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[Objectives] Heterogeneous nuclear ribonucleoproteins (hnRNP) represent a large family of RNA-binding proteins that contribute to multiple aspects of RNA metabolism. In Fusarium graminearum, FGSG_07478 was identified as the homologous protein of annotated Fusarium hnRNP G. The objective of this study was to analyze the function of FGSG_07478 in growth and development, stress, mycotoxins production and pathogenicity of F.graminearum. [Methods] Based on homologous recombination and polyethylene glycol (PEG) mediated protoplast transformation, the FGSG_07478 gene knockout mutant strain ΔFGSG_07478 was obtained to investigate its function in mycelial growth, asexual and sexual reproduction, and stress resistance of F.graminearum. Pathogenicity of the mutant ΔFGSG_07478 was assessed by infection assays using wheat coleoptiles. Deoxynivalenol (DON) produced by ΔFGSG_07478 was quantified using the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method, and the relative expression levels of seven TRI involved in DON biosynthesis were determined by RT-qPCR. [Results] Compared with the wild type strain PH-1, the growth rate of ΔFGSG_07478 was reduced by 16.2%, and the spore production decreased by 43.4%. In mating cultures, the ΔFGSG_07478 mutant produced less perithecia, verifying the weakened sexual reproduction. External environmental stress assays showed that ΔFGSG_07478 mutant became sensitive to H2O2 and SDS, but more resistant to Congo red. Although no significant difference in pathogenicity, DON biosynthesis and expression levels of TRI4, TRI5, TRI6, TRI8, TRI10, TRI12 and TRI101 were significantly reduced in ΔFGSG_07478 mutant compared with the wild type. [Conclusions] FGSG_07478 played an important role in fungal growth and development, sexual reproduction, responses to various environmental stresses and mycotoxins production in F.graminearum. [ABSTRACT FROM AUTHOR]

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