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The experiment was to study the effect of fructooligosaccharides replacing chloramphenicol on growth performance, apparent digestibility of nutrients and serum biochemical indexes of weaned piglets. A total of 48 weaned piglets were randomly divided into three groups, piglets in control group were fed with basal diet, and the experimental groups were supplemented with 25 mg/kg aureomycin and 5 000 mg/kg fructooligosaccharide in basic diet, respectively, with eight replicates in each group and two piglets in each replicate. The experiment lasted for 28 d. The results showed that compared with control group, the final weight and average daily gain of piglets in aureomycin and fructooligosaccharide groups on 1~14, 1~28 d were significantly increased (P<0.05), and the diarrhea rate and feed to gain ratio were significantly decreased (P<0.05). On 1~28 d, the digestibility of crude protein and total energy of piglets in aureomycin group and fructooligosaccharide group was significantly increased (P<0.05). On 14 d, serum immunoglobulin G and immunoglobulin A content of piglets in aureomycin group and fructooligosaccharide group were significantly increased (P<0.05), total superoxide dismutase activity and total antioxidant capacity of serum were significantly increased (P<0.05), and malondialdehyde content was significantly decreased (P<0.05). On 28 d, compared with control group, the serum immunoglobulin A content and malondialdehyde content of piglets in aureomycin group and fructooligosaccharide group were significantly increased (P<0.05), and serum immunoglobulin G content and total superoxide dismutase activity in fructooligosaccharide group were significantly increased (P<0.05). The study indicates that dietary fructooligosaccharide can enhance antioxidant level, improve immunity, reduce diarrhea rate, improve digestibility, and then improve growth performance of piglets. Fructooligosaccharide can be used to replace auretein in production. [ABSTRACT FROM AUTHOR]