analyze the influencing factors of falls, therefore provides a reference for the prevention of falls among older adults. The questionnaire included general demographic information, behavior, living environment, frailty, etc., and the occurrence of falls, including the types, time, place and reasons for falls. The incidence of falls in the past year was 26.84% among the rural older adults in Hefei City. Most of them fell outdoors (70.20%), mostly in the morning (53.72%). Multivariate logistic regression analysis showed that women, non- living alone, had depressive symptoms, weak body condition, suffered from stroke had a high risk of falling. Older people aged 80 years and older had a lower risk of falling. On the whole, the fall rate of older adults in the rural areas of Hefei City is high and is affected by many factors. Multiple measures should be taken targeting key populations to reduce the fall risk specifically, therefore improving the quality of life and happiness of rural older adults in their later years. [ABSTRACT FROM AUTHOR]

The rural revitalization strategy is the key for China to promote sustainable economic development, coordinate regional development and ultimately achieve common prosperity in the coming period. Relying on their advantages in scientific research, education, capital, expertise, and personnel training, colleges and universities have become the core force driving the comprehensive implementation of the rural revitalization strategy. For many universities in Guangxi with tourism-related majors, although they have advantages in professional application, talent intelligence, education and training and tourism data in helping the implementation of the rural revitalization strategy, they still face problems such as inadequate policy implementation, poor coordination mechanism, limited service capacity, unreasonable research platform and team. Based on this, this paper proposes that under the guidance of the concept of symbiosis and integration, measures such as establishing the service concept of systematic symbiosis, building the discipline system of co-construction and sharing, expanding the service field of co-development and resonance, strengthening the service talents of symbiosis and win-win can provide a strong guarantee for the implementation of the rural revitalization strategy of tourism colleges in the area. [ABSTRACT FROM AUTHOR]

Copyright of China Mechanical Engineering is the property of Editorial Board of China Mechanical Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aiming at the problems of many types of goods and high labor consumption in the current logistics field, in order to meet the needs of rapid sorting of production lines, a 2-CPR/ RPU parallel mechanism with full-cycle freedom was proposed to improve the sorting efficiency of logistics goods and reduce the labor time cost. The three-dimensional model of 2-CPR/ RPU mechanism was drawn, and the degree of freedom of 2-CPR/ RPU mechanism was solved by screw theory. According to the modified Grübler-Kutzbach formula, the position inverse solution of the branch chain of the mechanism was analyzed by closed vector. Based on the inverse solution expression, the velocity Jacobian matrix of the mechanism was obtained. The algebraic method was used to analyze whether the singular configuration exists. The MATLAB language was programmed to solve the points that meet the working range and draw the working space. Finally, the finite element simulation analysis of the bearing weight of the mechanism was carried out. The results show that the 2-CPR/ RPU parallel mechanism has a total of three degrees of freedom, which can realize 2T1R type motion (translation along the x, z axis and rotation around the x axis). The structure size meets the requirements without singular configuration, the working space is large, evenly distributed and fan-shaped symmetry, and the rotation of the execution platform can reach 60°. The 2-CPR/ RPU parallel mechanism has simple structure, stable motion, high working accuracy and good load performance, which is suitable for sorting and packing in most logistics packaging production lines [ABSTRACT FROM AUTHOR]

Copyright of Railway Signalling & Communication Engineering is the property of Railway Signalling & Communication Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electric Power is the property of Electric Power Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Advances in New & Renewable Energy is the property of Editorial Office of Advances in New & Renewable Energy and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Ordnance Equipment Engineering is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of China Medical University is the property of Journal of China Medical University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Tropical Geography is the property of Tropical Geography Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to obtain the critical hydraulic gradient for the incipient motion of particles, based on the geometric boundary conditions of the variable cross-section porous pipe, the dynamic analysis of the incipient motion of movable particles in the general existence state and the clogged particles in the critical existence state was carried out, respectively, and the different factors that might affect the incipient motion of particles under the two states were considered. A theoretical model for predicting the critical hydraulic gradient of particle incipient motion was established, and the validity of the model for predicting the critical hydraulic gradient was verified by the existing experimental data. The study started from the actual state of the particles in the pore pipe, introduced key influencing factors such as the relative shielding degree, the relative exposure degree and stress state for mechanical analysis, and explored the effect of parameters such as seepage direction, the relative shielding degree, the relative exposure degree on the critical hydraulic gradient. The obtained calculation model is of great significance to the rationality and reliability of the prediction of critical hydraulic gradient. [ABSTRACT FROM AUTHOR]

Copyright of Petroleum Geology & Oilfield Development in Daqing is the property of Editorial Department of Petroleum Geology & Oilfield Development in Daqing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Advances in New & Renewable Energy is the property of Editorial Office of Advances in New & Renewable Energy and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Railway Signalling & Communication Engineering is the property of Railway Signalling & Communication Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Railway Signalling & Communication Engineering is the property of Railway Signalling & Communication Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Science & Technology (0253-2336) is the property of Coal Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To investigate the gender difference in structure, composition and diversity of intestinal microbiota in captive sika deer(Cervus nippon), 16S rRNA V3-V4 region amplicons in the feces of 10 captive healthy sika deer were amplified by PCR using the Illumina MiSeq high-throughput sequencing platform. The structure, diversity, and different species of the intestinal microbiota of male and female captive sika deer were analyzed by bioinformatics method, with gender as a grouping factor. The results showed that at the phylum level, the dominant phyla of intestinal microbiota of captive sika deer were Firmicutes (61. 69% in males and 58. 23% in females) and Bacteroidetes(27. 40% in males and 31. 19% in females). At the genus level, the dominant genera were Bacteroides (4. 60% in males and 6. 13% in females) and Clostridium_IV (3. 42% in males and 3. 25% in females). There was no significant difference in alpha diversity of male and female captive sika deer (p>0. 05). Beta diversity analysis showed that the composition of sika deer microbiota of different genders was similar, and there was no significant difference between groups(p>0. 05). STAMP and LEfSe analysis showed that Bacteroidetes played an important role in female captive sika deer(LDA value>4, p<0. 05). This study reveals that there is no significant gender induced difference in the structure and diversity of intestinal microbiota in captive sika deer. However, the distribution of individual bacteria phyla and genera in the intestine of male and female sika deer are different, and Bacteroidetes are greatly differed by gender of the host. [ABSTRACT FROM AUTHOR]

Copyright of Tunnel Construction / Suidao Jianshe (Zhong-Yingwen Ban) is the property of Tunnel Construction Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Microplastics are high molecular-weight polymers with micro- or nano-meter size, which have become a major global environmental issue in recent decades due to their ubiquity in the environment, bioavailability and potential toxicity. New analytical methods for the characterization of microplastics and nanoplastics are quickly emerging, such as particle size measurement, component identification and quantification. Many techniques have been developed and applied for the characterization of microplastics, such as Fourier-transform infrared spectroscopy, Raman spectroscopy and mass spectrometry (MS), etc. In recent years, the continuous development of MS technology has drawn more and more attention to the characterization and the environmental impact of microplastics. This paper mainly reviewed the state-of-the-art development and application of MS in microplastics analysis during the past decade. The principles and characteristics of various MS techniques used in the qualitative and quantitative analysis of microplastics were described, including thermal analysis coupled with MS-based techniques, elemental analytical MS, MALDI-ToF MS, LC-MS and ToF-SIMS. MS can provide chemical structure, molecular weight, degree of polymerization, additives, main functional groups and other information about the polymers, so it can be widely applied for chemical characterization, particle size determination and semi-quantitative/quantitative analysis of microplastics of small size. It has the advantages of replicable, fast real-time in situ analysis and high throughput detection, which can not only detect microplastics in the environment but also in biological tissues or cells in situ, showing great application potential in the research on the source, migration, transformation and interaction with biological organisms. The microplastics have been detected in the ocean, atmosphere, soil, consumer products, organisms and even human tissue, including polyethylene, polyamide, polyvinyl chloride, polypropylene, polystyrene, polycarbonate, polymethyl methacrylate, polyethylene terephthalate, etc. The health risks of ingestion of microplastics deserve further exploration, especially nano plastics. The continuous development of MS technique and its combination with other separation/characterization methods will greatly promote the analysis of microplastics, and MS will become a more and more powerful tool in this research field and provide important scientific basis for the control of microplastic pollution. [ABSTRACT FROM AUTHOR]

Copyright of Tropical Geography is the property of Tropical Geography Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Frontiers of Computer Science & Technology is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Zhengzhou University (Natural Science Edition) is the property of Journal of Zhengzhou University (Natural Science Edition) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective/Meaning】The pastoral complex is the carrier of the effective implementation of the rural revitalization strategy, and also an important starting point to promote the reform of the agricultural supply side. By summarizing and analyzing the development path and mode of the pastoral complex model, the case practice and experience reference were provided for building the pastoral complex with its own characteristics in different regions of China.【 Methods/Procedures】 Through the analysis of the concept of new pastoralism, the construction and operation status of the first batch of 17 pastoral complexes in Guilin were systematically sorted out. Then, based on the field investigation and data collation, the development stage, main characteristics and construction mode of the pastoral complex in Guilin were analyzed and summarized.【 Results/Conclusions】 The results showed that the construction of pastoral complex in Guilin was still in the initial stage, and the government leading occupied a large proportion. The main types and construction modes included five types, such as the agriculture-based type, scenic spot-driven type, urban-based type, cultural-based type, and experience-based type. In view of the problems existing in the construction and operation of different types of pastoral complex, the strategies for the development of pastoral complex in Guilin in the future were put forward, including promoting the integration of three industries according to the local conditions, constantly innovating the land use mechanism, building the professional team of management talents, and excavating the local culture to build the local characteristic brand, etc. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Aesthetic & Plastic Surgery is the property of Chinese Journal of Aesthetic & Plastic Surgery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Ordnance Equipment Engineering is the property of Chongqing University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Application Research of Computers / Jisuanji Yingyong Yanjiu is the property of Application Research of Computers Edition and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Oncology is the property of Chinese Journal of Oncology / Zhonghua Zhongliu Zazhi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Aesthetic & Plastic Surgery is the property of Chinese Journal of Aesthetic & Plastic Surgery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Beijing University of Technology is the property of Journal of Beijing University of Technology, Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Frontiers of Computer Science & Technology is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Refrigeration is the property of Journal of Refrigeration Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Railway Standard Design is the property of Railway Standard Design Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)