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Copyright of Chinese Journal of Dermatovenereology is the property of Xi'an Jiaotong University Periodicals Center and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Cardiac dysfunction due to spinal cord injury is an important factor of death in patients with spinal cord injury; however, the specific mechanism is still not clear. Therefore, revealing the mechanism of cardiac dysfunction in spinal cord injury patients is of great significance to improve their quality of life and survival rate. OBJECTIVE: To investigate the mechanism of luteolin in improving serum-induced myocardial cell death in spinal cord injury rats. METHODS: Allen’s impact instrument was used to damage the spine T9-T11 of male SD rats to establish a spinal cord injury model meanwhile a sham operation group was set as the control group. The serum of rats of each group was collected. H9c2 cells were divided into a blank control group, a sham operated rat serum group, a spinal cord injury rat serum group and a luteolin pretreatment group. The cells in blank control group were only cultured with ordinary culture medium. The cells in the sham operated rat serum group were treated with medium containing 10% serum from sham operated rat. The cells in the spinal cord injury rat serum group were treated with medium containing 10% serum from spinal cord injury rat. The cells in the luteolin pretreatment group were precultured with a final concentration of 20 μmol/L luteolin for 4 hours and then changed to a medium containing 10% rat serum from spinal cord injury rat. After 24 hours of culture, the survival rate of each group of H9c2 cells was measured by CCK-8 assay. Western blot assay was used to detect the expression of autophagy related protein LC3 and p62 in H9c2 cells in each group. RESULTS AND CONCLUSION: Compared with the blank control group, there was no significant change in cell survival rate in the sham operated rat serum group (P > 0.05). Compared with the sham operated rat serum group, the cell survival rate (P < 0.01) and the expression of LC3 protein (P < 0.05) in spinal cord injury rat serum group was significantly reduced, and the expression of p62 protein was significantly increased (P < 0.05). Compared with the spinal cord injury rat serum group, the survival rate of cells in the luteolin pretreatment group significantly increased (P < 0.000 1); the expression of LC3 protein significantly increased (P < 0.05), and the expression of p62 protein significantly decreased (P < 0.05). The results indicate that luteolin may improve myocardial cell death induced by serum from rats with spinal cord injury by promoting autophagy. [ABSTRACT FROM AUTHOR]

Objective: To discuss the improved methods for the isolation and culture of primary chondrocytes from the neonatal rats, and to establish an efficient and economical in vitro chondrocyte culture system. Methods: The primary chondrocytes were isolated from the joints of neonatal rats and divided into overnight digestion (OD) group and rapid digestion (RD) group for separation. The chondrocytes in OD group were digested overnight by type Ⅱ collagenase, while the chondrocytes in RD group were separated by the combination of pre-digestion with physical and chemical digestion methods. The chondrocytes were cultured in modified media containing 0% (blank group 1), 1%, 2%, 4%, and 10% fetal bovine serum (FBS), 0 (blank group 2), 0. 1, 0. 2, 0. 4, 0. 8, 1. 0, and 2. 0 g·L-1 vitamin C (VC), and 0 (blank group 3), 0. 5, 1. 0, 2. 0, 4. 0, 8. 0, 10. 0 μg·L-1 poly (lactic-co-glycolic acid ) (PLGA) nanoparticles. The media containing different concentrations of FBS, VC, and PLGA were mixed with Dulbecco's modified Eagle's medium/nutrient mixture F-12 (DMEM/F12), and were divided into related groups based on the concentrations of ingredients. Cell counter was used to count the chondrocytes in various groups and the survival rates and diameters of the chondrocytes in various groups were detected; Toluidine blue staining was used to detect the morphology of the chondrocytes in various groups; CCK-8 method was used to detect the proliferative activities of the chondrocytes in various groups; cell adhesion assay was used to detect the adhesion rates of the chondrocytes in various groups; Hoechst/propidium iodide (PI) staining was used to detect the apoptosis of the chondrocytes in various groups; MTT assay was used to detect the proliferation activities of the chondrocytes in various groups after treated with modified media. The cells were divided into DMEM/F12+10%FBS group, DMEM/F12+1%FBS group, and DMEM/F12+1% FBS+0. 4 g·L-1 VC+1 μg·L-1 PLGA group. Real-time fluorescence quantitative PCR (RT-qPCR) method was used to detect the expression levels of sex-determining region Y-box 9 (SOX9), collagen type Ⅱ alpha 1 chain (Col2A1), collagen type Ⅹ alpha 1 chain (Col10A1), and matrix metallopeptidase 13 (MMP13) mRNAs in the chondrocytes in various groups after treated with modified media; immunofluorescence staining was used to detect the expressions of type Ⅱ collagen (COLⅡ) and SOX9 in the chondrocytes in various groups after treated with modified media. Results: The survival rate of primary chondrocytes in OD group was lower than that in RD group, and the average cell diameter was larger than that in RD group. The primary chondrocytes in OD group were larger and spindle-shaped, and most cells exhibited pseudopodia; in RD group, the primary chondrocytes were smaller, mostly rhomboid in shape, with only a portion of the cells showing pseudopodia. The Toluidine blue staining results showed significant coloration in both groups, but the digestion time of the chondrocytes in RD group was shorter, and compared with OD group, the actual culture time of the chondrocytes was reduced by 9-13 h, and more immature morphology of the primary chondrocytes were observed. The proliferation activity of the primary chondrocytes in OD group was slow at 24 h of culture but increased at 48 h of culture, and the proliferation activity of the primary chondrocytes was significantly higher at 48 h of culture compared with 12 h of culture (P<0. 01). Compared with 12 h of culture, the proliferation rates of the primary chondrocytes in RD group were increased at 24 and 48 h of culture (P<0. 01). At 24 and 48 h of culture, compared with OD group, the proliferation rates of the primary chondrocytes in RD group were increased (P<0. 05). The number of apoptotic chondrocytes in RD group was lower than that in OD group, and no necrotic chondrocytes were observed in either group. The proliferation activities of chondrocytes of the rats were increased with the rising of FBS concentration in the culture medium. Compared with blank group 1, the proliferation activities of chondrocytes of the rats after treated with culture mediums containing 1%, 2%, 4%, and 10% FBS were significantly increased (P<0. 05). Compared with blank group 2, the proliferative activities of chondrocytes of the rats after treated with culture mediums containing 0. 2-1. 0 g·L-1 VC were significantly increased (P<0. 05), and the highest proliferation activity was found when the concentration of VC was 0. 4 g·L-1 (P<0. 01). Compared with blank group 3, the proliferation activities of chondrocytes of the rats after treated with culture mediums containing 1-4 μg·L-1 PLGA were significantly increased (P<0. 05), and the highest proliferation activity was found after treated with culture medium containing 1 μg·L-1 PLGA (P<0. 05). Compared with DMEM/F12+10%FBS group, the expression levels of SOX9 mRNA and Col2A1 mRNA in the chondrocytes in DMEM/F12+1%FBS group were significantly increased (P<0. 05 or P<0. 01). Compared with DMEM/F12+10%FBS group, the expression levels of SOX9 mRNA and Col2A1 mRNA in the chondrocytes in DMEM/F12+1%FBS+0. 4 g·L-1 VC+1 μg·L-1 PLGA group were significantly increased (P<0. 01). The immunofluorescence staining results showed that the green fluorescence signal of COLⅡ and the red fluorescence signal of SOX9 were observed in some chondrocytes in DMEM/F12+10%FBS group under fluorescence microscope, and the fluorescence intensity was weak. In DMEM/F12+1%FBS group, most chondrocytes exhibited COL Ⅱ green fluorescence signal and SOX9 red fluorescence signal, and the fluorescence intensity was significantly stronger than that in DMEM/F12+10% FBS group. In DMEM/F12+1% FBS+0. 4 g·L-1 VC+1 μg·L-1 PLGA group, the COLⅡ green fluorescence signal and SOX9 red fluorescence signal were found in all the chondrocytes, and the fluorescence intensity was significantly higher than those in DMEM/F12+10%FBS and DMEM/F12+1%FBS groups. The expression levels of COLⅡ and SOX9 proteins in the chondrocytes in DMEM/F12+1%FBS group were significantly higher than those in DMEM/F12+10%FBS group, and the expression levels of COLⅡ and SOX9 proteins in the chondrocytes in DMEM/F12+1%FBS+ 0. 4 g·L-1 VC+ 1 μg·L-1 PLGA group were significantly higher than those in DMEM/F12+10%FBS group. Conclusion: The improved methods for the isolation and culture of primary chondrocytes of the rats can overcome the shortcomings of traditional methods, shorten the isolation time of primary chondrocytes, and improve the quality of in vitro culture of primary chondrocytes. [ABSTRACT FROM AUTHOR]

Copyright of Acta Prataculturae Sinica is the property of Acta Prataculturae Sinica Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study evaluated the protein identification capabilities of the Top14 high-abundance protein removal kit (Top14) and the DMB MagicOmics low-abundance enrichment kit (DMB) on serum samples from healthy individuals (HC) and hepatocellular carcinoma (HCC) patients. For HC samples, DMB treatment led to identifying 2.6 times proteins more than Top14 and 3.9 times more than untreated samples. For HCC samples, DMB achieved 3.7 times increase in protein identification over Top14 and 6.2 times increase over untreated samples. Although the Top14 kit was effective in removing high-abundance proteins, it was less proficient in detecting low-abundance proteins when compared to the DMB method. In terms of protein identification frequency, the DMB-treated samples had a significantly higher number of quantifiable proteins than both the Top14 and Blank groups. Over 50% of the proteins identified in the Top14 and Blank groups were also identified in the DMB group, ensuring a comprehensive proteome coverage as evidenced by KEGG analysis. The DMB method significantly outperformed the others in HCC serum samples, identifying 47 differentially expressed (DE) proteins, in contrast to 15 and 17 identified of the Top14 group and untreated samples, respectively, highlighting its superior ability to uncover critical biomarkers for disease analysis. KEGG pathway analysis showed that DE proteins identified by DMB were involved in 21 distinct pathways, significantly more than 5 and 1 pathways identified by Top14 group and untreated samples, demonstrating DMB’s advanced proteomic profiling capability. This study also underscored HSP90AB1, SPP1, ACTR3, SNCA, PECAM1, and SRC proteins increased in HCC serum samples based on DMB method, marking them as promising HCC biomarkers for disease screening. [ABSTRACT FROM AUTHOR]

Objective To explore potential predictive biomarkers for preeclampsia by analyzing the expression and characteristics of vascular-related inflammatory proteins in the serum of preeclamptic pregnant women. Methods A case-control study was conducted from Sep 2017 to Sep 2018 in the Obstetrics and Gynecology Hospital, Fudan University. Preeclamptic pregnant women and normotensive pregnant controls during the same period were recruited. The concentration of 12 vascular inflammation-related proteins in the patients’ serum was determined by multiplex flow cytometry. The vascular-related inflammatory molecules were subjected to differential analysis by non-parametric t-test to obtain preeclampsia-related vascular inflammatory proteins. Finally, high-risk preeclampsia pregnancy serum samples from the biobank were retrieved. Serum concentrations of preeclampsia-related vascular inflammatory proteins were determined, and receiver operating characteristic (ROC) curve analysis was conducted for preliminary predictive assessment of preeclampsia. Results This study conducted a quantitative analysis of serum vascular inflammatory proteins in 39 cases of preeclampsia and 43 cases of control pregnant women, detecting 10 vascular inflammatory proteins expressed in maternal serum. Among these, myoglobin and vascular cell adhesion molecule-1 (VCAM-1) exhibited significant elevation in the serum of preeclamptic pregnant women, while matrix metalloproteinase-9 (MMP-9) showed a marked decrease. ROC curve analysis revealed that in 96 high-risk early pregnancy women, the area under the curve (AUC) for VCAM-1 reached 0.802, whereas myoglobin and MMP-9 did not significantly predict the occurrence of preeclampsia. Conclusion Preeclampsia patients exhibit abnormal expression of serum inflammatory proteins, among which VCAM-1 may be a potential biological marker for early pregnancy preeclampsia risk prediction. [ABSTRACT FROM AUTHOR]

The purpose of this study was to study the effect of supplementation of Eucommia ulmoides leaves in the diet on serum biochemical indexes and rumen fermentation parameters of finishing beef cattle. The study selected seventy-five local cattle × Simmental hybrid bulls with an initial body weight of （252.67±6.00） kg. They were divided into five treatment groups by a randomized block test design, each treatment group had 3 replicates, and each replicate had five cattle. The 0 group was fed a basal diet with a concentration-to-crude ratio of 3∶7, and the four study groups were supplemented with 1%, 2%, 3%, and 4% Eucommia ulmoides leaves in the basal diet to replace the forage in the basal diet. The pre-trial period is 15 days, and the trial period is 150 days. The results showed that ①there were no significant differences in serum TP, ALB, GLO, A/G, BUN, TC, TG, HDL-C, LDL-C content of beef cattle, as well as the activities of ALT, AST and ALP in serum among the treatment groups (P>0.05). ②Compared with 0 group, the levels of complement C3, complement C4 and IgA in the beef cattle serum of 2% group increased significantly (P< 0.05), which were 101.24%, 79.13% and 102.61% higher than 0 group, respectively, and the differences among the other groups were not significant(P>0.05). The serum IgG and IgM contents were not significantly different among the groups (P>0.05), but the IgG and IgM contents in the 2% group were increased by 44.43% and 75.50%, respectively, compared with 0 group. Serum SOD, T-AOC, MDA, GSHPx levels were not significantly different among the groups (P>0.05). ③Compared with 0 group, the concentration of NH3-N in the rumen liquid of beef cattle in the 1% and 4% groups was significantly reduced (P<0.05). However, there were no significant differences in the pH, TVFA, acetic acid concentration, propionic acid concentration, butyric acid concentration and Acetic/Propionic Acid in the rumen liquid of beef cattle among the treatment groups (P>0.05). In conclusion, under the study conditions, adding Eucommia ulmoides leaves to the diet of beef cattle had no significant effect on serum biochemical indexes and antioxidant indexes, but the addition of 2% can increase the content of complement C3, C4, IgA, IgG, and IgM in serum, and reduce the concentration of ammonia nitrogen in rumen liquid. [ABSTRACT FROM AUTHOR]

The objective was to determine the optimum effective phosphorus demand for juvenile snakeheads and to investigate the effects of different levels of NaH2PO4 on the growth, serum biochemical parameters and nutrient composition of juvenile snakeheads. Six diets with different levels of NaH2PO4 (0.41%, 0.64%, 0.86%, 1.09%, 1.31% and 1.57%, the 0.41% group being the control group) were prepared and fed to juvenile snakeheads for 8 weeks, with three replicates of 540 fish in each group, to determine their growth, serum biochemical parameters and nutrient composition. Specific growth rates, protein efficiency and whole fish phosphorus content were fitted separately using a regression linear model to determine their optimum effective phosphorus requirements. The results showed that specific growth rate and protein efficiency ratio were obvious higher than those of the control group when the available phosphorus content rise to 0.86% and 1.09%, respectively, and the feed efficiency showed the same trend (P<0.05); the viscerosomatic index showed general downward trend when upward trend occurs in available phosphorus concentration (P<0.05). The phosphorus content of whole fish rise evidently at 1.09% of the available phosphorus content, then start to ebb gradually. With the continuous addition of the available phosphorus in dietary, the ash and phosphorus were moved up slightly which deposit in vertebrae and scales (P<0.05). In contrast, the crude lipid content of the control group was evidently higher than added group (P<0.05). LDL-C, HDL-C, TC and TG decreased evidently with the increase of available phosphorus content in the dietary (P<0.05). Superoxide dismutase activity increased with increasing available phosphorus in the dietary(P<0.05). Based on the parabolic regression analysis of specific growth rate, protein efficiency, and total phosphorus content in snakehead, the optimal effective phosphorus contents were determined to be 0.98%, 1.06%, and 1.19% respectively. To reduce the risk of eutrophication in the aquaculture environment, the recommended available phosphorus content for snakehead dietary is 0.98%. [ABSTRACT FROM AUTHOR]

Obejective: To investigate the expression and clinical significance of long non-coding RNA lung adenocarcinoma metastasis-associated lung adenocarcinoma transcript 1 (LncRNA MALAT1) and microRNA-769-5p (miR-769-5p) in serum and gingival crevicular fluid of patients with chronic periodontitis. Methods: 117 patients with chronic periodontitis who were admitted to our hospital from June 2020 to July 2023 were selected as observation group, patients were divided into 39 cases in mild group, 42 cases in moderate group and 36 cases in severe group according to the severity of chronic periodontitis, another 117 healthy volunteers were selected as control group. The expression levels of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid were compared between control group and observation group. The expression levels of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid, the levels of inflammatory indexes [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interferon-γ (IFN-γ)] in serum and gingival crevicular fluid, periodontal indexes [periodontal pocket depth (PD), attachment loss (AL), gingival crevicular bleeding index (SBI), plaque index (PLI)] were compared among different disease severity. The correlation between inflammatory indicators, periodontal indicators and the expression levels of miR-769-5p and LncRNA MALAT1 in serum and gingival crevicular fluid in patients with chronic periodontitis were analyzed by Pearson correlation analysis. The diagnostic value of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid for chronic periodontitis were analyzed by receiver operating characteristic (ROC) curve. Results: The expression levels of LncRNA MALAT1 in serum and gingival crevicular fluid in observation group were higher than those in control group (P<0.05), and the expression levels of miR-769-5p in serum and gingival crevicular fluid were lower than those in control group (P<0.05). The expression levels of LncRNA MALAT1 in serum and gingival crevicular fluid and IL-6, TNF-α, IFN-γ, PD, AL, SBI and PLI in mild group were lower than those in moderate group (P<0.05), and moderate group were lower than those of severe group (P<0.05). The expression levels of miR-769-5p in serum and gingival crevicular fluid were higher in mild group than in moderate group (P<0.05), and moderate group were higher than those of severe group (P<0.05). The expression level of LncRNA MALAT1 in serum and gingival crevicular fluid of patients with chronic periodontitis was positively correlated with IL-6, TNF-α, IFN-γ, PD, AL, SBI and PLI (P<0.05). The expression level of miR-769-5p in serum and gingival crevicular fluid was negatively correlated with IL-6, TNF-α, IFN-γ, PD, AL, SBI and PLI (P<0.05). The expression level of LncRNA MALAT1 in serum and gingival crevicular fluid was negatively correlated with the expression level of miR-769-5p (P<0.05). The area under the curve (AUC) of serum LncRNA MALAT1, miR-769-5p and combined detection in the diagnosis of chronic periodontitis were 0.800, 0.743 and 0.877 respectively. The AUC of gingival crevicular fluid LncRNA MALAT1, miR-769-5p and combined detection in the diagnosis of chronic periodontitis were 0.786, 0.849 and 0.891 respectively. Conclusion: The expression level of LncRNA MALAT1 in serum and gingival crevicular fluid of patients with chronic periodontitis is increase, and the expression level of miR-769-5p is decrease, which can aggravate the inflammatory reaction and periodontitis symptoms, the combine detection of LncRNA MALAT1 and miR-769-5p in serum and gingival crevicular fluid has high diagnostic value for chronic periodontitis. [ABSTRACT FROM AUTHOR]

Objective: To analyze the correlation between the expression levels of IL-17, IL-21 and IL-22 in serum and placenta of preterm pregnant women and neonatal infection. Methods: 120 preterm parturients who delivered in our hospital from July 2020 to December 2021 were selected as the observation group, and 120 full-term parturients in the same period were selected as the control group. The expression levels of IL-17, IL-21 and IL-22 in serum and placenta of mothers in the two groups were detected. According to whether newborns delivered by mothers in the observation group were infected or not, they were divided into infected group and non-infected group. The expression levels of IL-17, IL-21 and IL-22 in serum and placenta of mothers in the two groups were compared. Multivariate Logistic regression analysis and receiver operating characteristic curve (ROC) were used to analyze the relationship between serum and placental IL-17, IL-21 and IL-22 and neonatal infection. Results: The expression levels of IL-17, IL-21 and IL-22 in serum and placenta of observation group were higher than those of control group(P<0.05). The expression levels of IL-17, IL-21 and IL-22 in serum and placenta of infected group were higher than those of non-infected group(P<0.05). Multivariate Logistic regression analysis showed that serum and placental IL-17, IL-21 and IL-22 were independent predictors of neonatal infection (P<0.05). According to Pearson correlation analysis, there were positive correlations between serum IL-17 and placental IL-17 mRNA, serum IL-21 and placental IL-21 mRNA, and serum IL-22 and placental IL-22 mRNA in preterm parturients (P<0.05). According to ROC curve analysis, the AUC of serum IL-17 and IL-21 combined with IL-22 for predicting neonatal infection was 0.910. Conclusion: The elevated levels of IL-17, IL-21 and IL-22 in serum and placenta of preterm parturients are closely related to the occurrence of neonatal infection, and the efficacy of serum IL-17, IL-21 and IL-22 combined with IL-22 in predicting neonatal infection is higher, which is worthy of further study and application. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Hydrobiology / Shuisheng Shengwu Xuebao is the property of Editorial Department of Journal of Hydrobiology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background Schizophrenia is a common severe mental disorder with complex pathogenesis. There are few studies on the correlation between kynurenine metabolites in peripheral serum and urine in schizophrenia. Objective To investigate the concentration of tryptophan-kynurenine metabolites and interleukin-6(IL-6) in serum and urine in patients with schizophrenia, and their correlation with clinical symptoms, so as to explore potential biological characteristics related to schizophrenia. Methods A total of 38 patients with schizophrenia who met the criteria of the Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5), and were hospitalized or attended outpatient clinic at Hangzhou Seventh People's Hospital from December 2021 to December 2022 were included in the study. Additionally,26 healthy individuals were concurrently recruited from the community of Hangzhou to serve as a control group. All participants were requested to complete the Positive and Negative Symptom Scale (PANSS) . The levels of tryptophan (TRP), kynurenine (KYN), kynurenic acid (KYNA), quinolinic acid (QUIN), picolinic acid (PIC), xanthurenate and 5-hydroxytryptamine (5-HT) in both serum and urine were measured using ultra-high-performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry. Serum and urine IL-6 were measured using enzyme-linked immunosorbent assay. Pearson correlation analysis was conducted to examine the correlation between serum and urinary KYN metabolites, as well as the correlation between metabolite levels and clinical symptoms in the patient group. Results Patients with schizophrenia had significantly higher level of IL-6 in serum (U=798. 500,P<0. 01) and lower level of PIC in urine (U=253. 000, P=0. 013) compared with the control group. Additionally, level of serum KYN was positively correlated with QUIN/KYNA ratio and QUIN/PIC ratio (r=0. 562,0. 438,P<0. 05) in patients with schizophrenia. 5-HT/KYN ratio in serum was positively correlated with PANSS total score and negative symptom subscale score (r=0. 458,0. 455,P<0. 01) in patients with schizophrenia. Conclusion Serum TRP-KYN pathway metabolite levels in patients with schizophrenia were associated with neurotoxic metabolite ratios in urine and the severity of negative symptoms. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Inorganic Analytical Chemistry / Zhongguo Wuji Fenxi Huaxue is the property of Beijing Research Institute of Mining & Metallurgy Technology Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: The aggregates formed after translational modification of α-synuclein are the main pathological changes of Parkinson’s disease. α-Synuclein can penetrate the blood-brain barrier and be delivered from the central nervous system to the peripheral blood to all parts of the body, which becomes an important pathway for the pathological dissemination of Parkinson’s disease. Therefore, it is particularly important to study the changes of blood markers in patients with Parkinson’s disease to reveal the mechanism of Parkinson‘s disease as well as for early diagnosis. OBJECTIVE: To analyze the differences in the structural stability of phosphorylation modification sites and generated aggregates of α-synuclein in serum of patients with Parkinson’s disease. METHODS: A recombinant human α-synuclein prokaryotic expression system was constructed, and the protein was purified by affinity chromatography. The purity and specificity of α-synuclein monomer was detected by SDS-PAGE and western blot. Serum samples of 26 normal controls and 26 patients with Parkinson’s disease in the Department of Neurology, Affiliated Hospital of Beihua University were collected to complete the preparation of serum α-synuclein aggregates. The modified sites for phosphorylation modification of α-synuclein protein in normal serum and serum of patients with Parkinson’s disease were identified using SWATH-mass spectrometry, and protein aggregates at different sites were quantitatively analyzed. The protein aggregates with different phosphorylation modifications were purified by immunoaffinity chromatography, and then detected for stability in the serum of patients with Parkinson’s disease by western blot. RESULTS AND CONCLUSION: The results of SDS-PAGE and western blot showed that α-synuclein monomers with high purity and specificity were obtained. The results of the SWATH-mass spectrometry analysis showed that phosphorylation modifications occurred in both Parkinson’s disease patients and normal human serum, with significantly more phosphorylated sites in Parkinson’s disease patients than in normal humans. The phosphorylation sites in the serum of patients with Parkinson’s disease were Serine (Ser) 87, Ser129, L-tyrosine (Tyr)125, Tyr 133 and Tyr 136. After incubation in the serum of patients with Parkinson’s disease for α-synuclein, the phosphorylation modification at Ser129 accounted for 53.65% of the total phosphorylation, with 17.21%, 15.79%, 15.79%, 9.52%, and 1.03% for Tyr125, Tyr133, Tyr136, and Ser87, respectively. The serum levels of Ser129 phosphorylation-modified aggregates were detected by western blot to be more stable than those of Tyr125, Tyr133 and Tyr136 in patients with Parkinson’s disease. To conclude, the number of α-synuclein phosphorylation modification sites in the serum of patients with Parkinson’s disease was significantly higher than that of normal subjects. The aggregate structure generated by phosphorylation modification at Ser129 in the serum of patients with Parkinson’s disease was the most stable. This may provide a diagnostic marker for the early diagnosis of Parkinson’s disease. [ABSTRACT FROM AUTHOR]

BACKGROUND: Rheumatoid arthritis is a common autoimmune disease that mainly affects the joints and is characterized by persistent synovial inflammation. Studies have suggested that proinflammatory pyroptosis is closely related to the occurrence and development of rheumatoid arthritis. OBJECTIVE: To investigate the expression levels of pyroptosis-related factors NOD-like receptor protein 3, cysteine aspartic acid-specific protease-1 (caspase-1), interleukin-18 and interleukin-1β in serum and articular fluid of patients with rheumatoid arthritis and their clinical significance. METHODS: From January 2021 to January 2022, 24 patients with rheumatoid arthritis admitted to the Rheumatology Department of Luoyang Orthopedic- Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital) were selected as rheumatoid arthritis group. Thirty osteoarthritis patients with knee joint effusion were selected as osteoarthritis group. Forty healthy subjects were selected as control group. C-reactive protein level, erythrocyte sedimentation rate, anti-cyclic citrullinated peptide antibody level, rheumatoid factor levels, and disease activity score in 28 joints were measured and recorded. NOD-like receptor protein 3, caspase-1, interleukin-18 and interleukin-1β levels in serum and joint fluid were determined by enzyme-linked immunosorbent assay (ELISA) to analyze the correlation between their expression levels and the clinical indicators of patients with rheumatoid arthritis. RESULTS AND CONCLUSION: The serum levels of NOD-like receptor protein 3, caspase-1, interleukin-18 and interleukin-1β in the rheumatoid arthritis group were significantly higher than those in the control group (P < 0.0001), while the articular fluid levels of NOD-like receptor protein 3, caspase-1, interleukin-18 and interleukin-1β in the rheumatoid arthritis group were significantly higher than those in the osteoarthritis group (P < 0.05-0.01). The expression levels of NOD-like receptor protein 3, caspase-1, interleukin-18 and interleukin-1β in serum and articular fluid of patients with rheumatoid arthritis were positively correlated with D disease activity score in 28 joints, C-reactive protein level, and erythrocyte sedimentation rate. To conclude, pyroptosis-related factors are significantly increased in patients with rheumatoid arthritis and correlated with disease activity, suggesting that pyroptosis may play a role in the pathogenesis of rheumatoid arthritis. [ABSTRACT FROM AUTHOR]

Objective To explore the correlation between the levels of complement (C3c, C4, C1q) in peripheral blood and the occurrence and development of cataract in patients with age-related cataracts (ARC). Methods This is a case-control study. Subjects diagnosed with ARC between 2016 and 2020 were recruited from Eye & ENT Hospital of Fudan University. Age- and gender-matched control group was recruited during the same period. The basic information were collected. Serum levels of C3c, C4, C1q were measured by Roche automatic biochemical analyzer. The independent student’s t-test was used for the comparison of C3, C4 and C1q between the patients and the control group.The associations between C3c, C4, C1q and ARC were analyzed by Spearman correlation and binary logistic regression. Results A total of 2 321 ARC patients (male 1 115, female 1 206) and 2 281 controls (male 1 112, female 1 169) were enrolled in this study. Independent sample t-test showed that the serum levels of C3c, C4 (104. 13±17.28 mg/dL, 26.85±7.99 mg/dL) were significantly lower in the ARC group compared with the control group (112.81±17.96 mg/dL, 28.08±8.37 mg/dL); the serum level of C1q (219.27±65.03 mg/L) were significantly higher in the ARC group compared with the control group (207.87±48.49 mg/L) (P<0.05). Correlation analysis showed that the level of serum C1q was postively correlated with ARC (r=0.062, P<0.001 ), while the levels of serum C3c (r=-0.243, P<0.001), C4( r=-0.081, P<0.001) were negatively correlated with ARC (P <0.05). Logistic regression analysis showed that the increased serum level of C1q (male: OR=1.007, 95%CI=1.005~1.008; female: OR= 1.001, 95%CI= 1.000~1.003) was the risk factor for the occurrence of ARC (P<0.05). Conclusions The levels of C3c, C4 and C1q in peripheral blood of ARC subjects were significantly changed and the increased serum level of C1q may be the risk factor for ARC. It indicated that complement system might be involved in the pathogenesis of ARC. [ABSTRACT FROM AUTHOR]

The aim of this experiment was to explore the effects of 1.5%-8% hybrid Broussonetia papyrifera leaves on the production performance, intestinal tissue morphology, digestive enzyme activity, serum antioxidant and biochemical indexes after replacing part of corn and soybean meal in the basic diet of Dawu Golden Phoenix laying hens at the early stage of laying eggs. At the end of 147 day old, tatol of 540 Dawu Golden Phoenix laying hens were selected, which were randomly divided into six groups (each group has six replicates, with 15 hens per replicate): fed them with basic diets containing 0, 1.5%, 2.5%, 3.5%, 4.5%, and 8% hybrid Broussonetia papyrifera leaves replacement ratios, respectively. After the 7-day pre-test period, the daily production performance of the laying hens was counted with the unit of repetition during the formal test period (a total of 56 days). At the end of the for⁃ mal test period, the intestinal tissue morphology, digestive enzyme activity, and serum antioxidant and biochemica indexes of laying hens were measured. The results showed that: compered with the control grouop, all experimental groups were able to significantly reduce the total antioxidant capacity in the serum of laying hens (P<0.01); 1.5% group can significantly increase the total protein in the serum of laying hens (P<0.01); 2.5% group can significantly increase the malonaldehyde, amylase and inorganic phosphorus values in the serum of laying hens (P<0.01); 3.5% group can effectively improve the morphology of duodenum, jejunum and ileum of laying hens, and can significantly increase the activity of amylase in duodenum (P<0.01); the effect of 4.5% group is the best to reduce the mortality rate, feed to egg ratio, abnormal egg rate, and increase the laying rate, average egg weight, and qualified egg rate, and can significantly increase the activities of trypsin, chymotrypsin, and lipase in the duodenum of laying hens (P< 0.01), as well as the glucose value in serum; the total bilirubin value in serum of laying hens was significantly increased by 8% group. The appropriate replacement feeding ratio of hybrid Broussonetia papyrifera leaves can effectively improve the intestinal tissue morphology, production performance, digestive enzyme activity, serum antioxidant and biochemical indexes of laying hens. The replacement feeding ratio of 3.5%-4.5% has the best comprehensive effect and is most suitable for application in production. [ABSTRACT FROM AUTHOR]

Objective: To compare the advantages and disadvantages of boiling method and phenol-chloroform method for extraction of HBV-DNA in serum and exosomes, Taqman method and SYBR qPCR for detection of HBV-DNA expression, and search for more accurate and sensitive diagnosis and treatment reference index. Methods: Collected serum samples from high viral load and low viral load groups, extracted serum exosomes and identified them. Then, extracted HBV-DNA from both serum and exosomes, and compared expression differences between boiling method and phenol-chloroform method for extraction, as well as qPCR Taqman method and SYBR method for detection. Results: In high viral load group （HBV-DNA>5E+02 U/ml）, serum HBV-DNA was extracted by boiling method, Taqman method was higher than SYBR method（P<0.05）; Taqman was used to detect HBV-DNA in serum, SYBR was used to detect HBV-DNA in exosomes, the extraction efficiency of boiling method was both higher than that of phenol-chloroform method（P<0.05）; HBV-DNA expression in serum was higher than that in exosomes（P<0.05）. In low viral load group （HBV-DNA< 5E+02 U/ml）, there was no statistical difference between boiling method and phenol-chloroform method, and between Taqman method and SYBR method for serum and exosome HBV-DNA qPCR detection. HBV-DNA expression in exosomes was higher than that in serum（ P<0.05）. Conclusion: Both extraction and detection methods have the same sensitivity and specificity. Level of HBV-DNA encapsulated by exosomes may be an indispensable key factor in clinical diagnosis and treatment of chronic hepatitis B. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the expression and clinical significance of soluble leukocyte differentiation antigen 14 (sCD14) and soluble leukocyte differentiation antigen 163 (sCD163) in patients with lupus nephritis (LN). Methods: 90 patients with LN who were the Department of Nephrology in our hospital from December 2019 to June 2022 were selected. According to the systemic lupus erythematosus disease activity index 2000 (SLEDAI-2000) score, patients with LN were divided into active stage LN group (greater than4 scores, 53 cases) and non-active stage LN group (less than or equal to 4 scores, 37 cases), another 45 healthy volunteers who underwent physical examination in outpatient department of our hospital were selected as control group. The levels of serum and urinary sCD14 and sCD163 of each group were detected, and their correlation with SLEDAI-2000 score and routine laboratory indicators was analyzed. Multivariate Logistic regression analysis was used to analyze the risk factors of patients with LN in active disease stage. Results: The levels of serum and urinary sCD14, serum and urinary sCD163 in the active stage LN group were higher than those in the non-active stage LN group and control group (P＜0.05), and the levels of serum and urinary sCD14, serum and urinary sCD163 in the non-active stage LN group were higher than those in the control group (P＜0.05). The levels of serum and urinary sCD14, serum and urinary sCD163 in patients with LN were positively correlated with SLEDAI-2000 score, urinary protein, urea nitrogen and creatinine levels (P＜0.05). Multivariate Logistic regression analysis showed that increased serum and urinary sCD14, serum and urinary sCD163 and urinary protein levels were risk factors for patients with LN in the disease active stage (P＜0.05). Conclusion: The levels of serum and urinary sCD14 and sCD163 are significantly increased in patients with LN in the disease active stage, and high levels of sCD14 and sCD163 are associated with renal impairment and increased disease activity in patients with LN. [ABSTRACT FROM AUTHOR]

Metabolomics is a technology which understands the changes of all endogenous metabolites in the organism at a certain time through comprehensive and quantitative detection of endogenous small molecular compounds in the biological system, screening out significant biomarkers, and providing new ideas for early prediction, diagnosis and exploration of pathogenesis of diseases. Missed abortion is a common gynecological disease, and its pathogenesis has not been fully elucidated. At present, metabonomics technology has been applied to the study of missed abortion, and biomarkers that may be associated with missed abortion have been found, involving a variety of pathways such as tricarboxylic acid cycle, oxidative stress and signal transduction, making a breakthrough. This article briefly introduces the metabonomics technology and its application in the study of missed abortion, and to further discusses the explore of differential metabolites in serum and changes in related metabolic pathways on missed abortion. [ABSTRACT FROM AUTHOR]

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Objective To explore the value of the levels of human complement fragment 4d ( C4d ) in renal tissues, serum and urine in evaluating the condition, curative effect and prognosis of patients with IgA nephropathy( IgAN). Meth⁃ ods Totally 214 patients with primary IgAN were selected. The renal tissue was taken by percutaneous renal biopsy, and the expression of C4d was observed by immunohistochemistry. We collected the patients' serum and urine, and detected the level of C4d by ELISA. The clinical data of patients with different levels of C4d expression in the renal tissues, serum and urine were compared. After 6 to 8 months of treatment with ACEI/ARB combined with immunosuppressive agents, the therapeutic effect was observed. The correlation between the expression of C4d in the renal tissues, serum and urine and the therapeutic effect of IgAN patients was analyzed by Spearman correlation analysis. CDK staging of renal function was per⁃ formed on the deadline of follow-up to compare the difference in the expression of C4d in the renal tissues, serum and urine of patients in different stages of renal disease. Results The expression of C4d in the renal tissues was positive in 76 cases and negative in 138 cases. The expression level of serum C4d was( 1 270. 27 ± 332. 99) ng/mL, and the expression level of urine C4d was( 297. 53 ± 45. 53) ng/mL. Compared with renal C4d -negative patients, the renal C4d -positive patients had higher 24-hour urinary protein and lower serum albumin( both P<0. 05). Compared with patients with low expression of se⁃ rum C4d , patients with high expression of serum C4d had higher 24-hour urinary protein and lower serum albumin( both P <0. 05). Compared with patients with low expression of urine C4d , patients with high expression of urine C4d had higher MAP, 24-hour urine protein content, and Scr, and lower eGFR and serum albumin( all P<0. 05), and had more severe glomerular pathological changes. After treatment, there were 143 cases of complete remission, 28 cases of partial remis⁃ sion, and 43 cases of no response( ineffective+end-stage renal disease) in 214 patients. Correlation analysis showed that the increased C4d levels in the renal tissues, serum, and urine were negatively correlated with the treatment effectiveness of IgAN patients( rs =-0. 234, -0. 270, and -0. 324, all P<0. 01), with urine C4d having the strongest correlation with treat⁃ ment effectiveness. As of the follow-up date, there were 57 cases of stage 1, 43 cases of stage 2, 31 cases of stage 3, 34 cases of stage 4, and 23 cases of stage 5 in the CDK staging of renal function of IgAN patients. Correlation analysis showed that the increased C4d levels in the renal tissues, serum, and urine were positively correlated with the staging of renal dis⁃ ease in IgAN patients( rs =0. 252, 0. 202, and 0. 431, P<0. 05 or P<0. 01, respectively), with urine C4d having the stron⁃ gest correlation with the stage of renal disease. Conclusion The levels of C4d in the renal tissues, serum and urine have certain correlation with the condition, curative effect and prognosis of IgAN patients, and the relationship with urine C4d is the closest. [ABSTRACT FROM AUTHOR]

Copyright of South China Fisheries Science is the property of South China Fisheries Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Imaging Science & Photochemistry is the property of Imaging Science & Photochemistry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the distribution status, characteristics and clinical significance of quantitative detection modes of five makers of hepatitis B[hepatitis B virus (HBV) surface antigen (HBsAg), anti-HBV surface antibody (HBsAb), anti-HBV e antigen (HBeAg), anti-HBV e antibody (HBeAb) and anti-HBV core antibody (HbcAb) ]in the hospital, so as to obtain the prevalence rate of serum markers of HBV infection and realize the scientific management of prevention and treatment of hepatitis B. Methods A total of 16 246 patients in this hospital from October 2020 to October 2021 were selected to be tested for the five makers of hepatitis B using a fully automated chemiluminescent immunoassay analyzer CL6000i. The test results were also categorized and analyzed by different patterns and populations. Results There were 17 patterns in the five makers of hepatitis B in 16 246 patients, including nine common patterns, seven uncommon patterns and one rare pattern. In 16246 patients with hepatitis B, there were 16 128 common patterns (99. 27%), 114 uncommon patterns (0. 71%) and 4 rare patterns (0. 02%) . HBsAg, HBsAb, HBeAg, HBeAb, HBeAb and HBcAb positive rates were 10. 92% (1 774/16 246), 52. 83% (8 582/16 246), 2. 45% (398/16 246), 25. 70% (4 176/16 246) and 47. 90% (7 782/16 246), respectively. The positive rate of HBsAg in male patients[12. 54% (1 059/8 442) ]was significantly higher than that in females[8. 78% (685/7 804) ] (P<0. 05). The proportion of patients aged 30-<40 and 40-<50 years old was higher, which was 11. 13% and 11. 04%, respectively. Conclusion It is recommended that the results of quantitative detection of hepatitis B should be used for population screening and observation of the curative effect of patients with hepatitis B, so as to realize the scientific management of prevention and treatment of hepatitis B in different populations. [ABSTRACT FROM AUTHOR]

BACKGROUND: The research group has found that the small molecule peptides of antler gum are rich in peptide chains that promote bone growth, proliferation and ossification of bone marrow mesenchymal stem cells. The specific mechanism and target of antler glue in the treatment of avascular necrosis of the femoral head are still unclear, which needs further studies. OBJECTIVE: To explore the mechanism of antler glue in the treatment of femoral head necrosis based on proteomics. METHODS: Thirty-six female Wistar rats, SPF grade, were randomized into a blank group, a model group, and an antler glue group. The rats in the latter two groups were given retinoic acid 70 mg/kg once a day for 8 weeks to establish a rat model of femoral head necrosis, while the rats in the blank group were not treated. At 8 weeks after modeling, the rats in the antler glue group were given antler glue 0.35 g/kg, and those in the model and blank groups were given the same volume of 0.9% sodium chloride solution, once a day by gavage for 8 weeks. Pathological examination of bone tissue was used for verifying the successful modeling and the repair effect of antler glue on the femoral head. Non-standard (Label-free) proteome quantification technology was used to detect differential genes among groups. Biological function of differential proteins and signaling pathways involved were enriched based on GO and KEGG database analyses. STRING database was used to construct the target protein-protein interaction network diagram. The study protocol was approved by the Animal Experiment Ethics Committee of Shandong Academy of Traditional Chinese Medicine. RESULTS AND CONCLUSION: The pathological sections of bone tissue showed that antler glue could effectively repair the necrotic femoral head. A total of 1 457 kinds of proteins were identified, including 353 kinds of common differential proteins between blank group and model group as well as between model group and antler glue group. In the model group, 97 kinds of differential proteins that increased or decreased showed a significant callback after intervention with antler glue. The mechanism of antler glue in the treatment of femoral head necrosis involves blood circulation, energy metabolism, inflammatory reaction and immune process. Protein disulfide isomerase (P4HB), cadherin 2 (CDH2), annexin A2 (ANXA2), proprotein convertase subtilisin/kexin type 9 (PCSK9), milk fat globule epidermal growth factor 8 (MFGE8), and bone morphogenetic protein may be important targets of antler glue. Antler glue may treat femoral head necrosis by regulating transforming growth factor β, glycolysis/gluconeogenesis, and PI3K-Akt signaling pathways. [ABSTRACT FROM AUTHOR]

Selenium is an essential trace element for human body. Insufficient intake of selenium can lead to Keshan disease, Kashin-Beck disease, cardiovascular and other diseases, while excessive intake can lead to selenium poisoning. There are diverse selenium species in the world. Different species of selenium possess different biological activities, migration and transformation rules, and have different effects on nutrition and health. The selenium level of serum is one of the most widely used sample for biological testing of selenium level. In order to study the species and level of selenium in human serum, the chromatographic conditions were optimized. Human serum and without macromolecular protein human serum were selected as samples for ultrasonic enzymatic hydrolysis pretreatment. ZORBAX SB-Aq C18 reversed-phase chromatography column was analysis column, 10 mmol/L citric acid and 5 mmol/L sodium hexane sulfonate (containing 1% methanol, pH 4.0) were mobile phase with isocratic elution. Selenate (Se(Ⅵ)), selenite (Se(Ⅳ)), selenocysteine (SeCys2), methylselenocysteine (MeSeCys) and selenomethionine (SeMet) in human serum samples were determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The results indicated that five selenium species could be separated within 10 min. The linear of five selenium species was good in the range of 0.0-100.0 μg/L, and the correlation coefficients (r) were more than 0.999. The limits of detection were 0.05-0.2 μg/L. The reproducibility of the method was determined by precision. The relative standard deviations (RSDs) were all less than 5%. The standard recovery experiment was carried out by adding three concentration levels and five kinds of selenium form mixed standard solutions. The recoveries of Se(Ⅳ) were 35.1%-39.3% and 87.9%-90.0% for human serum and without macromolecule protein human serum, respectively. The recovery of SeCys2 was 57.7%-72.5%, while the recoveries of Se(Ⅵ), MeSeCys and SeMet were 80%-120%. Analysis of serum in 30 subjects indicated that the main species of selenium was SeCys2 with the concentration of 16.2-29.3 μg/L, followed by SeMet with the concentration of 6.2-16.3 μg/L. Additionally, a small amount of inorganic selenium and unknown selenium species were also determined in serum. Low recovery rate of Se(Ⅳ) in human serum may be due to combination between Se(Ⅳ) and macromolecular protein, which needs to be further studied. [ABSTRACT FROM AUTHOR]

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Objective To investigate the relationship between serum Perlecan DV (PDV) and prognosis after 90 days of acute ischemic stroke. Methods A total of 131 patients with acute ischemic stroke (AIS) admitted to Department of Neurology of the hospital from June 2020 to March 2021 were prospectively selected, and 30 healthy subjects matching t heir gender and age were selected as the control group. Serum PDV levels were determined by an enzyme-linked immunosorbent assay. The prognosis was evaluated using the modified Rankin Scale. Logistic regression was performed to analyze whether PDV was an independent factor influencing the prognosis of AIS. Results Compared with the control group, the level of serum PDV in patients with AIS was significantly increased, and the difference was statisticallly significant (P<0.05) . Serum PDV was not a predictor of prognosis in AIS (OR =0.999, 95% CI:0.996-1.00:3,P >0.05). Conclusion Serum PDV has no significant prognostic value in patients with AIS. [ABSTRACT FROM AUTHOR]

Objective To investigate the mechanism of Jiangu Granule in the treatment of postmenopausal osteoporosis by metabonomics. Methods Sixty rats were randomly divided into normal group, model group and Jiangu Granule group.The Jiangu granule group was given Jiangu granule solution by gavage, and the others were given the same capacity saline by gavage. After 6 week or 12 week, the femur, tibia and lumbar vertebrae were used for the test of bone mineral density(BMD) and biomechanics. The blood of abdominal aorta was analyzed by liquid chromatography-mass spectrometry (LC-MS). After univariate statistics and multivariate statistics analysis, the potential metabolites were selected. Results Compared with the normal group, the BMD and maximum load of model group decreased significantly, while the Jiangu Granule group tended to return to normal level. It was indicated that the model was successful and the rat body was tended to return to normal. The serum metabolic spectrum of the model group and the normal group were completely separated. Jiangu Granule group was close to the normal group. 27 different metabolites, such as pantothenate, stearic acid and corticosterone, were screened out, which were related to the biosynthesis of pantothenate and CoA, unsaturated fatty acids and steroid hormones, respectively. These metabolites were involved in lipid metabolism, nucleic acid metabolism, amino acid metabolism and so on. Conclusion Jiangu granule may treat postmenopausal osteoporosis by regulated multiple metabolic pathways. [ABSTRACT FROM AUTHOR]

A sensitive and fast assay for uric acid analysis was established by using the ratio fluorescence based on nitrogen-doped carbon dots (N-CDs).Uric acid is oxidized to H, 0 2 catalyzed by uricase i- has the same peroxidase-like activity, which could effectively catalyze H 2 0 2 to generate • OH radicals and oxidize o- phenylenediamine ( OPD) to 2, 3-diaminophenazine (DAP) . As a consequence, DAP quenched the fluorescence emission at 427 nm in virtue of inner filter effect (IFE), and produced a new fluorescence emission peak at 580 nm. With the increase of uric acid concentration, the fluorescence intensity decreased at 427 nm, but increased at 580 nm. In the range of 0. 50 µmo! • L -l, the fluorescence intensity ratio (l 580 /I 427 ) of DAP t o N-CDs showed a good linear relationship, and the detection limit (S/N = 3) was as low as 0.06 µmo! • L - l .In addition,the assay was successfully applied to human se rum samples. [ABSTRACT FROM AUTHOR]

Endometriosis (EMs) is a highly recurrent and invasive benign disease, which seriously affects the quality of life of female between puberty and menopause. The lack of effective biomarkers in clinic is one of the important causes leading to the delay of diagnosis. With the progress of the disease, it brings serious health damage and economic burden to patients. At present, laparoscopy is the method for the diagnosis of EMs, but its application in clinic is limited because of its invasiveness, operative complications and high cost. The exploration of biomarkers with good sensitivity and specificity is very important for the non-invasive early diagnosis of EMs. MicroRNA (miRNA) is a kind of non-coding small molecule RNA, with high tissue specificity and stability. Recent studies have shown that miRNA can be used as a potential biomarker for the diagnosis of EMs. Early recognition is helpful for timely diagnosis and treatment of patients. This article reviews the research progress of miRNA in EMs. [ABSTRACT FROM AUTHOR]

A rapid analysis methodology for ten neurotransmitters in serum was developed using ultra performance liquid chromatography-quadrupole-Orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) . A typical protein precipitation (PPT) method was employed for removing protein macromolecular compounds and efficient extraction of the studied compounds. A home-made accurate mass database (massto-charge (m/ z ), retention time, isotopic distribution and characteristic fragment ions) and MS/MS spectra library were used for accurate identification. Quantitative analysis was performed using the extracted m/ z of full-scan MS and external standard method. Matrix effect was 62. 4%-112. 2% and the relative standard deviations (RSDs) were 1. 9%-10. 1%. The extracted recovery and RSDs were 73. 6%-104. 2% and 2. 6%- 1 0. 6 %, respectively. Experimental results indicated that good linearities (R2 > 0. 9 9) were observed in the respective concentration ranges. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 0. 05-11. 63 nmol/L and 0. 09- 46. 50 nmol/L, respectively. Stability of standard solutions, quality control samples and serum samples can meet the requirements of biological samples analysis. Accuracy of the dilution effect (DE) was 5. 1%-10. 6% and the precision was 1. 1%-5. 3%, all within the range of ± 15 % . Carry-over effect ( COE) was 0. 4 %-4. 6 % through t he analysis of initial mobile phase after three consecutive injections of samples of high concentration. Moreover, this method was applied to t he research of neurotransmitters metabolism in the serum of rats with ionizing radiation-induced brain injury. Results demonstrated that ionizing radiation causes neurotransmitter metabolism in serum, and can provide important references for the screening of new biomarkers in serum. [ABSTRACT FROM AUTHOR]

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BACKGROUND: Hypoxia is a common physiological and pathological stress response in various diseases, and exosomes are widely involved in the mechanism of hypoxia injury and its adaptive mechanism. The two are closely related, which is the current research hotspot. OBJECTIVE: To find a new target for the prevention and treatment of high altitude diseases and open up new ideas. METHODS: The PubMed and Medline databases from 2013 to 2020, and the CNKI and Wanfang databases from 2018 to 2019 were searched by computer. The key words were “serum exosomes, hypoxia, tumor, inflammation, cardiovascular and cerebrovascular diseases” in Chinese and English. Finally, 57 articles were included in the review. RESULTS AND CONCLUSION: Hypoxia is a potential environmental death factor by affecting cell cycle, morphology, metabolism, proliferation, differentiation, autophagy, apoptosis and so on. Hypoxia can regulate the release and change the content of exosomes, which is strongly associated with cell specificity, the duration of hypoxia exposure and the severity of hypoxia. In the environment of hypoxia, exosomes can transmit all kinds of biological information and play an important role in biology through specific binding with receptor cells. As a biomarker of disease diagnosis, exosomes have great potential. [ABSTRACT FROM AUTHOR]

The accurate and quantitative analysis of low-abundance proteins in complex matrices has always been the focus and faced difficulty in protein research. With the rapid development of mass spectrometry technology, isotope dilution mass spectrometry has become an important method for accurate quantitative analysis of low-abundance proteins in serum. In this study, isotopically labeled human growth hormone was used as an internal standard. A strategy for separation of serum samples was established based on off-line two-dimensional high-performance liquid chromatography (2D HPLC). Then, combined with high performance liquid chromatography-isotope dilution mass spectrometry ODMS) method, quantitative analysis of low-abundance protein growth hormone (hGH) in human serum was performed with high accuracy. Based on the measurement and uncertainty calculation of the simulated samples (blank serum with known quality of growth hormone standard substance, theoretical concentration 12. 00 ng/ g) and international comparison samples, the quantitative results are (11. 45 ±2. 33) ng/g and (12. 84±1. 46) ng/ g, respectively. This method has high accuracy and good repeatability, and can provide an effective measurement method for accurate quantitative analysis of low-abundance proteins in complex matrices. The results can trace to reference material. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【目的】基于浊点萃取技术（CPE）联合亲水作用色谱法（HILIC）同时测定大鼠血清中四种抗艾滋病药。【方法】以TritonX-114作为萃取介质，用响应面法进一步优化CPE相关参数，对萃取的样品，用HILIC法分别测定其含量。【结果】四种抗艾滋病药在5%（w/v）TritonX-114，0.3mol/L NaCl，pH为5.0，40℃水浴平衡20 min时，萃取率均达85.0%以上，与模型预测值接近；对于富集的样品，以Ze month-CN（250mm×4.6mm，5μm）为固定相，流动相为（甲醇∶乙腈∶醋酸铵缓冲液=5︰5︰90），柱温35℃，检测波长275nm，流速0.5mL/min分离时，四种分析物在0.01～10.0μg/mL，线性良好，加样回收率大于75.0%，RSD≤5.0%。【结论】建立的该方法，操作简单绿色，能对大鼠血清中四种抗艾滋病药较好的富集，可同时快速、准确测定其含量，为其临床血药浓度监测提供新的方法。

Objective To study the expression of heparanase (HPSE) and vascular endothelial growth factor (VEGF) in the serum of patients with colorectal cancer, and analyze their clinicopathological relationship with colorectal cancer. Methods A total of 118 patients with colorectal cancer confirmed by surgery and pathology in the hospital from April 2016 to February 2019 were selected as the study group, and 60 healthy volunteers were selected as the control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of HPSE and VEGF in serum samples of the two groups. Results The expression levels of serum HPSE and VEGF in the study group were significantly higher than those in the control group, and the differences were statistically significant (P <0.05). The high expression of serum HPSE and VEGF in the study group was closely related to the degree of tumor differentiation, tumor stage, and lymph node metastasis, but not to the patient's gender, age, and tumor location. Tumor size was related to VEGF expression but not to HPSE expression. The receiver operating characteristic curve (ROC curve) value of the combined detection of serum HPSE and VEGF indicators for the diagnosis of colorectal cancer was 0.86, which were higher than the 0.76 of serum HPSE and 0.80 of serum VEGF. There was a significant positive correlation between the expression levels of serum HPSE and VEGF. Conclusion The combined detection of the two indicators can improve the diagnosis of colorectal cancer, and can be used as an indicator for screening patients with colorectal cancer, judging the prognosis and guiding treatment. [ABSTRACT FROM AUTHOR]

Objective To establish a sort of direct competitive ELISA based on glass capillary for rapid and accurate detection of HBsAg. Methods In this study, the amino groups were modified to the surface of glass capillaries after a series treatment, following the HBsAg is coupled to the glass capillaries by carbodiimide method. The HRP labeled anti HBsAg antibody was prepared by the sodium periodate method, then the direct competitive ELISA was established by the modified glass capillaries for rapid and accurate detection of HBsAg. Results The linear range of this method was from 1 to 300 ng/mL (R2 = 0.996) and the limit of detection was 0.13 ng/mL. Conclusion This method has a high precision and precise, in addition it has high specificity in detecting HBsAg in serum. Therefore, the direct competitive ELISA based on glass capillaries could complete the rapid and accurate detection of HBsAg in serum, which shows a great potential in the clinic detection of HBsAg. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To study the expression level of long chain non coding RNA MIR155HG in serum of patients with chronic obstructive pulmonary disease (COPD) in different periods, and to explore the correlation between MIR155HG and lung function in different periods of COPD. Methods A total of 104 patients with COPD who were treated and treated in the hospital from June 2017 to July 2019 were selected as the research subjects. Among them, 5 6 patients were acute exacerbation phase COPD (acute exacerbation phase group), and 4 8 patients were stable phase COPD (stabilization phase group). During the same period,46 healthy people were selected as the control group. The qRT PCR was used to detect the expression level of MIR155HG in the ser um of the subjects, the level of lung function index of each group was detected, and the correlation between the level of serum MIR155HG and the index of lung function in different periods of COPD was analyzed. Results The expression level of serum MIR155HG in the acute exacerbation group was significantly higher than that in the stable phase group and the control group,the differences were statistically significant (P<0. 05). The expression level of serum MIR155HG in the stable group was significantly higher than that in the control group,and the difference was statistically significant (P<0. 05). FEVl, FVC, FEVl/FVC,PEF,and MMEF in the acute exacerbation group were significantly lower than those in the stable group and the control group,and the differ ences were statistically significant (P<0. 05). The levels of FEVl, FVC, FEVl/FVC, MMEF, and PEF in the stable group were significantly lower than those in the control group,and the differences were statistically significant (P<0. 05). Serum MIR155 HG levels of COPD patients in stable and acute exacerbation periods were negatively correlated with FEVl, FVC,FEVl/FVC, MMEF, and PEF levels (P<0. 05), Conclusion The changes of serum MIR155 HG levels in patients with COPD in stable and acute exac erbations are closely related to lung function. Serum MIR155HG combined with lung function index is of great significance for eval uating the early risk of COPD. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的对药物性肝损伤 (DILI) 患者的肝组织铁沉积与血清生物化学和病理学的关系进行观察及分析。方法选择 2015 年 1 月至 2018 年 6 月该院收治的 106 例 DILI 患者为研究对象。根据肝穿刺组织铁染色结果将患者分成铁沉积阳性组 (n=43)、铁沉积阴性组 (n=63)。比较两组血清生物化学、血清铁、铁蛋白和肝组织病理学评分情况。结果铁染色阳性组患者的血清生物化学(谷丙转氨酶、谷草转氨酶、γ-谷氨酰转肽酶、总胆红素)、血清铁、血清铁蛋白指标水平均高于铁染色阴性组,差异均有统计学意义 (P<0.05); 另外,两组的 Ishak 评分界面炎性反应、电灶状坏死、胆管内胆汁淤积、肝细胞内胆汁淤积、肝内胆管增生等指标比较,差异均有统计学意义 (P<0.05)。结论肝组织铁沉积阳性的 DILI 患者产生肝脏生物化学指标增加的情况下,密切地关联于组织炎性反应状态、胆汁淤积情况两项因素。 [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of the Chinese Society of Animal Science is the property of Chinese Society of Animal Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)