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Objective: To prepare the anti-herpes simplex virus-1 (HSV-1) egg yolk antibody (IgY) and discuss its biological activity, and to clarify its anti-HSV-1 capability. Methods: The inactivated HSV-1 vaccine was prepared, the high egg-producing hens were immunized by multi-site injection method in the chicken breast, and IgY was purified by polyethylene glycol-6000 (PEG-6000) method; the titer, purity, and protein levels of IgY were detected by indirect ELISA method, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) technology, and protein concentration quantification kits; the ability of IgY to neutralize the virus was detected by indirect ELISA method; the virus blocking rate was detected. The Vero cells were used as the target cells for viral infection. The cytopathic effect (CPE) at different antibody concentrations (0. 015 60, 0. 031 25, 0. 062 50, 0. 125 00, 0. 250 00, 0. 500 00, and 1. 000 00 g·L-1) was detected; the in vitro anti-HSV-1 capacity of IgY was detected according to the degrees of CPE. Results: The titer of the prepared IgY was gradually increased with the extension of immunization time. The titer of the IgY stabilized at 1/1 024 000; the clear bands for the IgY light and heavy chains were observed; the average protein level of IgY was 11. 544 g·L-1; the blocking rate of IgY against HSV-1 could reach up to 77. 90%; the degree of HSV-1 induced CPE was decreased with the increasing of the antibody concentrations, and when the concentration of IgY reached 0. 500 00 g·L-1 or higher, less than 25% of the cells (even no cells) exhibited cytopathic changes. Conclusion: Anti-HSV-1 IgY is successfully prepared, and this antibody has significant neutralizing and blocking capabilities and in vitro inhibitory activity against HSV-1, which can be utilized for the development of drugs and diagnostic methods. [ABSTRACT FROM AUTHOR]

Copyright of Tianjin Medical Journal is the property of Tianjin Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Science Research & Application is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Management & Technology Discussion is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Science Research & Application is the property of Omniscient Pte. Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Treatment strategies for femoral neck fractures include cannulated screw fixation, dynamic hip screw fixation, intramedullary nail fixation, locking plate fixation, and hip replacement. However, the best treatment for Pauwels type III femoral neck fractures is still controversial. OBJECTIVE: To analyze the biomechanical characteristics of a novel femoral neck system for unstable femoral neck fractures by using a three-dimensional finite element analysis method. METHODS: The CT imaging data of the femur of a healthy volunteer were selected and imported into the medical three-dimensional reconstruction software Mimics 21.0 to obtain the original three-dimensional model of the femur. The model was exported to an STL format file and imported into the Geomagic Wrap2017 software for optimization so as to construct femoral cortical bone and cancellous bone structure. The optimized model was imported into Solidworks2017 software, and the 3D model of Pauwels type III femoral neck fracture was reconstructed through Boolean operation. The Pauwels angles are set to 50°, 60° and 70°, respectively. Solidworks2017 software was used to establish three types of internal fixation models (femoral neck system, positive triangular hollow screw, and inverted triangular hollow screw) based on clinical fixation procedures and engineering geometric data modeling. Finally, Abaqus2017 software was used for meshing. After applying load and data calculation, the stress distribution, peak stress and maximum displacement of femur and internal fixation were analyzed in each model. RESULTS AND CONCLUSION: (1) No matter what the angle of the fracture, the stress of the proximal femur in the three internal fixation models was mainly distributed near the medial femoral talus and spread to the surroundings. Among them, the inverted-triangular hollow screw group had the largest stress. The femoral neck system group had the smallest stress. (2) Regardless of the angle of the fracture, the stress of the internal fixation device in the three internal fixation models was mainly distributed on the middle surface of the screw near the fracture line, and the inverted triangle hollow screw group had the least stress, and the femoral neck system group had the largest stress. (3) No matter what the angle of the fracture, the peak displacement of the femur in the three internal fixation models was mainly concentrated on the top of the femoral head. Among them, the peak displacement was the largest in the inverted triangular hollow screw group, and the smallest in the femoral neck system group. (4) No matter what angle of fracture, the peak displacement of the internal fixation device in the three internal fixation models was mainly at the top of the screw. The peak displacement was the largest in the positive triangle hollow screw group, and the smallest in the femoral neck system group. (5) The results show that the biomechanical stability of the femoral neck system for the treatment of unstable femoral neck fractures is better than that of hollow screws, and the risk of internal fixation failure is lower than that of hollow screws. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Intraarticular injection of corticosteroids after arthroscopic shoulder surgery is one of the effective methods to relieve shoulder pain and stiffness after surgery. However, whether the use of corticosteroids will increase the complications associated with arthroscopic shoulder surgery is still controversial. OBJECTIVE: To evaluate the safety and clinical efficacy of corticosteroid injections after arthroscopic shoulder surgery. METHODS: The electronic databases including PubMed, EMBASE and the Cochrane Central Register of Controlled Trials (CENTRAL) were searched for clinical controlled trials of corticosteroid injection into the articular cavity after arthroscopic shoulder surgery from the inception of the databases to September 2019. Data extraction and quality appraisement were performed independently by two investigators according to inclusion and exclusion criteria. Merging and analysis of effect quantities was conducted on RevMan 5.3 software. RESULTS AND CONCLUSION: (1) A total of six comparative studies were included. A total of 7 418 patients were enrolled, of which 3 920 were in the corticosteroid injections group and 3 498 in the control group. (2) Meta-analysis showed that there was no significant difference in tear rate, Constant score, ASES score and UCLA score between the two groups [OR=0.71, 95%CI[0.45, 1.13], P=0.15; MD=-0.99, 95% CI(-12.44, 10.46), P=0.87; MD=-0.12, 95%CI(−1.80, 1.56), P=0.89; MD=−1.46, 95%CI(−3.22, 0.30), P=0.10]. The infection rate of corticosteroid injections group was higher than that of control group (P < 0.05). There was no significant difference in infection rate between the two groups 2-4 months after arthroscopy (P > 0.05). (3) The results showed that the injection of corticosteroids did not increase the rate of postoperative tear, but the injection of corticosteroids within one month after the operation would increase the rate of postoperative infection. Therefore, when using corticosteroids after the operation, clinicians should weigh the advantages and disadvantages according to the actual situation of patients, and pay attention to avoid injecting intraarticular hormone treatment within 1 month after operation. [ABSTRACT FROM AUTHOR]

BACKGROUND: Calcaneal fractures account for approximately 60% of all tarsal fractures, and 75% of calcaneal fractures are intra-articular. Surgical treatment can restore the shape and function of calcaneus well. In recent years, transtarsal sinus incision has been recognized and applied by clinicians, but the choice of surgical approach for calcaneal fractures is still controversial. OBJECTIVE: To systematically evaluate the efficacy and surgical safety of transtarsal sinus incision and traditional L-shaped incision in the treatment of calcaneal fracture. METHODS: PubMed, Cochrane, EMbase, CNKI, VIP and Wanfang databases were searched by computer for all randomized controlled trials comparing transtarsal sinus incision with traditional L-shaped incision in the treatment of calcaneal fractures. The retrieval time was from the establishment of the database to April 2019. The meta-analysis was carried out with RevMan 5.3 software after literature screening and data extraction based on inclusion criteria and exclusion criteria. RESULTS AND CONCLUSION: (1) A total of 522 patients were enrolled in 7 randomized controlled trials. (2) Meta-analysis results showed that there was no significant difference in Bohler angle [MD=0.14, 95%CI (-0.54, 0.81), P > 0.05], Gissane angle [MD=-0.19, 95%CI (-1.36, 0.98), P > 0.05] and Maryland score [MD=-0.25, 95%CI (-3.41, 2.91), P > 0.05] between the two incisions at the last follow-up. (3) American Orthopaedic Foot and Ankle Society score [MD=6.39, 95%CI(-0.09, 12.87), P=0.05] might be different. (4) Compared with the traditional L-shaped incision, the tarsal sinus incision had certain advantages in shortening the operation time [MD=-14.98, 95%CI (-23.90, -6.06), P=0.001 < 0.05], and lower incidence of postoperative complications [OR=0.18, 95%CI (0.08, 0.38), P < 0.000 1]. (5) For Sanders II and III calcaneal fractures, the transtarsal sinus incision has no obvious advantage over the traditional L-shaped incision in terms of curative effect. However, because the transtarsal sinus incision has a relatively small wound, it has the advantages of relatively short operation time and relatively low incidence of complications after operation. This suggests that the tarsal sinus incision is safe. However, due to the small sample size of the included study, the above conclusions still need to be verified by clinical trials with higher quality and larger sample size. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)