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Objective: To explore the construction and application of the multi-course evaluation system of Pathogen Biology and Immunology under the mode of online and offline mixed teaching. Methods: A total of 104 students from two classes of clinical major of Grade 2022 in Quanzhou Medical College were studied, and combined online and offline teaching mode was adopted for teaching. A curriculum evaluation system was constructed, including process evaluation, assessment evaluation and summative evaluation. Selfefficacy, self-learning ability, course questionnaire and other aspects were statistically analyzed before and after the course. Results: The students' normal grades could be reflected objectively by the new curriculum assessment and evaluation system. The final comprehensive grades were better, and the course passing rate was higher. The paired t-test analysis demonstrated statistically significant improvements in students' learning motivation, learning management, and self-efficacy after the course（ P<0.05）. However, the differences in information literacy and cooperation ability were not statistically significant compared with those before the course （P>0.05）. Course questionnaire survey found that 89.4% students had a high evaluation of the new course evaluation system, which was conducive to promoting learning. Conclusion: The newly constructed curriculum assessment system is more scientific and reasonable, which is conducive to improving students' self-efficacy and autonomous learning ability, and promoting students' learning. [ABSTRACT FROM AUTHOR]